##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1351445
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09963261545975	33.0	31.0	34.0	30.0	34.0
2	32.1598230042658	33.0	31.0	34.0	30.0	34.0
3	32.147245355896835	33.0	31.0	34.0	30.0	34.0
4	35.80643977372368	37.0	35.0	37.0	35.0	37.0
5	34.93320852864896	37.0	35.0	37.0	33.0	37.0
6	35.376139613524785	37.0	35.0	37.0	33.0	37.0
7	35.90653263728824	37.0	35.0	37.0	35.0	37.0
8	35.89995301325618	37.0	35.0	37.0	35.0	37.0
9	37.73086659094525	39.0	38.0	39.0	35.0	39.0
10	37.40863890132414	39.0	37.0	39.0	35.0	39.0
11	37.284242422000155	39.0	37.0	39.0	34.0	39.0
12	37.10004032720533	39.0	37.0	39.0	34.0	39.0
13	36.978388317689586	39.0	37.0	39.0	33.0	39.0
14	37.89916200807284	40.0	37.0	41.0	33.0	41.0
15	38.08990376966876	40.0	37.0	41.0	33.0	41.0
16	38.14758869210364	40.0	37.0	41.0	33.0	41.0
17	38.17053228211285	40.0	37.0	41.0	33.0	41.0
18	38.11916208206771	40.0	37.0	41.0	34.0	41.0
19	38.03252370610716	40.0	37.0	41.0	34.0	41.0
20	37.98963331841104	40.0	36.0	41.0	33.0	41.0
21	37.8900769176696	40.0	36.0	41.0	33.0	41.0
22	37.95011487703902	40.0	36.0	41.0	34.0	41.0
23	37.96727132809696	40.0	36.0	41.0	34.0	41.0
24	37.908295195142976	40.0	35.0	41.0	34.0	41.0
25	37.74377647629019	40.0	35.0	41.0	33.0	41.0
26	37.764409946390714	40.0	35.0	41.0	33.0	41.0
27	37.7505433073488	40.0	35.0	41.0	34.0	41.0
28	37.60185431149621	40.0	35.0	41.0	33.0	41.0
29	37.50562102046328	40.0	35.0	41.0	33.0	41.0
30	37.33981627073244	39.0	35.0	41.0	33.0	41.0
31	37.26162736922331	39.0	35.0	41.0	32.0	41.0
32	37.2013148888782	39.0	35.0	41.0	32.0	41.0
33	37.03355889436862	39.0	35.0	41.0	32.0	41.0
34	36.86999914905897	39.0	35.0	41.0	31.0	41.0
35	36.7740906955148	39.0	35.0	41.0	31.0	41.0
36	36.68265375209498	39.0	35.0	41.0	31.0	41.0
37	36.58880753563778	39.0	35.0	41.0	31.0	41.0
38	36.483812511792934	39.0	35.0	41.0	31.0	41.0
39	36.414829312328656	39.0	35.0	41.0	30.0	41.0
40	36.25665713366064	39.0	35.0	41.0	30.0	41.0
41	36.11066155115451	39.0	35.0	41.0	30.0	41.0
42	36.10359578081239	39.0	35.0	41.0	30.0	41.0
43	36.003664226069134	38.0	35.0	41.0	30.0	41.0
44	35.89377888112354	38.0	35.0	40.0	29.0	41.0
45	35.873863901231644	38.0	35.0	40.0	29.0	41.0
46	35.77802944255963	38.0	35.0	40.0	29.0	41.0
47	35.66651177073429	38.0	35.0	40.0	29.0	41.0
48	35.58389131633178	38.0	35.0	40.0	29.0	41.0
49	35.51878248837355	37.0	35.0	40.0	29.0	41.0
50	35.37051748313842	37.0	34.0	40.0	28.0	41.0
51	34.1312254660752	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	29.0
10	51.0
11	54.0
12	35.0
13	35.0
14	48.0
15	93.0
16	151.0
17	255.0
18	411.0
19	787.0
20	1248.0
21	1999.0
22	3046.0
23	4611.0
24	6745.0
25	9831.0
26	13019.0
27	15427.0
28	16983.0
29	19161.0
30	22358.0
31	28022.0
32	36837.0
33	53652.0
34	98673.0
35	105526.0
36	101341.0
37	151898.0
38	252191.0
39	406653.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88361346558683	21.064934200059938	23.221440754155736	13.830011580197493
2	35.14416050967668	23.06190780978878	24.608622622452263	17.185309058082275
3	32.204640218432864	23.398658472967824	27.752960719822116	16.6437405887772
4	29.459504456341172	24.871674392964568	27.401189097595534	18.26763205309872
5	25.05503368616555	30.360614009449144	25.265179123086774	19.319173181298535
6	26.874197618105068	33.39225791652638	25.253931902519156	14.479612562849395
7	78.87564791759931	6.044123142266241	10.174368916234105	4.905860023900344
8	80.02900598988491	5.284417789847164	9.85426709929002	4.832309120977916
9	73.62785758946904	7.038244249673498	12.173488377255456	7.160409783601997
10	43.8843608137956	22.239232821165494	18.893554676660905	14.982851688377997
11	32.61020611271639	23.898197854888657	24.204240646123225	19.28735538627173
12	29.082056613476688	20.437087709821707	29.912501063676288	20.568354613025317
13	26.74537254568258	20.49317582291547	32.04939897665092	20.712052654751027
14	21.38903174010041	24.63592673027759	32.76189560063488	21.21314592898712
15	21.028454728087343	23.169496353902673	35.078823037563495	20.723225880446485
16	26.04745291151323	23.16157890258205	30.146472849431532	20.64449533647318
17	26.083784393741517	22.85990180880465	28.025409839098153	23.030903958355687
18	26.97039095190703	23.47590911949802	29.131559182948624	20.422140745646328
19	26.958773756978644	24.646877971356584	27.350724594785582	21.043623676879193
20	25.736452463844255	26.126035465742227	27.166625352863043	20.970886717550474
21	26.04412314226624	24.091546455830613	30.125236321122944	19.739094080780202
22	24.21926160517076	22.563404356078124	30.60205927729208	22.61527476145903
23	23.706107166773343	23.41397541150398	30.091938628653036	22.78797879306964
24	23.839815900758076	24.595229550592144	28.32101935335881	23.243935195290966
25	25.460747570193387	24.270984020807358	26.929397792732964	23.338870616266295
26	22.804405654688132	23.865492121395988	27.625837529459208	25.704264694456675
27	22.748613521082987	23.727935654059173	30.126198254460967	23.39725257039687
28	21.860971034707294	24.666042643244822	29.226716588540413	24.246269733507468
29	23.56351904812996	25.23979888193748	28.45028839501423	22.746393674918327
30	25.313423779732062	23.32584751876695	29.554513872188657	21.806214829312328
31	25.471624816400222	24.55194255038126	27.757844381384373	22.21858825183415
32	27.14568480404308	24.16953705108236	26.44295550318363	22.24182264169093
33	25.833829715600707	23.219220907991076	27.824439766324193	23.122509610084023
34	23.53873076595792	24.17767648701945	28.979425725797203	23.30416702122543
35	23.735409136146863	25.559604719392947	27.42072374384455	23.28426240061564
36	26.795837048492537	24.71917096145237	27.204732712023056	21.280259278032034
37	24.17464269726108	26.155337435115744	26.671895637632314	22.998124229990864
38	24.200836882004076	25.784475135873087	26.279056861359507	23.73563112076333
39	24.31996862617421	22.911920203929867	26.695795981338495	26.072315188557432
40	25.599265970868217	22.294506990665546	28.43156769235892	23.674659346107315
41	23.435877893661967	23.018028850600654	27.88474558713081	25.661347668606567
42	23.847807346950855	23.49056010418478	28.916900058825924	23.74473249003844
43	23.091061789417992	23.289738021155134	29.19571273710732	24.423487452319552
44	23.03882140967631	23.31578421615382	28.454728087343543	25.190666286826325
45	22.931084875818105	22.459737540188467	27.764873894239127	26.8443036897543
46	23.842627705899982	22.98621105557385	28.360236635601154	24.810924602925017
47	22.011846579032074	24.084368953231543	29.807650329832143	24.096134137904244
48	22.49917680704727	24.462704734561893	28.838909463574176	24.199208994816658
49	24.07178982496513	22.94233209638572	29.236779891153542	23.74909818749561
50	22.077702015250345	22.874848772980034	29.83081072481677	25.216638486952853
51	22.351187062736553	23.613095612474055	28.13862199349585	25.897095331293542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	691.0
1	1455.5
2	2220.0
3	7317.0
4	12414.0
5	8135.0
6	3856.0
7	3824.0
8	3792.0
9	3799.5
10	3807.0
11	3841.5
12	3876.0
13	3784.5
14	3693.0
15	3721.5
16	3750.0
17	3600.0
18	3450.0
19	3536.0
20	3622.0
21	3872.5
22	4123.0
23	4345.5
24	4568.0
25	5585.0
26	7685.5
27	8769.0
28	10076.0
29	11383.0
30	13951.0
31	16519.0
32	19087.5
33	21656.0
34	25459.5
35	29263.0
36	31942.0
37	34621.0
38	37577.5
39	40534.0
40	44655.0
41	48776.0
42	53058.0
43	57340.0
44	62488.5
45	67637.0
46	74916.5
47	82196.0
48	96350.5
49	110505.0
50	114735.5
51	118966.0
52	118895.5
53	118825.0
54	108848.0
55	98871.0
56	93537.0
57	88203.0
58	82856.5
59	77510.0
60	73259.0
61	69008.0
62	62571.5
63	56135.0
64	49960.0
65	43785.0
66	38421.0
67	33057.0
68	27929.5
69	22802.0
70	18659.0
71	14516.0
72	11968.0
73	9420.0
74	7216.5
75	3926.0
76	2839.0
77	2176.5
78	1514.0
79	1073.5
80	633.0
81	503.5
82	374.0
83	285.0
84	196.0
85	130.0
86	64.0
87	41.5
88	19.0
89	20.5
90	22.0
91	12.5
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1351445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.130162261243193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5892210217367	20.70076728019942
2	9.640039468113509	5.42351748885643
3	3.482381289191055	2.9387985216138484
4	1.8735832047549055	2.1081679823878208
5	1.1545062739282008	1.6238224408611786
6	0.8232280921089028	1.3894523885422254
7	0.6621841887010452	1.3039144072493059
8	0.5337743634068471	1.201212756282112
9	0.481833588613755	1.2198651327559826
>10	7.238563585176496	48.808746203767235
>50	0.4663995507048014	7.935684079681307
>100	0.047332567108780525	2.5433387059248216
>500	0.002674156328882811	0.5244648754634119
>1k	0.004011234493324216	1.7108296991773346
>5k	2.674156328882811E-4	0.5674180372375955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7543	0.5581433206678776	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2369	0.17529385213604698	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2116	0.15657314948074097	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	2049	0.15161549304633187	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	1725	0.12764115446799537	No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	1662	0.12297947752220771	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	1620	0.11987169289168262	Illumina Single End Adapter 2 (95% over 21bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1413	0.10455475435552317	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	1369	0.10129897998068735	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG	1362	0.10078101587559982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6997436077679816E-4	0.0	0.0	0.12771514934015074	0.0
2	3.6997436077679816E-4	0.0	0.0	0.5646548694175494	0.0
3	3.6997436077679816E-4	0.0	0.0	0.8945240094861426	0.0
4	3.6997436077679816E-4	0.0	0.0	1.256728908686628	0.0
5	3.6997436077679816E-4	0.0	0.0	2.2361990314071236	0.0
6	3.6997436077679816E-4	0.0	0.0	3.076780779092009	0.0
7	3.6997436077679816E-4	0.0	0.0	3.742808623362401	0.0
8	4.439692329321578E-4	0.0	0.0	4.667004576582843	0.0
9	4.439692329321578E-4	0.0	0.0	5.169281768773424	0.0
10	4.439692329321578E-4	0.0	0.0	6.080454624494522	0.0
11	4.439692329321578E-4	0.0	0.0	7.123930311629404	0.0
12	4.439692329321578E-4	0.0	0.0	7.9888563722534025	0.0
13	5.179641050875175E-4	0.0	0.0	8.364306353569697	0.0
14	5.919589772428771E-4	0.0	0.0	8.576301662294803	0.0
15	5.919589772428771E-4	0.0	0.0	8.843349155903496	0.0
16	5.919589772428771E-4	0.0	0.0	9.307075019701134	0.0
17	6.659538493982367E-4	0.0	0.0	9.813125950371639	0.0
18	6.659538493982367E-4	0.0	0.0	10.421511789233007	0.0
19	7.399487215535963E-4	0.0	0.0	10.798663652608875	0.0
20	7.399487215535963E-4	0.0	0.0	11.200455808412476	0.0
21	7.399487215535963E-4	0.0	0.0	11.667881415817885	0.0
22	7.399487215535963E-4	0.0	0.0	12.159207366929472	0.0
23	7.399487215535963E-4	0.0	0.0	12.612203974264583	0.0
24	7.399487215535963E-4	0.0	0.0	12.990243776106317	0.0
25	7.399487215535963E-4	0.0	0.0	13.339129598318836	0.0
26	7.399487215535963E-4	0.0	0.0	13.691419184650504	0.0
27	7.399487215535963E-4	0.0	0.0	14.120145473918658	0.0
28	8.139435937089559E-4	0.0	0.0	14.496557388572972	0.0
29	8.139435937089559E-4	0.0	0.0	14.915146380355841	0.0
30	8.139435937089559E-4	0.0	0.0	15.388713562150143	0.0
31	9.619333380196753E-4	0.0	0.0	15.773412902485857	0.0
32	9.619333380196753E-4	0.0	0.0	16.158852191543126	0.0
33	0.001035928210175035	0.0	0.0	16.57899507564126	0.0
34	0.001035928210175035	0.0	0.0	17.016452759823743	0.0
35	0.0011099230823303945	0.0	0.0	17.462789828664874	0.0
36	0.0011099230823303945	0.0	0.0	17.851706876713443	0.0
37	0.0011099230823303945	0.0	0.0	18.267706047970876	0.0
38	0.0011099230823303945	0.0	0.0	18.776568783783283	0.0
39	0.0011839179544857541	0.0	0.0	19.581854977450064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	30	2.165787E-6	45.000004	1
TCGAACG	30	2.165787E-6	45.000004	1
ATACGGA	240	0.0	43.125004	4
CGTTTTT	3615	0.0	42.074688	1
CCATACG	250	0.0	41.4	2
TCGTTAG	50	1.0822987E-9	40.500004	1
TATAGCG	45	1.9286745E-8	40.0	1
CATCGAT	30	1.14002796E-4	37.500004	19
TACGGGC	140	0.0	36.964283	4
TTACGGG	385	0.0	36.81818	3
CATACGG	300	0.0	36.750004	3
TTAACGG	125	0.0	36.000004	2
TATCGCG	25	0.0021072389	36.000004	1
TACGGAA	290	0.0	35.689655	5
GCGTAAG	120	0.0	35.625004	1
ACCCGAT	175	0.0	34.714287	41
CCCGCAT	300	0.0	34.500004	23
CCGAACC	315	0.0	34.285713	18
CGGGATA	145	0.0	34.137928	6
CGAACCC	310	0.0	34.112904	19
>>END_MODULE