##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935751.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292176
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00515719220911	33.0	31.0	34.0	30.0	34.0
2	32.05546071123438	33.0	31.0	34.0	30.0	34.0
3	32.061150338653555	33.0	31.0	34.0	30.0	34.0
4	35.743882412302966	37.0	35.0	37.0	33.0	37.0
5	34.80984014561484	37.0	35.0	37.0	33.0	37.0
6	35.30834034992137	37.0	35.0	37.0	33.0	37.0
7	35.91239041740444	37.0	35.0	37.0	35.0	37.0
8	35.914750776983944	37.0	35.0	37.0	35.0	37.0
9	37.746143714168966	39.0	38.0	39.0	35.0	39.0
10	37.401347030125926	39.0	37.0	39.0	34.0	39.0
11	37.3812065848615	39.0	37.0	39.0	34.0	39.0
12	37.41218920642389	39.0	37.0	39.0	35.0	39.0
13	37.41366578546576	39.0	37.0	39.0	35.0	39.0
14	38.59304769628905	40.0	38.0	41.0	34.0	41.0
15	38.66813963422939	40.0	38.0	41.0	35.0	41.0
16	38.6360673778185	40.0	38.0	41.0	35.0	41.0
17	38.599995666225034	40.0	38.0	41.0	34.0	41.0
18	38.512263809264375	40.0	38.0	41.0	34.0	41.0
19	38.390714577580766	40.0	37.0	41.0	34.0	41.0
20	38.35788468443927	40.0	37.0	41.0	34.0	41.0
21	38.25679474003541	40.0	37.0	41.0	34.0	41.0
22	38.30143726551182	40.0	37.0	41.0	34.0	41.0
23	38.28557410136081	40.0	37.0	41.0	34.0	41.0
24	38.251992762595805	40.0	37.0	41.0	34.0	41.0
25	38.07675192852986	40.0	37.0	41.0	34.0	41.0
26	38.11861387303278	40.0	37.0	41.0	34.0	41.0
27	38.1348260608462	40.0	37.0	41.0	34.0	41.0
28	38.0085615272223	40.0	37.0	41.0	34.0	41.0
29	37.97090411832444	40.0	37.0	41.0	34.0	41.0
30	37.82274086502148	40.0	36.0	41.0	33.0	41.0
31	37.743550414185066	40.0	37.0	41.0	33.0	41.0
32	37.61525519743441	40.0	37.0	41.0	33.0	41.0
33	37.38650539864538	40.0	36.0	41.0	33.0	41.0
34	37.2300096890826	40.0	36.0	41.0	32.0	41.0
35	37.13084208343136	40.0	36.0	41.0	31.0	41.0
36	37.04835099862557	40.0	36.0	41.0	31.0	41.0
37	37.02164333651144	40.0	36.0	41.0	31.0	41.0
38	36.91591780067112	40.0	36.0	41.0	31.0	41.0
39	36.84165469719295	40.0	36.0	41.0	31.0	41.0
40	36.74366804521985	40.0	35.0	41.0	30.0	41.0
41	36.64550804224812	40.0	35.0	41.0	30.0	41.0
42	36.65006160151558	40.0	35.0	41.0	30.0	41.0
43	36.57055540421738	39.0	35.0	41.0	30.0	41.0
44	36.437682637659265	39.0	35.0	41.0	30.0	41.0
45	36.40049420512376	39.0	35.0	41.0	30.0	41.0
46	36.280608833471604	39.0	35.0	41.0	30.0	41.0
47	36.1947443691881	39.0	35.0	41.0	30.0	41.0
48	36.123464605440745	39.0	35.0	41.0	29.0	41.0
49	36.095500922474955	39.0	35.0	41.0	29.0	41.0
50	35.98754426641572	39.0	35.0	41.0	29.0	41.0
51	34.862962940032936	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	17.0
10	22.0
11	33.0
12	23.0
13	20.0
14	25.0
15	42.0
16	82.0
17	147.0
18	284.0
19	516.0
20	915.0
21	1531.0
22	2429.0
23	3831.0
24	6131.0
25	9891.0
26	14063.0
27	15777.0
28	16142.0
29	16741.0
30	18740.0
31	22742.0
32	28871.0
33	38483.0
34	62712.0
35	83119.0
36	91707.0
37	138475.0
38	255029.0
39	463330.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.541576379688216	18.05427434033754	21.574073500823417	21.830075779150828
2	31.743353846534838	25.114225925880067	26.236209308948627	16.90621091863647
3	30.271185968474885	23.41654697192952	30.324816433674666	15.987450625920927
4	27.22183355827689	24.93762459603026	30.305701390522653	17.534840455170194
5	23.94186240883595	30.41273015440621	27.443475192233873	18.201932244523967
6	24.73277634006513	33.01748368643281	27.01303847153948	15.236701501962582
7	77.20867745570263	5.332400539864539	12.46664541053231	4.992276593900521
8	78.66320067854534	4.708569111328488	11.387922388281472	5.240307821844702
9	70.945134408935	7.87849333217766	14.397264768885973	6.779107490001363
10	31.640504079939575	35.00451950817845	19.386677975755624	13.968298436126346
11	24.54828134867077	25.039081363529426	32.46817770953802	17.944459578261785
12	23.322674310620226	22.255172669976844	33.87293990911455	20.54921311028838
13	22.246892064238928	22.136845135647157	36.434046136130064	19.18221666398385
14	19.26494533252436	26.616033729151447	33.168469310682134	20.950551627642056
15	18.62873168980077	26.30028726736915	35.91252275231857	19.15845829051151
16	21.42672515199168	26.57161253575364	32.85303240425453	19.148629908000146
17	21.20345835242258	26.256485184680724	30.91568021693639	21.624376245960303
18	21.594271987716844	25.842919230816854	31.49803122794418	21.06477755352212
19	21.24122410569458	28.033255531754186	29.70431272520152	21.02120763734971
20	22.052026968462503	26.812678768217335	30.092882084174295	21.042412179145874
21	22.450269932269286	27.90293272743032	30.509930535778405	19.136866804521986
22	21.065706219586186	24.93460643132205	30.939438590408734	23.060248758683027
23	19.70018016121643	27.06628199254591	30.94052203415015	22.293015812087518
24	20.979649830982776	25.410083456123623	31.651028961998982	21.959237750894616
25	21.034828072956007	27.00723430863907	29.534057280122834	22.423880338282093
26	20.323624645559118	27.42482448211389	29.104007503621798	23.147543368705193
27	21.486778890801254	25.840210621463328	29.27797761295675	23.395032874778668
28	20.169853023117593	26.214308267604412	30.813140005695814	22.802698703582173
29	20.93190091752207	24.42631653892349	29.07390324537834	25.5678792981761
30	21.358622973960202	23.95741756540905	30.19960129270316	24.48435816792759
31	21.226288059830857	24.156306880796425	29.656486422902145	24.960918636470574
32	22.302457250405517	24.459593739552506	29.47044365473434	23.767505355307637
33	21.742162058419286	24.10430158120875	29.880604499696634	24.272931860675325
34	20.36247384257253	23.622710838152077	32.741747254244004	23.27306806503139
35	20.550838275900496	23.31849531333193	31.132988075927738	24.997678334839836
36	20.167995690989464	24.3882412302968	31.894958581493544	23.548804497220193
37	20.703990787632645	24.28036118918785	32.7850850039004	22.230563019279106
38	20.03318433402261	24.725269622713935	31.98890863164151	23.252637411621947
39	21.518043981624793	23.48124404105942	31.962596426492983	23.0381155508228
40	21.974173796758336	23.14413825980362	32.91068708906526	21.97100085437278
41	19.50446378821461	24.489388442441275	32.10506927848838	23.901078490855735
42	20.63488255469843	24.807688735899752	30.618507076435407	23.938921632966405
43	21.240140661953173	23.699635343792178	31.449740592612773	23.610483401641883
44	22.021613154864355	24.452396577555998	29.85274451777467	23.67324574980498
45	21.180319089659612	23.119141664912522	29.77001584923416	25.930523396193706
46	22.165788561310535	23.6929025148277	29.37022510865393	24.771083815207838
47	20.96734500563391	23.11596872252696	31.279098203340723	24.63758806849841
48	20.6755116950013	22.763617340052747	31.255649385223066	25.305221579722886
49	20.793994007008333	22.355700771411943	31.936052054828444	24.91425316675128
50	19.93513267542502	22.167336338083977	33.09897413355456	24.798556852936443
51	19.570089523408576	21.6729764366464	31.540982033407218	27.215952006537808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	833.0
2	1377.0
3	8999.5
4	16622.0
5	11198.0
6	5774.0
7	5520.0
8	5266.0
9	5411.0
10	5556.0
11	5528.5
12	5501.0
13	5435.5
14	5370.0
15	5047.5
16	4725.0
17	4792.0
18	4859.0
19	4669.5
20	4480.0
21	4955.0
22	5430.0
23	5666.5
24	5903.0
25	6664.0
26	9204.5
27	10984.0
28	13068.0
29	15152.0
30	17747.5
31	20343.0
32	23220.5
33	26098.0
34	29937.0
35	33776.0
36	37231.0
37	40686.0
38	44548.0
39	48410.0
40	53567.5
41	58725.0
42	64991.0
43	71257.0
44	80425.5
45	89594.0
46	110907.0
47	132220.0
48	138677.0
49	145134.0
50	140107.5
51	135081.0
52	114566.0
53	94051.0
54	80968.0
55	67885.0
56	61149.0
57	54413.0
58	50122.0
59	45831.0
60	41682.0
61	37533.0
62	33682.5
63	29832.0
64	24907.0
65	19982.0
66	16535.5
67	13089.0
68	10713.0
69	8337.0
70	7040.5
71	5744.0
72	4826.0
73	3908.0
74	3224.0
75	1994.0
76	1448.0
77	1160.0
78	872.0
79	620.5
80	369.0
81	260.5
82	152.0
83	117.0
84	82.0
85	62.0
86	42.0
87	30.0
88	18.0
89	11.5
90	5.0
91	3.0
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1292176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.72812744600574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99519726808832	25.002583862264956
2	9.796287180935693	6.80413419434414
3	4.021257767794499	4.189522567596239
4	2.3270405234469997	3.2325503948114926
5	1.617857543902941	2.809258148707158
6	1.2065790778684358	2.5141339193939474
7	1.0250143585545795	2.4917780494508484
8	0.8364766558221801	2.323941432720108
9	0.7437525850495265	2.324622110570656
>10	6.294497693201325	40.23761846316336
>50	0.09312483778635455	2.1595927545556486
>100	0.038146229440064794	2.290471891076042
>500	0.0015894260363468766	0.397187525728074
>1k	0.002951791210358485	2.4390326301955643
>5k	2.2706086233526806E-4	0.7835720554218526
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9937	0.7690128898849693	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	4008	0.31017446539790244	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	3750	0.2902081450204926	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3362	0.2601812756157056	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	3248	0.2513589480070826	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2782	0.2152957491858694	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	2642	0.20446131177177104	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	2325	0.1799290499127054	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	2121	0.1641417268235906	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1922	0.14874134792783644	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1411	0.10919565136637735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.512772253934449E-4	0.0	0.0	0.17706566288183653	0.0
2	8.512772253934449E-4	0.0	0.0	0.7896757098104283	0.0
3	9.286660640655762E-4	0.0	0.0	1.3324810242567575	0.0
4	9.286660640655762E-4	0.0	0.0	2.0172174688277758	0.0
5	9.286660640655762E-4	0.0	0.0	3.644240413070665	0.0
6	9.286660640655762E-4	0.0	0.0	5.62438862817449	0.0
7	9.286660640655762E-4	0.0	0.0	6.994944961057937	0.0
8	9.286660640655762E-4	0.0	0.0	8.989023167122745	0.0
9	9.286660640655762E-4	0.0	0.0	10.069835688017731	0.0
10	9.286660640655762E-4	0.0	0.0	11.589288146506359	0.0
11	0.0010060549027377077	0.0	0.0	12.85583388021446	0.0
12	0.0010060549027377077	0.0	0.0	14.193577345500923	0.0
13	0.0010060549027377077	0.0	0.0	14.923121927663106	0.0
14	0.0010060549027377077	0.0	0.0	15.357273312613762	0.0
15	0.0010834437414098388	0.0	0.0	15.753272000099058	0.0
16	0.0010834437414098388	0.0	0.0	16.32308601924196	0.0
17	0.0010834437414098388	0.0	0.0	16.976789539505454	0.0
18	0.0010834437414098388	0.0	0.0	17.762286252027586	0.0
19	0.0010834437414098388	0.0	0.0	18.27730897339062	0.0
20	0.0010834437414098388	0.0	0.0	18.812684959324425	0.0
21	0.0011608325800819702	0.0	0.0	19.363693490670002	0.0
22	0.0011608325800819702	0.0	0.0	19.96051621450948	0.0
23	0.0011608325800819702	0.0	0.0	20.5447245576454	0.0
24	0.0011608325800819702	0.0	0.0	21.031732519409122	0.0
25	0.0012382214187541016	0.0	0.0	21.48089733906217	0.0
26	0.0012382214187541016	0.0	0.0	21.920698109235893	0.0
27	0.001315610257426233	0.0	0.0	22.366689986503385	0.0
28	0.0013929990960983643	0.0	0.0	22.836981959113928	0.0
29	0.0013929990960983643	0.0	0.0	23.33954507745075	0.0
30	0.0013929990960983643	0.0	0.0	23.95316117928208	0.0
31	0.001547776773442627	0.0	0.0	24.47685145057639	0.0
32	0.0016251656121147584	0.0	0.0	25.00069649954805	0.0
33	0.0016251656121147584	0.0	0.0	25.508754225430593	0.0
34	0.0016251656121147584	0.0	0.0	25.982915639974742	0.0
35	0.0016251656121147584	0.0	0.0	26.477275541412315	0.0
36	0.0016251656121147584	0.0	0.0	26.96877205581902	0.0
37	0.0016251656121147584	0.0	0.0	27.440611805203005	0.0
38	0.0016251656121147584	0.0	0.0	27.951997251148452	0.0
39	0.0016251656121147584	0.0	0.0	28.468180805091567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	25	3.8910766E-5	45.0	1
TATAGCG	40	6.8157533E-9	45.0	1
CGTTTTT	5840	0.0	42.803936	1
TGCAACG	45	1.9286745E-8	40.0	1
TAACGCG	40	3.4586628E-7	39.375	1
TAAGACG	40	3.4586628E-7	39.375	1
ACGGGTA	110	0.0	38.86364	5
CGCGTAA	30	1.1400004E-4	37.499996	13
CGTTTCT	525	0.0	36.85714	1
TGCGACG	55	2.750312E-9	36.818184	1
CGTTAGG	190	0.0	36.71053	2
TTTACGG	155	0.0	36.290325	2
CGCGGAA	25	0.0021072056	36.0	4
GCGAAGT	25	0.0021072056	36.0	43
AATTCGC	25	0.0021072056	36.0	16
TACAGCG	50	4.879803E-8	36.0	1
CGTTCTG	345	0.0	35.869564	1
TAGTAGG	575	0.0	35.217392	2
TTACGGG	475	0.0	35.05263	3
ATAGGGA	1535	0.0	35.032578	4
>>END_MODULE