##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513944
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90938117771586	33.0	31.0	34.0	30.0	34.0
2	31.94104610619056	33.0	31.0	34.0	30.0	34.0
3	31.975649097956197	33.0	31.0	34.0	30.0	34.0
4	35.655688168360754	37.0	35.0	37.0	33.0	37.0
5	34.75386812571019	37.0	35.0	37.0	33.0	37.0
6	35.24377753218249	37.0	35.0	37.0	33.0	37.0
7	35.844090017589465	37.0	35.0	37.0	35.0	37.0
8	35.85774325607459	37.0	35.0	37.0	35.0	37.0
9	37.69212988185483	39.0	37.0	39.0	35.0	39.0
10	37.29157845991003	39.0	37.0	39.0	34.0	39.0
11	37.213604984200614	39.0	37.0	39.0	34.0	39.0
12	37.02215221891879	39.0	37.0	39.0	34.0	39.0
13	36.90432420652834	39.0	37.0	39.0	33.0	39.0
14	37.91447706364896	40.0	37.0	41.0	33.0	41.0
15	38.07461318742898	40.0	37.0	41.0	33.0	41.0
16	38.09357634294787	40.0	37.0	41.0	34.0	41.0
17	38.07923236772878	40.0	36.0	41.0	34.0	41.0
18	38.0911266597139	40.0	37.0	41.0	34.0	41.0
19	38.032653363012315	40.0	36.0	41.0	34.0	41.0
20	38.03766169076786	40.0	36.0	41.0	34.0	41.0
21	37.93301021122924	40.0	36.0	41.0	34.0	41.0
22	37.98260705446508	40.0	36.0	41.0	34.0	41.0
23	37.99918084460564	40.0	36.0	41.0	34.0	41.0
24	37.96836814905904	40.0	36.0	41.0	34.0	41.0
25	37.801661270488616	40.0	36.0	41.0	34.0	41.0
26	37.85654662764815	40.0	36.0	41.0	34.0	41.0
27	37.86960447052597	40.0	36.0	41.0	34.0	41.0
28	37.77904985757203	40.0	36.0	41.0	34.0	41.0
29	37.77738625219868	40.0	36.0	41.0	34.0	41.0
30	37.60724514733123	40.0	36.0	41.0	33.0	41.0
31	37.56546822221876	40.0	35.0	41.0	33.0	41.0
32	37.515563952492876	40.0	35.0	41.0	33.0	41.0
33	37.27810422925455	39.0	35.0	41.0	33.0	41.0
34	37.25000778294911	39.0	35.0	41.0	33.0	41.0
35	37.22210591037156	39.0	35.0	41.0	33.0	41.0
36	37.0671143159566	39.0	35.0	41.0	32.0	41.0
37	37.05946367697648	39.0	35.0	41.0	32.0	41.0
38	36.96057547125757	39.0	35.0	41.0	32.0	41.0
39	36.87299005339103	39.0	35.0	41.0	31.0	41.0
40	36.80246680572203	39.0	35.0	41.0	31.0	41.0
41	36.711293448313434	39.0	35.0	41.0	31.0	41.0
42	36.69076397428513	39.0	35.0	41.0	31.0	41.0
43	36.55227028625687	39.0	35.0	41.0	31.0	41.0
44	36.445527139143564	39.0	35.0	40.0	31.0	41.0
45	36.433045234500256	39.0	35.0	40.0	31.0	41.0
46	36.28760915586134	39.0	35.0	40.0	31.0	41.0
47	36.16078989150569	38.0	35.0	40.0	30.0	41.0
48	36.1143412511869	38.0	35.0	40.0	30.0	41.0
49	36.0543658453061	38.0	35.0	40.0	30.0	41.0
50	35.919750011674424	38.0	35.0	40.0	30.0	41.0
51	34.6576183397413	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	7.0
11	16.0
12	13.0
13	7.0
14	15.0
15	13.0
16	32.0
17	53.0
18	92.0
19	233.0
20	363.0
21	606.0
22	903.0
23	1345.0
24	1936.0
25	2710.0
26	3500.0
27	4458.0
28	5063.0
29	6220.0
30	7720.0
31	9886.0
32	13101.0
33	19285.0
34	35727.0
35	47600.0
36	38915.0
37	59197.0
38	104833.0
39	150013.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.500933953893814	21.038673474152823	25.056037233628565	11.404355338324798
2	31.013106486309795	25.1385364942484	25.747163115047556	18.101193904394254
3	31.78556418598135	24.282217517861866	27.571097240166242	16.361121055990534
4	29.158235138458664	24.87683483025388	26.61612938374609	19.348800647541367
5	23.98763289385614	31.2884283112557	26.08533225409772	18.638606540790438
6	24.58108276388089	33.643354139750635	26.204216801830547	15.571346294537927
7	77.83980355836434	6.131407312858989	10.593177466805722	5.435611661970954
8	78.14547888485906	5.0600843671684075	9.853019005961738	6.9414177420108025
9	72.41859035225627	7.1383263546222935	12.68426132029949	7.758821972821941
10	40.74218202761391	24.32716404900145	20.017355976526627	14.913297946858023
11	30.115537879613342	25.112463614712887	24.430093551048362	20.341904954625406
12	26.415523870304934	22.202029793129213	31.407507432716404	19.974938903849445
13	22.792561057235808	26.950796195694473	31.825646373924005	18.43099637314571
14	19.40055725915664	29.20084678486372	30.36731628348614	21.031279672493504
15	18.457652973864857	25.645595629095773	36.415056893358035	19.481694503681336
16	19.89321793814112	26.065291160126396	30.9936491135221	23.047841788210388
17	19.560107716015754	25.40607537007923	28.56361782606665	26.470199087838363
18	21.750229596998892	25.24633033949224	31.67777034073751	21.325669722771355
19	22.00901265507526	26.405211462727458	28.57101162772598	23.014764254471302
20	23.291058948056598	26.608735582086762	29.337826689289102	20.762378780567534
21	22.620168734336815	27.200045141104866	30.49573494388494	19.684051180673382
22	21.151137088865713	25.42203041576514	28.24607350217144	25.180758993197706
23	19.98583503261056	26.679560419033983	28.191398284638012	25.14320626371745
24	22.657916037544947	23.940546051709912	29.60614386003144	23.795394050713696
25	19.293152561368554	26.5256526002833	28.142365705213017	26.038829133135128
26	20.32945223604128	26.734624784023165	28.448624752891366	24.487298227044192
27	21.122145603412047	26.347812213003753	28.025232321031083	24.504809862553117
28	18.548908052239156	25.164220226328162	32.3688962221565	23.917975499276185
29	22.65188425198076	24.35284778108121	28.995766075681395	23.999501891256635
30	21.866195538813567	26.24955248042588	28.532680603334214	23.351571377426332
31	25.327856731472693	25.476316485842815	26.247023021963482	22.948803760721013
32	27.513892564170416	26.077354731254765	25.261701663994522	21.147051040580298
33	23.169450368133493	25.33680712295503	27.99234936101988	23.5013931478916
34	23.70861416808057	23.77457466183086	29.586297339787993	22.930513830300576
35	24.42970440359261	23.258954282956896	26.79299690238625	25.51834441106424
36	25.467949815544106	23.46208925486045	29.407289510141183	21.66267141945426
37	24.16547328113569	24.04094609529443	30.05230141805333	21.741279205516552
38	23.144739504693117	27.02979312921252	26.47681459458618	23.34865277150818
39	24.376002054698567	24.71514406238812	29.412932148249617	21.495921734663696
40	26.38750525349065	23.439518702426724	27.777539965443705	22.395436078638916
41	23.395155892470775	23.231908534782	27.016756689444765	26.356178883302462
42	24.303815201656214	21.90180253101505	29.554970969599804	24.239411297728935
43	24.23260121725324	21.7383605995984	29.600501221923007	24.428536961225348
44	22.273632924987936	23.188324019737557	29.14247466650063	25.395568388773874
45	24.055149977429448	22.472487274878198	26.487127002163657	26.985235745528698
46	25.96489111654188	23.276465918465824	27.728312812290834	23.03033015270146
47	21.85841258969849	22.42034151580717	32.3292031816696	23.392042712824743
48	22.944133991251967	21.086733184938435	31.044432856497984	24.924699967311614
49	22.88420528306586	20.403001105178774	33.65561228460688	23.05718132714848
50	22.841593636660804	20.669177964914464	31.282591099419392	25.20663729900534
51	21.950835110440046	21.12389676696294	27.56156312750027	29.36370499509674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	631.5
2	749.0
3	1320.0
4	1891.0
5	1334.5
6	778.0
7	780.0
8	782.0
9	833.5
10	885.0
11	939.5
12	994.0
13	1026.5
14	1059.0
15	1009.0
16	959.0
17	947.0
18	935.0
19	951.0
20	967.0
21	961.5
22	956.0
23	1397.0
24	1838.0
25	2085.5
26	2844.5
27	3356.0
28	3591.5
29	3827.0
30	4642.0
31	5457.0
32	6734.5
33	8012.0
34	9631.0
35	11250.0
36	12127.0
37	13004.0
38	14270.0
39	15536.0
40	17751.5
41	19967.0
42	23287.0
43	26607.0
44	29523.0
45	32439.0
46	36588.5
47	40738.0
48	47840.5
49	54943.0
50	61083.0
51	67223.0
52	65251.5
53	63280.0
54	51516.5
55	39753.0
56	33747.5
57	27742.0
58	24531.0
59	21320.0
60	18042.5
61	14765.0
62	12513.0
63	10261.0
64	8764.0
65	7267.0
66	5749.0
67	4231.0
68	3539.0
69	2847.0
70	2287.0
71	1727.0
72	1474.0
73	1221.0
74	1088.5
75	649.0
76	342.0
77	241.0
78	140.0
79	99.0
80	58.0
81	39.0
82	20.0
83	12.0
84	4.0
85	3.5
86	3.0
87	3.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.486518672543433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.9682310453561	22.030560132944387
2	12.894659177884375	8.120158539610799
3	5.145534280322775	4.860448835927862
4	2.555368479650072	3.218386293989237
5	1.5101773297342647	2.377511334576486
6	0.9730488223299838	1.838275194815366
7	0.6805764144718808	1.5000287387653073
8	0.5455429913352899	1.3741799666683046
9	0.39422656962363267	1.1171540021100528
>10	4.753691773551281	36.706911476580395
>50	0.5091239352934931	9.925900441800072
>100	0.061169016497640546	3.361160736421863
>500	0.006178688535115207	1.4468340798037502
>1k	0.002471475414046083	2.122490225986139
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3739	0.7275111685319802	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3559	0.6924878975141261	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	2106	0.4097722709088928	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1506	0.2930280341827125	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	994	0.19340628550970534	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	934	0.1817318618370873	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	897	0.17453263390563953	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	874	0.17005743816446928	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	827	0.16091247295425182	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	679	0.1321155612284607	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	645	0.1255000544806438	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	577	0.11226904098501005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.837211836309014E-4	0.0	0.0	0.4329265445262519	0.0
2	5.837211836309014E-4	0.0	0.0	2.451434397521909	0.0
3	7.782949115078685E-4	0.0	0.0	3.2593045156670764	0.0
4	7.782949115078685E-4	0.0	0.0	4.2111591924412	0.0
5	7.782949115078685E-4	0.0	0.0	6.655783509487415	0.0
6	7.782949115078685E-4	0.0	0.0	7.80065532431549	0.0
7	7.782949115078685E-4	0.0	0.0	9.033085316688199	0.0
8	7.782949115078685E-4	0.0	0.0	10.891653565368989	0.0
9	7.782949115078685E-4	0.0	0.0	11.525769344520025	0.0
10	7.782949115078685E-4	0.0	0.0	13.16855532898526	0.0
11	7.782949115078685E-4	0.0	0.0	15.346419065112153	0.0
12	7.782949115078685E-4	0.0	0.0	17.332627679280233	0.0
13	7.782949115078685E-4	0.0	0.0	18.05002101396261	0.0
14	7.782949115078685E-4	0.0	0.0	18.351804865899787	0.0
15	7.782949115078685E-4	0.0	0.0	18.901281073424343	0.0
16	7.782949115078685E-4	0.0	0.0	20.021247451084164	0.0
17	7.782949115078685E-4	0.0	0.0	21.445721712871443	0.0
18	7.782949115078685E-4	0.0	0.0	23.10290615319957	0.0
19	7.782949115078685E-4	0.0	0.0	23.990551499774295	0.0
20	7.782949115078685E-4	0.0	0.0	24.82741310337313	0.0
21	7.782949115078685E-4	0.0	0.0	25.87441433307909	0.0
22	7.782949115078685E-4	0.0	0.0	26.871020967264915	0.0
23	7.782949115078685E-4	0.0	0.0	27.865487290444094	0.0
24	7.782949115078685E-4	0.0	0.0	28.67744345687468	0.0
25	7.782949115078685E-4	0.0	0.0	29.414294164344753	0.0
26	7.782949115078685E-4	0.0	0.0	30.111062683872174	0.0
27	7.782949115078685E-4	0.0	0.0	30.735255202901484	0.0
28	7.782949115078685E-4	0.0	0.0	31.376764783711845	0.0
29	7.782949115078685E-4	0.0	0.0	32.02333328144701	0.0
30	7.782949115078685E-4	0.0	0.0	32.805519667512414	0.0
31	9.728686393848357E-4	0.0	0.0	33.531863393677135	0.0
32	9.728686393848357E-4	0.0	0.0	34.202753607396915	0.0
33	0.0011674423672618029	0.0	0.0	34.87403296857245	0.0
34	0.0011674423672618029	0.0	0.0	35.52974643151783	0.0
35	0.0011674423672618029	0.0	0.0	36.22846068832402	0.0
36	0.0011674423672618029	0.0	0.0	36.878142365705216	0.0
37	0.0011674423672618029	0.0	0.0	37.508950391482344	0.0
38	0.0011674423672618029	0.0	0.0	38.14150958081036	0.0
39	0.00136201609513877	0.0	0.0	38.81492925299254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	30	2.1631495E-6	45.000004	2
TATTGCG	30	2.1631495E-6	45.000004	1
CTAATGG	70	0.0	45.000004	2
TGATATC	25	3.887785E-5	45.0	24
AGGTACG	25	3.887785E-5	45.0	1
TCTTGCG	25	3.887785E-5	45.0	1
GTCGATA	25	3.887785E-5	45.0	40
AACCCAC	20	7.0296414E-4	45.0	41
CGCATGT	20	7.0296414E-4	45.0	44
ATTACGG	25	3.887785E-5	45.0	2
TAAACCG	20	7.0296414E-4	45.0	9
TTACTAG	25	3.887785E-5	45.0	1
CGATTCA	100	0.0	45.0	10
TAACGCG	20	7.0296414E-4	45.0	1
CCATGCG	20	7.0296414E-4	45.0	1
AAACGAT	20	7.0296414E-4	45.0	11
TACGATG	20	7.0296414E-4	45.0	1
ACTACGG	40	6.8030204E-9	45.0	2
TCGTCAA	20	7.0296414E-4	45.0	40
ACAGACG	25	3.887785E-5	45.0	1
>>END_MODULE