##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935770.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596810
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90314170338969	33.0	31.0	34.0	30.0	34.0
2	31.95347095390493	33.0	31.0	34.0	30.0	34.0
3	31.98914729981066	33.0	31.0	34.0	30.0	34.0
4	35.6754528241819	37.0	35.0	37.0	33.0	37.0
5	34.71834922337092	37.0	35.0	37.0	33.0	37.0
6	35.22638360617282	37.0	35.0	37.0	33.0	37.0
7	35.82360550258876	37.0	35.0	37.0	35.0	37.0
8	35.80198388096714	37.0	35.0	37.0	35.0	37.0
9	37.67179671922387	39.0	37.0	39.0	35.0	39.0
10	37.29571052763861	39.0	37.0	39.0	34.0	39.0
11	37.22295202828371	39.0	37.0	39.0	34.0	39.0
12	37.13636500728875	39.0	37.0	39.0	34.0	39.0
13	37.025212379149146	39.0	37.0	39.0	33.0	39.0
14	38.062571002496604	40.0	37.0	41.0	33.0	41.0
15	38.12281295554699	40.0	37.0	41.0	33.0	41.0
16	38.20852365074312	40.0	37.0	41.0	34.0	41.0
17	38.18446574286624	40.0	37.0	41.0	34.0	41.0
18	38.19642097149847	40.0	37.0	41.0	34.0	41.0
19	38.13418843518038	40.0	37.0	41.0	34.0	41.0
20	38.12693151924398	40.0	37.0	41.0	34.0	41.0
21	38.10745463380305	40.0	37.0	41.0	34.0	41.0
22	38.145386303848795	40.0	37.0	41.0	34.0	41.0
23	38.13639516764129	40.0	37.0	41.0	34.0	41.0
24	38.07589852716945	40.0	37.0	41.0	34.0	41.0
25	37.96752232703876	40.0	36.0	41.0	34.0	41.0
26	37.99642432264875	40.0	37.0	41.0	34.0	41.0
27	37.971466630921064	40.0	37.0	41.0	34.0	41.0
28	37.91678256061393	40.0	36.0	41.0	34.0	41.0
29	37.886590372145236	40.0	36.0	41.0	34.0	41.0
30	37.71390727367169	40.0	36.0	41.0	33.0	41.0
31	37.69674435750071	40.0	36.0	41.0	33.0	41.0
32	37.54408940868953	40.0	36.0	41.0	33.0	41.0
33	37.409195556374726	40.0	36.0	41.0	33.0	41.0
34	37.374402238568386	40.0	36.0	41.0	33.0	41.0
35	37.222417519813675	39.0	35.0	41.0	32.0	41.0
36	37.18280189675106	39.0	35.0	41.0	32.0	41.0
37	37.09568706958664	39.0	35.0	41.0	32.0	41.0
38	37.04350128181498	39.0	35.0	41.0	32.0	41.0
39	37.066461687974396	39.0	35.0	41.0	32.0	41.0
40	36.9174896533235	39.0	35.0	41.0	31.0	41.0
41	36.84203850471675	39.0	35.0	41.0	31.0	41.0
42	36.864352138871666	39.0	35.0	41.0	31.0	41.0
43	36.78330624486855	39.0	35.0	41.0	31.0	41.0
44	36.697310701898424	39.0	35.0	41.0	31.0	41.0
45	36.671007523332385	39.0	35.0	41.0	31.0	41.0
46	36.560290544729476	39.0	35.0	41.0	31.0	41.0
47	36.41329568874516	39.0	35.0	40.0	31.0	41.0
48	36.36576967544109	39.0	35.0	40.0	31.0	41.0
49	36.37243008662723	39.0	35.0	40.0	31.0	41.0
50	36.239468172450195	39.0	35.0	40.0	30.0	41.0
51	34.96716542953369	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	8.0
11	5.0
12	11.0
13	8.0
14	7.0
15	25.0
16	29.0
17	67.0
18	108.0
19	249.0
20	488.0
21	738.0
22	1066.0
23	1516.0
24	2151.0
25	2915.0
26	3930.0
27	4650.0
28	5536.0
29	6937.0
30	8617.0
31	11414.0
32	15443.0
33	21725.0
34	37093.0
35	49420.0
36	45091.0
37	69421.0
38	123031.0
39	184990.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75548331964947	21.27729093011176	25.131783984852802	10.83544176538597
2	29.385063923191634	28.225901040532165	24.813424708031032	17.57561032824517
3	28.854744391012215	27.36716878068397	27.811531308121513	15.966555520182302
4	28.517618672609373	23.81360902129656	29.861262378311356	17.80750992778271
5	26.107136274526233	29.237780868283036	25.543305239523466	19.111777617667265
6	23.802047552822504	35.195623397731275	24.976625726780718	16.025703322665503
7	74.84660109582613	5.531743771049412	13.894204185586704	5.727450947537743
8	74.93004473785628	7.568740470166385	10.214976290611752	7.2862385013655935
9	69.56250733064124	6.914093262512358	12.498450092994421	11.02494931385198
10	39.05782409812168	25.45718067726747	20.40314337896483	15.08185184564602
11	28.329786699284533	24.69194551029641	26.581994269533016	20.396273520886044
12	24.385147701948696	21.238082471808447	32.8870159682311	21.489753858011763
13	21.721988572577537	24.464737521154138	35.2810777299308	18.53219617633753
14	18.40636048323587	29.264925185569947	30.73490725691594	21.593807074278246
15	16.870528308842008	25.80687320922907	38.244835039627354	19.07776344230157
16	18.28287059533185	26.15170657328128	31.26455655903889	24.300866272347985
17	18.576766475092576	26.080327072267558	30.33511502823344	25.007791424406427
18	19.163888004557563	25.408756555687738	33.07451282652771	22.35284261322699
19	19.410197550309142	27.100417218210147	30.137229604061595	23.352155627419112
20	21.126656724920828	27.336170640572377	32.146746870863424	19.39042576364337
21	21.033997419614284	28.234949146294465	31.366431527621856	19.364621906469395
22	18.89378529180141	24.723446322950352	30.574722273420353	25.80804611182789
23	18.656021179269786	27.26093731673397	30.158676965868537	23.924364538127712
24	22.1113922353848	23.786632261523767	29.494646537423968	24.607328965667467
25	19.21013387845378	28.77347899666561	27.277022838089177	24.73936428679144
26	19.751512206564904	26.30183810592986	29.093848963656775	24.85280072384846
27	20.509709957943066	28.740302608870493	28.604581022435948	22.14540641075049
28	16.122886680853203	26.503577352926396	33.658953435766826	23.71458253045358
29	21.119451751813813	26.771501817999027	29.281345821953387	22.827700608233776
30	20.551599336472247	31.06985472763526	28.693218947403697	19.6853269884888
31	22.94566109817195	27.924800187664417	27.391967292773245	21.737571421390392
32	23.331881168210987	30.989092005831004	26.4305222767715	19.248504549186507
33	22.383170523282118	29.27062214104992	25.755768167423472	22.5904391682445
34	24.54717581809956	24.847606440910845	29.67778690035355	20.92743084063605
35	21.027630234077847	27.267136944756288	28.496003753288317	23.209229067877548
36	27.31556106633602	25.819942695330173	27.885424171846985	18.97907206648682
37	20.94988354752769	28.129387912400933	32.59596856621035	18.324759973861028
38	19.39712806420804	29.79993632814464	28.833464586719394	21.969471020927934
39	24.731153968599724	27.133593606005263	27.83163820981552	20.303614215579497
40	23.843099143781103	24.192289003200347	30.866775020525793	21.097836832492753
41	23.97697759756036	24.010656657897826	26.989494143864885	25.022871600676933
42	23.17705802516714	23.20302943985523	31.744273721955064	21.87563881302257
43	25.220086794792312	23.60416212865066	28.345537105611502	22.830213970945525
44	21.60184983495585	24.89050116452472	29.144786447948256	24.362862552571173
45	21.025619543908448	23.997754729310834	28.222214775221595	26.75441095155912
46	25.324810241115266	24.84827667096731	28.649486436219235	21.177426651698195
47	19.830599353227996	24.495903218779848	34.48132571505169	21.192171712940468
48	20.841641393408285	24.00831085270019	30.502840099864276	24.647207654027245
49	22.48269968666745	21.053936763794173	34.61704730148624	21.846316248052144
50	22.839932306764297	20.617952112062465	31.7015465558553	24.84056902531794
51	21.0802432935105	20.993113386169803	27.500712119434993	30.425931200884705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	533.0
1	660.0
2	787.0
3	1466.0
4	2145.0
5	1436.5
6	728.0
7	763.5
8	799.0
9	853.5
10	908.0
11	986.0
12	1064.0
13	1128.5
14	1193.0
15	1151.5
16	1110.0
17	1095.5
18	1081.0
19	1148.5
20	1216.0
21	1388.0
22	1560.0
23	1956.0
24	2352.0
25	3222.5
26	4379.0
27	4665.0
28	5871.5
29	7078.0
30	7245.0
31	7412.0
32	8848.5
33	10285.0
34	11911.5
35	13538.0
36	14930.0
37	16322.0
38	18655.5
39	20989.0
40	26389.5
41	31790.0
42	37999.0
43	44208.0
44	64224.5
45	84241.0
46	82678.0
47	81115.0
48	77995.0
49	74875.0
50	60805.5
51	46736.0
52	39942.0
53	33148.0
54	28320.0
55	23492.0
56	21358.0
57	19224.0
58	17811.5
59	16399.0
60	14492.5
61	12586.0
62	11073.5
63	9561.0
64	8122.5
65	6684.0
66	5684.5
67	4685.0
68	3915.0
69	3145.0
70	2685.5
71	2226.0
72	1724.5
73	1223.0
74	1058.5
75	595.5
76	297.0
77	265.5
78	234.0
79	152.5
80	71.0
81	81.5
82	92.0
83	58.0
84	24.0
85	13.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	596810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.72032581184061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.59886431447946	20.387805977485755
2	13.114119426510623	7.79511804182568
3	5.513268231869714	4.915683844177148
4	2.8290828311677974	3.3632505396396564
5	1.6369047163634818	2.4324670746630606
6	1.0615211174864068	1.8929252080707084
7	0.7666079259720514	1.594868613088013
8	0.5695494539337059	1.3541756269492513
9	0.4340184469766688	1.1609252687246097
>10	4.93760048197121	34.48547910606026
>50	0.4483413422481301	8.325420664773214
>100	0.07651843388558388	4.300338689861368
>500	0.0068016385676074554	1.487801289127844
>1k	0.004534425711738304	2.518750551974584
>5k	0.002267212855869152	3.984989503578836
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	6628	1.1105712035656239	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	6140	1.0288031366766643	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	5562	0.9319548935171997	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	5326	0.8924113201856537	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	4507	0.7551817161240596	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2702	0.45274040314337893	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1827	0.3061275782912485	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1289	0.2159816356964528	No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1222	0.20475528225063255	TruSeq Adapter, Index 21 (95% over 21bp)
TTGTGCGGGATATTGTGTGTAGGAGGAAACTTGAGTTTGGGGAATTATGTT	1169	0.19587473400244632	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.19336137129069553	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT	1082	0.18129723027429165	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	932	0.15616360315678357	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA	926	0.15515825807208325	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	909	0.152309780332099	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	894	0.14979641762034818	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	816	0.13672693151924398	No Hit
GCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	745	0.12483034801695683	TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	688	0.11527956971230376	No Hit
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	652	0.1092474992041018	No Hit
GGTTCGGGATGTGTGCTGTACTAGGCTTCATGATGCATTTCTAAATTTGTG	644	0.10790703909116806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.026725423501616E-4	0.0	0.0	0.4800522779444044	0.0
2	5.026725423501616E-4	0.0	0.0	2.824181900437325	0.0
3	5.026725423501616E-4	0.0	0.0	3.753623431242774	0.0
4	5.026725423501616E-4	0.0	0.0	4.878604581022436	0.0
5	5.026725423501616E-4	0.0	0.0	8.194232670364103	0.0
6	5.026725423501616E-4	0.0	0.0	9.503191970643924	0.0
7	5.026725423501616E-4	0.0	0.0	10.983897722893383	0.0
8	5.026725423501616E-4	0.0	0.0	12.999111945175182	0.0
9	5.026725423501616E-4	0.0	0.0	13.63633317136107	0.0
10	5.026725423501616E-4	0.0	0.0	16.251570851694844	0.0
11	5.026725423501616E-4	0.0	0.0	18.589500846165446	0.0
12	5.026725423501616E-4	0.0	0.0	21.515557715185736	0.0
13	5.026725423501616E-4	0.0	0.0	22.2858196075803	0.0
14	5.026725423501616E-4	0.0	0.0	22.580385717397498	0.0
15	5.026725423501616E-4	0.0	0.0	23.233524907424474	0.0
16	5.026725423501616E-4	0.0	0.0	24.38280189675106	0.0
17	5.026725423501616E-4	0.0	0.0	25.791122802902095	0.0
18	5.026725423501616E-4	0.0	0.0	27.50456594225968	0.0
19	5.026725423501616E-4	0.0	0.0	28.68081969135906	0.0
20	5.026725423501616E-4	0.0	0.0	29.604396709170423	0.0
21	5.026725423501616E-4	0.0	0.0	30.622141049915385	0.0
22	5.026725423501616E-4	0.0	0.0	31.748630217322095	0.0
23	5.026725423501616E-4	0.0	0.0	32.82300899783851	0.0
24	5.026725423501616E-4	0.0	0.0	33.67101757678323	0.0
25	5.026725423501616E-4	0.0	0.0	34.41111911663678	0.0
26	5.026725423501616E-4	0.0	0.0	35.09425110169065	0.0
27	5.026725423501616E-4	0.0	0.0	35.86903704696637	0.0
28	5.026725423501616E-4	0.0	0.0	36.50575560060991	0.0
29	5.026725423501616E-4	0.0	0.0	37.205978452103686	0.0
30	5.026725423501616E-4	0.0	0.0	38.009081617265124	0.0
31	5.026725423501616E-4	0.0	0.0	38.70008880548248	0.0
32	5.026725423501616E-4	0.0	0.0	39.35758449087649	0.0
33	5.026725423501616E-4	0.0	0.0	39.98006065582011	0.0
34	5.026725423501616E-4	0.0	0.0	40.61661165194953	0.0
35	5.026725423501616E-4	0.0	0.0	41.3428059181314	0.0
36	5.026725423501616E-4	0.0	0.0	42.011192841943	0.0
37	6.702300564668822E-4	0.0	0.0	42.65595415626414	0.0
38	8.377875705836028E-4	0.0	0.0	43.26536083510665	0.0
39	8.377875705836028E-4	0.0	0.0	43.99071731371794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCT	30	2.1637406E-6	45.000004	15
GTAAGCG	30	2.1637406E-6	45.000004	1
ATCTCGA	25	3.888543E-5	45.0	12
TTAGACG	20	7.0305576E-4	45.0	1
CGCATCG	25	3.888543E-5	45.0	21
GACGGTC	20	7.0305576E-4	45.0	29
GAACGTA	20	7.0305576E-4	45.0	1
ACGCTAG	20	7.0305576E-4	45.0	1
CGGTCTA	20	7.0305576E-4	45.0	31
CGCACCA	20	7.0305576E-4	45.0	39
AACTACG	20	7.0305576E-4	45.0	13
ACCCCTA	25	3.888543E-5	45.0	28
GTAACGT	25	3.888543E-5	45.0	13
GCGTAAG	45	3.8380676E-10	45.0	1
GTTATCG	20	7.0305576E-4	45.0	18
TGATTAG	75	0.0	42.0	1
CTATAGG	135	0.0	41.666668	2
TAGGGCA	180	0.0	41.25	5
AGGGCTC	165	0.0	40.909092	6
CTCCCAT	50	1.0804797E-9	40.5	38
>>END_MODULE