##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754967
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.886829490560515	33.0	31.0	34.0	30.0	34.0
2	31.910931206264646	33.0	31.0	34.0	30.0	34.0
3	31.980420336253108	33.0	31.0	34.0	30.0	34.0
4	35.67928399519449	37.0	35.0	37.0	33.0	37.0
5	34.697029141671095	37.0	35.0	37.0	33.0	37.0
6	35.174591737122284	37.0	35.0	37.0	33.0	37.0
7	35.73692889887902	37.0	35.0	37.0	33.0	37.0
8	35.6464507720205	37.0	35.0	37.0	33.0	37.0
9	37.56532802095985	39.0	37.0	39.0	35.0	39.0
10	37.190803041722354	39.0	37.0	39.0	34.0	39.0
11	37.13218458555142	39.0	37.0	39.0	34.0	39.0
12	37.02739589942342	39.0	37.0	39.0	33.0	39.0
13	36.84846887347394	39.0	37.0	39.0	33.0	39.0
14	37.92771207218329	40.0	37.0	41.0	33.0	41.0
15	37.891159481142886	40.0	37.0	41.0	33.0	41.0
16	38.11541961436725	40.0	37.0	41.0	34.0	41.0
17	37.97190340769861	40.0	36.0	41.0	33.0	41.0
18	37.989777036612196	40.0	37.0	41.0	33.0	41.0
19	37.912212056950835	40.0	37.0	41.0	33.0	41.0
20	37.79480957445822	40.0	36.0	41.0	33.0	41.0
21	37.891571419677945	40.0	36.0	41.0	33.0	41.0
22	37.95395957704112	40.0	36.0	41.0	33.0	41.0
23	37.899725418462	40.0	36.0	41.0	33.0	41.0
24	37.84282359361403	40.0	36.0	41.0	33.0	41.0
25	37.78928482966805	40.0	36.0	41.0	33.0	41.0
26	37.759499421829034	40.0	36.0	41.0	33.0	41.0
27	37.77421662138875	40.0	36.0	41.0	33.0	41.0
28	37.65431999014527	40.0	36.0	41.0	33.0	41.0
29	37.56865266958688	40.0	36.0	41.0	33.0	41.0
30	37.54008320893496	40.0	36.0	41.0	33.0	41.0
31	37.48443441898785	40.0	36.0	41.0	33.0	41.0
32	37.32490426733884	40.0	35.0	41.0	32.0	41.0
33	37.24679754214423	40.0	35.0	41.0	32.0	41.0
34	37.0631246133937	39.0	35.0	41.0	31.0	41.0
35	37.09326367907472	39.0	35.0	41.0	32.0	41.0
36	37.080614119557545	39.0	35.0	41.0	32.0	41.0
37	37.05353743938477	39.0	35.0	41.0	32.0	41.0
38	36.95110647220342	39.0	35.0	41.0	31.0	41.0
39	36.85153788178821	39.0	35.0	41.0	31.0	41.0
40	36.76791965741549	39.0	35.0	41.0	31.0	41.0
41	36.544739041574005	39.0	35.0	41.0	30.0	41.0
42	36.78660656690955	39.0	35.0	41.0	31.0	41.0
43	36.734275802783436	39.0	35.0	41.0	31.0	41.0
44	36.66172826097035	39.0	35.0	41.0	31.0	41.0
45	36.647793877083366	39.0	35.0	41.0	31.0	41.0
46	36.52877013167463	39.0	35.0	41.0	31.0	41.0
47	36.30257878821193	39.0	35.0	40.0	30.0	41.0
48	36.29663415751947	39.0	35.0	40.0	30.0	41.0
49	36.360610463768616	39.0	35.0	40.0	30.0	41.0
50	36.2196864233801	39.0	35.0	40.0	30.0	41.0
51	35.04883657166472	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	19.0
11	11.0
12	12.0
13	9.0
14	14.0
15	24.0
16	37.0
17	77.0
18	214.0
19	400.0
20	755.0
21	1419.0
22	2294.0
23	3045.0
24	3682.0
25	4490.0
26	5347.0
27	6118.0
28	7143.0
29	8838.0
30	11467.0
31	15283.0
32	20955.0
33	29519.0
34	48241.0
35	63191.0
36	55123.0
37	82799.0
38	146263.0
39	238011.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.38529366184217	19.368131322296207	21.984139704119517	10.262435311742104
2	27.449941520622755	34.1693080624716	22.731192224295896	15.649558192609744
3	26.98859685257766	33.47907921803205	24.738697188089017	14.793626741301274
4	25.663373365988186	22.581251895778227	35.477179797262664	16.27819494097093
5	30.460536685709442	27.251124883604184	23.307508805020618	18.980829625665756
6	22.140437926425925	39.319864311950056	24.32662619690662	14.213071564717398
7	71.6433963338795	5.012934340176458	18.57008319568935	4.7735861302547
8	70.15631146791847	13.20614013592647	10.377142312180531	6.260406083974532
9	65.82102264072469	5.873899124067675	11.064722034208117	17.240356200999514
10	41.15040789862338	21.332985415256562	20.790445145284494	16.72616154083556
11	31.08996817079422	24.00224115756053	24.80108402089098	20.106706650754273
12	25.010232235316245	19.7804672257198	30.274965660750734	24.93433487821322
13	23.615204373171277	23.351616693179967	35.88858850784206	17.14459042580669
14	20.284595220718256	29.942633254168726	27.897510752125587	21.875260772987428
15	15.976459898247208	24.18238148157469	39.85882826666596	19.98233035351214
16	18.146223609773674	25.59701284956826	28.21129930182379	28.045464238834278
17	18.20145781206331	25.947359288551684	31.39660408998009	24.454578809404914
18	18.897647181929806	25.405878667544407	31.595420726998665	24.101053423527123
19	19.16467872105668	27.411661701769745	27.844263391644937	25.57939618552864
20	21.00966002487526	27.19999682105311	33.586103763475755	18.20423939059588
21	20.209095231977024	31.72098912932618	29.279028089969493	18.790887548727294
22	18.038934152088768	23.86991749308248	30.87353486973603	27.217613485092723
23	18.33457621326495	29.24737107714642	30.06396306063709	22.354089648951543
24	22.445087003802815	24.090059565517432	27.382918723599843	26.081934707079913
25	16.89067204261908	34.1106300010464	26.17902504347872	22.819672912855793
26	16.663112427430605	27.177346824430735	29.259292128000297	26.900248620138363
27	21.126883691605062	28.442700144509626	25.71834265603662	24.712073507848686
28	16.841265909635787	26.97230474974403	32.22313028251566	23.963299058104525
29	20.30764258570242	27.451663450190537	27.11986086809092	25.120833096016113
30	20.768987253747515	28.263354557219056	27.907444961170487	23.06021322786294
31	25.37938744342468	26.328435547513994	24.03151396021283	24.260663048848492
32	23.5150675459987	32.28882851833259	22.851992206281864	21.344111729386846
33	23.7108376922435	23.621827179201212	25.093017310690403	27.574317817864884
34	22.41647648175351	26.20379433803067	29.164983370134056	22.214745810081766
35	18.64068230796843	24.626241941700762	25.422833050981037	31.31024269934977
36	23.534803507967897	26.510165344975345	28.951464103729034	21.003567043327724
37	18.675253355444674	25.676883890289243	31.133546234471176	24.514316519794903
38	24.96201820741834	25.42442252442822	24.318678829670702	25.294880438482743
39	22.459524720948068	26.589374105093334	31.108114659316232	19.842986514642362
40	23.24247284980668	22.59582206904408	28.887222885238696	25.274482195910547
41	18.796583161913038	24.127279735405654	31.449586538219553	25.62655056446176
42	18.926125247858515	22.51608348444369	31.157918160661325	27.399873107036466
43	22.631320309364515	22.842322909478163	29.443141223391223	25.083215557766103
44	21.679755538983823	22.70840977155293	29.474268411731902	26.137566277731345
45	20.256117154789546	22.006524788500688	27.54080641935343	30.196551637356333
46	26.874552132742224	24.685317371487763	26.73070478577209	21.70942570999792
47	18.095757827825587	22.212626512152188	36.540405077308016	23.15121058271421
48	20.861441625925373	23.863029774811352	28.444157161836216	26.831371437427066
49	19.97014439041706	19.82629704344693	35.42379998066141	24.779758585474596
50	22.20732826732824	19.586683921283978	31.794767188499627	26.411220622888155
51	20.153993485807987	20.427647830964794	27.116417008955356	32.30194167427186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	419.0
1	632.0
2	845.0
3	1744.5
4	2644.0
5	1835.5
6	1027.0
7	1034.5
8	1042.0
9	1071.0
10	1100.0
11	1117.0
12	1134.0
13	1141.0
14	1148.0
15	1131.5
16	1115.0
17	1279.5
18	1444.0
19	1645.0
20	1846.0
21	2065.5
22	2285.0
23	2452.0
24	2619.0
25	3092.5
26	3973.0
27	4380.0
28	5735.0
29	7090.0
30	8365.0
31	9640.0
32	11175.0
33	12710.0
34	15464.0
35	18218.0
36	20152.0
37	22086.0
38	23172.0
39	24258.0
40	27560.0
41	30862.0
42	35439.0
43	40016.0
44	46990.5
45	53965.0
46	98026.0
47	142087.0
48	115334.0
49	88581.0
50	84371.5
51	80162.0
52	65825.0
53	51488.0
54	44467.0
55	37446.0
56	33243.5
57	29041.0
58	25854.0
59	22667.0
60	20424.0
61	18181.0
62	16216.0
63	14251.0
64	12219.0
65	10187.0
66	7912.5
67	5638.0
68	4624.0
69	3610.0
70	2859.5
71	2109.0
72	2041.5
73	1974.0
74	1439.5
75	809.5
76	714.0
77	534.0
78	354.0
79	199.0
80	44.0
81	33.5
82	23.0
83	27.0
84	31.0
85	20.0
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	754967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.54871663789809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.60831057107752	18.745600296322415
2	12.426703618908977	6.5982606624311435
3	4.933128069916943	3.929046578000581
4	2.426058736572022	2.5763498377659064
5	1.413381062605621	1.8761726666243965
6	0.9296136728610589	1.4808029990102367
7	0.6502422591360868	1.2084168238653468
8	0.4938128573391672	1.0488078097318674
9	0.36954677015801113	0.8829893236837943
>10	4.926756930834733	32.67025630453414
>50	0.7377450964464262	12.154478861276745
>100	0.07317308701145675	3.597231830424731
>500	0.0040094842198058495	0.77732673768601
>1k	0.005513040802233043	3.687445375127079
>5k	5.011855274757312E-4	0.9472422193746499
>10k+	0.0015035565824271936	7.81957167414095
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	22239	2.945691665993348	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	18861	2.4982548906111126	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	17668	2.3402347387369247	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	7119	0.94295512254178	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	3996	0.5292946579122001	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	3652	0.483729752426265	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	3193	0.4229323930714852	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	3174	0.42041572678011097	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	3123	0.41366046462957984	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	3052	0.40425608006707575	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2236	0.29617188565857844	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	1614	0.213784178646219	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1355	0.17947804341116896	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1271	0.1683517292808825	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG	1047	0.13868155826678516	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	974	0.1290122614630838	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT	841	0.11139559742346355	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTAT	830	0.10993858009687842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9736836179594606E-4	0.0	0.0	0.3110069711656271	0.0
2	3.9736836179594606E-4	0.0	0.0	2.0557189916910277	0.0
3	3.9736836179594606E-4	0.0	0.0	2.6333601336217343	0.0
4	3.9736836179594606E-4	0.0	0.0	3.7044003247824078	0.0
5	3.9736836179594606E-4	0.0	0.0	7.733053232790307	0.0
6	3.9736836179594606E-4	0.0	0.0	8.844889909095365	0.0
7	3.9736836179594606E-4	0.0	0.0	10.286939694052853	0.0
8	3.9736836179594606E-4	0.0	0.0	12.047149080688294	0.0
9	3.9736836179594606E-4	0.0	0.0	12.498294627447292	0.0
10	3.9736836179594606E-4	0.0	0.0	16.237398455826547	0.0
11	3.9736836179594606E-4	0.0	0.0	17.923432414926744	0.0
12	3.9736836179594606E-4	0.0	0.0	22.4072045533116	0.0
13	3.9736836179594606E-4	0.0	0.0	23.149621109267027	0.0
14	3.9736836179594606E-4	0.0	0.0	23.477582463869282	0.0
15	3.9736836179594606E-4	0.0	0.0	24.305830585972632	0.0
16	3.9736836179594606E-4	0.0	0.0	25.270905880654386	0.0
17	3.9736836179594606E-4	0.0	0.0	26.357443437925102	0.0
18	3.9736836179594606E-4	0.0	0.0	27.45338537975832	0.0
19	3.9736836179594606E-4	0.0	0.0	28.68721414313473	0.0
20	3.9736836179594606E-4	0.0	0.0	29.431087716416744	0.0
21	3.9736836179594606E-4	0.0	0.0	30.202247250542076	0.0
22	3.9736836179594606E-4	0.0	0.0	31.100167292080314	0.0
23	3.9736836179594606E-4	0.0	0.0	31.947356639429273	0.0
24	3.9736836179594606E-4	0.0	0.0	32.581689000976205	0.0
25	5.298244823945948E-4	0.0	0.0	33.16091961635409	0.0
26	6.622806029932435E-4	0.0	0.0	33.70783093830591	0.0
27	6.622806029932435E-4	0.0	0.0	34.295406289281516	0.0
28	6.622806029932435E-4	0.0	0.0	34.85013252234866	0.0
29	9.271928441905408E-4	0.0	0.0	35.42817103264116	0.0
30	0.0010596489647891896	0.0	0.0	36.039456029203926	0.0
31	0.0010596489647891896	0.0	0.0	36.599877875456805	0.0
32	0.0010596489647891896	0.0	0.0	37.1299672700926	0.0
33	0.0010596489647891896	0.0	0.0	37.65078473628649	0.0
34	0.0010596489647891896	0.0	0.0	38.163257466882655	0.0
35	0.0010596489647891896	0.0	0.0	38.71122843779927	0.0
36	0.0010596489647891896	0.0	0.0	39.23535730700812	0.0
37	0.0010596489647891896	0.0	0.0	39.76147301802595	0.0
38	0.0010596489647891896	0.0	0.0	40.26917732828058	0.0
39	0.0010596489647891896	0.0	0.0	40.817810579800174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGGTA	90	0.0	45.000004	14
TGATCCG	20	7.031748E-4	45.0	4
GTTTGCG	20	7.031748E-4	45.0	1
TCCGCAT	20	7.031748E-4	45.0	39
CTCGTCG	20	7.031748E-4	45.0	30
ATTAGCG	20	7.031748E-4	45.0	1
TTGCTCG	35	1.2111923E-7	45.0	1
CGCATCG	20	7.031748E-4	45.0	21
CGCGCCA	20	7.031748E-4	45.0	20
GTTAGTC	20	7.031748E-4	45.0	20
TTATCCG	25	3.889529E-5	45.0	1
TACGCGG	25	3.889529E-5	45.0	2
CGCTCGT	20	7.031748E-4	45.0	24
CTACGAA	40	6.8102963E-9	45.0	11
CGAATAT	40	6.8102963E-9	45.0	14
GTTCACG	20	7.031748E-4	45.0	1
CCGAACG	20	7.031748E-4	45.0	1
GACGCGA	20	7.031748E-4	45.0	1
ATACGTT	20	7.031748E-4	45.0	37
TCGAATA	40	6.8102963E-9	45.0	29
>>END_MODULE