FastQCFastQC Report
Sat 14 Jan 2017
SRR2935761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371966
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.4789477290253549No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC49550.3611605535414143TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC41190.3002260988974945TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT28740.2094804098643844TruSeq Adapter, Index 21 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC25970.1892904051558129No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG19670.14337089986194992TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC18380.13396833449225418No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG18170.1324376843157921No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC15380.11210190339993849TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT14830.10809305769968061No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC14690.10707262424870588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA207.0337637E-445.00000427
GTATCGC207.0337637E-445.00000430
CGTTTTT35250.041.936171
TAGTACG603.6379788E-1241.2499961
TAACGCC501.0822987E-940.512
TTACCGG403.458881E-739.3750042
TTCGTTG356.2491763E-638.5714261
CGGTCTA356.2491763E-638.57142631
CTAGGCG356.2491763E-638.5714261
GCGATTT1650.038.1818169
CGTTAGG1550.037.7419362
GCGCGTG301.1400374E-437.4999963
CGTCTAT301.1400374E-437.49999620
TAGCCGT1200.037.49999644
GCCGATA301.1400374E-437.4999969
CTCGCGG601.5643309E-1037.4999962
TACGCGG1150.037.1739122
ATAACGC552.750312E-936.8181811
ATCGGCT552.750312E-936.8181818
TAAGGGA16550.036.4350434
Produced by FastQC (version 0.11.5)