SRR2935776.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867436
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	7848	0.9047353349411369	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	6979	0.8045550334549176	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	6575	0.757980992257642	Illumina PCR Primer Index 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5574	0.6425834297861744	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3382	0.38988467160689666	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2225	0.2565030734255899	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1437	0.16566063663486413	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1433	0.16519950751409904	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1365	0.15736031246109222	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1186	0.13672478430685375	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1180	0.1360330906257061	Illumina PCR Primer Index 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTAACT	1151	0.13268990450015908	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTA	1008	0.11620453843280656	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	1007	0.1160892561526153	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	963	0.11101683582419913	Illumina PCR Primer Index 2 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAC	20	7.0323277E-4	45.000004	43
CGACGTG	20	7.0323277E-4	45.000004	16
GACACGA	30	2.164883E-6	45.000004	25
TACGCAC	20	7.0323277E-4	45.000004	29
CGCTACA	20	7.0323277E-4	45.000004	30
GTATCGC	20	7.0323277E-4	45.000004	9
CGCCCGA	20	7.0323277E-4	45.000004	40
CTATGCG	25	3.8900107E-5	45.0	1
CTCGTAG	25	3.8900107E-5	45.0	1
TTTCGCG	35	1.2114651E-7	45.0	1
TCGATAG	35	1.2114651E-7	45.0	1
CTAACGG	45	3.8380676E-10	45.0	2
CCAACGG	45	3.8380676E-10	45.0	2
GATTACG	25	3.8900107E-5	45.0	1
TATCGCG	25	3.8900107E-5	45.0	1
TCGAACG	25	3.8900107E-5	45.0	1
ACAACGA	350	0.0	42.42857	13
CAACGAG	350	0.0	42.42857	14
CACAACG	360	0.0	41.25	12
TACGGCT	995	0.0	40.25126	7