##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935752.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592235
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02223441708106	33.0	31.0	34.0	30.0	34.0
2	32.05882462198283	33.0	31.0	34.0	30.0	34.0
3	32.116082298411946	33.0	31.0	34.0	30.0	34.0
4	35.78234484621814	37.0	35.0	37.0	35.0	37.0
5	34.825278816685945	37.0	35.0	37.0	33.0	37.0
6	35.297160755443365	37.0	35.0	37.0	33.0	37.0
7	35.842020481734444	37.0	35.0	37.0	35.0	37.0
8	35.80541508016243	37.0	35.0	37.0	35.0	37.0
9	37.63145541887933	39.0	37.0	39.0	35.0	39.0
10	37.364632282793146	39.0	37.0	39.0	34.0	39.0
11	37.289336158788316	39.0	37.0	39.0	34.0	39.0
12	37.132457554855755	39.0	37.0	39.0	34.0	39.0
13	36.989353888236934	39.0	37.0	39.0	33.0	39.0
14	38.03737705471646	40.0	37.0	41.0	33.0	41.0
15	38.12277052183677	40.0	37.0	41.0	33.0	41.0
16	38.22896147644094	40.0	37.0	41.0	34.0	41.0
17	38.13519802105583	40.0	37.0	41.0	33.0	41.0
18	38.142485668695706	40.0	37.0	41.0	34.0	41.0
19	38.05197598926102	40.0	37.0	41.0	34.0	41.0
20	37.97673558638041	40.0	36.0	41.0	33.0	41.0
21	37.93397891039874	40.0	36.0	41.0	33.0	41.0
22	37.987037240284685	40.0	36.0	41.0	34.0	41.0
23	37.978018860756286	40.0	36.0	41.0	34.0	41.0
24	37.948912171688605	40.0	36.0	41.0	34.0	41.0
25	37.82747389127627	40.0	36.0	41.0	33.0	41.0
26	37.80354757824175	40.0	36.0	41.0	33.0	41.0
27	37.78997019764114	40.0	36.0	41.0	33.0	41.0
28	37.65760044576899	40.0	36.0	41.0	33.0	41.0
29	37.58362812059402	40.0	36.0	41.0	33.0	41.0
30	37.44560858443017	40.0	35.0	41.0	33.0	41.0
31	37.445110471350056	40.0	35.0	41.0	33.0	41.0
32	37.29370773426089	40.0	35.0	41.0	33.0	41.0
33	37.13746401344061	39.0	35.0	41.0	32.0	41.0
34	37.04002971793292	39.0	35.0	41.0	32.0	41.0
35	36.93017636580074	39.0	35.0	41.0	31.0	41.0
36	36.863319459336246	39.0	35.0	41.0	31.0	41.0
37	36.82205205703817	39.0	35.0	41.0	31.0	41.0
38	36.6901331397165	39.0	35.0	41.0	31.0	41.0
39	36.597874154685215	39.0	35.0	41.0	31.0	41.0
40	36.36859185965031	39.0	35.0	41.0	30.0	41.0
41	36.29241432877152	39.0	35.0	41.0	30.0	41.0
42	36.31467745067414	39.0	35.0	41.0	30.0	41.0
43	36.20980016378633	38.0	35.0	41.0	30.0	41.0
44	36.1191013702331	38.0	35.0	40.0	30.0	41.0
45	36.05255346273017	38.0	35.0	40.0	30.0	41.0
46	35.89423624068149	38.0	35.0	40.0	30.0	41.0
47	35.68416591386865	38.0	35.0	40.0	29.0	41.0
48	35.65222420154162	38.0	35.0	40.0	29.0	41.0
49	35.6295507695425	37.0	35.0	40.0	29.0	41.0
50	35.49584371068917	37.0	35.0	40.0	29.0	41.0
51	34.261207122172785	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	13.0
11	8.0
12	10.0
13	12.0
14	17.0
15	26.0
16	44.0
17	82.0
18	161.0
19	340.0
20	602.0
21	1132.0
22	1744.0
23	2214.0
24	2950.0
25	3734.0
26	4635.0
27	5565.0
28	6347.0
29	7676.0
30	9410.0
31	11989.0
32	16209.0
33	22213.0
34	40232.0
35	51137.0
36	45904.0
37	68785.0
38	113714.0
39	175173.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.58134017746334	16.644068655179108	19.770530279365456	17.004060887992097
2	28.258883720144873	28.900520908085475	24.3965655525256	18.44402981924405
3	30.73948685910154	28.8773881989413	24.420204817344466	15.962920124612696
4	27.41006526125609	24.648830278521196	29.222521465296715	18.718582994926
5	26.486107710621624	29.83173909005716	23.879541060558733	19.802612138762484
6	24.615228752100098	35.272653591901864	24.51678809931868	15.595329556679358
7	75.94468412032386	5.84953607942793	13.183955693263655	5.021824106984559
8	75.78883382441091	8.399537345817116	9.62523322667522	6.186395603096743
9	69.30458348459648	7.348772024618606	12.373804317542868	10.97284017324204
10	40.39578883382441	25.47282750935017	18.34103016539043	15.790353491434988
11	32.92037789053332	23.549773316335575	24.693069474110786	18.836779319020323
12	27.104274485634928	21.439124671794136	29.530507315508203	21.926093527062736
13	25.020135588068925	24.52320447119809	32.22285072648526	18.233809214247724
14	21.151569900461812	28.607394024331555	28.702119935498576	21.538916139708057
15	18.47273464080981	23.275557844436754	36.204716033331366	22.046991481422072
16	22.53632426317256	24.674326914147258	28.823862149315726	23.96548667336446
17	22.25940716100872	24.706746477327414	28.712082197100813	24.321764164563053
18	25.59186809290231	21.92676893462899	29.000987783565645	23.480375188903054
19	23.749525104054978	26.09386476652005	25.643874475503814	24.512735653921165
20	25.77608550659789	25.028071626972405	28.39227671447989	20.803566151949816
21	23.824326492017526	29.216105093417312	27.346576950028282	19.61299146453688
22	23.042035678404687	22.699266338531157	28.202993744037418	26.05570423902674
23	20.737882766131687	28.226295305073158	27.370722770521837	23.66509915827332
24	22.61838628247233	23.646525450201356	28.77236232238892	24.962725944937397
25	21.242749921906	28.416929090648136	26.783287039773064	23.5570339476728
26	21.1856779825576	24.25540537117867	28.60199076380153	25.9569258824622
27	22.699266338531157	24.6704433206413	27.122003934249072	25.508286406578467
28	19.46068705834677	25.451383319121636	29.481202563171717	25.606727059359883
29	22.063538966795278	24.149366383276906	28.369988264793538	25.417106385134282
30	22.52636200157032	24.555455182486682	29.006728747878796	23.9114540680642
31	25.96975862622101	23.779918444536374	24.657779428774052	25.59254350046856
32	25.69841363647876	26.02649286178628	25.30110513563028	22.973988366104674
33	23.1722204868	26.16157437503694	23.31456263138788	27.351642506775182
34	23.586414176804816	24.782223272856214	27.957820797487482	23.673541752851488
35	22.27663005394818	26.6873791653651	26.316411559600496	24.719579221086224
36	22.58073231065371	28.895961907013263	25.12988931758508	23.393416464747947
37	23.39274105718169	26.18487593607267	25.5739697923966	24.848413214349033
38	21.613210971995915	30.084848075510568	23.655474600454212	24.646466352039308
39	25.943755434920256	25.548304304878975	24.600538637534086	23.907401622666676
40	25.165685918596502	26.78514441058026	25.98394218511233	22.06522748571091
41	21.774126824655752	26.515319087862082	25.38080322844817	26.329750859033997
42	23.237228465051878	25.096963198730233	27.436912712014657	24.228895624203233
43	23.066688054572932	25.09223534576646	26.48914704466977	25.35192955499084
44	23.40692461607301	23.337357636748926	27.51914358320599	25.73657416397207
45	22.327792177091865	22.969598216924027	27.02710917119049	27.67550043479362
46	25.938352174390232	24.57318463110083	25.533107634638274	23.955355559870657
47	20.878874095587054	25.551512490818677	30.59866438153773	22.97094903205653
48	22.96790969800839	25.989345445642353	26.16089896747068	24.88184588887857
49	23.78262007480139	22.79145947132473	29.75102788588989	23.674892567983992
50	22.32357087980278	23.227266203449645	29.24058861769399	25.208574299053588
51	21.395223179987674	22.837049482046822	25.836365631885993	29.931361706079514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	670.5
2	1165.0
3	1755.5
4	2346.0
5	1626.5
6	907.0
7	934.5
8	962.0
9	1013.0
10	1064.0
11	1045.0
12	1026.0
13	1111.0
14	1196.0
15	1195.5
16	1195.0
17	1224.5
18	1254.0
19	1325.5
20	1397.0
21	1426.0
22	1455.0
23	1848.0
24	2241.0
25	2374.0
26	3157.5
27	3808.0
28	4690.0
29	5572.0
30	6254.0
31	6936.0
32	7981.0
33	9026.0
34	10010.0
35	10994.0
36	12068.5
37	13143.0
38	14940.0
39	16737.0
40	18598.0
41	20459.0
42	23544.0
43	26629.0
44	31553.0
45	36477.0
46	52718.0
47	68959.0
48	63958.5
49	58958.0
50	59410.5
51	59863.0
52	53327.0
53	46791.0
54	42792.5
55	38794.0
56	35415.0
57	32036.0
58	29333.0
59	26630.0
60	25406.0
61	24182.0
62	21570.5
63	18959.0
64	16968.0
65	14977.0
66	12956.0
67	10935.0
68	9355.0
69	7775.0
70	6704.5
71	5634.0
72	4662.0
73	3690.0
74	3134.5
75	2099.5
76	1620.0
77	1135.0
78	650.0
79	495.0
80	340.0
81	218.5
82	97.0
83	73.0
84	49.0
85	42.0
86	35.0
87	19.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.197041629151297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01553064862748	23.090655908489545
2	10.314647593770047	6.435729807457388
3	3.634739910551858	3.4017939690187196
4	1.8454011752051684	2.3028422914144144
5	1.207631798570435	1.8837269746344358
6	0.8298510758659552	1.5533339135871715
7	0.6356145587007916	1.388050569351826
8	0.533444795291947	1.331351958846157
9	0.4824503733318554	1.3545921942750128
>10	6.169513204286407	43.616711416493644
>50	0.26853488295795136	5.181301565750708
>100	0.05501424579868643	3.066706548066678
>500	0.002178782011829166	0.5439423538738536
>1k	0.00381286852070104	1.803455233259359
>5k	0.0016340865088718742	3.0458052954810846
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	6578	1.1107077427034877	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	5858	0.9891343807779007	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	5488	0.9266591808994741	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2819	0.4759934823169856	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1933	0.32639070639188833	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1312	0.22153368173106958	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1284	0.21680582876729676	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.19789441691220547	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1077	0.18185348721369052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	1016	0.17155352182832828	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	953	0.16091585265983943	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	951	0.1605781488767128	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	742	0.1252881035399799	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6885189156331523E-4	0.0	0.0	0.19434852718937584	0.0
2	1.6885189156331523E-4	0.0	0.0	1.197159911183905	0.0
3	1.6885189156331523E-4	0.0	0.0	1.5529308467078102	0.0
4	1.6885189156331523E-4	0.0	0.0	2.189164774118382	0.0
5	1.6885189156331523E-4	0.0	0.0	4.2937347505635435	0.0
6	1.6885189156331523E-4	0.0	0.0	5.423522757013686	0.0
7	1.6885189156331523E-4	0.0	0.0	6.519202681368038	0.0
8	1.6885189156331523E-4	0.0	0.0	8.253818163397975	0.0
9	1.6885189156331523E-4	0.0	0.0	8.98342718684306	0.0
10	1.6885189156331523E-4	0.0	0.0	11.183736185804621	0.0
11	1.6885189156331523E-4	0.0	0.0	12.445566371457277	0.0
12	1.6885189156331523E-4	0.0	0.0	14.806622371187114	0.0
13	1.6885189156331523E-4	0.0	0.0	15.387979433839607	0.0
14	1.6885189156331523E-4	0.0	0.0	15.665065387895007	0.0
15	1.6885189156331523E-4	0.0	0.0	16.16537354259711	0.0
16	1.6885189156331523E-4	0.0	0.0	16.842300775874442	0.0
17	1.6885189156331523E-4	0.0	0.0	17.598419546294966	0.0
18	1.6885189156331523E-4	0.0	0.0	18.55868025361554	0.0
19	1.6885189156331523E-4	0.0	0.0	19.2442189333626	0.0
20	1.6885189156331523E-4	0.0	0.0	19.844487407870187	0.0
21	1.6885189156331523E-4	0.0	0.0	20.406088799209773	0.0
22	1.6885189156331523E-4	0.0	0.0	21.013280201271456	0.0
23	1.6885189156331523E-4	0.0	0.0	21.561204589394414	0.0
24	1.6885189156331523E-4	0.0	0.0	22.001739174483102	0.0
25	1.6885189156331523E-4	0.0	0.0	22.44159835200554	0.0
26	1.6885189156331523E-4	0.0	0.0	22.830633110167415	0.0
27	1.6885189156331523E-4	0.0	0.0	23.311692149231302	0.0
28	1.6885189156331523E-4	0.0	0.0	23.72774321004331	0.0
29	1.6885189156331523E-4	0.0	0.0	24.19934654317965	0.0
30	1.6885189156331523E-4	0.0	0.0	24.842165694361192	0.0
31	1.6885189156331523E-4	0.0	0.0	25.275270796221093	0.0
32	1.6885189156331523E-4	0.0	0.0	25.7419774245021	0.0
33	1.6885189156331523E-4	0.0	0.0	26.179810379325776	0.0
34	1.6885189156331523E-4	0.0	0.0	26.62473511359511	0.0
35	1.6885189156331523E-4	0.0	0.0	27.13416127044163	0.0
36	1.6885189156331523E-4	0.0	0.0	27.553420517193345	0.0
37	1.6885189156331523E-4	0.0	0.0	27.966432243957215	0.0
38	1.6885189156331523E-4	0.0	0.0	28.432125760888837	0.0
39	1.6885189156331523E-4	0.0	0.0	29.020405751095428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	25	3.8885075E-5	45.0	1
TCGTTTA	20	7.0305134E-4	45.0	38
CGAACGT	25	3.8885075E-5	45.0	37
CGACGGT	35	1.210592E-7	45.0	28
TTGTACG	35	1.210592E-7	45.0	1
TAACGCC	35	1.210592E-7	45.0	12
CCGCTAG	25	3.8885075E-5	45.0	1
TATTGCG	20	7.0305134E-4	45.0	1
TGTTACG	25	3.8885075E-5	45.0	1
GTATCCG	20	7.0305134E-4	45.0	1
ATGGTCG	20	7.0305134E-4	45.0	44
CATCGTT	20	7.0305134E-4	45.0	36
CGGTCTA	35	1.210592E-7	45.0	31
CCGAACG	25	3.8885075E-5	45.0	36
TCGACAC	20	7.0305134E-4	45.0	42
ACGACAC	20	7.0305134E-4	45.0	34
TCCAACG	30	2.1637134E-6	44.999996	38
TACACGG	30	2.1637134E-6	44.999996	2
CGAGTAC	65	0.0	41.53846	27
CCGAGTA	65	0.0	41.53846	26
>>END_MODULE