FastQCFastQC Report
Sat 14 Jan 2017
SRR2935759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759958
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.5012051423954434No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC23550.13381001137527146No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC22900.1301167414222385No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC20320.11545730068558455No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG19940.11329815825150372No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT18800.10682073094926128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG406.8193913E-945.01
TCGGTTA207.034311E-445.031
ACGATAG800.042.18751
TAGCCGT1400.041.78571344
CGTTTTT45450.041.2871321
CGGTCTA1100.040.90909231
CGTAAGG2150.040.8139532
CAACGTT403.459627E-739.3758
ACGTAAG1150.039.1304321
TTGATCG356.250182E-638.57142613
TCACGAC1250.037.80000325
CCAACGA301.14016824E-437.50000428
AAATGCG601.5643309E-1037.5000041
TACGGGA4550.036.09894
CCGTAAT250.00210741236.014
CTCGACA504.88144E-836.030
CGGGTAT3250.035.307696
TACGAAT2550.035.29411712
CGAATAT2550.035.29411714
TAAGGGA10150.035.2463074
Produced by FastQC (version 0.11.5)