SRR2935771.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935771.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894629
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2499	0.27933366792268083	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2469	0.27598032256946736	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2118	0.23674618193686991	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1803	0.20153605572812866	Illumina Single End Adapter 2 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1195	0.13357492323633594	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	1037	0.1159139710427451	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1024	0.11446085472301927	TruSeq Adapter, Index 22 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	954	0.10663638223218787	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	930	0.10395370594961711	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.1649594E-6	45.000004	21
ATATGCG	30	2.1649594E-6	45.000004	1
TCGTTGA	40	6.8121153E-9	45.0	24
GCAACGC	20	7.032448E-4	45.0	27
TGCGATG	20	7.032448E-4	45.0	1
ACGTGCG	20	7.032448E-4	45.0	1
CGTCGGA	20	7.032448E-4	45.0	19
GTACGTC	25	3.8901107E-5	45.0	17
CGTTTTT	1640	0.0	41.844513	1
GTTAGCG	65	0.0	41.538464	1
CTACGAA	180	0.0	41.25	11
CGATACG	55	6.002665E-11	40.909092	10
CGTCATA	45	1.9274012E-8	40.0	38
TGTAGCG	85	0.0	39.705883	1
CACAACG	240	0.0	39.375004	12
ATCTACG	35	6.246735E-6	38.57143	12
ACGTTAG	35	6.246735E-6	38.57143	1
TATCCGG	35	6.246735E-6	38.57143	2
GCTACGA	195	0.0	38.076923	10
AACGAGC	250	0.0	37.8	15