FastQCFastQC Report
Sat 14 Jan 2017
SRR2935767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165489
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222901.9125019626954867No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC62930.5399450359462852No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT45830.39322550448781585No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC36730.31514668950114505No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG32000.27456286588719414No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG25890.222138518681858No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT25840.22170951420390927No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC24670.21167080941990873No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT20610.17683564581047098No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT17740.15221078877621325No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC14810.12707112636841703No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC14730.12638471920369904No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC14150.12140826725949366No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT13920.11943484666092945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA207.0333283E-445.024
GTTCACG253.8908394E-545.01
TATAGCG406.8157533E-945.01
CGTTTTT108300.044.0858731
CGAATAT1250.041.39999814
ACTACGG603.6379788E-1241.2500042
CGTTATT2850.041.052631
CTACGAA1300.039.80769311
CGTTCTG4350.039.3103451
GTTTTTT124050.039.0689242
TCGTTGA700.038.5714324
TAATCGT700.038.5714321
CTCGATC1700.038.38235516
TCGATCA1650.038.18181617
ACGATAG301.13993156E-437.5000041
TATTACG301.13993156E-437.5000041
ACGTATG301.13993156E-437.5000041
ATACGAG301.13993156E-437.5000041
CGTTTCT6150.037.3170741
TCCACCG1100.036.8181838
Produced by FastQC (version 0.11.5)