##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495272
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.088420100470046	33.0	31.0	34.0	30.0	34.0
2	32.112081845935165	33.0	31.0	34.0	30.0	34.0
3	32.13606664620653	33.0	31.0	34.0	30.0	34.0
4	35.78564909786945	37.0	35.0	37.0	35.0	37.0
5	34.84400491043306	37.0	35.0	37.0	33.0	37.0
6	35.35191773409359	37.0	35.0	37.0	33.0	37.0
7	35.91086715986367	37.0	35.0	37.0	35.0	37.0
8	35.942183285144324	37.0	35.0	37.0	35.0	37.0
9	37.772617874622426	39.0	38.0	39.0	35.0	39.0
10	37.379900337592275	39.0	37.0	39.0	34.0	39.0
11	37.32725653782164	39.0	37.0	39.0	34.0	39.0
12	37.18892043160122	39.0	37.0	39.0	34.0	39.0
13	37.087949651908446	39.0	37.0	39.0	33.0	39.0
14	38.17144518567575	40.0	37.0	41.0	33.0	41.0
15	38.34021911192234	40.0	37.0	41.0	34.0	41.0
16	38.35064772488653	40.0	37.0	41.0	34.0	41.0
17	38.36174465748114	40.0	37.0	41.0	34.0	41.0
18	38.33843221502528	40.0	37.0	41.0	34.0	41.0
19	38.29922143791694	40.0	37.0	41.0	34.0	41.0
20	38.29822400620265	40.0	37.0	41.0	34.0	41.0
21	38.19456985252548	40.0	37.0	41.0	34.0	41.0
22	38.22358421231162	40.0	37.0	41.0	34.0	41.0
23	38.23579568398779	40.0	37.0	41.0	34.0	41.0
24	38.20158619909868	40.0	37.0	41.0	34.0	41.0
25	38.03506961831074	40.0	36.0	41.0	34.0	41.0
26	38.10297977676913	40.0	36.0	41.0	34.0	41.0
27	38.1000722835129	40.0	36.0	41.0	34.0	41.0
28	37.990645544266584	40.0	36.0	41.0	34.0	41.0
29	37.94964383207611	40.0	36.0	41.0	34.0	41.0
30	37.82056526514723	40.0	36.0	41.0	34.0	41.0
31	37.790896719378445	40.0	36.0	41.0	34.0	41.0
32	37.76547836340435	40.0	36.0	41.0	33.0	41.0
33	37.6344675249156	40.0	36.0	41.0	33.0	41.0
34	37.58352985834047	40.0	36.0	41.0	33.0	41.0
35	37.51453342809608	40.0	36.0	41.0	33.0	41.0
36	37.4839966725355	40.0	35.0	41.0	33.0	41.0
37	37.441097013358316	40.0	35.0	41.0	33.0	41.0
38	37.34357080553716	40.0	35.0	41.0	33.0	41.0
39	37.292205091343746	40.0	35.0	41.0	33.0	41.0
40	37.18319226606794	39.0	35.0	41.0	32.0	41.0
41	37.081926698864464	39.0	35.0	41.0	32.0	41.0
42	37.113967678366635	39.0	35.0	41.0	32.0	41.0
43	37.019451937521204	39.0	35.0	41.0	32.0	41.0
44	36.91049968502156	39.0	35.0	41.0	32.0	41.0
45	36.870737695650064	39.0	35.0	41.0	32.0	41.0
46	36.70884079859148	39.0	35.0	41.0	31.0	41.0
47	36.64545340742057	39.0	35.0	41.0	31.0	41.0
48	36.61150640456153	39.0	35.0	41.0	31.0	41.0
49	36.55780661939298	39.0	35.0	41.0	31.0	41.0
50	36.40747104621299	39.0	35.0	41.0	31.0	41.0
51	35.28130401072542	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	11.0
11	17.0
12	16.0
13	9.0
14	17.0
15	24.0
16	29.0
17	69.0
18	91.0
19	154.0
20	299.0
21	522.0
22	822.0
23	1171.0
24	1629.0
25	2349.0
26	3046.0
27	3758.0
28	4416.0
29	5203.0
30	6459.0
31	8241.0
32	10830.0
33	15911.0
34	30998.0
35	43151.0
36	34281.0
37	52044.0
38	96377.0
39	173193.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87739262465877	22.33399021143937	24.283222148637517	11.50539501526434
2	31.174788802920418	24.34359301555509	25.77936972007301	18.702248461451486
3	31.50248752200811	23.807120127929704	27.487320098854774	17.20307225120742
4	28.482126992844336	26.485042562470724	26.13937391978549	18.89345652489945
5	24.602844497569013	30.85112826891082	25.616832770679547	18.92919446284062
6	25.426836162755013	33.8307031287858	25.163142677155182	15.579318031304009
7	79.3002632896671	6.304414543927376	9.190101600736565	5.205220565668966
8	80.41096609539808	4.8712626597102195	8.543991988240805	6.1737792566508904
9	73.72554878935212	7.60208531877433	11.35376116558174	7.318604726291815
10	40.52944644558949	26.1153467185708	19.127267440921354	14.227939394918348
11	29.240094332003423	26.464649727826327	24.505322327932934	19.789933612237316
12	26.135335734707393	22.052932530003716	31.17115443635013	20.640577298938766
13	23.513544072751944	25.576450919898562	30.93370915375793	19.976295853591562
14	19.037619732187565	29.74284837422669	29.72366699510572	21.495864898480026
15	18.173650034728393	25.577056647660275	36.14761181734481	20.10168150026652
16	19.786904973428744	26.419422054951625	31.64927554959699	22.144397422022646
17	20.875599670484096	24.745594340079794	28.502519827488733	25.876286161947377
18	21.680208047295224	25.475092474438288	31.156011242307258	21.68868823595923
19	22.958697443021208	26.01540163788787	28.24528743801386	22.780613481077065
20	23.51879371335347	26.648589058133716	28.726639099323197	21.105978129189616
21	23.186249172172058	26.398019674037698	30.304963737098	20.11076741669224
22	21.403592369445477	26.178140496535235	27.700738180232275	24.71752895378701
23	20.705390169442246	26.557124166114782	28.158668368088648	24.578817296354327
24	21.655978936826635	25.251982748873345	29.03899271511412	24.0530455991859
25	19.939952187888675	27.245028994168862	28.04358009336284	24.771438724579625
26	18.691143452486713	27.249269088500867	29.425043208580338	24.634544250432086
27	19.491107916458027	26.97144195512769	31.015078583081618	22.522371545332664
28	17.608506033048506	25.79330145859245	33.40164596423783	23.19654654412121
29	18.50982894248009	23.946033694616293	32.55948246620039	24.984654896703226
30	21.04742444555719	24.814849214169186	31.03325041593306	23.104475924340566
31	22.758605372401426	26.03074674118464	28.16977337705342	23.040874509360513
32	21.48132743219887	27.399287664152222	27.517808396194415	23.60157650745449
33	21.440945581417886	26.751966596133038	27.24341372013762	24.563674102311456
34	20.01728343213426	25.42037506663005	29.734368185562683	24.827973315673006
35	18.645713870358104	26.84666203621444	28.942883910255375	25.564740183172074
36	21.856474825954223	25.710720573745338	30.312636288746386	22.120168311554057
37	20.164677187484855	26.509675491447126	31.548724741152334	21.776922579915684
38	18.702248461451486	28.76722285935809	27.11984525674781	25.410683422442617
39	21.787623770372644	25.4674199227899	28.31736904165792	24.427587265179536
40	23.635497262110515	23.937149687444474	28.69756416676089	23.729788883684115
41	19.646779951218722	25.899505726146444	28.287890290587796	26.165824032047034
42	20.51155728569352	25.54232825598863	29.6491624804148	24.29695197790305
43	21.497480172511267	24.26908850086417	30.039453068213025	24.193978258411537
44	19.494944192282222	24.613141869518163	29.55244794779434	26.33946599040527
45	21.239440146020772	24.012058020643202	27.756061315802228	26.9924405175338
46	22.927401508665945	24.244859390395582	29.13086142564086	23.696877675297614
47	19.77660760147959	24.691078841525467	30.897163578801145	24.6351499781938
48	19.14241063496422	24.402954336203138	30.88827957162933	25.566355457203315
49	20.735272739020175	22.479162964997013	32.126992844336044	24.658571451646772
50	20.768385856660583	22.808880776623756	31.2931076257087	25.12962574100696
51	20.5969649000953	23.05056615354795	27.886494693824808	28.465974252531943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	419.0
1	552.5
2	686.0
3	1200.0
4	1714.0
5	1229.5
6	745.0
7	746.0
8	747.0
9	768.0
10	789.0
11	861.5
12	934.0
13	886.0
14	838.0
15	877.0
16	916.0
17	911.5
18	907.0
19	1026.5
20	1146.0
21	1278.0
22	1410.0
23	1644.0
24	1878.0
25	2257.5
26	3235.5
27	3834.0
28	4851.5
29	5869.0
30	5757.0
31	5645.0
32	6803.0
33	7961.0
34	9841.5
35	11722.0
36	12331.0
37	12940.0
38	15164.5
39	17389.0
40	21220.5
41	25052.0
42	29750.0
43	34448.0
44	42043.0
45	49638.0
46	55433.5
47	61229.0
48	60181.5
49	59134.0
50	49946.5
51	40759.0
52	35525.0
53	30291.0
54	28122.5
55	25954.0
56	23602.0
57	21250.0
58	19843.0
59	18436.0
60	16659.5
61	14883.0
62	13091.5
63	11300.0
64	9708.5
65	8117.0
66	6731.5
67	5346.0
68	4456.5
69	3567.0
70	2816.0
71	2065.0
72	1714.5
73	1364.0
74	1056.0
75	505.0
76	262.0
77	204.5
78	147.0
79	108.5
80	70.0
81	51.0
82	32.0
83	37.5
84	43.0
85	23.5
86	4.0
87	2.0
88	0.0
89	1.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.72365434809889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31863928018278	22.218928761691828
2	11.783166834715132	7.240438899115404
3	4.5032271075358485	4.150667793087616
4	2.1491535630882757	2.6411940485323724
5	1.2857388758121349	1.9751298401182635
6	0.8340344240061326	1.5374751214548101
7	0.6033103901481511	1.2975129924069935
8	0.43312074155122965	1.06456415635298
9	0.3709721605334219	1.0257858389696677
>10	5.008669966186512	39.22782564307744
>50	0.6462011324855034	12.214545370921858
>100	0.05784913495292401	3.2494968413241696
>500	0.003944259201335728	0.8230281327861265
>1k	0.001972129600667864	1.3334065601604927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2601	0.5251659694067099	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2464	0.4975044016217351	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1537	0.3103345232518697	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.18252596553005218	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	867	0.17505532313556996	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	641	0.12942383175305688	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	640	0.129221922499152	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	512	0.10337753799932159	No Hit
GCTAGTAGGGACCCACTGTTTGTTTATTCATGTCAGCTGTTCAAATGCAAA	511	0.10317562874541666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.057277617147749E-4	0.0	0.0	0.32244907848616516	0.0
2	8.076370156196999E-4	0.0	0.0	1.7156229304301476	0.0
3	8.076370156196999E-4	0.0	0.0	2.3480027136603723	0.0
4	8.076370156196999E-4	0.0	0.0	3.0710397518939088	0.0
5	8.076370156196999E-4	0.0	0.0	4.900741410780339	0.0
6	8.076370156196999E-4	0.0	0.0	5.86627146295369	0.0
7	8.076370156196999E-4	0.0	0.0	6.804543765849877	0.0
8	8.076370156196999E-4	0.0	0.0	8.250819751570853	0.0
9	8.076370156196999E-4	0.0	0.0	8.77416853769242	0.0
10	8.076370156196999E-4	0.0	0.0	10.005007349496843	0.0
11	8.076370156196999E-4	0.0	0.0	11.768078954594648	0.0
12	8.076370156196999E-4	0.0	0.0	13.216979760616388	0.0
13	8.076370156196999E-4	0.0	0.0	13.92830606212344	0.0
14	8.076370156196999E-4	0.0	0.0	14.282656802726583	0.0
15	8.076370156196999E-4	0.0	0.0	14.717367426383886	0.0
16	8.076370156196999E-4	0.0	0.0	15.617276971038137	0.0
17	8.076370156196999E-4	0.0	0.0	16.693857112859195	0.0
18	0.001009546269524625	0.0	0.0	18.05169684536982	0.0
19	0.001009546269524625	0.0	0.0	18.772714791064303	0.0
20	0.001009546269524625	0.0	0.0	19.45153370269266	0.0
21	0.001009546269524625	0.0	0.0	20.30298502640973	0.0
22	0.001009546269524625	0.0	0.0	21.157868807443183	0.0
23	0.001009546269524625	0.0	0.0	21.99619602965643	0.0
24	0.001009546269524625	0.0	0.0	22.662900386050495	0.0
25	0.001009546269524625	0.0	0.0	23.248235313120873	0.0
26	0.001009546269524625	0.0	0.0	23.7946017541876	0.0
27	0.001009546269524625	0.0	0.0	24.373273675879112	0.0
28	0.001009546269524625	0.0	0.0	24.92085157246927	0.0
29	0.001009546269524625	0.0	0.0	25.483976481610107	0.0
30	0.001009546269524625	0.0	0.0	26.10403980035213	0.0
31	0.001009546269524625	0.0	0.0	26.714209565652812	0.0
32	0.001009546269524625	0.0	0.0	27.281776478379555	0.0
33	0.001009546269524625	0.0	0.0	27.842882294981344	0.0
34	0.001009546269524625	0.0	0.0	28.400555654266746	0.0
35	0.001009546269524625	0.0	0.0	29.004668141950283	0.0
36	0.001009546269524625	0.0	0.0	29.53791048151319	0.0
37	0.001009546269524625	0.0	0.0	30.10689075901727	0.0
38	0.001009546269524625	0.0	0.0	30.647805650228563	0.0
39	0.001009546269524625	0.0	0.0	31.22991002923646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	55	1.8189894E-12	45.000004	2
CCCGCAT	30	2.1629894E-6	45.000004	23
CGGGTCG	25	3.88758E-5	45.0	6
TCGTTTG	25	3.88758E-5	45.0	1
CAGCGCG	20	7.0293946E-4	45.0	1
AGATCGG	40	6.8012014E-9	45.0	2
CACGGAC	20	7.0293946E-4	45.0	42
GCAACGT	20	7.0293946E-4	45.0	17
CAACCCG	45	3.8380676E-10	45.0	23
GACCCGT	40	6.8012014E-9	45.0	41
TTAAGCG	25	3.88758E-5	45.0	1
CCATACG	20	7.0293946E-4	45.0	2
GACGGTC	35	1.2100645E-7	45.0	29
CTCGCTA	25	3.88758E-5	45.0	21
CTCGCGG	20	7.0293946E-4	45.0	2
CTCGAGG	20	7.0293946E-4	45.0	2
CGTAAGT	20	7.0293946E-4	45.0	20
CGTAAAT	20	7.0293946E-4	45.0	16
ATAACGG	40	6.8012014E-9	45.0	2
ACGTAGG	25	3.88758E-5	45.0	2
>>END_MODULE