FastQCFastQC Report
Sat 14 Jan 2017
SRR2935783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943330
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.23925879596747693No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC17990.19070738765861364No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC16470.1745942565168075Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC16180.1715200407068576No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG16050.1701419439644663No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC13360.1416259421411383Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9920.10515938218862964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA800.045.031
CGATATA253.8902726E-545.010
GCGTAAG650.044.9999961
GTTAACG501.0804797E-940.51
CGTTTTT12400.038.104841
ATAACGG601.546141E-1037.4999962
CTAAGCG552.748493E-936.818181
TCGCAAG250.002106917836.01
ACTTCGG250.002106917836.012
CTAACGG250.002106917836.02
GTCACGA250.002106917836.025
ACGCTTA250.002106917836.017
AACTCGT250.002106917836.013
TAATCCG250.002106917836.01
CGTAAGG1450.035.689662
TAAGGGC4500.035.04
GTACGAG458.697716E-735.01
TAACGGG2400.034.68753
TTAACGG1300.034.6153832
TAGGGAC4200.034.2857135
Produced by FastQC (version 0.11.5)