##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283978
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91788800540887	33.0	31.0	34.0	30.0	34.0
2	31.957229081126002	33.0	31.0	34.0	30.0	34.0
3	31.99599264731775	33.0	31.0	34.0	30.0	34.0
4	35.670129376219286	37.0	35.0	37.0	33.0	37.0
5	34.8147708625316	37.0	35.0	37.0	33.0	37.0
6	35.279113170738576	37.0	35.0	37.0	33.0	37.0
7	35.87865961447718	37.0	35.0	37.0	35.0	37.0
8	35.91316580861898	37.0	35.0	37.0	35.0	37.0
9	37.762235102719224	39.0	38.0	39.0	35.0	39.0
10	37.36143644930241	39.0	37.0	39.0	34.0	39.0
11	37.22325673115523	39.0	37.0	39.0	34.0	39.0
12	37.035104832064455	39.0	35.0	39.0	34.0	39.0
13	36.82525054757763	39.0	35.0	39.0	33.0	39.0
14	37.716805527188725	40.0	36.0	41.0	33.0	41.0
15	37.94254132362366	40.0	36.0	41.0	33.0	41.0
16	38.01288480093528	40.0	36.0	41.0	34.0	41.0
17	38.07508328109924	40.0	36.0	41.0	34.0	41.0
18	38.0982716971033	40.0	36.0	41.0	34.0	41.0
19	37.99470733648381	40.0	36.0	41.0	34.0	41.0
20	37.989199867595374	40.0	36.0	41.0	34.0	41.0
21	37.88994922141856	39.0	35.0	41.0	34.0	41.0
22	37.954795794040386	40.0	35.0	41.0	34.0	41.0
23	37.98758706660375	40.0	35.0	41.0	34.0	41.0
24	37.93947770601948	40.0	35.0	41.0	34.0	41.0
25	37.817813351738515	39.0	35.0	41.0	34.0	41.0
26	37.87242321588292	40.0	35.0	41.0	34.0	41.0
27	37.87460648360084	40.0	35.0	41.0	34.0	41.0
28	37.79523765925529	40.0	36.0	41.0	34.0	41.0
29	37.790663361246295	40.0	36.0	41.0	34.0	41.0
30	37.699476720027604	40.0	36.0	41.0	34.0	41.0
31	37.594317165414225	40.0	35.0	41.0	33.0	41.0
32	37.52936495080605	39.0	35.0	41.0	33.0	41.0
33	37.30322771482298	39.0	35.0	41.0	33.0	41.0
34	37.29475170611808	39.0	35.0	41.0	33.0	41.0
35	37.26805597616717	39.0	35.0	41.0	33.0	41.0
36	37.083583939600956	39.0	35.0	41.0	32.0	41.0
37	37.03267506637838	39.0	35.0	41.0	32.0	41.0
38	36.94157646014832	39.0	35.0	41.0	32.0	41.0
39	36.905161667453115	39.0	35.0	41.0	32.0	41.0
40	36.81779574474079	39.0	35.0	41.0	31.0	41.0
41	36.717154145743685	39.0	35.0	41.0	31.0	41.0
42	36.69295156667066	39.0	35.0	41.0	31.0	41.0
43	36.55160611033249	39.0	35.0	41.0	31.0	41.0
44	36.39887949066477	39.0	35.0	40.0	31.0	41.0
45	36.41029234659023	39.0	35.0	40.0	31.0	41.0
46	36.30442851206784	39.0	35.0	40.0	31.0	41.0
47	36.16145969053941	38.0	35.0	40.0	30.0	41.0
48	36.12508011183965	38.0	35.0	40.0	30.0	41.0
49	36.0675580502715	38.0	35.0	40.0	30.0	41.0
50	35.93057208657009	38.0	35.0	40.0	30.0	41.0
51	34.63308777440506	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	4.0
10	8.0
11	4.0
12	4.0
13	5.0
14	2.0
15	12.0
16	26.0
17	34.0
18	64.0
19	123.0
20	175.0
21	308.0
22	465.0
23	717.0
24	1129.0
25	1581.0
26	1993.0
27	2300.0
28	2725.0
29	3172.0
30	3959.0
31	5432.0
32	7199.0
33	10640.0
34	20176.0
35	29507.0
36	19957.0
37	30882.0
38	57278.0
39	84041.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.86956031805281	21.4414496897647	27.568684898125912	12.120305094056581
2	29.979435026656994	25.23188416004057	27.132383494496054	17.656297318806384
3	29.23641972265457	23.446182450753227	30.465388163871847	16.852009662720352
4	28.823359556021945	25.44246385283367	28.713844030171355	17.020332560973035
5	23.89304805301819	30.656600159167258	27.12639711526949	18.323954672545057
6	24.432174323363075	32.59196134911859	26.50487009557078	16.470994231947543
7	77.42747677637	5.945883131791899	10.8307685806647	5.7958715111734005
8	78.35043559712372	4.29822028466994	9.782447936107728	7.568896182098614
9	72.47885399573207	7.396699744346392	13.244687968786314	6.879758291135228
10	38.55016937931811	29.132538436076032	19.287761728021184	13.029530456584665
11	27.981040784849533	24.735366824190606	27.919416292811416	19.364176098148448
12	26.88306840670756	21.040010141630688	33.51597658973582	18.560944861925925
13	22.201015571628787	29.433970237131046	31.652099810548705	16.712914380691462
14	16.199846466979835	33.51280733014529	29.697018783145175	20.590327419729697
15	14.409566938283952	27.456000112684787	40.11261435745023	18.021818591581038
16	16.01708583059251	25.372740141841977	35.67212953116086	22.93804449640465
17	15.853340751748373	25.15194839036827	28.299727443675216	30.694983414208142
18	18.9990069653283	25.138567072097135	34.70374465627619	21.158681306298373
19	20.937185274915663	26.84574157153019	28.409947249434815	23.807125904119335
20	21.51751191993746	27.001387431420742	30.728084569931475	20.753016078710324
21	19.966687560303967	27.965546626851374	31.814436329574825	20.25332948326983
22	18.040482009169725	26.4862066779821	27.935262590764072	27.538048722084106
23	15.232165872004169	28.419807168160915	28.80786539802379	27.54016156181113
24	19.53073829662861	24.91566248089641	32.329969222967975	23.223629999507004
25	16.898492136714815	27.232743381529556	30.066061455464858	25.802703026290768
26	15.267027727500018	28.56946664882491	32.1926346407116	23.97087098296347
27	17.21893949531302	28.653980237905753	33.512103050236284	20.614977216544943
28	14.704308080203395	29.167400291571884	33.79346287388459	22.334828754340126
29	17.13407376627767	24.530773510624062	34.55936727492975	23.77578544816852
30	19.542006775172723	25.568529956545934	31.798942171576673	23.090521096704673
31	23.675073421180514	28.93111438210002	25.719597997027936	21.674214199691523
32	25.273084534717476	26.65769883582531	27.08836600018311	20.980850629274098
33	21.558008014705365	25.18293670636458	29.84315686426413	23.415898414665925
34	20.470247695243998	24.361042052553366	31.44363295748262	23.725077294720013
35	20.868165843832973	22.385889047743134	31.12987625801999	25.616068850403906
36	22.58449598208312	23.38526223862412	32.749015768827164	21.2812260104656
37	21.303058687644818	25.734035735162582	33.39941826479516	19.56348731239744
38	21.33404700364113	28.046890956341684	26.981667593968545	23.63739444604864
39	22.948608695039756	22.662318912028397	31.050996908211197	23.338075484720648
40	26.365774813541893	20.729422701758587	29.81287282817683	23.091929656522687
41	21.43370261076562	22.033749093239617	29.25649170006127	27.27605659593349
42	22.337998013930658	22.050651811055786	32.191930360802594	23.419419814210958
43	23.419067674256453	22.805992013465833	31.327778912450967	22.447161399826747
44	20.133601898738636	24.669516652698448	29.468832092626897	25.728049355936022
45	21.517864059891963	23.644437245138707	27.39437562064667	27.443323074322656
46	25.330835487256053	22.289050560254665	29.813577108085838	22.56653684440344
47	20.824500489474538	22.740141841973674	33.94840445386615	22.486953214685645
48	21.001626886589804	21.05937783912838	32.25778053229475	25.681214741987056
49	21.556247314932847	19.416292811414966	36.30915070885773	22.718309164794455
50	22.01085999619689	19.569121551669497	32.43807618900056	25.981942263133064
51	19.654339420659348	20.893167780602724	28.211692455049338	31.240800343688598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	677.0
1	640.0
2	603.0
3	966.5
4	1330.0
5	924.5
6	519.0
7	542.5
8	566.0
9	610.5
10	655.0
11	677.5
12	700.0
13	724.5
14	749.0
15	750.5
16	752.0
17	799.0
18	846.0
19	808.0
20	770.0
21	816.5
22	863.0
23	927.5
24	992.0
25	999.0
26	1378.0
27	1750.0
28	2256.0
29	2762.0
30	3138.0
31	3514.0
32	3806.5
33	4099.0
34	5204.0
35	6309.0
36	6742.5
37	7176.0
38	8305.0
39	9434.0
40	11529.0
41	13624.0
42	17217.0
43	20810.0
44	23035.0
45	25260.0
46	30173.5
47	35087.0
48	39445.5
49	43804.0
50	40958.0
51	38112.0
52	29931.5
53	21751.0
54	17375.0
55	12999.0
56	10859.0
57	8719.0
58	7512.5
59	6306.0
60	5306.0
61	4306.0
62	3637.0
63	2968.0
64	2276.0
65	1584.0
66	1296.0
67	1008.0
68	831.0
69	654.0
70	485.0
71	316.0
72	267.0
73	218.0
74	220.5
75	140.0
76	57.0
77	48.5
78	40.0
79	32.5
80	25.0
81	20.0
82	15.0
83	15.5
84	16.0
85	9.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	283978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.80684419215573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57626765551436	23.52154040101698
2	13.001541602433232	8.790821824225821
3	5.4685221449106285	5.546204283430407
4	2.8436315153535268	3.8453683031784154
5	1.6749302112411983	2.8312052342082836
6	1.1072455314361902	2.2459486298234372
7	0.7208032998625057	1.7057659396150406
8	0.5458105912253656	1.476170689278747
9	0.42914878546727214	1.3057349512990444
>10	4.14357735094371	32.41765207163935
>50	0.42185742260739134	8.760185648184015
>100	0.0583309028790467	3.6576777074280398
>500	0.00416649306278905	0.945143637887442
>1k	0.00416649306278905	2.950580678784976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2970	1.0458556648754482	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2561	0.9018304234835094	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	1681	0.5919472635204135	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1167	0.4109473269056053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	798	0.28100768369380724	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	713	0.25107578756100823	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	592	0.20846685306608256	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	581	0.20459331356654387	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC	489	0.17219643775222024	No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	383	0.13486960257484734	No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	374	0.13170034298431568	No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	358	0.1260661037122594	No Hit
TGCGAAGGGCTGCACCCACATGGTGGAGCACAGCCATCTCTAACTCCCTTC	348	0.12254470416722422	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	341	0.1200797244856996	No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	339	0.11937544457669255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.042799090070358E-4	0.0	0.0	0.6261048391072548	0.0
2	7.042799090070358E-4	0.0	0.0	3.407306199776039	0.0
3	7.042799090070358E-4	0.0	0.0	4.531689074505771	0.0
4	7.042799090070358E-4	0.0	0.0	5.721217840818655	0.0
5	7.042799090070358E-4	0.0	0.0	8.658417201332497	0.0
6	7.042799090070358E-4	0.0	0.0	10.12719295156667	0.0
7	7.042799090070358E-4	0.0	0.0	11.676256611427645	0.0
8	7.042799090070358E-4	0.0	0.0	13.951784997429378	0.0
9	7.042799090070358E-4	0.0	0.0	14.824387804689096	0.0
10	7.042799090070358E-4	0.0	0.0	16.74355055673327	0.0
11	7.042799090070358E-4	0.0	0.0	19.56736085189698	0.0
12	7.042799090070358E-4	0.0	0.0	21.760488488544887	0.0
13	7.042799090070358E-4	0.0	0.0	22.720774144475982	0.0
14	7.042799090070358E-4	0.0	0.0	23.080661177978577	0.0
15	7.042799090070358E-4	0.0	0.0	23.64056370563917	0.0
16	7.042799090070358E-4	0.0	0.0	24.93996013775715	0.0
17	7.042799090070358E-4	0.0	0.0	26.71192838881885	0.0
18	7.042799090070358E-4	0.0	0.0	28.90787314510279	0.0
19	7.042799090070358E-4	0.0	0.0	30.01394474219834	0.0
20	7.042799090070358E-4	0.0	0.0	30.96296191958532	0.0
21	7.042799090070358E-4	0.0	0.0	32.09016191395108	0.0
22	7.042799090070358E-4	0.0	0.0	33.293776278444106	0.0
23	7.042799090070358E-4	0.0	0.0	34.56042369479326	0.0
24	7.042799090070358E-4	0.0	0.0	35.50380663290818	0.0
25	7.042799090070358E-4	0.0	0.0	36.24928691659213	0.0
26	7.042799090070358E-4	0.0	0.0	36.92257850960286	0.0
27	7.042799090070358E-4	0.0	0.0	37.65995957433322	0.0
28	7.042799090070358E-4	0.0	0.0	38.3786772214749	0.0
29	7.042799090070358E-4	0.0	0.0	39.156554380973176	0.0
30	7.042799090070358E-4	0.0	0.0	40.026340068596866	0.0
31	7.042799090070358E-4	0.0	0.0	40.824289205501834	0.0
32	7.042799090070358E-4	0.0	0.0	41.53490763368993	0.0
33	7.042799090070358E-4	0.0	0.0	42.19024008902098	0.0
34	7.042799090070358E-4	0.0	0.0	42.89487213798252	0.0
35	7.042799090070358E-4	0.0	0.0	43.700216213932066	0.0
36	7.042799090070358E-4	0.0	0.0	44.37491636676081	0.0
37	7.042799090070358E-4	0.0	0.0	45.012641824366675	0.0
38	7.042799090070358E-4	0.0	0.0	45.623252505475776	0.0
39	7.042799090070358E-4	0.0	0.0	46.35042151152554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTGAC	20	7.024305E-4	45.000004	45
CGGGTGA	30	2.1597098E-6	45.000004	6
ATGGGCG	25	3.8833627E-5	45.000004	5
CGGGTCC	20	7.024305E-4	45.000004	6
TTGGCGG	50	2.1827873E-11	45.000004	2
GTCGCAC	20	7.024305E-4	45.000004	38
CGAACGG	30	2.1597098E-6	45.000004	2
ACGCCGT	20	7.024305E-4	45.000004	36
GATCGAC	20	7.024305E-4	45.000004	9
CGAGTGG	25	3.8833627E-5	45.000004	3
GGTCGCA	20	7.024305E-4	45.000004	37
CACGGGT	50	2.1827873E-11	45.000004	4
CCTAGTT	40	6.7830115E-9	45.000004	36
CGACGAT	20	7.024305E-4	45.000004	25
CGTGCGA	25	3.8833627E-5	45.000004	39
GGCACGG	30	2.1597098E-6	45.000004	2
TAGGTTG	20	7.024305E-4	45.000004	1
TTTCGAG	20	7.024305E-4	45.000004	1
ATTACGG	25	3.8833627E-5	45.000004	2
GGCCGAT	40	6.7830115E-9	45.000004	8
>>END_MODULE