##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1007140
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12967015509264	33.0	31.0	34.0	30.0	34.0
2	32.16912941596998	33.0	31.0	34.0	30.0	34.0
3	32.18535357547113	34.0	31.0	34.0	30.0	34.0
4	35.84685644498282	37.0	35.0	37.0	35.0	37.0
5	34.88952578588876	37.0	35.0	37.0	33.0	37.0
6	35.378674265742596	37.0	35.0	37.0	33.0	37.0
7	35.929235260241875	37.0	35.0	37.0	35.0	37.0
8	35.91054868240761	37.0	35.0	37.0	35.0	37.0
9	37.802163552237026	39.0	38.0	39.0	35.0	39.0
10	37.46823976805608	39.0	37.0	39.0	35.0	39.0
11	37.39368806720019	39.0	37.0	39.0	35.0	39.0
12	37.36613281172429	39.0	37.0	39.0	35.0	39.0
13	37.32752745397859	39.0	37.0	39.0	34.0	39.0
14	38.53105625831563	40.0	38.0	41.0	34.0	41.0
15	38.57227594971901	40.0	38.0	41.0	34.0	41.0
16	38.600244256012076	40.0	38.0	41.0	34.0	41.0
17	38.52961058045555	40.0	38.0	41.0	34.0	41.0
18	38.4759278749727	40.0	38.0	41.0	34.0	41.0
19	38.35691264372381	40.0	37.0	41.0	34.0	41.0
20	38.26159421728856	40.0	37.0	41.0	34.0	41.0
21	38.239598268363885	40.0	37.0	41.0	34.0	41.0
22	38.27312885994003	40.0	37.0	41.0	34.0	41.0
23	38.246499990070895	40.0	37.0	41.0	34.0	41.0
24	38.235113291101534	40.0	37.0	41.0	34.0	41.0
25	38.11938260817761	40.0	37.0	41.0	34.0	41.0
26	38.08788053299442	40.0	37.0	41.0	34.0	41.0
27	38.094852751355326	40.0	37.0	41.0	34.0	41.0
28	37.985441944516154	40.0	36.0	41.0	34.0	41.0
29	37.92641241535437	40.0	36.0	41.0	34.0	41.0
30	37.80604980439661	40.0	36.0	41.0	33.0	41.0
31	37.739131600373334	40.0	36.0	41.0	33.0	41.0
32	37.56849395317433	40.0	36.0	41.0	33.0	41.0
33	37.354906964275074	40.0	36.0	41.0	33.0	41.0
34	37.18649244395019	40.0	36.0	41.0	32.0	41.0
35	37.10561391663522	40.0	36.0	41.0	32.0	41.0
36	37.034614849971206	40.0	36.0	41.0	31.0	41.0
37	36.97743412038049	40.0	36.0	41.0	31.0	41.0
38	36.91768274519928	40.0	35.0	41.0	31.0	41.0
39	36.85963520463888	40.0	35.0	41.0	31.0	41.0
40	36.728009015628416	40.0	35.0	41.0	31.0	41.0
41	36.651591635720955	40.0	35.0	41.0	30.0	41.0
42	36.66981055265405	40.0	35.0	41.0	31.0	41.0
43	36.57669837361241	40.0	35.0	41.0	30.0	41.0
44	36.44322040629903	39.0	35.0	41.0	30.0	41.0
45	36.42170899775602	39.0	35.0	41.0	30.0	41.0
46	36.31814643445797	39.0	35.0	41.0	30.0	41.0
47	36.20166610401731	39.0	35.0	41.0	29.0	41.0
48	36.1719204877177	39.0	35.0	41.0	29.0	41.0
49	36.153873344321546	39.0	35.0	41.0	29.0	41.0
50	36.05240085787477	39.0	35.0	41.0	29.0	41.0
51	34.98367059197331	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	11.0
9	14.0
10	23.0
11	16.0
12	18.0
13	20.0
14	22.0
15	32.0
16	66.0
17	127.0
18	284.0
19	574.0
20	1059.0
21	1781.0
22	2696.0
23	3828.0
24	5130.0
25	7652.0
26	10211.0
27	11674.0
28	11939.0
29	12096.0
30	13964.0
31	17105.0
32	21904.0
33	29999.0
34	51526.0
35	70209.0
36	66485.0
37	100266.0
38	189081.0
39	377010.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88053299441984	20.391703238874438	23.145441547351908	13.582322219353815
2	33.261810671803325	26.624004607105267	23.868280477391426	16.245904243699982
3	30.13404293345513	26.37955001290784	27.925710427547312	15.56069662608972
4	26.37518120618782	24.288480251007805	31.29703914053657	18.039299402267808
5	26.431379947177152	29.40137418829557	26.360386837976847	17.80685902655043
6	24.677006175904044	35.467263736918405	26.370613817344164	13.485116269833389
7	77.7955398455031	5.381972714816212	12.964036777409296	3.858450662271382
8	79.24886311734217	7.091764799332764	10.026014258196478	3.6333578251285816
9	73.23609428679231	7.019480906328813	11.357705979307744	8.386718827571142
10	35.7643425938797	32.941001251067384	18.542307921440912	12.75234823361201
11	26.51756458883571	24.973290704370793	29.980936116130824	18.52820859066267
12	23.930039517842605	23.040888059256908	31.85157972079353	21.177492702106957
13	23.37619397501837	24.4767361042159	33.59542863951387	18.55164128125186
14	20.005461008400026	28.684095557717892	30.486426911849396	20.824016522032686
15	18.984649601842843	26.91919693389201	34.648410350100285	19.44774311416486
16	22.43551045534881	27.359155628810296	30.455745973747444	19.749587942093452
17	22.23335385348611	26.573763329825052	30.472426872132967	20.720455944555873
18	23.09559743431896	25.615207419028142	30.972456659451513	20.316738487201384
19	21.753281569593106	27.46281549734893	29.79704906964275	20.986853863415213
20	24.002323410846554	26.815636356415197	31.48350775463193	17.698532478106323
21	23.250491490756005	28.321683182079948	30.721448855174057	17.70637647198999
22	22.496475167305437	23.872351410925987	31.702543837003795	21.92862958476478
23	20.854697460134638	27.004090791746926	32.18589272593681	19.955319022181623
24	21.565621462755924	24.984609885418116	31.647735170879916	21.802033480946044
25	20.29886609607403	30.08261016343309	29.237742518418493	20.38078122207439
26	20.3472208431797	27.310999463828267	30.104553488095004	22.237226204897034
27	21.2588120817364	27.343467641042952	29.859304565403022	21.53841571181762
28	18.354945687789183	27.838930039517845	32.210119745020556	21.59600452767242
29	20.60210099886808	27.492304942709055	30.30601505252497	21.59957900589789
30	21.833310165418908	27.37951029648311	30.751831920090556	20.035347618007428
31	22.606092499553192	26.744543956153066	27.9395118851401	22.709851659153642
32	23.03721428996962	28.87274857517326	27.863752804972496	20.226284329884624
33	22.238516988700678	27.738348193895586	27.550390213872948	22.47274460353079
34	22.00111205989237	27.47046090910896	29.567388843656296	20.961038187342375
35	21.901125960641025	27.21031832714419	28.580038524931982	22.3085171872828
36	22.87348332903072	29.399686240244655	26.880870584030024	20.8459598466946
37	22.099608793216436	27.97247651766388	28.68776932700518	21.2401453621145
38	20.801378159938043	28.943344520126296	26.86994856722998	23.385328752705682
39	22.71352542844093	27.98667513950394	27.732490021248285	21.56730941080684
40	22.631312429255118	26.680799094465517	28.53515896498997	22.152729511289394
41	22.111722302758306	26.36296840558413	28.144945092042818	23.38036419961475
42	21.49979148877018	26.612288261810672	28.342335722938223	23.545584526480926
43	22.780944059415773	26.503961713366564	27.550588795996582	23.16450543122108
44	22.16891395436583	26.384315983875133	28.171257223424746	23.275512838334294
45	21.068073951982843	26.149194749488654	28.031058244136865	24.75167305439164
46	22.860277617808844	27.706872927299088	27.299580991719125	22.133268463172946
47	20.70049844113033	26.782671723891415	30.69106579025756	21.825764044720692
48	21.559068252675896	26.693111186131024	28.729868737216275	23.017951823976805
49	21.190599122267013	25.613718053100857	30.64191671465735	22.55376610997478
50	20.977520503604268	25.824115813094505	30.217447425382765	22.980916257918462
51	20.3456321861906	24.8709216196358	28.61687550886669	26.16657068530691
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	473.0
1	882.0
2	1291.0
3	7441.0
4	13591.0
5	8883.0
6	4175.0
7	3991.0
8	3807.0
9	3867.0
10	3927.0
11	3978.0
12	4029.0
13	3967.5
14	3906.0
15	3823.0
16	3740.0
17	3727.0
18	3714.0
19	3707.0
20	3700.0
21	3851.5
22	4003.0
23	4358.5
24	4714.0
25	5597.5
26	7892.5
27	9304.0
28	10837.0
29	12370.0
30	15403.5
31	18437.0
32	21990.0
33	25543.0
34	28875.5
35	32208.0
36	35202.0
37	38196.0
38	41529.5
39	44863.0
40	47649.5
41	50436.0
42	54547.5
43	58659.0
44	62468.5
45	66278.0
46	68979.0
47	71680.0
48	90179.0
49	108678.0
50	99287.5
51	89897.0
52	86670.0
53	83443.0
54	72792.0
55	62141.0
56	55174.0
57	48207.0
58	42897.5
59	37588.0
60	33928.5
61	30269.0
62	25993.5
63	21718.0
64	18220.5
65	14723.0
66	11609.5
67	8496.0
68	6765.5
69	5035.0
70	4150.5
71	3266.0
72	2480.5
73	1695.0
74	1472.0
75	972.5
76	696.0
77	493.5
78	291.0
79	229.0
80	167.0
81	95.0
82	23.0
83	20.0
84	17.0
85	13.0
86	9.0
87	6.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1007140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.355394063103702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63238472509055	21.908630634741943
2	8.916364426922026	5.234867827250717
3	3.1312797585137586	2.7575985369897476
4	1.630643276201366	1.9147270379696615
5	1.1096871936012138	1.6287652427471642
6	0.8761712380850479	1.543221117644642
7	0.7494338463728674	1.5399948119152127
8	0.6520487614374937	1.5312918672285059
9	0.5324990408283371	1.4068547263566522
>10	7.447901758199061	48.81471509134044
>50	0.2763257538315714	4.873383861795112
>100	0.03845417265854492	1.911930870878956
>500	0.002722419303258252	0.6884909118602396
>1k	0.002722419303258252	1.200863211182812
>5k	0.001361209651629126	3.04466425009814
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8039	0.7982008459598467	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC	7815	0.7759596481124769	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC	7573	0.7519312111523722	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG	7051	0.700101276883055	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2695	0.26758941160116767	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1705	0.16929126040073872	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1693	0.16809976765891535	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1341	0.13314931389876283	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	1298	0.1288797982405624	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT	1216	0.12073793117143596	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAAGCGT	1048	0.10405703278590862	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	1025	0.10177333836408048	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10931945906229522	0.0
2	0.0	0.0	0.0	0.6187818972536092	0.0
3	0.0	0.0	0.0	0.9499175884186906	0.0
4	0.0	0.0	0.0	1.4354508807117183	0.0
5	0.0	0.0	0.0	2.9640367774092975	0.0
6	0.0	0.0	0.0	3.6972019778779512	0.0
7	0.0	0.0	0.0	4.338820819349842	0.0
8	0.0	0.0	0.0	5.076751990785789	0.0
9	0.0	0.0	0.0	5.380384057827114	0.0
10	0.0	0.0	0.0	6.674643048632762	0.0
11	0.0	0.0	0.0	7.444843815159759	0.0
12	0.0	0.0	0.0	8.85140099688226	0.0
13	0.0	0.0	0.0	9.166451536032726	0.0
14	0.0	0.0	0.0	9.309827829298806	0.0
15	0.0	0.0	0.0	9.598764819190976	0.0
16	0.0	0.0	0.0	9.9914609686836	0.0
17	0.0	0.0	0.0	10.492285084496693	0.0
18	0.0	0.0	0.0	11.030641221677225	0.0
19	0.0	0.0	0.0	11.491649621701054	0.0
20	9.929106181861508E-5	0.0	0.0	11.833508747542545	0.0
21	1.9858212363723016E-4	0.0	0.0	12.22541056854062	0.0
22	1.9858212363723016E-4	0.0	0.0	12.694560835633576	0.0
23	1.9858212363723016E-4	0.0	0.0	13.143257143991898	0.0
24	1.9858212363723016E-4	0.0	0.0	13.478066604444267	0.0
25	1.9858212363723016E-4	0.0	0.0	13.786067478205611	0.0
26	1.9858212363723016E-4	0.0	0.0	14.090493873741487	0.0
27	2.978731854558453E-4	0.0	0.0	14.41418273527017	0.0
28	2.978731854558453E-4	0.0	0.0	14.719304168238775	0.0
29	2.978731854558453E-4	0.0	0.0	15.068610123716663	0.0
30	2.978731854558453E-4	0.0	0.0	15.496256726969438	0.0
31	3.9716424727446033E-4	0.0	0.0	15.854399586949183	0.0
32	3.9716424727446033E-4	0.0	0.0	16.192187779256113	0.0
33	3.9716424727446033E-4	0.0	0.0	16.538217129693987	0.0
34	4.964553090930755E-4	0.0	0.0	16.887820958357327	0.0
35	4.964553090930755E-4	0.0	0.0	17.270786583791725	0.0
36	4.964553090930755E-4	0.0	0.0	17.619397501836886	0.0
37	4.964553090930755E-4	0.0	0.0	17.99263260321306	0.0
38	4.964553090930755E-4	0.0	0.0	18.38552733482932	0.0
39	4.964553090930755E-4	0.0	0.0	18.810095915165718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	20	7.0328685E-4	45.000004	15
CAATCGA	20	7.0328685E-4	45.000004	5
TAATGCG	20	7.0328685E-4	45.000004	1
TAGTACG	25	3.8904604E-5	45.0	1
CGTTTTT	3995	0.0	42.803505	1
TACGAAT	125	0.0	41.4	12
CGACAGG	155	0.0	40.64516	2
TACGGCT	870	0.0	40.344826	7
GTTTGCG	40	3.4576442E-7	39.375004	1
ACGATAG	40	3.4576442E-7	39.375004	1
GTATACG	40	3.4576442E-7	39.375004	1
ACGGCTG	915	0.0	38.60656	8
TCGTGCG	35	6.247521E-6	38.571426	1
CGAATAT	140	0.0	38.571426	14
AGTACCG	35	6.247521E-6	38.571426	1
CTACGAA	135	0.0	38.333332	11
TAATACG	65	9.094947E-12	38.07692	4
CGTGCGG	95	0.0	37.894737	2
TCGTCCC	95	0.0	37.894737	38
CACGAAC	30	1.1398217E-4	37.499996	42
>>END_MODULE