SRR2935753.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935753.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333706
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	13862	4.153955877329146	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	12435	3.7263339586342465	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	12193	3.653815034791104	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3703	1.109659400789917	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	2769	0.8297723145523305	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	2284	0.6844348018914853	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT	2092	0.6268991267762641	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA	1960	0.5873433501345495	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC	1766	0.5292083450702115	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1606	0.4812619491408605	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	987	0.29576932988918386	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.2250483958933912	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT	700	0.20976548219091054	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATG	643	0.19268457864107927	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTAT	530	0.15882243651597514	No Hit
ATGAAGGGGTTCCAGAGGTCATCAAAACCTTTATTTGTCTCTCCTTTGAGC	506	0.1516304771265725	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGT	465	0.1393442131696763	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	429	0.12855627408557233	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTCTCCT	406	0.12166397967072813	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	85	0.0	45.000004	2
CGGGCAT	45	3.8380676E-10	45.000004	6
TCACGAC	25	3.884836E-5	45.0	12
ATTTACG	20	7.026085E-4	45.0	10
TTAGGCG	25	3.884836E-5	45.0	1
AGCCGGT	20	7.026085E-4	45.0	15
GATACCA	20	7.026085E-4	45.0	11
GTACCGG	35	1.2084638E-7	45.0	2
GGTACCG	25	3.884836E-5	45.0	1
TTTACGT	25	3.884836E-5	45.0	18
GCGAACC	50	2.1827873E-11	45.0	33
GTTTACG	25	3.884836E-5	45.0	17
CGCATGG	25	3.884836E-5	45.0	2
CTGAGCG	20	7.026085E-4	45.0	1
CCGGATT	20	7.026085E-4	45.0	42
TGAACAC	20	7.026085E-4	45.0	17
AATGGTC	40	6.7902874E-9	45.0	10
TTCCGTC	20	7.026085E-4	45.0	42
GAATATG	20	7.026085E-4	45.0	1
TCGATCA	20	7.026085E-4	45.0	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content