##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1165489
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08754265377022	33.0	31.0	34.0	30.0	34.0
2	32.1990992621981	34.0	31.0	34.0	30.0	34.0
3	32.10802933361018	33.0	31.0	34.0	30.0	34.0
4	35.81654910513956	37.0	35.0	37.0	35.0	37.0
5	34.99988073675513	37.0	35.0	37.0	33.0	37.0
6	35.41356374877841	37.0	35.0	37.0	33.0	37.0
7	35.915833611471236	37.0	35.0	37.0	35.0	37.0
8	35.894223797908005	37.0	35.0	37.0	35.0	37.0
9	37.760510824211984	39.0	38.0	39.0	35.0	39.0
10	37.513727714289885	39.0	37.0	39.0	35.0	39.0
11	37.38576425860733	39.0	37.0	39.0	35.0	39.0
12	37.193883425755196	39.0	37.0	39.0	34.0	39.0
13	37.0815666213924	39.0	37.0	39.0	33.0	39.0
14	38.00910090099521	40.0	37.0	41.0	33.0	41.0
15	38.00217333668529	40.0	37.0	41.0	33.0	41.0
16	38.06427087685941	40.0	37.0	41.0	33.0	41.0
17	38.0401839914405	40.0	37.0	41.0	33.0	41.0
18	37.925500798377335	39.0	37.0	41.0	33.0	41.0
19	37.8129317393815	39.0	37.0	41.0	34.0	41.0
20	37.668050921115515	39.0	35.0	41.0	33.0	41.0
21	37.61228205500009	39.0	35.0	41.0	33.0	41.0
22	37.59040111060679	39.0	35.0	41.0	33.0	41.0
23	37.557487887058564	39.0	35.0	41.0	33.0	41.0
24	37.55712237524335	39.0	35.0	41.0	33.0	41.0
25	37.449901286069625	39.0	35.0	41.0	33.0	41.0
26	37.45320118851401	39.0	35.0	41.0	33.0	41.0
27	37.47066510280234	39.0	35.0	41.0	33.0	41.0
28	37.338004048086255	39.0	35.0	41.0	33.0	41.0
29	37.24691610131026	39.0	35.0	41.0	33.0	41.0
30	37.056107779653004	39.0	35.0	41.0	33.0	41.0
31	36.86936813646461	39.0	35.0	41.0	32.0	41.0
32	36.59524971921657	39.0	35.0	41.0	31.0	41.0
33	36.23379628636564	39.0	35.0	41.0	30.0	41.0
34	35.92005930557903	39.0	35.0	41.0	29.0	41.0
35	35.71076518096696	39.0	35.0	41.0	27.0	41.0
36	35.57041121795229	39.0	35.0	41.0	26.0	41.0
37	35.50622785800638	39.0	35.0	41.0	26.0	41.0
38	35.40442252136228	39.0	35.0	41.0	25.0	41.0
39	35.33292206104047	39.0	35.0	41.0	24.0	41.0
40	35.186743933233174	38.0	35.0	41.0	23.0	41.0
41	35.040637878178174	38.0	35.0	41.0	23.0	41.0
42	35.03598060556556	38.0	35.0	41.0	23.0	41.0
43	34.920786897173635	38.0	35.0	40.0	23.0	41.0
44	34.785597290064516	38.0	34.0	40.0	23.0	41.0
45	34.771422124104134	38.0	34.0	40.0	23.0	41.0
46	34.625898657130186	38.0	34.0	40.0	23.0	41.0
47	34.478946605244666	37.0	34.0	40.0	23.0	41.0
48	34.39624741203048	37.0	34.0	40.0	22.0	41.0
49	34.375106929366126	37.0	34.0	40.0	22.0	41.0
50	34.27933425369094	37.0	34.0	40.0	22.0	41.0
51	33.25844259362379	35.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	22.0
10	32.0
11	33.0
12	27.0
13	30.0
14	56.0
15	81.0
16	120.0
17	219.0
18	477.0
19	862.0
20	1545.0
21	2481.0
22	3691.0
23	5439.0
24	8474.0
25	13647.0
26	19758.0
27	23415.0
28	23637.0
29	21910.0
30	22577.0
31	26127.0
32	31521.0
33	43742.0
34	80203.0
35	97266.0
36	86291.0
37	120551.0
38	204383.0
39	326664.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.0689616118213	20.51250590953668	23.16529799938052	22.253234479261494
2	39.098266907709984	23.639605350200647	21.38184058365201	15.880287158437358
3	28.546472768082754	22.87915201258871	32.556720827052	16.017654392276548
4	25.944732211114818	22.49725222631874	33.738370761114005	17.81964480145244
5	24.21798918737114	27.265122193345455	30.78673415193108	17.730154467352328
6	24.86767356877671	30.06772264688899	30.638127000769632	14.426476783564668
7	72.6247952576129	5.2210702975317655	17.364728453035593	4.789405991819742
8	73.69241580143614	5.5595548306333225	16.40847747168785	4.339551896242693
9	68.20991017504241	5.776373693788616	18.09480827360876	7.918907857560217
10	43.72928444627105	19.725711696978692	23.302751034115293	13.242252822634962
11	32.971911360810786	22.728485639933112	27.44393125975449	16.855671739501616
12	29.629280070425374	20.178654624796973	31.5406666214782	18.651398683299455
13	27.144829337728627	20.700667273565003	34.34987374398214	17.804629644724233
14	23.165898605649645	21.85957997029573	37.191685206810185	17.782836217244437
15	22.34924568142642	19.759688851632234	40.0388163251648	17.85224914177654
16	26.20719715072386	20.729067370005207	36.14319826270347	16.920537216567467
17	26.22392832536386	20.746913956287877	34.53305865606625	18.496099062282013
18	25.827356585948042	24.035404881556154	32.94574208765591	17.191496444839892
19	22.788632067741524	29.571621868589066	29.950690225304573	17.689055838364844
20	22.281720376597292	31.085149666792223	30.24472989449064	16.38840006211985
21	25.07822896655395	27.976583219575645	31.37095245000167	15.574235363868727
22	23.727551268180136	22.464476284203457	34.78565649268247	19.022315954933937
23	20.10134801787061	22.496051013780484	38.41220294657436	18.990398021774553
24	21.110623952692816	21.354041093480934	38.39941861313149	19.135916340694763
25	21.986565295768557	21.137651234803588	36.948525468708844	19.92725800071901
26	18.67070388480715	21.100499447013227	35.775627225996985	24.453169442182638
27	19.45415186243714	22.550706184271153	33.18958823292198	24.805553720369733
28	18.431490987902933	23.890401368009478	34.861075479905864	22.81703216418173
29	19.816489044512647	21.01075171022635	33.79971840146068	25.373040843800325
30	21.942206232748656	20.49002607489217	29.548541427675424	28.01922626468375
31	21.779956739188442	23.90370050682589	29.271833539398486	25.04450921458718
32	22.716730917237314	26.52397405724121	28.489929977889112	22.269365047632366
33	23.73595975594793	23.892031585025684	29.68616606420138	22.685842594825004
34	23.892031585025684	21.589307149188024	31.694507627270614	22.82415363851568
35	24.286715704738526	23.985211357636153	27.285113802017865	24.44295913560746
36	22.176700080395438	26.788240815657634	27.443845458858902	23.591213645088025
37	20.996079757080505	26.07326195270826	28.696538534469223	24.234119755742007
38	21.769403229030903	22.570955195630333	29.79358878547974	25.866052789859022
39	20.80053951603147	21.34134256093365	30.6158187679163	27.242299155118584
40	21.268154396995595	21.22654096263457	31.97104391375637	25.534260726613468
41	19.123732613521017	21.84679563685286	35.75246098418775	23.27701076543837
42	22.089183166893896	21.2806813277517	35.13117669922239	21.498958806132016
43	23.080440913642256	20.00130417361296	34.30997632753291	22.60827858521187
44	21.31800471733324	19.367836161473853	37.024802464888126	22.28935665630478
45	21.39350950545222	19.11489512127528	36.45783014683107	23.03376522644143
46	25.224347891743292	20.386807597497704	32.90841869807437	21.480425812684633
47	26.34670940695279	20.460081562331347	32.013172153490935	21.180036877224925
48	24.006575780638	20.134381362672663	31.34169434460557	24.51734851208377
49	22.12255971527831	18.717465372903565	33.864412276735344	25.295562635082785
50	20.428592633649913	19.071994673480404	36.98481924754331	23.514593445326383
51	20.0236124064663	19.70572008830628	36.184296891690956	24.086370613536463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	788.0
1	1192.0
2	1596.0
3	19042.5
4	36489.0
5	23431.0
6	10373.0
7	10007.0
8	9641.0
9	9512.5
10	9384.0
11	9344.5
12	9305.0
13	9042.0
14	8779.0
15	8356.5
16	7934.0
17	7294.5
18	6655.0
19	6108.0
20	5561.0
21	5258.0
22	4955.0
23	4981.5
24	5008.0
25	5293.0
26	5883.5
27	6189.0
28	7009.0
29	7829.0
30	9566.0
31	11303.0
32	12638.0
33	13973.0
34	15830.5
35	17688.0
36	19957.0
37	22226.0
38	26936.0
39	31646.0
40	40633.0
41	49620.0
42	58272.0
43	66924.0
44	71006.0
45	75088.0
46	75402.0
47	75716.0
48	89130.0
49	102544.0
50	98586.5
51	94629.0
52	92000.5
53	89372.0
54	80472.5
55	71573.0
56	66750.5
57	61928.0
58	58761.0
59	55594.0
60	51306.5
61	47019.0
62	43278.5
63	39538.0
64	35679.5
65	31821.0
66	27596.5
67	23372.0
68	21286.5
69	19201.0
70	15620.5
71	12040.0
72	9866.5
73	7693.0
74	6030.5
75	3437.5
76	2507.0
77	1775.5
78	1044.0
79	828.0
80	612.0
81	413.5
82	215.0
83	150.0
84	85.0
85	54.5
86	24.0
87	32.0
88	40.0
89	23.0
90	6.0
91	8.5
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1165489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.073830754327663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11671073001406	22.409424637832632
2	7.727903391405169	4.80271124140638
3	3.3483457820686056	3.121377904169003
4	2.1752626179232197	2.7037496974224737
5	1.6092173744687472	2.500227417058269
6	1.322638640994999	2.4659669567647526
7	1.1312984426806778	2.460764343834569
8	1.020528568001573	2.53693856016298
9	0.9545681340290533	2.6695879776263847
>10	8.456187758220262	44.608598432368424
>50	0.09886242894731993	2.0317179836819035
>100	0.03117409175859578	1.8589410522451573
>500	0.0028084767260469785	0.63410476794163
>1k	0.003931867416465769	2.7014468884713945
>5k	2.808476726046978E-4	0.5491909310014694
>10k+	2.808476726046978E-4	1.9452512080125144
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22290	1.9125019626954867	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	6293	0.5399450359462852	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	4583	0.39322550448781585	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	3673	0.31514668950114505	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	3200	0.27456286588719414	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	2589	0.222138518681858	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	2584	0.22170951420390927	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	2467	0.21167080941990873	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	2061	0.17683564581047098	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	1774	0.15221078877621325	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1481	0.12707112636841703	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1473	0.12638471920369904	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1415	0.12140826725949366	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	1392	0.11943484666092945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.14805373538489E-4	0.0	0.0	0.06580928691733684	0.0
2	5.14805373538489E-4	0.0	0.0	0.3691154528270966	0.0
3	6.006062691282371E-4	0.0	0.0	0.8063568167524533	0.0
4	6.006062691282371E-4	0.0	0.0	1.345100640160482	0.0
5	6.006062691282371E-4	0.0	0.0	2.8371782144662028	0.0
6	6.006062691282371E-4	0.0	0.0	4.311666605176025	0.0
7	6.006062691282371E-4	0.0	0.0	5.362212770776901	0.0
8	6.006062691282371E-4	0.0	0.0	6.4870625119584995	0.0
9	6.006062691282371E-4	0.0	0.0	7.333574147846956	0.0
10	6.006062691282371E-4	0.0	0.0	8.835347223354317	0.0
11	6.006062691282371E-4	0.0	0.0	10.240251087740853	0.0
12	6.006062691282371E-4	0.0	0.0	11.208085189993213	0.0
13	6.006062691282371E-4	0.0	0.0	11.663087339305648	0.0
14	6.006062691282371E-4	0.0	0.0	11.916714786668944	0.0
15	6.864071647179853E-4	0.0	0.0	12.15275305043634	0.0
16	6.864071647179853E-4	0.0	0.0	12.396942399284764	0.0
17	6.864071647179853E-4	0.0	0.0	12.637356508727239	0.0
18	6.864071647179853E-4	0.0	0.0	12.941349081801716	0.0
19	6.864071647179853E-4	0.0	0.0	13.170694875713112	0.0
20	6.864071647179853E-4	0.0	0.0	13.381421875281534	0.0
21	6.864071647179853E-4	0.0	0.0	13.623466201740214	0.0
22	6.864071647179853E-4	0.0	0.0	13.912529418982075	0.0
23	6.864071647179853E-4	0.0	0.0	14.233682171174502	0.0
24	6.864071647179853E-4	0.0	0.0	14.489797844509901	0.0
25	6.864071647179853E-4	0.0	0.0	14.690657741085502	0.0
26	6.864071647179853E-4	0.0	0.0	14.900784134384795	0.0
27	6.864071647179853E-4	0.0	0.0	15.13373356591096	0.0
28	7.722080603077334E-4	0.0	0.0	15.349265415632408	0.0
29	7.722080603077334E-4	0.0	0.0	15.585475281190986	0.0
30	7.722080603077334E-4	0.0	0.0	15.89718993486854	0.0
31	7.722080603077334E-4	0.0	0.0	16.14601253207881	0.0
32	7.722080603077334E-4	0.0	0.0	16.39080248719636	0.0
33	7.722080603077334E-4	0.0	0.0	16.65386803307453	0.0
34	7.722080603077334E-4	0.0	0.0	16.924827261346955	0.0
35	7.722080603077334E-4	0.0	0.0	17.193727268125226	0.0
36	8.580089558974817E-4	0.0	0.0	17.48742373372893	0.0
37	8.580089558974817E-4	0.0	0.0	17.77803136709141	0.0
38	8.580089558974817E-4	0.0	0.0	18.12492438796076	0.0
39	8.580089558974817E-4	0.0	0.0	18.61287408117966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTA	20	7.0333283E-4	45.0	24
GTTCACG	25	3.8908394E-5	45.0	1
TATAGCG	40	6.8157533E-9	45.0	1
CGTTTTT	10830	0.0	44.085873	1
CGAATAT	125	0.0	41.399998	14
ACTACGG	60	3.6379788E-12	41.250004	2
CGTTATT	285	0.0	41.05263	1
CTACGAA	130	0.0	39.807693	11
CGTTCTG	435	0.0	39.310345	1
GTTTTTT	12405	0.0	39.068924	2
TCGTTGA	70	0.0	38.57143	24
TAATCGT	70	0.0	38.57143	21
CTCGATC	170	0.0	38.382355	16
TCGATCA	165	0.0	38.181816	17
ACGATAG	30	1.13993156E-4	37.500004	1
TATTACG	30	1.13993156E-4	37.500004	1
ACGTATG	30	1.13993156E-4	37.500004	1
ATACGAG	30	1.13993156E-4	37.500004	1
CGTTTCT	615	0.0	37.317074	1
TCCACCG	110	0.0	36.81818	38
>>END_MODULE