FastQCFastQC Report
Sat 14 Jan 2017
SRR2935784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010189
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176840.8797182752467554No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC97520.48512851279158326TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT92570.4605039625627242TruSeq Adapter, Index 27 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC72040.35837426232060765TruSeq Adapter, Index 27 (100% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC64340.32006940640904913No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG59640.2966885203331627No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC58930.2931565141387203TruSeq Adapter, Index 27 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG57030.2837046665761279TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT50140.24942928252020083No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC46300.23032660113054046TruSeq Adapter, Index 27 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT43510.21644730918336536TruSeq Adapter, Index 27 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT33770.1679941537835497TruSeq Adapter, Index 27 (100% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT23940.1190932792886639TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC20350.10123426205197622No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC20290.10093578265526276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102650.043.772531
CGACGGT1000.040.528
CGACGTC356.250624E-638.57142618
GTTAGCG1000.038.251
TAACGCG659.094947E-1238.0769271
CGGCGAA601.5643309E-1037.50000431
TCGTTAA301.14022594E-437.50000439
CCTCGCG301.14022594E-437.50000417
TCAATCG301.14022594E-437.50000445
CGTTCTG9300.036.048391
TACGGGA9550.036.0471234
TGCGACG751.8189894E-1236.01
CGAGCAA250.002107483636.043
CGCGGGC1700.035.7352944
AGCGACT3150.035.71428719
GTTTTTT126250.035.447522
CGTTATT3050.035.4098361
CACAACG3500.035.35714312
TACGGCT8100.035.2777757
CGTTTCT12700.035.25591
Produced by FastQC (version 0.11.5)