##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935753.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333706
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793419956488645	31.0	31.0	34.0	30.0	34.0
2	31.83761155028678	33.0	31.0	34.0	30.0	34.0
3	31.919153386513877	33.0	31.0	34.0	30.0	34.0
4	35.60157443977633	37.0	35.0	37.0	33.0	37.0
5	34.622527014797456	37.0	35.0	37.0	32.0	37.0
6	35.06000791115533	37.0	35.0	37.0	32.0	37.0
7	35.54456018171684	37.0	35.0	37.0	33.0	37.0
8	35.3696996757625	37.0	35.0	37.0	33.0	37.0
9	37.2540859319281	39.0	37.0	39.0	34.0	39.0
10	37.05374191653731	39.0	37.0	39.0	33.0	39.0
11	36.95767232234362	39.0	37.0	39.0	33.0	39.0
12	36.77371998106118	39.0	35.0	39.0	33.0	39.0
13	36.45562860721713	39.0	35.0	39.0	32.0	39.0
14	37.571500062929644	40.0	36.0	41.0	32.0	41.0
15	37.46734251107262	40.0	36.0	41.0	32.0	41.0
16	37.82223274379244	40.0	36.0	41.0	33.0	41.0
17	37.55457498516658	39.0	36.0	41.0	32.0	41.0
18	37.64012334210353	39.0	36.0	41.0	33.0	41.0
19	37.54444930567625	39.0	36.0	41.0	32.0	41.0
20	37.19712861021378	39.0	35.0	41.0	32.0	41.0
21	37.5264334474058	39.0	35.0	41.0	33.0	41.0
22	37.55192894344123	39.0	35.0	41.0	33.0	41.0
23	37.41823641169173	39.0	35.0	41.0	33.0	41.0
24	37.43714227493662	39.0	35.0	41.0	32.0	41.0
25	37.48167548680575	39.0	35.0	41.0	33.0	41.0
26	37.256096683907394	39.0	35.0	41.0	32.0	41.0
27	37.18885186361648	39.0	35.0	41.0	32.0	41.0
28	37.05165325166464	39.0	35.0	41.0	32.0	41.0
29	36.935311321942066	39.0	35.0	41.0	31.0	41.0
30	36.93448724326203	39.0	35.0	41.0	31.0	41.0
31	36.84906774226415	39.0	35.0	41.0	31.0	41.0
32	36.62368372159925	39.0	35.0	41.0	30.0	41.0
33	36.4746513398021	39.0	35.0	41.0	30.0	41.0
34	36.30075275841609	39.0	35.0	41.0	30.0	41.0
35	36.35790785901362	39.0	35.0	41.0	30.0	41.0
36	36.155304369714656	39.0	35.0	41.0	30.0	41.0
37	36.18623279173884	39.0	35.0	41.0	30.0	41.0
38	35.96883484264592	39.0	35.0	41.0	30.0	41.0
39	36.06343308181453	39.0	35.0	41.0	30.0	41.0
40	35.96838834183383	39.0	35.0	40.0	29.0	41.0
41	35.86137797941901	38.0	35.0	40.0	28.0	41.0
42	36.111649176220986	39.0	35.0	41.0	30.0	41.0
43	35.82130677902106	38.0	35.0	40.0	29.0	41.0
44	35.83210670470414	38.0	35.0	40.0	29.0	41.0
45	35.8891059795149	38.0	35.0	40.0	29.0	41.0
46	35.70711644381581	38.0	35.0	40.0	28.0	41.0
47	35.44304267828568	38.0	35.0	40.0	28.0	41.0
48	35.49252335888477	38.0	35.0	40.0	28.0	41.0
49	35.65746795083097	38.0	35.0	40.0	28.0	41.0
50	35.45129545168501	38.0	35.0	40.0	27.0	41.0
51	34.33664063576921	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	7.0
12	5.0
13	4.0
14	6.0
15	7.0
16	19.0
17	39.0
18	85.0
19	266.0
20	596.0
21	1350.0
22	2030.0
23	2515.0
24	2803.0
25	2970.0
26	3287.0
27	3298.0
28	3782.0
29	4526.0
30	5743.0
31	7928.0
32	10875.0
33	14594.0
34	23725.0
35	32640.0
36	24706.0
37	33357.0
38	57363.0
39	95103.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.2181201416816	14.930507692399896	17.7377691740634	15.113602991855107
2	22.347815142670495	41.206331321582475	20.437750594834974	16.00810294091206
3	24.59380412698603	39.112272479368066	23.11495747754011	13.178965916105795
4	23.030751619689187	20.474010056756548	40.82695546379148	15.668282859762787
5	34.06531497785476	25.00554380202933	22.345118157899467	18.584023062216442
6	21.660084026058865	40.998663494213474	23.619892959671088	13.721359520056579
7	64.7674899462401	5.242339064925414	24.697188543208693	5.292982445625791
8	61.49904406873116	18.428796605395167	12.639568961900597	7.432590363973078
9	57.77870340958808	6.063421095215549	12.406129946719567	23.7517455484768
10	41.05200385968487	21.630417193577582	20.629835843526937	16.68774310321061
11	33.52951400334426	21.534824066693435	26.131385111445404	18.8042768185169
12	26.312083091104142	17.496239204569292	30.409701953216306	25.78197575111026
13	23.73316632005418	23.135634360784643	37.50367089593834	15.627528423222836
14	18.277465793243152	34.511216459997726	25.39151228926061	21.819805457498518
15	12.853529753735323	22.185996056408936	44.54579779806176	20.414676391793975
16	13.781891844917382	25.799655984609203	28.91467339514423	31.503778775329184
17	15.284412027353419	24.81915218785398	33.13875087652005	26.75768490827255
18	16.913990159002235	23.704698147471127	31.539738572276192	27.84157312125044
19	18.080885569932814	25.94109785260079	28.519115628727082	27.458900948739313
20	21.95255704122791	25.16376690859619	34.45218246000971	18.431493590166195
21	18.759327072333132	34.1024734347	28.640180278448696	18.49801921451817
22	15.890334605910592	22.615415964951186	32.01141124223118	29.48283818690704
23	17.637381407586318	31.411481963165183	29.033940055018487	21.91719657423001
24	20.911221254637315	24.330997944298275	26.150563669817146	28.607217131247264
25	14.220601367670943	37.85607690601907	25.609069060790034	22.31425266551995
26	15.477096605994497	25.656715791744826	31.004836592689372	27.8613510095713
27	23.98338657381048	28.64527458301619	24.290243507758326	23.081095335415007
28	13.767807591113135	26.733112380358758	37.131486997536754	22.36759303099135
29	20.104223478151425	24.571029588919586	28.690224329199953	26.63452260372903
30	20.13598796545462	29.69020634930148	30.92272838966036	19.25107729558354
31	21.41076276722624	23.137432350631993	26.119398512463064	29.332406369678697
32	24.918940624381943	27.602140806578245	22.393963548752495	25.08495502028732
33	20.35953803647522	20.33826182328157	29.410319263063894	29.89188087717931
34	19.905245935044622	24.544359406183887	26.656697811846357	28.89369684692514
35	22.024476635121935	20.27143653395504	25.19702972077218	32.50705711015085
36	19.028725884461174	26.337254948967054	33.67035654138673	20.96366262518504
37	22.016685345783415	19.733238239648074	31.44894008498499	26.801136329583525
38	16.56637878851444	21.65169340677123	33.46418703889052	28.317740765823807
39	18.883987701749444	21.48418068599306	28.648870562710886	30.98296104954661
40	18.358075671399376	20.13838528525109	33.175609668390734	28.327929374958792
41	16.675456839253712	22.703517467471368	28.27159235974181	32.34943333353311
42	18.372159925203622	20.07635463551749	32.214284430007254	29.337201009271634
43	22.261811294972222	20.94118775209316	30.365051872007097	26.431949080927524
44	21.22047550838163	20.488993305484467	30.92123006478757	27.369301121346336
45	17.711997986251372	19.46683607726562	29.392339364590388	33.42882657189262
46	27.03337668486632	24.029834644867037	26.531437852480927	22.405350817785717
47	15.685064098338058	20.024512594918882	41.22790719975068	23.06251610699238
48	20.710445721683158	23.0169670308595	27.07562944627906	29.19695780117828
49	17.948433651177982	16.38598047383026	38.92198522052345	26.743600654468302
50	21.818307132625726	17.95712393544018	32.70333766848663	27.521231263447465
51	19.018237610351626	18.939425722042756	26.80053699963441	35.24179966797121
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	254.0
2	388.0
3	868.5
4	1349.0
5	960.5
6	572.0
7	592.0
8	612.0
9	633.0
10	654.0
11	686.0
12	718.0
13	749.5
14	781.0
15	729.0
16	677.0
17	671.5
18	666.0
19	708.5
20	751.0
21	812.5
22	874.0
23	1041.5
24	1209.0
25	1292.0
26	1544.0
27	1713.0
28	2045.5
29	2378.0
30	3008.0
31	3638.0
32	3894.0
33	4150.0
34	5118.0
35	6086.0
36	6384.0
37	6682.0
38	7525.5
39	8369.0
40	10140.0
41	11911.0
42	15226.0
43	18541.0
44	22397.0
45	26253.0
46	52507.0
47	78761.0
48	58712.0
49	38663.0
50	37175.0
51	35687.0
52	28728.0
53	21769.0
54	18544.5
55	15320.0
56	13778.0
57	12236.0
58	10726.0
59	9216.0
60	7962.0
61	6708.0
62	5840.5
63	4973.0
64	4249.0
65	3525.0
66	2950.5
67	2376.0
68	1995.5
69	1615.0
70	1282.0
71	949.0
72	801.0
73	653.0
74	493.5
75	320.0
76	306.0
77	199.0
78	92.0
79	59.5
80	27.0
81	21.0
82	15.0
83	11.0
84	7.0
85	4.5
86	2.0
87	3.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	333706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.09573503262906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16698675638743	22.62205716621374
2	10.526998145256435	6.336354937372306
3	3.69401681571918	3.3352245387588173
4	1.9009998158781258	2.2884794702297886
5	1.1469997994375016	1.7259901023174866
6	0.722999872631357	1.3055527557202729
7	0.5189999085580066	1.0933778610944336
8	0.4579999193053556	1.102707537310358
9	0.42699992476721754	1.156578893527205
>10	5.119999097911367	34.17309919391805
>50	0.25899995436700074	4.716301806001777
>100	0.04199999260005417	2.451899101110106
>500	0.005999998942864883	1.2390411929876406
>1k	0.006999998766675696	4.869489070327914
>5k	0.0	0.0
>10k+	0.0029999994714324414	11.5838463731101
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	13862	4.153955877329146	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	12435	3.7263339586342465	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	12193	3.653815034791104	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3703	1.109659400789917	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	2769	0.8297723145523305	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	2284	0.6844348018914853	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT	2092	0.6268991267762641	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA	1960	0.5873433501345495	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC	1766	0.5292083450702115	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1606	0.4812619491408605	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	987	0.29576932988918386	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.2250483958933912	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT	700	0.20976548219091054	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATG	643	0.19268457864107927	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTAT	530	0.15882243651597514	No Hit
ATGAAGGGGTTCCAGAGGTCATCAAAACCTTTATTTGTCTCTCCTTTGAGC	506	0.1516304771265725	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGT	465	0.1393442131696763	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	429	0.12855627408557233	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTCTCCT	406	0.12166397967072813	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.98994923675331E-4	0.0	0.0	0.3170455430828334	0.0
2	8.98994923675331E-4	0.0	0.0	2.253780273654055	0.0
3	8.98994923675331E-4	0.0	0.0	2.7563184359885646	0.0
4	8.98994923675331E-4	0.0	0.0	4.042180841818847	0.0
5	8.98994923675331E-4	0.0	0.0	9.212300647875676	0.0
6	8.98994923675331E-4	0.0	0.0	10.394778637483293	0.0
7	8.98994923675331E-4	0.0	0.0	12.222135652340683	0.0
8	8.98994923675331E-4	0.0	0.0	14.258658819439866	0.0
9	8.98994923675331E-4	0.0	0.0	14.884359286317897	0.0
10	8.98994923675331E-4	0.0	0.0	20.0856442497288	0.0
11	8.98994923675331E-4	0.0	0.0	21.593558401706893	0.0
12	8.98994923675331E-4	0.0	0.0	27.471786542645322	0.0
13	8.98994923675331E-4	0.0	0.0	28.30185852217221	0.0
14	8.98994923675331E-4	0.0	0.0	28.718392836808448	0.0
15	8.98994923675331E-4	0.0	0.0	29.75403498888243	0.0
16	0.0011986598982337746	0.0	0.0	30.679700095293462	0.0
17	0.0011986598982337746	0.0	0.0	31.693167039250117	0.0
18	0.0011986598982337746	0.0	0.0	32.981426764876865	0.0
19	0.0014983248727922184	0.0	0.0	34.37756588134466	0.0
20	0.0014983248727922184	0.0	0.0	35.13961391164678	0.0
21	0.0014983248727922184	0.0	0.0	35.849220571401176	0.0
22	0.0014983248727922184	0.0	0.0	36.607972286983156	0.0
23	0.0014983248727922184	0.0	0.0	37.22108682492973	0.0
24	0.0014983248727922184	0.0	0.0	37.698752794375885	0.0
25	0.001797989847350662	0.0	0.0	38.114687779063004	0.0
26	0.001797989847350662	0.0	0.0	38.503952581014424	0.0
27	0.001797989847350662	0.0	0.0	39.03855489562669	0.0
28	0.001797989847350662	0.0	0.0	39.43141567727281	0.0
29	0.001797989847350662	0.0	0.0	39.86263357566241	0.0
30	0.001797989847350662	0.0	0.0	40.44488262122947	0.0
31	0.001797989847350662	0.0	0.0	40.87669984956818	0.0
32	0.001797989847350662	0.0	0.0	41.25787369720652	0.0
33	0.001797989847350662	0.0	0.0	41.63784888494663	0.0
34	0.001797989847350662	0.0	0.0	42.04599258029523	0.0
35	0.001797989847350662	0.0	0.0	42.45323728072015	0.0
36	0.001797989847350662	0.0	0.0	42.83231347353659	0.0
37	0.001797989847350662	0.0	0.0	43.2021000521417	0.0
38	0.001797989847350662	0.0	0.0	43.55240840740053	0.0
39	0.001797989847350662	0.0	0.0	43.91919833626006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	85	0.0	45.000004	2
CGGGCAT	45	3.8380676E-10	45.000004	6
TCACGAC	25	3.884836E-5	45.0	12
ATTTACG	20	7.026085E-4	45.0	10
TTAGGCG	25	3.884836E-5	45.0	1
AGCCGGT	20	7.026085E-4	45.0	15
GATACCA	20	7.026085E-4	45.0	11
GTACCGG	35	1.2084638E-7	45.0	2
GGTACCG	25	3.884836E-5	45.0	1
TTTACGT	25	3.884836E-5	45.0	18
GCGAACC	50	2.1827873E-11	45.0	33
GTTTACG	25	3.884836E-5	45.0	17
CGCATGG	25	3.884836E-5	45.0	2
CTGAGCG	20	7.026085E-4	45.0	1
CCGGATT	20	7.026085E-4	45.0	42
TGAACAC	20	7.026085E-4	45.0	17
AATGGTC	40	6.7902874E-9	45.0	10
TTCCGTC	20	7.026085E-4	45.0	42
GAATATG	20	7.026085E-4	45.0	1
TCGATCA	20	7.026085E-4	45.0	17
>>END_MODULE