FastQCFastQC Report
Sat 14 Jan 2017
SRR2935765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730234
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153230.8856027566213587No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC53190.30741506640142313No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC44390.25655489373113693No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT41020.2370777594244478No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG40010.2312403986975172No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC36050.20835332099588844No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC33780.19523370827298506TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG31810.18384796507293233TruSeq Adapter, Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC26050.15055767023419953TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT25030.14466251385650725No Hit
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT24680.14263966607984815TruSeq Adapter, Index 8 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT20430.11807651450613038TruSeq Adapter, Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG207.034277E-445.01
CGTTTTT78050.042.3190271
CGTTGAT750.039.025
ACGTTAG700.038.5714261
ATAACGC1000.038.2511
TACGGGT1300.038.076924
GCGATAT659.094947E-1238.076929
CACGACC3850.037.98701527
CGTATGG1750.037.2857132
TACGGGA5550.036.891894
CGGTCTA1650.036.81818431
AGCGACT10450.036.38755819
GACACGA4050.036.1111125
CGCGTAT250.002107402336.024
GCCGTAT250.002107402336.09
CGTAAGG2200.035.7954562
TATGGGA9750.035.769234
TTACACG17800.035.64606534
TCACGAC1900.035.52631425
TGTTACG950.035.5263141
Produced by FastQC (version 0.11.5)