##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1210636
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.069218989027256	33.0	31.0	34.0	30.0	34.0
2	32.12509788243535	33.0	31.0	34.0	30.0	34.0
3	32.14963952831404	33.0	31.0	34.0	30.0	34.0
4	35.807023746196215	37.0	35.0	37.0	35.0	37.0
5	34.93315827383293	37.0	35.0	37.0	33.0	37.0
6	35.36304801773613	37.0	35.0	37.0	33.0	37.0
7	35.89918026557942	37.0	35.0	37.0	35.0	37.0
8	35.87422974370496	37.0	35.0	37.0	35.0	37.0
9	37.74897491896821	39.0	38.0	39.0	35.0	39.0
10	37.37316584010388	39.0	37.0	39.0	34.0	39.0
11	37.27398078365421	39.0	37.0	39.0	34.0	39.0
12	37.116698165261894	39.0	37.0	39.0	34.0	39.0
13	36.993092060701976	39.0	37.0	39.0	33.0	39.0
14	37.92101837381343	40.0	37.0	41.0	33.0	41.0
15	38.006797253674925	40.0	37.0	41.0	33.0	41.0
16	38.16511982131706	40.0	37.0	41.0	33.0	41.0
17	38.12126849028114	40.0	37.0	41.0	33.0	41.0
18	38.110849173492284	40.0	37.0	41.0	33.0	41.0
19	38.042312470470065	40.0	37.0	41.0	34.0	41.0
20	37.93664900102095	40.0	36.0	41.0	33.0	41.0
21	37.914344195943286	40.0	36.0	41.0	33.0	41.0
22	38.01101569753419	40.0	36.0	41.0	34.0	41.0
23	38.003203274972826	40.0	36.0	41.0	34.0	41.0
24	37.9626906848962	40.0	36.0	41.0	34.0	41.0
25	37.86195272567477	40.0	36.0	41.0	34.0	41.0
26	37.84742978071031	40.0	36.0	41.0	34.0	41.0
27	37.83973465186894	40.0	36.0	41.0	34.0	41.0
28	37.74360831827238	40.0	36.0	41.0	33.0	41.0
29	37.69058494873769	40.0	36.0	41.0	33.0	41.0
30	37.58659084976822	40.0	35.0	41.0	33.0	41.0
31	37.50706570761154	40.0	35.0	41.0	33.0	41.0
32	37.43572799751536	40.0	35.0	41.0	33.0	41.0
33	37.27116325633799	40.0	35.0	41.0	32.0	41.0
34	37.08923656656501	40.0	35.0	41.0	32.0	41.0
35	37.05059819797197	40.0	35.0	41.0	32.0	41.0
36	36.95634030377421	39.0	35.0	41.0	31.0	41.0
37	36.956750831794196	39.0	35.0	41.0	31.0	41.0
38	36.812795092827244	39.0	35.0	41.0	31.0	41.0
39	36.77675948840114	39.0	35.0	41.0	31.0	41.0
40	36.65295431492208	39.0	35.0	41.0	31.0	41.0
41	36.468040765349784	39.0	35.0	41.0	30.0	41.0
42	36.572883178758936	39.0	35.0	41.0	31.0	41.0
43	36.47405000346925	39.0	35.0	41.0	31.0	41.0
44	36.428880357101555	39.0	35.0	41.0	31.0	41.0
45	36.41207266263353	39.0	35.0	41.0	31.0	41.0
46	36.31298011954047	39.0	35.0	41.0	31.0	41.0
47	36.154100819734424	38.0	35.0	41.0	30.0	41.0
48	36.08422597708973	38.0	35.0	40.0	30.0	41.0
49	36.09431654105776	38.0	35.0	40.0	30.0	41.0
50	35.95441734757598	38.0	35.0	40.0	30.0	41.0
51	34.73404061997165	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	20.0
10	21.0
11	29.0
12	29.0
13	34.0
14	47.0
15	55.0
16	93.0
17	159.0
18	279.0
19	611.0
20	1177.0
21	2149.0
22	3216.0
23	4608.0
24	5927.0
25	7843.0
26	9578.0
27	11304.0
28	12888.0
29	14830.0
30	18142.0
31	23483.0
32	31656.0
33	45644.0
34	83535.0
35	91408.0
36	86041.0
37	129279.0
38	228049.0
39	398217.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7567823854569	20.715227368094126	24.499601862161708	12.028388384287267
2	30.86526420823435	28.603312638976536	24.266170839129188	16.26525231365993
3	30.439620166590124	27.846850746219342	26.227040993329126	15.486488093861409
4	27.629526959383334	23.566373377299204	31.063011508000756	17.74108815531671
5	27.70477666284498	28.838230483811817	24.771112043587006	18.685880809756195
6	24.85288724273853	35.61722929105032	25.05955547332146	14.470327992889686
7	77.57269732603359	5.239642634119587	12.746605916229155	4.441054123617668
8	79.12716952081385	7.526374566756647	8.82660023326582	4.519855679163679
9	73.89520879934184	5.507848767094321	10.37719017111667	10.219752262447177
10	43.421143927654555	20.748267852599792	19.351646572545338	16.478941647200315
11	32.12584129333673	23.496327550147196	24.38949444754658	19.988336708969502
12	27.667110510508525	20.31180305227996	29.191020257121053	22.830066180090462
13	27.54048285364056	20.440247935795732	32.75402350500068	19.26524570556303
14	22.647434901985402	25.787272144558727	30.413022576563066	21.152270376892808
15	20.06556884150149	24.03026177975874	35.504478637674744	20.399690741065026
16	25.109777009770074	24.11005454983992	28.037659544239556	22.742508896150454
17	24.997108957605754	24.154659203922566	28.694504376212173	22.153727462259507
18	25.090448326334258	24.081639733165048	29.40297496522489	21.424936975275806
19	24.992896295831283	25.669152412450973	27.192566551795917	22.145384739921823
20	24.326552324563288	27.703289841042228	28.69268714956436	19.27747068483012
21	23.794187517965764	27.17125543928976	30.72674197694435	18.307815065800128
22	23.039625453067643	21.763106334191285	31.318166649595753	23.879101563145323
23	22.178507825638754	25.357085036294976	30.699979184494765	21.76442795357151
24	23.39208482153182	24.91549896087676	28.206000812795917	23.486415404795498
25	23.516564846906913	28.252587895948906	26.173350206007424	22.057497051136757
26	20.806501706541024	24.40601468979941	28.606864491060897	26.18061911259867
27	22.328181220449416	25.69963225940745	28.670219620100507	23.301966900042622
28	19.832716026947818	25.39417298015258	29.750973868280806	25.022137124618794
29	22.134398778823694	24.493984979795744	29.3455671233963	24.026049117984265
30	23.28346422871945	24.392220287518295	29.471946976630463	22.852368507131786
31	25.068806808983048	24.18034818062572	27.026868522000008	23.723976488391227
32	26.474679424699083	24.571630118384054	26.329549096507954	22.62414136040891
33	25.047826101321952	22.976352925239297	27.230397906554906	24.745423066883852
34	22.778357821839098	24.248163775073596	30.521395365741643	22.45208303734566
35	22.798429916176293	24.67612065063322	28.0011498088608	24.52429962432969
36	24.9098820785108	24.901208951328062	29.15384971205218	21.03505925810896
37	22.24153254983331	25.62182191839661	27.970091753425475	24.166553778344603
38	24.01638477626636	25.33726074559157	27.008861457944423	23.63749302019765
39	22.08236001572727	24.623173274212892	28.17205171496635	25.12241499509349
40	24.056776768574533	22.465877439626773	30.793070749589475	22.68427504220922
41	21.524636637271648	24.61218731311476	28.619832881229367	25.243343168384218
42	22.529562973511442	22.94017359470559	30.871128894234108	23.65913453754886
43	22.034038307137735	22.238806709861596	30.545019312163195	25.182135670837475
44	22.994359989294882	22.314221615745776	29.23091664216164	25.460501752797704
45	21.72056671039024	21.950445881338403	28.145206321305494	28.18378108696586
46	24.11443241403692	24.057602780687176	27.965796490439736	23.86216831483617
47	19.913417410352903	23.82367615038707	32.67299171675053	23.58991472250949
48	21.983238562210275	25.441007866939362	28.428858880786628	24.146894690063736
49	22.807516049415348	22.139437452710805	31.430669499337537	23.622376998536307
50	21.918892218635495	22.436884414473056	29.74040091323899	25.90382245365246
51	21.362407858348835	22.722932409080844	28.02758219646533	27.887077536104986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	1020.5
2	1509.0
3	4752.5
4	7996.0
5	5219.0
6	2442.0
7	2349.0
8	2256.0
9	2287.5
10	2319.0
11	2345.5
12	2372.0
13	2323.5
14	2275.0
15	2249.0
16	2223.0
17	2178.0
18	2133.0
19	2187.5
20	2242.0
21	2598.0
22	2954.0
23	3241.5
24	3529.0
25	3988.0
26	6121.5
27	7796.0
28	9451.0
29	11106.0
30	13064.0
31	15022.0
32	16842.5
33	18663.0
34	23411.5
35	28160.0
36	29689.0
37	31218.0
38	34717.5
39	38217.0
40	42716.0
41	47215.0
42	51621.0
43	56027.0
44	64363.5
45	72700.0
46	106068.0
47	139436.0
48	128745.0
49	118054.0
50	118986.0
51	119918.0
52	106166.0
53	92414.0
54	84628.5
55	76843.0
56	73362.0
57	69881.0
58	65551.5
59	61222.0
60	54782.0
61	48342.0
62	43529.0
63	38716.0
64	33483.0
65	28250.0
66	23905.5
67	19561.0
68	16193.0
69	12825.0
70	10215.0
71	7605.0
72	6785.5
73	5966.0
74	4548.5
75	2378.5
76	1626.0
77	1163.0
78	700.0
79	578.5
80	457.0
81	313.0
82	169.0
83	138.0
84	107.0
85	64.0
86	21.0
87	19.0
88	17.0
89	12.0
90	7.0
91	4.5
92	2.0
93	5.5
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1210636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.34776405260724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04726390889464	20.707266019778775
2	10.663871845565884	6.04593845930686
3	3.843986160664577	3.26905238112021
4	1.9020291706769548	2.1567309660610614
5	1.1775653894390816	1.6690672908167818
6	0.8262573098620705	1.4053528360027177
7	0.6395387121878312	1.269064476092625
8	0.5149286628811944	1.1677660991424508
9	0.41178298864897867	1.0505814302788823
>10	6.51497932591866	45.721079926598534
>50	0.3959406495745153	6.725064730356724
>100	0.05426977772465903	2.7585813138288744
>500	0.002917729984974427	0.5477130444756293
>1k	0.0037930489804667545	2.4561067435372714
>5k	0.0	0.0
>10k+	8.75318995492328E-4	3.050634282602633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	12795	1.0568824981249525	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	12329	1.018390333675853	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	11759	0.9713076432552807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4890	0.40391992308175206	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	4366	0.3606368883793312	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2817	0.2326876121311443	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	2407	0.19882111551283785	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	2311	0.19089139923147833	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2100	0.17346254365474015	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT	1852	0.15297744326122797	No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	1795	0.14826917421917077	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	1792	0.1480213705853783	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	1746	0.1442217148672268	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1565	0.1292708956284135	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4780363379248594E-4	0.0	0.0	0.15471206869777537	0.0
2	2.4780363379248594E-4	0.0	0.0	0.9005184052018939	0.0
3	2.4780363379248594E-4	0.0	0.0	1.2619813056938667	0.0
4	2.4780363379248594E-4	0.0	0.0	1.8155746235862802	0.0
5	2.4780363379248594E-4	0.0	0.0	3.77760119474392	0.0
6	2.4780363379248594E-4	0.0	0.0	4.5844498263722535	0.0
7	2.4780363379248594E-4	0.0	0.0	5.443750227153331	0.0
8	2.4780363379248594E-4	0.0	0.0	6.5022021482923025	0.0
9	2.4780363379248594E-4	0.0	0.0	6.891006049712713	0.0
10	2.4780363379248594E-4	0.0	0.0	8.732434852424676	0.0
11	2.4780363379248594E-4	0.0	0.0	9.862336821307148	0.0
12	2.4780363379248594E-4	0.0	0.0	11.867316022322152	0.0
13	2.4780363379248594E-4	0.0	0.0	12.288747402191905	0.0
14	2.4780363379248594E-4	0.0	0.0	12.502601938154822	0.0
15	2.4780363379248594E-4	0.0	0.0	12.91321255934897	0.0
16	2.4780363379248594E-4	0.0	0.0	13.482665309804103	0.0
17	3.304048450566479E-4	0.0	0.0	14.14925708470589	0.0
18	3.304048450566479E-4	0.0	0.0	14.869209242084326	0.0
19	3.304048450566479E-4	0.0	0.0	15.494913417410354	0.0
20	3.304048450566479E-4	0.0	0.0	15.989281666826363	0.0
21	3.304048450566479E-4	0.0	0.0	16.53321064300087	0.0
22	3.304048450566479E-4	0.0	0.0	17.194185535536693	0.0
23	4.130060563208099E-4	0.0	0.0	17.754882557597824	0.0
24	4.130060563208099E-4	0.0	0.0	18.218523156423565	0.0
25	4.130060563208099E-4	0.0	0.0	18.635328868462526	0.0
26	4.130060563208099E-4	0.0	0.0	19.05337359867045	0.0
27	4.130060563208099E-4	0.0	0.0	19.566740126677217	0.0
28	4.956072675849719E-4	0.0	0.0	19.98495005930767	0.0
29	4.956072675849719E-4	0.0	0.0	20.47717067723081	0.0
30	4.956072675849719E-4	0.0	0.0	21.06206985419234	0.0
31	5.782084788491338E-4	0.0	0.0	21.511668247103177	0.0
32	5.782084788491338E-4	0.0	0.0	21.945655011085083	0.0
33	5.782084788491338E-4	0.0	0.0	22.37922876901067	0.0
34	5.782084788491338E-4	0.0	0.0	22.858233193131543	0.0
35	5.782084788491338E-4	0.0	0.0	23.34979300136457	0.0
36	5.782084788491338E-4	0.0	0.0	23.788818439233594	0.0
37	5.782084788491338E-4	0.0	0.0	24.266914250030563	0.0
38	5.782084788491338E-4	0.0	0.0	24.803243914768768	0.0
39	6.608096901132958E-4	0.0	0.0	25.697732431548378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTC	40	6.8157533E-9	45.000004	30
ATATACG	25	3.8909282E-5	45.000004	1
CCCTTCG	25	3.8909282E-5	45.000004	33
GATTACG	20	7.0334354E-4	45.000004	1
AATCGGC	20	7.0334354E-4	45.000004	27
CGTTTTT	2210	0.0	41.538464	1
TGTTACG	45	1.9284926E-8	40.000004	1
GCGATTA	45	1.9284926E-8	40.000004	9
CTATGCG	40	3.4584264E-7	39.375004	1
CCGATCG	40	3.4584264E-7	39.375004	40
TACGGCT	1470	0.0	39.336735	7
CGTAAGG	195	0.0	39.23077	2
CGATCGA	35	6.248565E-6	38.571426	41
TAATGCG	65	9.094947E-12	38.07692	1
TTACGGG	395	0.0	37.594936	3
AGGGCGA	790	0.0	37.594936	6
TTGTCGT	30	1.139958E-4	37.499996	27
TCGACGG	30	1.139958E-4	37.499996	2
GTTTGCG	85	0.0	37.058826	1
ACGGCTG	1575	0.0	36.714287	8
>>END_MODULE