FastQCFastQC Report
Sat 14 Jan 2017
SRR2935764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754967
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC222392.945691665993348No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG188612.4982548906111126No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC176682.3402347387369247No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC71190.94295512254178No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT39960.5292946579122001No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT36520.483729752426265No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA31930.4229323930714852No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT31740.42041572678011097No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC31230.41366046462957984No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC30520.40425608006707575No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT22360.29617188565857844No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT16140.213784178646219No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.17947804341116896No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC12710.1683517292808825No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG10470.13868155826678516No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC9740.1290122614630838No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT8410.11139559742346355No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTAT8300.10993858009687842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA900.045.00000414
TGATCCG207.031748E-445.04
GTTTGCG207.031748E-445.01
TCCGCAT207.031748E-445.039
CTCGTCG207.031748E-445.030
ATTAGCG207.031748E-445.01
TTGCTCG351.2111923E-745.01
CGCATCG207.031748E-445.021
CGCGCCA207.031748E-445.020
GTTAGTC207.031748E-445.020
TTATCCG253.889529E-545.01
TACGCGG253.889529E-545.02
CGCTCGT207.031748E-445.024
CTACGAA406.8102963E-945.011
CGAATAT406.8102963E-945.014
GTTCACG207.031748E-445.01
CCGAACG207.031748E-445.01
GACGCGA207.031748E-445.01
ATACGTT207.031748E-445.037
TCGAATA406.8102963E-945.029