FastQCFastQC Report
Sat 14 Jan 2017
SRR2935770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596810
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC66281.1105712035656239No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG61401.0288031366766643No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC55620.9319548935171997TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC53260.8924113201856537No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC45070.7551817161240596TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT27020.45274040314337893TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC18270.3061275782912485TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT12890.2159816356964528No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC12220.20475528225063255TruSeq Adapter, Index 21 (95% over 21bp)
TTGTGCGGGATATTGTGTGTAGGAGGAAACTTGAGTTTGGGGAATTATGTT11690.19587473400244632No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.19336137129069553No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT10820.18129723027429165No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG9320.15616360315678357No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA9260.15515825807208325No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT9090.152309780332099No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC8940.14979641762034818No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC8160.13672693151924398No Hit
GCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG7450.12483034801695683TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG6880.11527956971230376No Hit
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG6520.1092474992041018No Hit
GGTTCGGGATGTGTGCTGTACTAGGCTTCATGATGCATTTCTAAATTTGTG6440.10790703909116806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCT302.1637406E-645.00000415
GTAAGCG302.1637406E-645.0000041
ATCTCGA253.888543E-545.012
TTAGACG207.0305576E-445.01
CGCATCG253.888543E-545.021
GACGGTC207.0305576E-445.029
GAACGTA207.0305576E-445.01
ACGCTAG207.0305576E-445.01
CGGTCTA207.0305576E-445.031
CGCACCA207.0305576E-445.039
AACTACG207.0305576E-445.013
ACCCCTA253.888543E-545.028
GTAACGT253.888543E-545.013
GCGTAAG453.8380676E-1045.01
GTTATCG207.0305576E-445.018
TGATTAG750.042.01
CTATAGG1350.041.6666682
TAGGGCA1800.041.255
AGGGCTC1650.040.9090926
CTCCCAT501.0804797E-940.538
Produced by FastQC (version 0.11.5)