##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935786.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 813722 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03905879403531 33.0 31.0 34.0 30.0 34.0 2 32.0646473857165 33.0 31.0 34.0 30.0 34.0 3 32.10980162758288 33.0 31.0 34.0 30.0 34.0 4 35.77103482516142 37.0 35.0 37.0 35.0 37.0 5 34.82557802296116 37.0 35.0 37.0 33.0 37.0 6 35.31789358036283 37.0 35.0 37.0 33.0 37.0 7 35.8978225978897 37.0 35.0 37.0 35.0 37.0 8 35.90172196401228 37.0 35.0 37.0 35.0 37.0 9 37.76237707718361 39.0 38.0 39.0 35.0 39.0 10 37.37438461784246 39.0 37.0 39.0 34.0 39.0 11 37.26697201255465 39.0 37.0 39.0 34.0 39.0 12 37.1060226957118 39.0 37.0 39.0 34.0 39.0 13 36.958758642386464 39.0 37.0 39.0 33.0 39.0 14 37.85847992311871 40.0 37.0 41.0 33.0 41.0 15 38.02022680964752 40.0 37.0 41.0 33.0 41.0 16 38.10105908406065 40.0 37.0 41.0 33.0 41.0 17 38.15378347887854 40.0 37.0 41.0 34.0 41.0 18 38.13079651281396 40.0 37.0 41.0 34.0 41.0 19 38.09273806041867 40.0 37.0 41.0 34.0 41.0 20 38.09460970700067 40.0 36.0 41.0 34.0 41.0 21 37.958244953436186 40.0 36.0 41.0 33.0 41.0 22 38.046476560791035 40.0 36.0 41.0 34.0 41.0 23 38.06550885929101 40.0 36.0 41.0 34.0 41.0 24 38.01151498914863 40.0 36.0 41.0 34.0 41.0 25 37.85607123808868 40.0 36.0 41.0 34.0 41.0 26 37.874239605172285 40.0 36.0 41.0 34.0 41.0 27 37.88474687915529 40.0 36.0 41.0 34.0 41.0 28 37.807828717915946 40.0 36.0 41.0 34.0 41.0 29 37.76340445508417 40.0 36.0 41.0 33.0 41.0 30 37.650893056842506 40.0 36.0 41.0 33.0 41.0 31 37.5689633560356 40.0 35.0 41.0 33.0 41.0 32 37.52903325705831 40.0 35.0 41.0 33.0 41.0 33 37.40189892862673 40.0 35.0 41.0 33.0 41.0 34 37.255336589154524 40.0 35.0 41.0 32.0 41.0 35 37.21754972828558 39.0 35.0 41.0 32.0 41.0 36 37.15740633779104 39.0 35.0 41.0 32.0 41.0 37 37.114615310880126 39.0 35.0 41.0 32.0 41.0 38 37.03081027672842 39.0 35.0 41.0 32.0 41.0 39 36.968104586087144 39.0 35.0 41.0 32.0 41.0 40 36.83078618987812 39.0 35.0 41.0 31.0 41.0 41 36.69912574564778 39.0 35.0 41.0 31.0 41.0 42 36.74384249166177 39.0 35.0 41.0 31.0 41.0 43 36.68246280670794 39.0 35.0 41.0 31.0 41.0 44 36.552376610193654 39.0 35.0 41.0 31.0 41.0 45 36.52827992852596 39.0 35.0 41.0 31.0 41.0 46 36.41667424501242 38.0 35.0 41.0 31.0 41.0 47 36.306614544033465 38.0 35.0 40.0 31.0 41.0 48 36.25375742575474 38.0 35.0 40.0 31.0 41.0 49 36.23909517009495 38.0 35.0 40.0 31.0 41.0 50 36.121134736433326 38.0 35.0 40.0 31.0 41.0 51 34.90882635592991 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 16.0 9 22.0 10 25.0 11 16.0 12 11.0 13 22.0 14 17.0 15 30.0 16 59.0 17 106.0 18 187.0 19 314.0 20 596.0 21 972.0 22 1365.0 23 2146.0 24 2956.0 25 4177.0 26 5404.0 27 6659.0 28 7854.0 29 9413.0 30 12055.0 31 15668.0 32 20884.0 33 31361.0 34 58022.0 35 67593.0 36 60448.0 37 91156.0 38 157183.0 39 256832.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30769230769231 21.95233753050796 24.503700280931326 11.236269880868404 2 31.998028810822372 24.335215220923118 25.040001376391448 18.62675459186307 3 31.59506563666707 23.44559935702857 27.853370069876444 17.10596493642792 4 29.003517171712208 24.575715047645264 26.18265206053173 20.238115720110798 5 24.973885430159196 31.07670678683875 24.738547071358525 19.210860711643534 6 26.672499944698558 34.32757133271559 24.133180619425307 14.866748103160537 7 78.96923027766239 5.955227952543989 8.700883102582946 6.374658667210668 8 80.41677624544991 5.041893914629321 9.099913729750456 5.441416110170303 9 74.74542902858717 6.553958231435305 11.418518855333886 7.28209388464365 10 43.93711857366521 20.872853382359086 18.47535153283308 16.714676511142628 11 31.304917404224046 24.62585502173961 23.520194857703245 20.549032716333095 12 28.288162296214185 21.120358058403237 28.67416636148463 21.91731328389794 13 26.46985088273391 22.511619447427993 30.818633390764905 20.199896279073197 14 21.638102447764716 26.086427551424197 30.94877611764214 21.326693883168947 15 19.807624717040955 23.600197610486138 36.33009799415525 20.262079678317658 16 24.436969874232233 23.30402766546806 31.034431906719984 21.224570553579724 17 25.369597970805753 22.820324385969656 26.682577096354777 25.127500546869815 18 24.56354873040178 24.48133391993826 30.080297693806973 20.87481965585298 19 25.218440695962503 25.46373331432602 27.676897023799285 21.64092896591219 20 25.432395830517056 26.78285704454347 27.609797940820087 20.17494918411939 21 25.070847291826936 25.912412347214403 29.606179997591315 19.410560363367342 22 24.232354538773684 23.102853308623832 29.02858715875938 23.63620499384311 23 22.927732075573722 25.22102142992324 28.915280648673626 22.93596584582941 24 23.249709360199184 25.801686571089387 27.610412401286926 23.338191667424503 25 24.238867819722216 25.84838556656942 25.786325059418324 24.12642155429004 26 21.080909696431952 26.409387972796605 27.01033030936856 25.499372021402888 27 21.317108299886204 26.256510208646194 28.823234470740623 23.60314702072698 28 19.91761314060576 25.52948058427817 28.653151813518623 25.899754461597453 29 21.114090561641447 27.097337911473453 27.02900990756057 24.759561619324536 30 23.884078346167364 24.99379394928489 26.776466655688306 24.345661048859437 31 23.40467628993686 24.803556988750458 26.532157174071735 25.259609547240952 32 24.663951570683846 26.641776921356435 24.296995779885513 24.39727572807421 33 24.6699732832589 25.145442792501616 24.742602510439685 25.441981413799798 34 20.872976274452455 25.482412912518033 29.344542730809785 24.300068082219724 35 21.770703016509323 28.097310875212912 25.90884847650672 24.223137631771046 36 24.37576961173472 27.08123904724218 25.35349910657448 23.18949223444862 37 21.64240367103261 27.70503931318067 26.53817888664679 24.114378129139926 38 22.18718432093516 28.253383833790902 23.955232868227725 25.604198977046217 39 22.528086987939368 24.993302380911416 27.02347976335899 25.455130867790228 40 23.627602547307312 24.097050343974967 29.184045656870527 23.09130145184719 41 20.60593175556271 26.408773512329763 27.003694136326654 25.98160059578087 42 21.011352771585383 26.34044550841688 27.198478104315726 25.449723615682014 43 22.046718658214967 24.50111954697059 27.14637185672748 26.305789938086967 44 21.831288818539992 24.151368649243846 27.442910478025667 26.5744320541905 45 21.83657317855484 23.502989964631656 26.981573559520328 27.678863297293177 46 23.12227025937605 24.054775463856206 27.667065656329804 25.15588862043794 47 20.355477669277715 24.507632827919117 30.011232337333883 25.12565716546929 48 19.998721922228967 26.423274779347246 27.606111178019027 25.971892120404753 49 22.017347447899898 24.009182497216493 28.988647228414617 24.984822826468992 50 20.970306812400306 24.375155151267876 28.653028921425257 26.00150911490656 51 20.3793187353912 24.692831212625443 26.456947212930217 28.470902839053142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 330.0 1 681.0 2 1032.0 3 1759.0 4 2486.0 5 1782.0 6 1078.0 7 1090.5 8 1103.0 9 1144.5 10 1186.0 11 1163.5 12 1141.0 13 1188.5 14 1236.0 15 1188.0 16 1140.0 17 1170.0 18 1200.0 19 1457.0 20 1714.0 21 1899.5 22 2085.0 23 2366.0 24 2647.0 25 3102.0 26 3798.5 27 4040.0 28 5040.5 29 6041.0 30 8405.5 31 10770.0 32 11742.5 33 12715.0 34 15090.0 35 17465.0 36 19899.0 37 22333.0 38 24260.0 39 26187.0 40 28869.5 41 31552.0 42 37071.0 43 42590.0 44 46444.0 45 50298.0 46 61464.0 47 72630.0 48 79839.0 49 87048.0 50 86109.5 51 85171.0 52 73918.5 53 62666.0 54 57933.5 55 53201.0 56 49595.0 57 45989.0 58 43382.5 59 40776.0 60 36375.5 61 31975.0 62 29155.5 63 26336.0 64 23614.0 65 20892.0 66 18441.0 67 15990.0 68 13416.0 69 10842.0 70 8314.5 71 5787.0 72 4984.0 73 4181.0 74 3103.5 75 1641.5 76 1257.0 77 894.5 78 532.0 79 380.5 80 229.0 81 184.0 82 139.0 83 116.0 84 93.0 85 55.0 86 17.0 87 12.5 88 8.0 89 8.0 90 8.0 91 5.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 813722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.02459209212674 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39599824091545 20.00843727711854 2 11.865905004564565 6.650742951136945 3 4.367246645134976 3.671709173868501 4 2.1285918769455887 2.386116763280582 5 1.2775382855638655 1.790124466750113 6 0.9034819916447236 1.5191828567055385 7 0.6537753112876012 1.2825250493116744 8 0.48218343721111506 1.0810395313136885 9 0.38789233528432876 0.9783472024805195 >10 5.637159699355499 40.46737494012299 >50 0.8310467188573459 14.359128228755857 >100 0.060367656644512796 3.0098846861978354 >500 0.005728317783785886 1.1917780055179827 >1k 0.0030844788066539384 1.6036088674392834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC 3151 0.3872329862041336 No Hit CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC 2364 0.2905169087231266 No Hit GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA 2166 0.2661842742361642 No Hit CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT 1619 0.19896229916359642 No Hit GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT 1336 0.16418383674031178 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.16098864231273088 No Hit TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC 1040 0.12780777710323674 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC 967 0.11883665428733647 No Hit GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC 966 0.11871376219396797 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG 957 0.1176077333536515 No Hit GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCACC 922 0.1133065100857541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2289209336849687E-4 0.0 0.0 0.21002258756676112 0.0 2 1.2289209336849687E-4 0.0 0.0 1.1650170451333501 0.0 3 1.2289209336849687E-4 0.0 0.0 1.6109924519676253 0.0 4 1.2289209336849687E-4 0.0 0.0 2.1426236478797427 0.0 5 1.2289209336849687E-4 0.0 0.0 3.6013527961638006 0.0 6 1.2289209336849687E-4 0.0 0.0 4.375941660665436 0.0 7 1.2289209336849687E-4 0.0 0.0 5.153848611688021 0.0 8 1.2289209336849687E-4 0.0 0.0 6.398008164950683 0.0 9 1.2289209336849687E-4 0.0 0.0 6.866964393244867 0.0 10 1.2289209336849687E-4 0.0 0.0 7.952716038155537 0.0 11 1.2289209336849687E-4 0.0 0.0 9.411690970626331 0.0 12 1.2289209336849687E-4 0.0 0.0 10.585556246482215 0.0 13 2.4578418673699374E-4 0.0 0.0 11.156758696458986 0.0 14 2.4578418673699374E-4 0.0 0.0 11.348347470020475 0.0 15 2.4578418673699374E-4 0.0 0.0 11.77785533634337 0.0 16 2.4578418673699374E-4 0.0 0.0 12.461995620125792 0.0 17 2.4578418673699374E-4 0.0 0.0 13.29360641595041 0.0 18 2.4578418673699374E-4 0.0 0.0 14.266051550775327 0.0 19 2.4578418673699374E-4 0.0 0.0 14.841678116113366 0.0 20 2.4578418673699374E-4 0.0 0.0 15.441883100125104 0.0 21 2.4578418673699374E-4 0.0 0.0 16.155763270502703 0.0 22 2.4578418673699374E-4 0.0 0.0 16.878491671602834 0.0 23 2.4578418673699374E-4 0.0 0.0 17.546778875340717 0.0 24 2.4578418673699374E-4 0.0 0.0 18.121913872305285 0.0 25 2.4578418673699374E-4 0.0 0.0 18.716957388395546 0.0 26 2.4578418673699374E-4 0.0 0.0 19.198694394400054 0.0 27 2.4578418673699374E-4 0.0 0.0 19.742246123368915 0.0 28 2.4578418673699374E-4 0.0 0.0 20.243277188032277 0.0 29 3.686762801054906E-4 0.0 0.0 20.79248195329609 0.0 30 3.686762801054906E-4 0.0 0.0 21.39993757081657 0.0 31 4.915683734739875E-4 0.0 0.0 21.91497833412394 0.0 32 6.144604668424843E-4 0.0 0.0 22.445134824915634 0.0 33 6.144604668424843E-4 0.0 0.0 22.95304784680763 0.0 34 6.144604668424843E-4 0.0 0.0 23.537522642868204 0.0 35 6.144604668424843E-4 0.0 0.0 24.056618845256732 0.0 36 6.144604668424843E-4 0.0 0.0 24.53602090148724 0.0 37 6.144604668424843E-4 0.0 0.0 25.06666896065241 0.0 38 6.144604668424843E-4 0.0 0.0 25.628531611533177 0.0 39 6.144604668424843E-4 0.0 0.0 26.527241490336994 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGAG 35 1.2113378E-7 45.000004 1 CGTCTCA 45 3.8380676E-10 45.0 10 CTAAGCG 25 3.8897975E-5 45.0 1 TAACGCC 25 3.8897975E-5 45.0 12 CCGTATG 20 7.0320716E-4 45.0 29 TCCGTTA 20 7.0320716E-4 45.0 13 TCGAAAT 25 3.8897975E-5 45.0 19 TCAACTT 755 0.0 43.21192 3 CTCAACT 795 0.0 41.88679 2 TACGCGC 60 3.6379788E-12 41.250004 34 AATGTCG 60 3.6379788E-12 41.250004 15 CTTGACC 760 0.0 41.151314 7 CGAAAGG 180 0.0 40.0 2 ACTTGAC 790 0.0 39.588608 6 CAACTTG 805 0.0 39.40994 4 ACGGGTA 120 0.0 39.375004 5 TGATCGT 40 3.456571E-7 39.375 28 TTGACCC 710 0.0 38.978874 8 TACGGGT 70 0.0 38.57143 4 TATAGCG 35 6.2460385E-6 38.57143 1 >>END_MODULE