##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990669
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06285954239004	33.0	31.0	34.0	30.0	34.0
2	32.08692812634694	33.0	31.0	34.0	30.0	34.0
3	32.12251922690626	33.0	31.0	34.0	30.0	34.0
4	35.781102467120704	37.0	35.0	37.0	35.0	37.0
5	34.854153102600364	37.0	35.0	37.0	33.0	37.0
6	35.34314084724565	37.0	35.0	37.0	33.0	37.0
7	35.91994702569678	37.0	35.0	37.0	35.0	37.0
8	35.90858298785972	37.0	35.0	37.0	35.0	37.0
9	37.75459916480681	39.0	38.0	39.0	35.0	39.0
10	37.36597491190297	39.0	37.0	39.0	34.0	39.0
11	37.29083982642033	39.0	37.0	39.0	34.0	39.0
12	37.224930829570724	39.0	37.0	39.0	34.0	39.0
13	37.19103555274264	39.0	37.0	39.0	34.0	39.0
14	38.31152887594141	40.0	38.0	41.0	34.0	41.0
15	38.39100345322201	40.0	38.0	41.0	34.0	41.0
16	38.398684121538075	40.0	38.0	41.0	34.0	41.0
17	38.369453369389774	40.0	37.0	41.0	34.0	41.0
18	38.35764922491771	40.0	37.0	41.0	34.0	41.0
19	38.30263084844686	40.0	37.0	41.0	34.0	41.0
20	38.316210560742284	40.0	37.0	41.0	34.0	41.0
21	38.22892005301468	40.0	37.0	41.0	34.0	41.0
22	38.27181328980719	40.0	37.0	41.0	34.0	41.0
23	38.26222381037461	40.0	37.0	41.0	34.0	41.0
24	38.228166017105615	40.0	37.0	41.0	34.0	41.0
25	38.08849575387945	40.0	37.0	41.0	34.0	41.0
26	38.118739962590936	40.0	37.0	41.0	34.0	41.0
27	38.13192701093907	40.0	37.0	41.0	34.0	41.0
28	38.01367661650864	40.0	37.0	41.0	34.0	41.0
29	37.98152157784285	40.0	37.0	41.0	34.0	41.0
30	37.901726005355975	40.0	36.0	41.0	34.0	41.0
31	37.865435377507524	40.0	36.0	41.0	33.0	41.0
32	37.838601995217374	40.0	37.0	41.0	33.0	41.0
33	37.73282801823818	40.0	36.0	41.0	33.0	41.0
34	37.61468058453429	40.0	36.0	41.0	33.0	41.0
35	37.57214670086578	40.0	36.0	41.0	33.0	41.0
36	37.51414347274417	40.0	36.0	41.0	33.0	41.0
37	37.483917433572664	40.0	36.0	41.0	33.0	41.0
38	37.39453944758542	40.0	36.0	41.0	33.0	41.0
39	37.3660455712251	40.0	36.0	41.0	33.0	41.0
40	37.24792842008784	40.0	35.0	41.0	32.0	41.0
41	37.174728390612806	40.0	35.0	41.0	32.0	41.0
42	37.18713616757969	40.0	35.0	41.0	32.0	41.0
43	37.093871918875024	39.0	35.0	41.0	32.0	41.0
44	36.95006808530397	39.0	35.0	41.0	32.0	41.0
45	36.94813908580969	39.0	35.0	41.0	32.0	41.0
46	36.843521902875736	39.0	35.0	41.0	31.0	41.0
47	36.72973717760423	39.0	35.0	41.0	31.0	41.0
48	36.70021874107295	39.0	35.0	41.0	31.0	41.0
49	36.690150797087625	39.0	35.0	41.0	31.0	41.0
50	36.55807540157207	39.0	35.0	41.0	31.0	41.0
51	35.38956402188824	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	22.0
10	37.0
11	26.0
12	14.0
13	17.0
14	23.0
15	35.0
16	52.0
17	123.0
18	200.0
19	378.0
20	663.0
21	1136.0
22	1702.0
23	2568.0
24	3471.0
25	4606.0
26	6133.0
27	7251.0
28	8541.0
29	10481.0
30	13059.0
31	16818.0
32	22436.0
33	32003.0
34	57433.0
35	75813.0
36	69861.0
37	106028.0
38	192626.0
39	356852.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75915467224674	21.737129152118417	25.2106404863784	11.293075689256451
2	31.46388955342299	26.074602112309964	24.9654526385705	17.496055695696544
3	31.434212638126358	25.48934104125596	26.496539207343723	16.579907113273958
4	27.986037717946154	25.600175235118893	27.314875099553937	19.098911947381012
5	25.460168835403145	30.923850448535283	24.502230311032243	19.11375040502933
6	25.46773947706045	35.13554981532682	25.043884486140172	14.352826221472561
7	80.23749607588407	5.582288332429903	9.499742093474207	4.680473498211814
8	80.80963470139875	6.144332769068176	7.825519926433551	5.220512603099522
9	74.03320382489005	7.649881039984091	10.261348644199021	8.055566490926838
10	38.931772367965486	28.781560743295692	17.892858260428053	14.393808628310767
11	27.829375906584335	24.897619689321054	27.2015173584719	20.071487045622703
12	24.977767548999715	21.700386304608298	31.786903597467976	21.53494254892401
13	24.197587690742317	23.52945332901302	32.89181351187935	19.381145468365315
14	20.497865583762085	27.21564922289887	30.668871237517276	21.617613955821774
15	18.821119869502326	25.1331171158076	35.823468787253866	20.22229422743621
16	22.11858854975779	25.9481219256886	29.936235008867744	21.997054515685864
17	22.303816915639835	25.746540973826775	28.302288655443945	23.64735345508944
18	23.004454565551157	24.820399144416548	30.379470842430724	21.79567544760157
19	22.832146761430913	26.876282592874105	28.271905146925967	22.019665498769015
20	24.098967465419832	26.546101674726874	29.335428886944076	20.019501972909215
21	23.495940621943355	26.74748074281117	30.376644469545326	19.379934165700146
22	22.553345264664586	23.96259497369959	29.062280135948537	24.421779625687286
23	21.129559923647555	26.005658802284117	29.439802799926113	23.424978474142222
24	22.594933322835377	24.379686858072677	29.95077064084977	23.074609178242177
25	20.959674724857646	28.12776012977089	27.164269801517964	23.7482953438535
26	19.966204655641796	27.356059390169673	28.3956599025507	24.282076051637834
27	20.75698341221942	27.43105921352137	28.112618846456282	23.69933852780293
28	18.410387324121377	25.943781424471744	31.8233436193118	23.82248763209508
29	20.468592436020508	25.493479658695286	29.937143485866624	24.100784419417586
30	21.121787398212724	25.440687050871684	29.98519182491831	23.45233372599728
31	22.31905914084321	26.64108799205385	27.912955790480975	23.12689707662196
32	22.411521910951084	26.918274418599957	27.947074148883228	22.72312952156573
33	22.937025383856767	24.513031093129996	28.468338062460823	24.081605460552414
34	22.09173800734655	25.08799609153007	30.000333108232923	22.81993279289046
35	20.967750075958772	25.29210059061099	28.267362761931587	25.472786571498652
36	22.955295865723063	26.3674345316145	28.767933588312545	21.909336014349897
37	22.100015242225204	25.295633556717732	30.13216321495878	22.472187986098284
38	20.76818796187223	25.48853350614585	29.63290463313175	24.11037389885017
39	22.873532935824176	24.859362713479477	30.46698745998916	21.800116890707187
40	22.50600351883424	24.12258786739062	30.218064762296994	23.15334385147814
41	22.09920770711509	24.624672822103044	27.330521092312367	25.945598378469498
42	22.07255904848138	24.21929019682659	28.93791972899122	24.770231025700813
43	22.75744976374551	23.758490474618668	29.018067588669876	24.465992172965944
44	20.996821339922818	24.267136652100753	29.684586880178948	25.051455127797478
45	21.349209473598144	23.63534137032652	29.26113565681373	25.75431349926161
46	23.363101096329856	24.338906335012	28.577254360437237	23.720738208220908
47	19.963983934088983	23.991767179552404	31.611971304239862	24.43227758211875
48	20.820273976474482	23.450012062555707	30.048886156728432	25.680827804241375
49	21.695238268281333	21.985143372811706	32.515804976233234	23.803813382673727
50	21.028113325439676	22.279187094781406	31.31681722149376	25.375882358285157
51	20.898302056489097	22.033999246973508	28.9590165837429	28.108682112794487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	847.0
2	1290.0
3	2360.0
4	3430.0
5	2378.5
6	1327.0
7	1314.5
8	1302.0
9	1327.0
10	1352.0
11	1394.5
12	1437.0
13	1441.5
14	1446.0
15	1417.5
16	1389.0
17	1647.0
18	1905.0
19	1969.0
20	2033.0
21	2350.0
22	2667.0
23	3188.5
24	3710.0
25	4496.5
26	6280.5
27	7278.0
28	9315.0
29	11352.0
30	12890.5
31	14429.0
32	17771.0
33	21113.0
34	23562.5
35	26012.0
36	28641.0
37	31270.0
38	34614.5
39	37959.0
40	43575.5
41	49192.0
42	54514.0
43	59836.0
44	67284.5
45	74733.0
46	90182.0
47	105631.0
48	106745.0
49	107859.0
50	103889.5
51	99920.0
52	85078.0
53	70236.0
54	63157.0
55	56078.0
56	51357.0
57	46636.0
58	41465.0
59	36294.0
60	34205.5
61	32117.0
62	28590.5
63	25064.0
64	21099.0
65	17134.0
66	14315.5
67	11497.0
68	9560.0
69	7623.0
70	6394.0
71	5165.0
72	4232.5
73	3300.0
74	2567.0
75	1465.5
76	1097.0
77	828.5
78	560.0
79	384.5
80	209.0
81	186.5
82	164.0
83	107.5
84	51.0
85	38.0
86	25.0
87	18.5
88	12.0
89	10.5
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	990669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.49976723326294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3381467957157	20.331205785302398
2	11.905251093817796	6.785937700547123
3	4.375420827885767	3.74095425426945
4	2.204522345142387	2.513134947883399
5	1.2343992679517515	1.7590045904767544
6	0.8527540691904985	1.4581975487488206
7	0.6087905023122291	1.2145271326803826
8	0.4931622058247325	1.1244006459397908
9	0.3726978960502813	0.955962295718386
>10	5.86632192073464	42.17183731949269
>50	0.6914748248922399	12.031761268441906
>100	0.04956935510630806	2.513209968229943
>500	0.004635982851668963	0.9178582668668805
>1k	0.002852912524103977	2.4820082754020696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	4638	0.4681684800876983	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	4282	0.4322331676876939	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	4143	0.41820224514948995	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3383	0.3414864096888062	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3174	0.3203895549371183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1872	0.1889632157663155	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1775	0.1791718525562019	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1154	0.11648693963372227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.037675550562297E-4	0.0	0.0	0.18724720365732653	0.0
2	4.037675550562297E-4	0.0	0.0	1.0966326795327197	0.0
3	4.037675550562297E-4	0.0	0.0	1.5324997552159199	0.0
4	4.037675550562297E-4	0.0	0.0	2.1333058771395894	0.0
5	4.037675550562297E-4	0.0	0.0	3.846087845688116	0.0
6	4.037675550562297E-4	0.0	0.0	4.681180091433163	0.0
7	4.037675550562297E-4	0.0	0.0	5.462167484800675	0.0
8	4.037675550562297E-4	0.0	0.0	6.70445930981993	0.0
9	4.037675550562297E-4	0.0	0.0	7.125286044077285	0.0
10	5.04709443820287E-4	0.0	0.0	8.495067474605545	0.0
11	5.04709443820287E-4	0.0	0.0	9.981335844767527	0.0
12	5.04709443820287E-4	0.0	0.0	11.634461157056494	0.0
13	5.04709443820287E-4	0.0	0.0	12.133719738883523	0.0
14	5.04709443820287E-4	0.0	0.0	12.337016702854333	0.0
15	5.04709443820287E-4	0.0	0.0	12.736645640471236	0.0
16	6.056513325843445E-4	0.0	0.0	13.476549685111777	0.0
17	7.065932213484019E-4	0.0	0.0	14.42570626516021	0.0
18	7.065932213484019E-4	0.0	0.0	15.454606937332247	0.0
19	7.065932213484019E-4	0.0	0.0	16.147371119919974	0.0
20	8.075351101124594E-4	0.0	0.0	16.776642854475107	0.0
21	8.075351101124594E-4	0.0	0.0	17.51775820178082	0.0
22	8.075351101124594E-4	0.0	0.0	18.301370084256195	0.0
23	8.075351101124594E-4	0.0	0.0	19.056920121655164	0.0
24	9.084769988765167E-4	0.0	0.0	19.65964413946535	0.0
25	9.084769988765167E-4	0.0	0.0	20.18484478670474	0.0
26	9.084769988765167E-4	0.0	0.0	20.690159881857614	0.0
27	9.084769988765167E-4	0.0	0.0	21.21949914653633	0.0
28	9.084769988765167E-4	0.0	0.0	21.734908430565607	0.0
29	9.084769988765167E-4	0.0	0.0	22.29644815776006	0.0
30	9.084769988765167E-4	0.0	0.0	22.926224601759014	0.0
31	0.001009418887640574	0.0	0.0	23.492205772059084	0.0
32	0.0011103607764046317	0.0	0.0	24.01407533696926	0.0
33	0.001211302665168689	0.0	0.0	24.57258680750079	0.0
34	0.001211302665168689	0.0	0.0	25.118783367603104	0.0
35	0.001211302665168689	0.0	0.0	25.687187143233512	0.0
36	0.0013122445539327464	0.0	0.0	26.210469894586385	0.0
37	0.0013122445539327464	0.0	0.0	26.74818733603252	0.0
38	0.0013122445539327464	0.0	0.0	27.292365058359554	0.0
39	0.0014131864426968038	0.0	0.0	27.876919536192208	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTG	20	7.032815E-4	45.0	34
CGACACG	25	3.8904145E-5	45.0	37
CGTTCGG	40	6.8139343E-9	45.0	2
ACTAGCG	20	7.032815E-4	45.0	1
TATAGCG	20	7.032815E-4	45.0	1
TAGTACG	25	3.8904145E-5	45.0	1
AGTCGAC	20	7.032815E-4	45.0	31
TCGATTG	60	3.6379788E-12	41.250004	1
CGTTTTT	1125	0.0	39.999996	1
ATGGGCG	260	0.0	39.807693	5
ACGGGTA	80	0.0	39.375	5
CGTGATA	35	6.2474173E-6	38.571426	19
GCTACGA	125	0.0	37.800003	10
CGGATTC	30	1.139808E-4	37.500004	14
TATGTCG	30	1.139808E-4	37.500004	1
ACGGTTT	30	1.139808E-4	37.500004	11
CGCGACC	145	0.0	37.241383	10
ACGCGAG	55	2.748493E-9	36.81818	1
CGACGGT	55	2.748493E-9	36.81818	28
TATTGCG	55	2.748493E-9	36.81818	1
>>END_MODULE