FastQCFastQC Report
Sat 14 Jan 2017
SRR2935778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580450
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG44330.2804897339365371No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.2545477553861242No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC30360.19209718750988644No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC23210.14685690784270303No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC23160.14654054225062482No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT17730.11218323895093171Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.0340896E-445.0000041
ATCGTAC207.0340896E-445.00000430
CTAGTCG207.0340896E-445.0000041
TCTAACG406.8175723E-945.0000041
ATAACGG750.045.02
TTAACGG900.040.02
AATGCGG2050.039.5121962
CGTTTTT22500.038.31
CGGTCTA2000.038.25000431
TAACGGG4000.037.6875043
CGTAAGG2150.037.674422
CTAACGG1200.037.5000042
TATTCGC301.1401155E-437.50000412
CCGCAAT301.1401155E-437.50000425
CACGACG2050.037.31707426
ACGGGAT3650.036.369865
CTATCGA250.00210734736.00000423
TACGGGA4000.036.0000044
CGTAGAA250.00210734736.00000421
TACCGCG250.00210734736.0000041
Produced by FastQC (version 0.11.5)