##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935762.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140977
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.089398822237435	33.0	31.0	34.0	30.0	34.0
2	32.13266437447906	33.0	31.0	34.0	30.0	34.0
3	32.15983319558589	34.0	31.0	34.0	30.0	34.0
4	35.80977793592684	37.0	35.0	37.0	35.0	37.0
5	34.83727717561353	37.0	35.0	37.0	33.0	37.0
6	35.3312590876065	37.0	35.0	37.0	33.0	37.0
7	35.910666910901796	37.0	35.0	37.0	35.0	37.0
8	35.916649503013645	37.0	35.0	37.0	35.0	37.0
9	37.77341348686257	39.0	38.0	39.0	35.0	39.0
10	37.41502940024208	39.0	37.0	39.0	35.0	39.0
11	37.30673799734789	39.0	37.0	39.0	34.0	39.0
12	37.08526727532632	39.0	37.0	39.0	34.0	39.0
13	36.93138161417802	39.0	37.0	39.0	33.0	39.0
14	37.764968093134215	40.0	37.0	41.0	33.0	41.0
15	37.967745186800435	40.0	37.0	41.0	33.0	41.0
16	38.06676909350495	40.0	37.0	41.0	33.0	41.0
17	38.110827825626636	40.0	37.0	41.0	33.0	41.0
18	38.10864986761346	40.0	36.0	41.0	34.0	41.0
19	38.040940351996575	40.0	36.0	41.0	34.0	41.0
20	38.01151031090022	40.0	36.0	41.0	33.0	41.0
21	37.92454361481432	40.0	36.0	41.0	33.0	41.0
22	38.027492228151836	40.0	36.0	41.0	34.0	41.0
23	38.04272391117437	40.0	36.0	41.0	34.0	41.0
24	38.00241722663998	40.0	36.0	41.0	34.0	41.0
25	37.84148584940801	40.0	35.0	41.0	34.0	41.0
26	37.85130638040907	40.0	35.0	41.0	34.0	41.0
27	37.84922307811639	40.0	35.0	41.0	34.0	41.0
28	37.739827358483126	40.0	35.0	41.0	33.0	41.0
29	37.69615250789455	40.0	35.0	41.0	33.0	41.0
30	37.5533363073927	40.0	35.0	41.0	33.0	41.0
31	37.46367017038906	40.0	35.0	41.0	33.0	41.0
32	37.435242778776434	40.0	35.0	41.0	33.0	41.0
33	37.28189350004426	40.0	35.0	41.0	33.0	41.0
34	37.152819907850905	39.0	35.0	41.0	32.0	41.0
35	37.09124285590332	39.0	35.0	41.0	32.0	41.0
36	37.00329016272896	39.0	35.0	41.0	32.0	41.0
37	36.937140713616486	39.0	35.0	41.0	32.0	41.0
38	36.83567591634187	39.0	35.0	41.0	31.0	41.0
39	36.75344638848986	39.0	35.0	41.0	31.0	41.0
40	36.607088486446266	39.0	35.0	41.0	31.0	41.0
41	36.47029870014908	39.0	35.0	41.0	31.0	41.0
42	36.52812721027681	39.0	35.0	41.0	31.0	41.0
43	36.4285441336679	39.0	35.0	41.0	31.0	41.0
44	36.311978243207356	39.0	35.0	41.0	31.0	41.0
45	36.26409121305688	39.0	35.0	41.0	31.0	41.0
46	36.17427695737951	38.0	35.0	41.0	30.0	41.0
47	36.05764358089602	38.0	35.0	40.0	30.0	41.0
48	35.99205680745536	38.0	35.0	40.0	30.0	41.0
49	35.951787809920795	38.0	35.0	40.0	30.0	41.0
50	35.796697917661795	38.0	35.0	40.0	30.0	41.0
51	34.58154371209937	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	22.0
10	39.0
11	27.0
12	27.0
13	23.0
14	31.0
15	56.0
16	90.0
17	192.0
18	360.0
19	581.0
20	987.0
21	1582.0
22	2407.0
23	3400.0
24	4846.0
25	6867.0
26	8695.0
27	10229.0
28	12130.0
29	14249.0
30	17893.0
31	23033.0
32	31084.0
33	45655.0
34	84262.0
35	92108.0
36	81540.0
37	121334.0
38	211925.0
39	364976.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81365882046702	21.070538669929366	24.5716609537265	11.544141555877111
2	32.56332073302091	24.905410012647057	25.330133736262873	17.20113551806916
3	32.2922372668336	24.42888857531747	26.05512643988441	17.223747717964518
4	29.190684825373342	25.452835596160135	26.430331198613118	18.926148379853405
5	24.7484392761642	31.05592838418303	25.11391553028676	19.081716809366007
6	26.5957157769175	34.151608665205345	24.579198353691616	14.673477204185536
7	80.26401934482466	5.871809861197903	9.099745218352343	4.764425575625101
8	81.68464394987805	5.29949332896281	8.151960994831622	4.863901726327525
9	75.73334081230384	6.693123524838801	10.52028217922009	7.053253483637269
10	43.7127128767714	21.761963650450447	18.36496265919471	16.160360813583445
11	33.15711009073802	24.22888454368493	23.15953783468028	19.454467530896764
12	30.030841988927033	21.04932877700427	28.489969561174327	20.42985967289437
13	27.85998315478752	21.918408521819458	30.862585310659195	19.35902301273382
14	21.257746650458333	25.578342069997905	32.34701488285916	20.816896396684594
15	20.66080210205815	23.429043705526055	35.49493109852346	20.41522309389234
16	25.971864463525556	23.5499926817105	29.664839869690624	20.813302985073317
17	26.296323238768178	23.51116630747158	27.411420212677378	22.78109024108286
18	26.418937454479803	23.50170073542236	29.24572537395583	20.833636436142008
19	26.136986109273018	26.041015725996232	26.61701331402824	21.204984850702512
20	24.70777237402682	28.605309309477754	27.0769700002717	19.609948316223726
21	24.072877893244122	26.89957816853451	30.163710574358642	18.863833363862724
22	22.117273179038666	23.567959739766884	30.980379096160572	23.334387985033878
23	22.144355232401704	24.744933508738566	30.778972757557778	22.331738501301952
24	23.466204840237793	26.065030232861837	28.43992473117337	22.028840195726996
25	25.039242684120715	25.39709389409252	25.962924756590184	23.600738665196584
26	21.63514251382806	25.516815851677993	26.465038296126913	26.383003338367033
27	21.47685711456059	25.312079034020847	29.769399383160223	23.441664468258345
28	20.014601521327773	26.047939616661857	28.76035187387651	25.17710698813385
29	23.32579885484107	26.822538929356156	27.410894347563534	22.440767868239238
30	24.62144285117053	25.8746670616498	27.84359369207267	21.660296395107
31	24.35316399892373	27.00256008666257	27.1140434907978	21.5302324236159
32	26.811408117779763	25.564406644481004	25.69736287409825	21.926822363640987
33	25.70034277641004	25.26930867142808	25.584301874621485	23.446046677540387
34	21.940582500786608	26.580553332801628	29.14274345582777	22.336120710583998
35	22.420171484613626	27.174868555632585	27.0422629027579	23.36269705699589
36	25.5605503003128	28.07085506543953	24.436075398540023	21.93251923570764
37	22.229633025030303	29.870716061761104	26.51131442614531	21.38833648706328
38	22.973644516935924	27.01903719356306	26.080718542091557	23.92659974740946
39	22.505712209799146	24.603651081485427	26.715174801946052	26.175461906769375
40	24.672101190470975	23.61441115815656	27.4228139568107	24.290673694561765
41	21.733479290117156	24.967637384452097	26.5567141143073	26.74216921112345
42	22.16030647418835	24.856066336131228	28.45526246366053	24.528364726019895
43	22.541733970097557	23.882514722032084	29.287093429578338	24.288657878292025
44	22.705716241431688	23.700302460084647	27.942193400918686	25.651787897564983
45	21.273172027131135	23.921253452085363	27.440518082310163	27.365056438473346
46	22.55838636536933	24.840027450158946	27.196691957857173	25.40489422661456
47	20.19190570887932	25.80516522243656	30.008668009959887	23.994261058724234
48	20.5124204957681	26.58782780020982	28.22800109029367	24.67175061372841
49	23.247269664506824	24.177086829971156	29.057991528313014	23.517651977209006
50	21.170102464817433	24.018275565589843	28.90847054760964	25.90315142198309
51	20.54826696769523	24.95142321010853	26.97714327282671	27.52316654936953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	565.0
1	1144.5
2	1724.0
3	3802.5
4	5881.0
5	3920.0
6	1959.0
7	1996.0
8	2033.0
9	2041.5
10	2050.0
11	2042.0
12	2034.0
13	2068.5
14	2103.0
15	2153.5
16	2204.0
17	2189.0
18	2174.0
19	2513.0
20	2852.0
21	2880.0
22	2908.0
23	3335.5
24	3763.0
25	4122.5
26	6367.0
27	8252.0
28	8549.0
29	8846.0
30	11825.5
31	14805.0
32	17065.0
33	19325.0
34	22780.5
35	26236.0
36	29443.0
37	32650.0
38	35453.5
39	38257.0
40	43024.0
41	47791.0
42	51844.5
43	55898.0
44	63424.5
45	70951.0
46	85174.0
47	99397.0
48	106627.0
49	113857.0
50	113079.0
51	112301.0
52	99880.0
53	87459.0
54	79763.0
55	72067.0
56	68224.0
57	64381.0
58	62097.5
59	59814.0
60	53298.0
61	46782.0
62	43015.5
63	39249.0
64	33850.0
65	28451.0
66	24150.0
67	19849.0
68	16916.5
69	13984.0
70	11573.5
71	9163.0
72	7611.5
73	6060.0
74	5086.0
75	3076.5
76	2041.0
77	1698.0
78	1355.0
79	943.5
80	532.0
81	399.5
82	267.0
83	162.5
84	58.0
85	43.5
86	29.0
87	20.0
88	11.0
89	9.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1140977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.91153006500511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48422214169985	20.95629767688264
2	11.053613466073719	6.391537561026713
3	4.032160541087684	3.497277921317516
4	2.02770032844657	2.34495676034815
5	1.222981964929909	1.7679139924015042
6	0.8457372702162856	1.4670935108972094
7	0.6478986673565856	1.3112219260249671
8	0.49602631284863125	1.1472703725565465
9	0.43957800761812316	1.1437985504849768
>10	6.060140603022012	43.604059495063936
>50	0.634016270801959	11.035919532051729
>100	0.05014962105054549	2.8505078760646714
>500	0.0033433080699639527	0.7272358328566774
>1k	0.0024314967781556016	1.7549089920228012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3396	0.2976396544365048	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2868	0.25136352441810833	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	2636	0.23103007334941897	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2534	0.2220903664140469	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	2506	0.21963632921610166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	2392	0.20964489205303874	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1851	0.1622293876213105	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1788	0.15670780392593364	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.764418564090249E-5	0.0	0.0	0.16696217364591925	0.0
2	8.764418564090249E-5	0.0	0.0	0.8103581404357845	0.0
3	8.764418564090249E-5	0.0	0.0	1.156727962088631	0.0
4	8.764418564090249E-5	0.0	0.0	1.5938095158798118	0.0
5	8.764418564090249E-5	0.0	0.0	2.805928603293493	0.0
6	8.764418564090249E-5	0.0	0.0	3.58061556017343	0.0
7	8.764418564090249E-5	0.0	0.0	4.302803649854467	0.0
8	1.7528837128180498E-4	0.0	0.0	5.410012647055988	0.0
9	1.7528837128180498E-4	0.0	0.0	5.9057281610409325	0.0
10	1.7528837128180498E-4	0.0	0.0	7.052552330152142	0.0
11	1.7528837128180498E-4	0.0	0.0	8.329615759125732	0.0
12	1.7528837128180498E-4	0.0	0.0	9.531217544262505	0.0
13	1.7528837128180498E-4	0.0	0.0	9.962602225987027	0.0
14	1.7528837128180498E-4	0.0	0.0	10.178031634292365	0.0
15	2.6293255692270745E-4	0.0	0.0	10.499510507223196	0.0
16	2.6293255692270745E-4	0.0	0.0	11.100749620719787	0.0
17	2.6293255692270745E-4	0.0	0.0	11.782533740820368	0.0
18	2.6293255692270745E-4	0.0	0.0	12.61515350440894	0.0
19	2.6293255692270745E-4	0.0	0.0	13.108940846309785	0.0
20	2.6293255692270745E-4	0.0	0.0	13.608074483534725	0.0
21	2.6293255692270745E-4	0.0	0.0	14.205019031934912	0.0
22	2.6293255692270745E-4	0.0	0.0	14.82738039417096	0.0
23	2.6293255692270745E-4	0.0	0.0	15.421958549558843	0.0
24	3.5057674256360995E-4	0.0	0.0	15.917060554244301	0.0
25	3.5057674256360995E-4	0.0	0.0	16.36869104285187	0.0
26	3.5057674256360995E-4	0.0	0.0	16.80831427802664	0.0
27	3.5057674256360995E-4	0.0	0.0	17.34636193367614	0.0
28	3.5057674256360995E-4	0.0	0.0	17.83322538491135	0.0
29	3.5057674256360995E-4	0.0	0.0	18.35427006854652	0.0
30	4.3822092820451245E-4	0.0	0.0	18.974703258698465	0.0
31	4.3822092820451245E-4	0.0	0.0	19.44412551699114	0.0
32	4.3822092820451245E-4	0.0	0.0	19.911444314828433	0.0
33	4.3822092820451245E-4	0.0	0.0	20.409175645083117	0.0
34	4.3822092820451245E-4	0.0	0.0	20.96308689833362	0.0
35	5.258651138454149E-4	0.0	0.0	21.513229451601568	0.0
36	6.135092994863174E-4	0.0	0.0	21.99825237493832	0.0
37	6.135092994863174E-4	0.0	0.0	22.528762630622705	0.0
38	6.135092994863174E-4	0.0	0.0	23.165059418375655	0.0
39	6.135092994863174E-4	0.0	0.0	24.276913557416144	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTCGT	35	1.2119199E-7	45.000004	11
CGAACCT	25	3.8907885E-5	45.0	41
ACACGAT	20	7.033266E-4	45.0	32
TTGTACG	20	7.033266E-4	45.0	1
ACTACCG	20	7.033266E-4	45.0	1
TATTACG	20	7.033266E-4	45.0	1
ACGACTA	20	7.033266E-4	45.0	19
ATACGGA	130	0.0	41.53846	4
GCTAACG	55	6.184564E-11	40.909092	1
CGGTCTA	125	0.0	39.6	31
CGCATCG	40	3.45819E-7	39.375	21
TAGGACG	40	3.45819E-7	39.375	1
CGTTTTT	1655	0.0	39.018127	1
ACGGGAC	255	0.0	38.82353	5
TACGGAA	140	0.0	38.57143	5
ACGATAT	35	6.2482486E-6	38.57143	34
CCATACG	140	0.0	38.57143	2
CGACGGT	130	0.0	38.07692	28
AATCGTT	30	1.13991686E-4	37.499996	22
TAGCCGT	115	0.0	37.173916	44
>>END_MODULE