##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809177
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94093257717409	33.0	31.0	34.0	30.0	34.0
2	31.976738093148963	33.0	31.0	34.0	30.0	34.0
3	32.025553123729416	33.0	31.0	34.0	30.0	34.0
4	35.71863016373426	37.0	35.0	37.0	33.0	37.0
5	34.76123023763651	37.0	35.0	37.0	33.0	37.0
6	35.22785620451397	37.0	35.0	37.0	33.0	37.0
7	35.777350320140094	37.0	35.0	37.0	35.0	37.0
8	35.737949793432094	37.0	35.0	37.0	35.0	37.0
9	37.55988615593375	39.0	37.0	39.0	35.0	39.0
10	37.27630543132096	39.0	37.0	39.0	34.0	39.0
11	37.2001675776746	39.0	37.0	39.0	34.0	39.0
12	37.03354148721479	39.0	37.0	39.0	33.0	39.0
13	36.88488674294994	39.0	35.0	39.0	33.0	39.0
14	37.878842329922875	40.0	37.0	41.0	33.0	41.0
15	37.95995560921776	40.0	37.0	41.0	33.0	41.0
16	38.06818903651488	40.0	37.0	41.0	33.0	41.0
17	38.01494728594609	40.0	36.0	41.0	33.0	41.0
18	38.00400901162539	40.0	36.0	41.0	33.0	41.0
19	37.943744075770816	40.0	36.0	41.0	33.0	41.0
20	37.878581571151926	40.0	36.0	41.0	33.0	41.0
21	37.828756872723766	40.0	36.0	41.0	33.0	41.0
22	37.8785098933855	40.0	36.0	41.0	33.0	41.0
23	37.874780177884446	40.0	36.0	41.0	33.0	41.0
24	37.82265561181299	40.0	36.0	41.0	33.0	41.0
25	37.6836563570146	40.0	35.0	41.0	33.0	41.0
26	37.675402291464046	40.0	35.0	41.0	33.0	41.0
27	37.66359152571069	40.0	36.0	41.0	33.0	41.0
28	37.530628033174445	40.0	35.0	41.0	33.0	41.0
29	37.489042570414135	40.0	35.0	41.0	33.0	41.0
30	37.39859758742525	39.0	35.0	41.0	33.0	41.0
31	37.342365143843686	39.0	35.0	41.0	33.0	41.0
32	37.25225630486284	39.0	35.0	41.0	32.0	41.0
33	37.13506315676298	39.0	35.0	41.0	32.0	41.0
34	37.02920745399338	39.0	35.0	41.0	32.0	41.0
35	36.95207723402914	39.0	35.0	41.0	31.0	41.0
36	36.911018232104965	39.0	35.0	41.0	32.0	41.0
37	36.85443852210332	39.0	35.0	41.0	31.0	41.0
38	36.7763975001761	39.0	35.0	41.0	31.0	41.0
39	36.69548813176845	39.0	35.0	41.0	31.0	41.0
40	36.57987807364767	39.0	35.0	41.0	31.0	41.0
41	36.473734423988816	39.0	35.0	41.0	31.0	41.0
42	36.5011091516442	39.0	35.0	41.0	31.0	41.0
43	36.41185179509551	39.0	35.0	41.0	31.0	41.0
44	36.30506428136242	38.0	35.0	40.0	30.0	41.0
45	36.26650658632166	38.0	35.0	40.0	30.0	41.0
46	36.14946544451956	38.0	35.0	40.0	30.0	41.0
47	36.01168100427966	38.0	35.0	40.0	30.0	41.0
48	35.976183208370976	38.0	35.0	40.0	30.0	41.0
49	35.98660243679689	38.0	35.0	40.0	30.0	41.0
50	35.86244542294207	38.0	35.0	40.0	30.0	41.0
51	34.710272289004756	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	11.0
11	14.0
12	14.0
13	15.0
14	27.0
15	32.0
16	45.0
17	100.0
18	204.0
19	446.0
20	790.0
21	1423.0
22	2247.0
23	3046.0
24	3926.0
25	4949.0
26	6093.0
27	7268.0
28	8301.0
29	9952.0
30	12695.0
31	16467.0
32	22353.0
33	31563.0
34	57369.0
35	70924.0
36	64086.0
37	92478.0
38	151390.0
39	240794.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08309059698929	17.59429642711051	21.106877728852897	16.21573524704731
2	28.648861744710985	28.849188743624694	25.000957763258224	17.500991748406097
3	29.74528440625475	28.632301709020403	25.632340019550732	15.99007386517412
4	27.69703043957008	24.42864787308586	29.209678475784656	18.664643211559397
5	27.18663530970356	29.83203922009647	23.91133213128895	19.06999333891102
6	24.77282473426704	35.70590859601793	24.45225210306274	15.069014566652289
7	75.65155707589317	6.143031747071407	13.181911992061067	5.023499184974363
8	75.55924105603596	8.225518026340344	9.855940047727506	6.359300869896203
9	69.40310957923916	7.11686071156249	12.069052877182619	11.41097683201574
10	42.369716390851444	22.085155658156374	18.718525118731748	16.82660283226043
11	32.46026518301929	22.287089227696782	25.17261365560316	20.080031933680765
12	28.317043119119795	18.96037578922782	30.015929765675498	22.706651325976885
13	26.04399284705324	20.947580072097946	33.457821959843145	19.550605121005663
14	21.046569539173753	27.202948180682345	29.929174951833776	21.821307328310123
15	18.297850779248545	23.268951045321355	37.39911045420223	21.034087721227866
16	21.97195422015208	22.799708839969497	30.217863335215906	25.010473604662515
17	22.41882801908606	23.33951657054019	28.586452654981542	25.65520275539221
18	23.423428990196214	23.084195423251032	30.124311491799695	23.368064094753063
19	23.92183663154044	25.135786113544995	26.709113086506413	24.233264168408148
20	24.460655703263935	25.74665369875812	28.05714942466234	21.735541173315603
21	24.11474868910016	27.786380482885697	28.14612872091026	19.952742107103884
22	21.703162596069834	22.77041982162123	29.158391798086203	26.368025784222738
23	21.70897096679713	26.161396085158128	27.876348437980813	24.25328451006393
24	23.164894701653655	23.865977406673693	27.446776168872816	25.52235172279983
25	22.499403715132782	27.142516408647303	25.42620464990972	24.93187522631019
26	20.65580212981832	23.97794302111899	27.65649542683492	27.70975942222777
27	22.933424949053176	24.757624104491356	27.605826660915966	24.703124285539506
28	20.82585145153656	24.076190994059395	29.236990176438532	25.860967377965515
29	22.115309752995945	23.696669579090855	26.56254441240915	27.62547625550405
30	22.888935300929216	23.95557461470111	28.724988475945313	24.43050160842436
31	26.142611567061348	23.018820356979994	25.00169925739362	25.836868818565033
32	25.967001039327613	25.719959909883745	24.864152095277053	23.44888695551159
33	24.05209243465892	22.162023883526103	25.912254055663965	27.873629626151015
34	22.937626749153768	21.64483172408509	26.736795534227987	28.68074599253315
35	20.493414914165875	23.536383263488705	29.456225275804922	26.513976546540498
36	23.842002429629115	23.026235298333987	28.13216391469357	24.999598357343324
37	21.242323990919168	24.944974956035576	30.563646767023776	23.249054286021476
38	23.282050775046745	24.908518161045112	26.21354783934788	25.59588322456026
39	22.18562811350298	23.89477209559837	27.375963478942182	26.54363631195647
40	22.207996519920858	20.942142448438354	30.21267287626811	26.637188155372684
41	19.753403767037373	21.839721099339204	29.656057945295032	28.750817188328387
42	21.341437040350876	21.7383835675013	32.1364794105616	24.78369998158623
43	22.85297283536235	21.12492075281428	30.483936147468356	25.53817026435502
44	23.005967791966405	21.77236871537377	28.539738524451387	26.68192496820844
45	21.28236467423073	21.67325566594206	27.91984942725757	29.124530232569633
46	24.767139945895643	22.604943047071284	27.467661587019897	25.160255420013172
47	20.59153930475038	21.691298689903444	32.584094703630974	25.1330673017152
48	21.43115783073419	22.74669200928845	29.13145084450003	26.690699315477328
49	22.170674648439093	19.932721765448104	32.71546274795255	25.181140838160253
50	21.841451252321804	20.172224371182075	31.20812875304167	26.778195623454447
51	20.757016079300325	20.97785774929342	27.895009373721695	30.37011679768456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	225.0
1	625.5
2	1026.0
3	1869.0
4	2712.0
5	1927.5
6	1143.0
7	1163.0
8	1183.0
9	1248.0
10	1313.0
11	1302.5
12	1292.0
13	1354.5
14	1417.0
15	1414.0
16	1411.0
17	1467.5
18	1524.0
19	1509.0
20	1494.0
21	1786.5
22	2079.0
23	2023.5
24	1968.0
25	2422.5
26	3560.0
27	4243.0
28	5194.5
29	6146.0
30	6851.5
31	7557.0
32	9078.5
33	10600.0
34	12326.0
35	14052.0
36	15744.0
37	17436.0
38	19394.5
39	21353.0
40	25631.5
41	29910.0
42	32669.5
43	35429.0
44	43778.5
45	52128.0
46	75527.5
47	98927.0
48	91663.5
49	84400.0
50	83398.0
51	82396.0
52	71320.0
53	60244.0
54	54177.0
55	48110.0
56	45031.0
57	41952.0
58	40036.0
59	38120.0
60	35452.5
61	32785.0
62	30429.5
63	28074.0
64	24611.0
65	21148.0
66	18951.0
67	16754.0
68	14700.5
69	12647.0
70	10973.5
71	9300.0
72	7592.0
73	5884.0
74	5023.0
75	3148.0
76	2134.0
77	1513.5
78	893.0
79	648.0
80	403.0
81	304.5
82	206.0
83	139.5
84	73.0
85	49.0
86	25.0
87	19.5
88	14.0
89	8.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	809177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.20703154549053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16575054913025	22.101201348870685
2	10.709639225623395	6.4701281985845736
3	3.943990584914174	3.5740874404086034
4	1.9114280488884607	2.309542694788366
5	1.168482348876842	1.7648191586435817
6	0.8269635187761072	1.498806785918383
7	0.6318307818034413	1.3360012650143946
8	0.5048876065937871	1.220092468744459
9	0.43041542749259293	1.1701415156341093
>10	6.411625058674984	45.471542417154346
>50	0.23730785653668268	4.3734499066198325
>100	0.05026312220062777	2.85676803476613
>500	0.002471956829539071	0.49357185911054113
>1k	0.003707935244308606	2.13968254505485
>5k	0.0012359784147695355	3.2201643606871535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	9315	1.1511696452074145	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	8964	1.1077922382865555	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	7596	0.9387315754155148	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	4143	0.5120017004932172	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2650	0.3274932431346912	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	1996	0.2466703823761674	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1719	0.2124380697918997	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1537	0.18994608101812088	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1361	0.16819558637974139	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	1348	0.16658901575304289	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	1232	0.15225346246865643	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	1207	0.14916390357115933	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	852	0.1052921672267007	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.943294235995339E-4	0.0	0.0	0.20094491069321052	0.0
2	4.943294235995339E-4	0.0	0.0	1.2827848542407903	0.0
3	4.943294235995339E-4	0.0	0.0	1.7025941172326944	0.0
4	4.943294235995339E-4	0.0	0.0	2.4470542291735926	0.0
5	4.943294235995339E-4	0.0	0.0	4.90918550576697	0.0
6	4.943294235995339E-4	0.0	0.0	6.3626375935055	0.0
7	4.943294235995339E-4	0.0	0.0	7.605011017367029	0.0
8	4.943294235995339E-4	0.0	0.0	9.470733844387569	0.0
9	4.943294235995339E-4	0.0	0.0	10.236326539187347	0.0
10	4.943294235995339E-4	0.0	0.0	12.499490222781914	0.0
11	4.943294235995339E-4	0.0	0.0	14.052796854087548	0.0
12	6.179117794994173E-4	0.0	0.0	16.52246665439082	0.0
13	6.179117794994173E-4	0.0	0.0	17.16657789334101	0.0
14	6.179117794994173E-4	0.0	0.0	17.441177888150552	0.0
15	6.179117794994173E-4	0.0	0.0	17.967267977216355	0.0
16	6.179117794994173E-4	0.0	0.0	18.69615671231387	0.0
17	6.179117794994173E-4	0.0	2.4716471179976694E-4	19.484241395887427	0.0
18	7.414941353993008E-4	0.0	2.4716471179976694E-4	20.430140747945135	0.0
19	7.414941353993008E-4	0.0	2.4716471179976694E-4	21.127515982288177	0.0
20	7.414941353993008E-4	0.0	2.4716471179976694E-4	21.723800849505114	0.0
21	7.414941353993008E-4	0.0	2.4716471179976694E-4	22.33429768765054	0.0
22	7.414941353993008E-4	0.0	2.4716471179976694E-4	23.02079767467439	0.0
23	7.414941353993008E-4	0.0	2.4716471179976694E-4	23.627092712719218	0.0
24	8.650764912991843E-4	0.0	2.4716471179976694E-4	24.12537677170755	0.0
25	8.650764912991843E-4	0.0	2.4716471179976694E-4	24.569655341167632	0.0
26	8.650764912991843E-4	0.0	2.4716471179976694E-4	24.997868204360728	0.0
27	8.650764912991843E-4	0.0	2.4716471179976694E-4	25.50196063407635	0.0
28	8.650764912991843E-4	0.0	2.4716471179976694E-4	25.920163326441557	0.0
29	8.650764912991843E-4	0.0	2.4716471179976694E-4	26.390641355352415	0.0
30	8.650764912991843E-4	0.0	2.4716471179976694E-4	27.00014953465064	0.0
31	0.0011122412030989512	0.0	2.4716471179976694E-4	27.443563027619422	0.0
32	0.0011122412030989512	0.0	2.4716471179976694E-4	27.86955140840632	0.0
33	0.0011122412030989512	0.0	2.4716471179976694E-4	28.299618006937912	0.0
34	0.0011122412030989512	0.0	2.4716471179976694E-4	28.740930599856398	0.0
35	0.0011122412030989512	0.0	2.4716471179976694E-4	29.233653452829234	0.0
36	0.0011122412030989512	0.0	2.4716471179976694E-4	29.657170186498135	0.0
37	0.0011122412030989512	0.0	2.4716471179976694E-4	30.105650556058812	0.0
38	0.0011122412030989512	0.0	2.4716471179976694E-4	30.57229753193677	0.0
39	0.0011122412030989512	0.0	2.4716471179976694E-4	31.174020022813302	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGA	20	7.0320466E-4	45.000004	43
ACGATTG	20	7.0320466E-4	45.000004	1
ATACCGG	20	7.0320466E-4	45.000004	2
GTTTACG	25	3.8897786E-5	45.0	1
TCGAATA	25	3.8897786E-5	45.0	25
GTACGAG	45	3.8380676E-10	45.0	1
CTCACGA	60	3.6379788E-12	41.249996	24
TGGCGTA	55	6.002665E-11	40.909092	13
CGTAAGG	110	0.0	40.909092	2
ATAACGG	55	6.002665E-11	40.909092	2
ACAAGCG	105	0.0	40.714287	14
ACATACG	90	0.0	40.0	17
CGTTGTC	40	3.4565346E-7	39.375004	24
ACGTCAT	40	3.4565346E-7	39.375004	35
TAACGGG	150	0.0	39.0	3
GCGATTA	75	0.0	39.0	9
TACGCGC	35	6.2459985E-6	38.571426	34
TCATCGG	35	6.2459985E-6	38.571426	44
TCACGAC	65	9.094947E-12	38.076927	25
GCGTTAG	65	9.094947E-12	38.076927	1
>>END_MODULE