##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935755.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821712
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97214474170999	33.0	31.0	34.0	30.0	34.0
2	31.989660611016998	33.0	31.0	34.0	30.0	34.0
3	32.04067970286427	33.0	31.0	34.0	30.0	34.0
4	35.721100580251964	37.0	35.0	37.0	33.0	37.0
5	34.870721128570594	37.0	35.0	37.0	33.0	37.0
6	35.32781558502249	37.0	35.0	37.0	33.0	37.0
7	35.910359103919625	37.0	35.0	37.0	35.0	37.0
8	35.91935860739529	37.0	35.0	37.0	35.0	37.0
9	37.747393247269116	39.0	38.0	39.0	35.0	39.0
10	37.27299710847596	39.0	37.0	39.0	34.0	39.0
11	37.23730455439375	39.0	37.0	39.0	34.0	39.0
12	37.29249542418755	39.0	37.0	39.0	34.0	39.0
13	37.266374350137276	39.0	37.0	39.0	34.0	39.0
14	38.39542078002999	40.0	38.0	41.0	34.0	41.0
15	38.48226872675585	40.0	38.0	41.0	34.0	41.0
16	38.47504356757599	40.0	38.0	41.0	34.0	41.0
17	38.465152024066825	40.0	38.0	41.0	34.0	41.0
18	38.46269739276048	40.0	38.0	41.0	34.0	41.0
19	38.38028287283136	40.0	37.0	41.0	34.0	41.0
20	38.38401410713242	40.0	37.0	41.0	34.0	41.0
21	38.299786299822806	40.0	37.0	41.0	34.0	41.0
22	38.358071927877404	40.0	37.0	41.0	34.0	41.0
23	38.32614103238117	40.0	37.0	41.0	34.0	41.0
24	38.31662796697626	40.0	37.0	41.0	34.0	41.0
25	38.17297179547092	40.0	37.0	41.0	34.0	41.0
26	38.22103486379656	40.0	37.0	41.0	34.0	41.0
27	38.24891811242868	40.0	37.0	41.0	34.0	41.0
28	38.15721201588878	40.0	37.0	41.0	34.0	41.0
29	38.12645793173277	40.0	37.0	41.0	34.0	41.0
30	37.986932161146484	40.0	37.0	41.0	34.0	41.0
31	37.96785856845221	40.0	37.0	41.0	34.0	41.0
32	37.91672751523648	40.0	37.0	41.0	34.0	41.0
33	37.738393743793445	40.0	37.0	41.0	33.0	41.0
34	37.68668097825029	40.0	37.0	41.0	33.0	41.0
35	37.64374622738867	40.0	37.0	41.0	33.0	41.0
36	37.512487343497476	40.0	36.0	41.0	33.0	41.0
37	37.50280998695407	40.0	36.0	41.0	33.0	41.0
38	37.419984130692995	40.0	36.0	41.0	33.0	41.0
39	37.33539975076426	40.0	36.0	41.0	33.0	41.0
40	37.24749182195222	40.0	36.0	41.0	32.0	41.0
41	37.195557567614934	39.0	36.0	41.0	32.0	41.0
42	37.214740687734874	39.0	36.0	41.0	32.0	41.0
43	37.12257335903577	39.0	36.0	41.0	32.0	41.0
44	37.012178232762814	39.0	35.0	41.0	31.0	41.0
45	37.023219814241486	39.0	35.0	41.0	31.0	41.0
46	36.87779416632591	39.0	35.0	41.0	31.0	41.0
47	36.782407948283584	39.0	35.0	41.0	31.0	41.0
48	36.727728206476236	39.0	35.0	41.0	31.0	41.0
49	36.7233276379072	39.0	35.0	41.0	31.0	41.0
50	36.579829672683374	39.0	35.0	41.0	31.0	41.0
51	35.286394746577876	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	16.0
10	11.0
11	9.0
12	8.0
13	8.0
14	5.0
15	14.0
16	21.0
17	47.0
18	113.0
19	244.0
20	464.0
21	831.0
22	1231.0
23	1861.0
24	2822.0
25	4000.0
26	5152.0
27	5973.0
28	7108.0
29	8456.0
30	10631.0
31	13973.0
32	18870.0
33	26017.0
34	43306.0
35	59800.0
36	59258.0
37	90869.0
38	172266.0
39	288130.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.87640925287692	20.109113655392644	23.89085226940826	18.123624822322178
2	27.569513406156904	26.86427848199856	28.66442257141188	16.901785540432655
3	29.8766477792706	25.63927507447865	28.658459411569993	15.825617734680764
4	27.804753977062525	26.018483361567068	28.53384154058843	17.642921120781978
5	24.251684288412484	31.42646085246412	26.630498276768506	17.69135658235489
6	24.76780185758514	34.740395661740365	25.587310395856456	14.904492084818038
7	79.45058609342446	5.471503397784138	10.237163385711783	4.840747123079619
8	81.43388437798158	4.5840878555990425	8.069128843195669	5.912898923223708
9	74.86272562649687	6.741291352688046	11.252969410206983	7.143013610608097
10	35.275254590416104	28.587996767723972	19.94397063691415	16.192778004945772
11	25.415839126117177	26.19603948828787	28.38366727028448	20.004454115310473
12	22.3605107385556	22.66037249060498	33.2760139805674	21.703102790272016
13	22.050548123916897	25.32188893432249	35.225237066028	17.402325875732615
14	20.57703915727165	28.60515606441186	30.41503592499562	20.40276885332087
15	18.176319683782154	26.900057441049906	35.85708861498919	19.06653426017875
16	20.02562941760617	26.45026481297584	31.763805323519676	21.76030044589832
17	20.641782035555035	27.19541639893296	29.19380512880425	22.96899643670775
18	21.509117549701113	25.43616254843546	31.8102936308585	21.244426271004926
19	20.678899663142317	28.47968631345289	28.773098117101853	22.06831590630294
20	21.183699398329342	27.412402398894013	30.31061977919271	21.093278423583932
21	20.810819362501704	29.605263157894733	30.052013357478046	19.531904122125514
22	19.476897015012558	26.407062328407033	29.440606927974766	24.675433728605643
23	17.950814884046963	28.260996553537005	29.83514905465662	23.95303950775941
24	19.91950951184843	25.95374048328368	30.904136729170318	23.22261327569757
25	17.947285666997683	29.65394201374691	28.28253694725159	24.116235372003818
26	17.71691298167728	29.711017972233584	29.99968358743696	22.57238545865218
27	19.66078139299414	28.494898455906693	29.273151663843294	22.571168487255875
28	18.034299121833442	27.15343088576046	31.525157232704405	23.287112759701696
29	20.290564090581615	26.350959947037406	29.358826450143116	23.999649512237863
30	19.9753684989388	27.583751971493665	30.648451038806783	21.792428490760756
31	21.461290573826354	26.823144848803476	27.53482972136223	24.180734856007945
32	22.825272114804214	27.72382537920829	27.11862550382616	22.33227700216134
33	20.84635492727379	25.30180890628347	30.49474755145355	23.357088614989195
34	21.2399234768386	25.120480168234128	29.46470296162159	24.174893393305684
35	22.4024962517281	25.431172965710612	29.55415035924996	22.612180423311333
36	22.34347313900734	25.357789590513462	31.208501275386023	21.09023599509317
37	22.51336234593142	24.939394824464046	31.54426368362638	21.002979145978156
38	21.702981093132387	25.548489008314352	29.818841637946143	22.929688260607122
39	23.720232879646396	23.53391455887221	31.348087894542125	21.397764666939267
40	24.368148451038806	23.25157719492961	31.10165118679051	21.278623167241076
41	21.734257258017408	24.879154740346983	29.69604922405904	23.690538777576574
42	22.468334404268163	24.16199349650486	30.742766322020366	22.626905777206613
43	21.82504332418171	23.838279105087913	31.890370348735324	22.446307221995056
44	20.875683937924723	24.751981229433184	30.365383492026403	24.00695134061569
45	21.869341083007185	23.442763401289017	29.242362287516794	25.445533228187006
46	24.07169421889908	24.464289191346847	29.722944291917365	21.741072297836713
47	21.35212823957786	24.10053644099149	31.960832992581345	22.58650232684931
48	21.3391066456374	23.197421967794067	30.73230036801215	24.73117101855638
49	22.673150690266176	21.195625718013122	32.83486184940709	23.29636174231361
50	21.849747843526686	21.53759468037463	32.13279105087914	24.47986642521954
51	21.283977841384814	21.203779426368364	29.38779036937516	28.124452362871665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	516.0
2	839.0
3	1673.5
4	2508.0
5	1815.5
6	1123.0
7	1168.0
8	1213.0
9	1268.0
10	1323.0
11	1363.0
12	1403.0
13	1379.0
14	1355.0
15	1437.0
16	1519.0
17	1593.0
18	1667.0
19	1538.0
20	1409.0
21	1702.0
22	1995.0
23	2667.5
24	3340.0
25	4498.0
26	6010.0
27	6364.0
28	7531.5
29	8699.0
30	10478.5
31	12258.0
32	15413.0
33	18568.0
34	20479.0
35	22390.0
36	24651.0
37	26912.0
38	29813.5
39	32715.0
40	37492.5
41	42270.0
42	48257.5
43	54245.0
44	61743.0
45	69241.0
46	84788.5
47	100336.0
48	104651.0
49	108966.0
50	105024.5
51	101083.0
52	82526.5
53	63970.0
54	53599.5
55	43229.0
56	36117.5
57	29006.0
58	24337.0
59	19668.0
60	16812.5
61	13957.0
62	11540.5
63	9124.0
64	7382.5
65	5641.0
66	4448.0
67	3255.0
68	2503.5
69	1752.0
70	1441.5
71	1131.0
72	820.0
73	509.0
74	446.5
75	282.5
76	181.0
77	205.0
78	229.0
79	126.0
80	23.0
81	18.5
82	14.0
83	26.0
84	38.0
85	21.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.66583475786441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22507314716566	25.759675242414804
2	11.493823251139542	8.198736016224855
3	4.408094196911304	4.716550776724189
4	2.3971423576950075	3.4198433288251056
5	1.441867858436383	2.5712710390833933
6	0.9886156732473913	2.11558819446458
7	0.7681597840541615	1.9177941947998771
8	0.5899114910060901	1.6831748607990897
9	0.5169643434867223	1.6594168365454762
>10	4.900096072457079	36.36207580143999
>50	0.2138781971741476	4.811092381140523
>100	0.05056543533047298	3.295820337397087
>500	0.0030749251214471736	0.7841388646115383
>1k	0.002391608427792246	2.0751957831133643
>5k	3.416583468274637E-4	0.6296263424161335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	5167	0.6288091204704325	No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	3458	0.42082870884202733	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	3145	0.38273750413770274	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGC	3047	0.3708111844539206	No Hit
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT	2477	0.3014438148645754	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCG	2414	0.2937768950678583	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.15771949296103743	No Hit
TCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	1193	0.14518468757910313	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2169713963043013E-4	0.0	0.0	0.31616916875985746	0.0
2	1.2169713963043013E-4	0.0	0.0	1.697310006425609	0.0
3	1.2169713963043013E-4	0.0	0.0	2.2816996709309345	0.0
4	1.2169713963043013E-4	0.0	0.0	3.0380473937340575	0.0
5	1.2169713963043013E-4	0.0	0.0	5.007107112954417	0.0
6	1.2169713963043013E-4	0.0	0.0	6.484023599509317	0.0
7	1.2169713963043013E-4	0.0	0.0	7.8521428432346125	0.0
8	1.2169713963043013E-4	0.0	0.0	10.087719298245615	0.0
9	1.2169713963043013E-4	0.0	0.0	11.007262885293144	0.0
10	1.2169713963043013E-4	0.0	0.0	13.0546955624355	0.0
11	1.2169713963043013E-4	0.0	0.0	15.049555075257512	0.0
12	1.2169713963043013E-4	0.0	0.0	17.27527406195845	0.0
13	1.2169713963043013E-4	0.0	0.0	18.164758455517262	0.0
14	1.2169713963043013E-4	0.0	1.2169713963043013E-4	18.50112934945577	0.0
15	2.4339427926086025E-4	0.0	1.2169713963043013E-4	19.088804836731118	0.0
16	2.4339427926086025E-4	0.0	1.2169713963043013E-4	20.08270537609284	0.0
17	2.4339427926086025E-4	0.0	1.2169713963043013E-4	21.365514924937205	0.0
18	2.4339427926086025E-4	0.0	1.2169713963043013E-4	22.85813034250443	0.0
19	2.4339427926086025E-4	0.0	1.2169713963043013E-4	23.72388379383531	0.0
20	2.4339427926086025E-4	0.0	1.2169713963043013E-4	24.554953560371516	0.0
21	2.4339427926086025E-4	0.0	1.2169713963043013E-4	25.528043888856438	0.0
22	2.4339427926086025E-4	0.0	1.2169713963043013E-4	26.56964970695329	0.0
23	2.4339427926086025E-4	0.0	1.2169713963043013E-4	27.604562182370465	0.0
24	2.4339427926086025E-4	0.0	1.2169713963043013E-4	28.37502677337072	0.0
25	2.4339427926086025E-4	0.0	1.2169713963043013E-4	29.069430652101953	0.0
26	2.4339427926086025E-4	0.0	1.2169713963043013E-4	29.72464805187219	0.0
27	2.4339427926086025E-4	0.0	1.2169713963043013E-4	30.407369005198902	0.0
28	2.4339427926086025E-4	0.0	1.2169713963043013E-4	31.08376170726483	0.0
29	2.4339427926086025E-4	0.0	1.2169713963043013E-4	31.794959791265065	0.0
30	2.4339427926086025E-4	0.0	1.2169713963043013E-4	32.65742741982592	0.0
31	2.4339427926086025E-4	0.0	1.2169713963043013E-4	33.3807952177892	0.0
32	2.4339427926086025E-4	0.0	1.2169713963043013E-4	34.068992542399286	0.0
33	2.4339427926086025E-4	0.0	1.2169713963043013E-4	34.712040228206476	0.0
34	2.4339427926086025E-4	0.0	1.2169713963043013E-4	35.35216718266254	0.0
35	2.4339427926086025E-4	0.0	1.2169713963043013E-4	36.002151605428665	0.0
36	2.4339427926086025E-4	0.0	1.2169713963043013E-4	36.63960122281286	0.0
37	2.4339427926086025E-4	0.0	1.2169713963043013E-4	37.277781023034834	0.0
38	2.4339427926086025E-4	0.0	1.2169713963043013E-4	37.914622154720874	0.0
39	3.6509141889129037E-4	0.0	1.2169713963043013E-4	38.57276028584224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.0321123E-4	45.0	23
CTCGTAG	35	1.211356E-7	45.0	1
CTATCGG	20	7.0321123E-4	45.0	2
TTTCGCG	35	1.211356E-7	45.0	1
CACGATA	25	3.889831E-5	45.0	11
CGCGATC	20	7.0321123E-4	45.0	25
TGCGGAC	20	7.0321123E-4	45.0	31
TTATCGG	35	1.211356E-7	45.0	2
AATGCGT	25	3.889831E-5	45.0	4
TCTCGAG	20	7.0321123E-4	45.0	1
CATACCG	20	7.0321123E-4	45.0	1
CGTGTCG	20	7.0321123E-4	45.0	2
TAGATCG	20	7.0321123E-4	45.0	1
AGTTACG	70	0.0	45.0	1
ACGTATA	25	3.889831E-5	45.0	38
ATACGTT	20	7.0321123E-4	45.0	30
TTACCGC	20	7.0321123E-4	45.0	27
TCGACCG	20	7.0321123E-4	45.0	17
TGTCGCG	20	7.0321123E-4	45.0	22
TCGCGGA	20	7.0321123E-4	45.0	3
>>END_MODULE