##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935760.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436731
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13579299117232	33.0	31.0	34.0	30.0	34.0
2	32.18783892043813	33.0	31.0	34.0	30.0	34.0
3	32.19465717660439	34.0	31.0	34.0	30.0	34.0
4	35.85403043436802	37.0	35.0	37.0	35.0	37.0
5	34.90532396113121	37.0	35.0	37.0	33.0	37.0
6	35.38800165097015	37.0	35.0	37.0	33.0	37.0
7	35.9448498013894	37.0	35.0	37.0	35.0	37.0
8	35.93139773555384	37.0	35.0	37.0	35.0	37.0
9	37.807993980779976	39.0	38.0	39.0	35.0	39.0
10	37.41425082357101	39.0	37.0	39.0	34.0	39.0
11	37.36005278649935	39.0	37.0	39.0	35.0	39.0
12	37.244988797485405	39.0	37.0	39.0	34.0	39.0
13	37.16470445754981	39.0	37.0	39.0	34.0	39.0
14	38.18615175700949	40.0	38.0	41.0	33.0	41.0
15	38.31834490938109	40.0	38.0	41.0	33.0	41.0
16	38.386474573180365	40.0	38.0	41.0	34.0	41.0
17	38.36860135961429	40.0	37.0	41.0	34.0	41.0
18	38.330370124957284	40.0	37.0	41.0	34.0	41.0
19	38.224479739074326	40.0	37.0	41.0	34.0	41.0
20	38.176562627241985	40.0	37.0	41.0	34.0	41.0
21	38.12306827095678	40.0	37.0	41.0	34.0	41.0
22	38.17874884024915	40.0	37.0	41.0	34.0	41.0
23	38.180314895411875	40.0	37.0	41.0	34.0	41.0
24	38.14418565479551	40.0	36.0	41.0	34.0	41.0
25	38.009676132832105	40.0	36.0	41.0	34.0	41.0
26	38.020353844943834	40.0	36.0	41.0	34.0	41.0
27	38.03111856012016	40.0	36.0	41.0	34.0	41.0
28	37.92491426718015	40.0	36.0	41.0	34.0	41.0
29	37.873738368560296	40.0	36.0	41.0	34.0	41.0
30	37.7475686123568	40.0	36.0	41.0	33.0	41.0
31	37.682067833157355	40.0	36.0	41.0	33.0	41.0
32	37.58088118095872	40.0	36.0	41.0	33.0	41.0
33	37.42250915446245	40.0	36.0	41.0	33.0	41.0
34	37.280584187297414	40.0	35.0	41.0	33.0	41.0
35	37.192900410724064	40.0	35.0	41.0	32.0	41.0
36	37.11674349617291	40.0	35.0	41.0	32.0	41.0
37	37.06429735280996	40.0	35.0	41.0	32.0	41.0
38	36.94359834930825	40.0	35.0	41.0	32.0	41.0
39	36.86473807553397	39.0	35.0	41.0	31.0	41.0
40	36.72438125160521	39.0	35.0	41.0	31.0	41.0
41	36.63101304280342	39.0	35.0	41.0	31.0	41.0
42	36.65411548856397	39.0	35.0	41.0	31.0	41.0
43	36.57488005757515	39.0	35.0	41.0	31.0	41.0
44	36.47383609040245	39.0	35.0	41.0	31.0	41.0
45	36.44637374706887	39.0	35.0	41.0	31.0	41.0
46	36.33591535228237	39.0	35.0	41.0	30.0	41.0
47	36.22522657338082	39.0	35.0	41.0	30.0	41.0
48	36.15352978393311	39.0	35.0	41.0	30.0	41.0
49	36.14468261630048	39.0	35.0	41.0	30.0	41.0
50	36.012094122003354	38.0	35.0	40.0	30.0	41.0
51	34.829849846631	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	26.0
10	18.0
11	32.0
12	27.0
13	33.0
14	25.0
15	50.0
16	102.0
17	209.0
18	341.0
19	689.0
20	1264.0
21	2085.0
22	3066.0
23	4487.0
24	6316.0
25	9415.0
26	12171.0
27	13945.0
28	15553.0
29	16866.0
30	19944.0
31	25734.0
32	34277.0
33	50111.0
34	90001.0
35	104001.0
36	102308.0
37	153521.0
38	273222.0
39	496498.0
40	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35014696557671	20.402497057556353	23.718427457888776	12.528928518978152
2	33.308670864622535	25.507697683143192	24.41695766291672	16.766673789317554
3	30.91225845339176	25.061685172798526	27.77771204212897	16.248344331680737
4	27.407983818822036	24.048064669029902	29.641665698032547	18.902285814115515
5	25.162887137536533	30.098744998193816	26.23720097916729	18.50116688510236
6	24.865754271328452	34.758698740404434	25.987884997261148	14.38766199100597
7	78.23510455332278	5.553022799675096	11.627785577119168	4.5840870698829495
8	79.76614968285642	6.259487684194188	9.346147608703369	4.628215024246014
9	74.23024908629382	6.40091986600136	11.204045851311067	8.164785196393758
10	40.40512803022974	22.954401345833006	20.53745621135759	16.103014412579668
11	31.611902297646534	24.841184605886557	24.127202656586373	19.419710439880532
12	26.85756763096223	21.6604221667104	29.844000025056882	21.638010177270484
13	25.923293922105113	21.797956611223672	32.47170138320952	19.80704808346169
14	21.45342447542372	25.31086194980132	31.49921592838186	21.736497646393097
15	19.73090300132732	25.09961850896236	34.2871421302944	20.882336359415923
16	23.754690335212366	25.07247355280842	29.020533419269164	22.152302692710048
17	24.558737856982273	25.246827694258698	28.333696426122913	21.86073802263611
18	24.93382546906832	24.85531390357694	29.308061147145846	20.90279948020889
19	24.204948595109315	26.566629382953383	27.639690380453963	21.58873164148334
20	24.004841546538636	27.311584423249723	28.6963948018105	19.987179228401143
21	24.08822528364739	26.891255217573786	30.162083229219665	18.85843626955916
22	22.6196135532678	23.613884575470287	30.380704529936363	23.38579734132555
23	21.677126755112823	26.103285862141206	30.368802510699638	21.850784872046333
24	22.916259202314144	25.448814009024655	28.962067359860683	22.67285942880052
25	23.231488705958178	27.508280951688242	27.197157992693132	22.063072349660445
26	21.279835960941888	25.208337538481455	28.805531446039655	24.706295054537
27	21.658821310321834	25.29819430359615	30.103895579617895	22.93908880646412
28	19.81546998011458	25.63339971087142	30.511974753798725	24.039155555215277
29	21.289371496821605	25.208407140933133	30.636563142300123	22.86565821994514
30	22.721024325360837	25.03969079806867	31.188301776741785	21.050983099828706
31	23.54748383657066	24.976213362139468	29.300683287268107	22.175619514021765
32	24.21016877898507	25.63395653048483	29.16370566236825	20.992169028161847
33	23.409531777347325	25.3735041563104	28.01777089796211	23.199193168380162
34	22.005511122123767	25.079224990621068	30.254724092401432	22.660539794853733
35	21.227286109925934	26.576930545801545	29.721778119912496	22.47400522436002
36	24.25638480689844	26.725253370324715	27.948934073253795	21.069427749523047
37	22.098639202467268	26.35830924508485	29.78741323184368	21.755638320604202
38	23.216036961685937	27.411603146309226	27.283673840127342	22.08868605187749
39	22.650377836908927	24.797404663781876	28.564915770593103	23.987301728716094
40	22.792993260394603	25.603749066457116	29.32608818213013	22.27716949101815
41	21.845703893073928	25.108527622776982	27.632660532834606	25.41310795131448
42	21.785636977276887	25.31315883070665	30.023087133221182	22.87811705879528
43	23.282507303037242	23.674856323139124	29.61069260703639	23.431943766787242
44	22.789513137810765	23.85916361517918	29.088256604750644	24.263066642259407
45	21.559637816682457	23.806335354356523	28.82689939870442	25.807127430256603
46	23.097643191383774	24.888514273026754	28.14034081536488	23.873501720224592
47	20.64325193790626	25.03843795393849	31.571393670770657	22.74691643738459
48	21.412289426482758	25.698547605640858	29.235604994950343	23.653557972926038
49	22.649821017295512	23.572262309367588	30.7564185640875	23.0214981092494
50	21.544951699378657	23.985492064972497	29.886179110772996	24.583377124875845
51	20.72447799901304	24.018762036873987	28.45731038030084	26.79944958381214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	597.0
1	1142.5
2	1688.0
3	7206.0
4	12724.0
5	8268.5
6	3813.0
7	3783.5
8	3754.0
9	3768.5
10	3783.0
11	3836.0
12	3889.0
13	3841.5
14	3794.0
15	3602.0
16	3410.0
17	3516.5
18	3623.0
19	3740.5
20	3858.0
21	4008.0
22	4158.0
23	4730.0
24	5302.0
25	6695.5
26	8964.0
27	9839.0
28	12722.0
29	15605.0
30	18042.5
31	20480.0
32	23428.0
33	26376.0
34	30933.0
35	35490.0
36	38532.0
37	41574.0
38	47014.5
39	52455.0
40	57818.0
41	63181.0
42	72737.0
43	82293.0
44	108738.0
45	135183.0
46	135291.5
47	135400.0
48	137699.0
49	139998.0
50	124718.0
51	109438.0
52	100880.5
53	92323.0
54	86435.5
55	80548.0
56	77318.0
57	74088.0
58	68803.5
59	63519.0
60	58006.0
61	52493.0
62	47840.0
63	43187.0
64	37361.5
65	31536.0
66	27868.0
67	24200.0
68	20804.5
69	17409.0
70	14364.5
71	11320.0
72	9289.0
73	7258.0
74	5895.5
75	3451.0
76	2369.0
77	1687.5
78	1006.0
79	818.0
80	630.0
81	493.5
82	357.0
83	210.0
84	63.0
85	44.0
86	25.0
87	24.5
88	24.0
89	27.5
90	31.0
91	18.5
92	6.0
93	7.0
94	8.0
95	5.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1436731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.233238259772175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57644879967184	20.490681710092915
2	10.147693847489306	5.730045163667796
3	3.8744685781746404	3.2816638349281613
4	2.050852121521232	2.3160878632987276
5	1.2583506521315193	1.7763656887984443
6	0.9239489984503776	1.565164332787643
7	0.7187999856660461	1.4205835879501214
8	0.5755826429724412	1.3000449517784254
9	0.49361367032183073	1.2542681126229194
>10	6.876069756485478	46.580305674652564
>50	0.4491681410930182	7.582854271936909
>100	0.04743361262085118	2.381005836318135
>500	0.003027677360591803	0.5717975675353546
>1k	0.00353229025402377	1.6265635551391913
>5k	0.0010092257868639341	2.1225678484927073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	7906	0.5502769829564477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7765	0.5404630372700248	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	7339	0.510812392855726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	6787	0.4723918395301556	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	3847	0.2677606316004875	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2337	0.16266092956858313	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	1938	0.13488955134955674	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	1906	0.1326622728959005	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	1817	0.12646765469666904	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	1463	0.10182838680309675	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4801225838378933E-4	0.0	0.0	0.14226741122729308	0.0
2	3.4801225838378933E-4	0.0	0.0	0.7150955885270103	0.0
3	3.4801225838378933E-4	0.0	0.0	1.073896226920697	0.0
4	3.4801225838378933E-4	0.0	0.0	1.5803236653207873	0.0
5	3.4801225838378933E-4	0.0	0.0	3.0927153378050587	0.0
6	3.4801225838378933E-4	0.0	0.0	3.989751735015114	0.0
7	3.4801225838378933E-4	0.0	0.0	4.74507754061129	0.0
8	3.4801225838378933E-4	0.0	0.0	5.7787435504628215	0.0
9	3.4801225838378933E-4	0.0	0.0	6.203736120401105	0.0
10	3.4801225838378933E-4	0.0	0.0	7.524025026257525	0.0
11	3.4801225838378933E-4	0.0	0.0	8.623534955395268	0.0
12	3.4801225838378933E-4	0.0	0.0	10.012869493315032	0.0
13	3.4801225838378933E-4	0.0	0.0	10.404592091351827	0.0
14	3.4801225838378933E-4	0.0	0.0	10.589873817715356	0.0
15	4.1761471006054716E-4	0.0	0.0	10.911437144461976	0.0
16	4.1761471006054716E-4	0.0	0.0	11.432689905069216	0.0
17	4.1761471006054716E-4	0.0	0.0	12.037047992978504	0.0
18	4.8721716173730505E-4	0.0	0.0	12.710730122757845	0.0
19	4.8721716173730505E-4	0.0	0.0	13.22885077303963	0.0
20	4.8721716173730505E-4	0.0	0.0	13.704374722895238	0.0
21	4.8721716173730505E-4	0.0	0.0	14.257435803918757	0.0
22	4.8721716173730505E-4	0.0	0.0	14.883231446944487	0.0
23	4.8721716173730505E-4	0.0	0.0	15.45612922669588	0.0
24	4.8721716173730505E-4	0.0	0.0	15.926154582869026	0.0
25	4.8721716173730505E-4	0.0	0.0	16.35929063965349	0.0
26	5.568196134140629E-4	0.0	0.0	16.761662412796827	0.0
27	5.568196134140629E-4	0.0	0.0	17.244703427433528	0.0
28	6.264220650908207E-4	0.0	0.0	17.686400585774233	0.0
29	6.264220650908207E-4	0.0	0.0	18.18155242700269	0.0
30	6.264220650908207E-4	0.0	0.0	18.780899138391252	0.0
31	6.960245167675787E-4	0.0	0.0	19.26052963289579	0.0
32	6.960245167675787E-4	0.0	0.0	19.71566006441011	0.0
33	6.960245167675787E-4	0.0	0.0	20.180465236707498	0.0
34	6.960245167675787E-4	0.0	0.0	20.648959338943754	0.0
35	6.960245167675787E-4	0.0	0.0	21.128589833448295	0.0
36	6.960245167675787E-4	0.0	0.0	21.567154881463544	0.0
37	6.960245167675787E-4	0.0	0.0	22.042052409254065	0.0
38	6.960245167675787E-4	0.0	0.0	22.586413183817985	0.0
39	6.960245167675787E-4	0.0	0.0	23.406747679280254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGA	30	2.1658816E-6	45.000004	39
ACGTTAG	35	1.2122109E-7	45.0	1
ACCGCGT	20	7.033877E-4	45.0	21
TAGCGAT	25	3.891295E-5	45.0	1
CGTTTTT	3845	0.0	42.834854	1
CGCGTAG	45	1.9288564E-8	40.0	2
CACAACG	410	0.0	39.512196	12
CGTAAGG	305	0.0	38.360657	2
CGCATCG	85	0.0	37.058823	21
AGTACGG	165	0.0	36.818184	2
ACAACGA	435	0.0	36.72414	13
AGCGACT	430	0.0	36.104652	19
GCGCGTA	25	0.0021072836	36.0	1
CGTACGA	25	0.0021072836	36.0	27
TTAACGA	25	0.0021072836	36.0	15
TAATGCG	50	4.8803486E-8	36.0	1
TGTACGG	245	0.0	35.816326	2
CGAGATA	715	0.0	35.55944	19
CAACGAG	460	0.0	35.21739	14
TGGGCGA	495	0.0	35.0	6
>>END_MODULE