SRR2935754.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622709
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	8126	1.304943400528979	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	7405	1.189158981161345	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	6878	1.1045287606249468	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	3316	0.5325119758988549	Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	1757	0.2821542646725838	Illumina Single End Adapter 1 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	1580	0.2537300729554254	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	1515	0.24329181046042372	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	1199	0.19254579586933865	Illumina Single End Adapter 1 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	1173	0.18837049087133798	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	1149	0.18451636318087583	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	1112	0.17857458299141332	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.15031097992802417	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	55	1.8189894E-12	45.000004	2
GCGACAT	20	7.0307933E-4	45.0	30
GTCTGCG	25	3.88874E-5	45.0	1
CGGCCGA	20	7.0307933E-4	45.0	6
AACGGAC	20	7.0307933E-4	45.0	25
TCGGACC	20	7.0307933E-4	45.0	23
ACGGATT	20	7.0307933E-4	45.0	27
ACTCGAA	50	2.1827873E-11	45.0	42
CGTAACG	25	3.88874E-5	45.0	2
CGATCCA	20	7.0307933E-4	45.0	20
TATCGGA	20	7.0307933E-4	45.0	20
TCGAACG	20	7.0307933E-4	45.0	1
CGAAGAG	20	7.0307933E-4	45.0	35
CCGTGCG	30	2.1638934E-6	44.999996	1
ACTCGAT	30	2.1638934E-6	44.999996	13
TTCGGAC	30	2.1638934E-6	44.999996	22
GCCTCCG	75	0.0	42.0	44
CGAATGC	60	3.6379788E-12	41.249996	45
CGGCTAC	60	3.6379788E-12	41.249996	25
GCCGGAG	55	6.002665E-11	40.909092	1