##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935750.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	552467
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16109016466142	33.0	31.0	34.0	30.0	34.0
2	32.17872922726606	34.0	31.0	34.0	30.0	34.0
3	32.244378397261734	34.0	31.0	34.0	30.0	34.0
4	35.87397799325571	37.0	35.0	37.0	35.0	37.0
5	34.916130737220506	37.0	35.0	37.0	33.0	37.0
6	35.39659020357777	37.0	35.0	37.0	33.0	37.0
7	35.934276617426924	37.0	35.0	37.0	35.0	37.0
8	35.91893452459604	37.0	35.0	37.0	35.0	37.0
9	37.76908123019113	39.0	38.0	39.0	35.0	39.0
10	37.470124007406774	39.0	37.0	39.0	35.0	39.0
11	37.36871523548013	39.0	37.0	39.0	35.0	39.0
12	37.19979835899701	39.0	37.0	39.0	34.0	39.0
13	37.074145605076865	39.0	37.0	39.0	33.0	39.0
14	38.10132912916065	40.0	37.0	41.0	33.0	41.0
15	38.22053624922393	40.0	37.0	41.0	33.0	41.0
16	38.29062550342373	40.0	37.0	41.0	34.0	41.0
17	38.30795866540445	40.0	37.0	41.0	34.0	41.0
18	38.29780240267745	40.0	37.0	41.0	34.0	41.0
19	38.231764069166125	40.0	37.0	41.0	34.0	41.0
20	38.176343564411994	40.0	37.0	41.0	34.0	41.0
21	38.071837774925925	40.0	36.0	41.0	34.0	41.0
22	38.12430425708685	40.0	36.0	41.0	34.0	41.0
23	38.15474589432491	40.0	36.0	41.0	34.0	41.0
24	38.11076317680513	40.0	36.0	41.0	34.0	41.0
25	37.94793535179477	40.0	36.0	41.0	34.0	41.0
26	37.95516474287152	40.0	36.0	41.0	34.0	41.0
27	37.94274771162802	40.0	36.0	41.0	34.0	41.0
28	37.81059140183939	40.0	36.0	41.0	33.0	41.0
29	37.753599762519755	40.0	36.0	41.0	33.0	41.0
30	37.633426068887374	40.0	36.0	41.0	33.0	41.0
31	37.554389673953374	40.0	35.0	41.0	33.0	41.0
32	37.456778413914314	40.0	35.0	41.0	33.0	41.0
33	37.32891014304927	40.0	35.0	41.0	33.0	41.0
34	37.15493052073698	40.0	35.0	41.0	32.0	41.0
35	37.106667004545066	39.0	35.0	41.0	32.0	41.0
36	37.046786504895316	39.0	35.0	41.0	32.0	41.0
37	36.9775606506814	39.0	35.0	41.0	32.0	41.0
38	36.843340869228385	39.0	35.0	41.0	31.0	41.0
39	36.79195137447123	39.0	35.0	41.0	31.0	41.0
40	36.659771533865374	39.0	35.0	41.0	31.0	41.0
41	36.568404990705325	39.0	35.0	41.0	31.0	41.0
42	36.567266461164195	39.0	35.0	41.0	31.0	41.0
43	36.455857091916805	39.0	35.0	41.0	31.0	41.0
44	36.30697580126958	38.0	35.0	41.0	31.0	41.0
45	36.287294987754926	38.0	35.0	41.0	31.0	41.0
46	36.21067502674368	38.0	35.0	40.0	31.0	41.0
47	36.10981832399039	38.0	35.0	40.0	30.0	41.0
48	36.053147065797596	38.0	35.0	40.0	30.0	41.0
49	36.00113671947827	38.0	35.0	40.0	30.0	41.0
50	35.86740384493554	38.0	35.0	40.0	30.0	41.0
51	34.69461886411315	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	21.0
11	16.0
12	13.0
13	12.0
14	25.0
15	22.0
16	41.0
17	78.0
18	138.0
19	261.0
20	471.0
21	894.0
22	1214.0
23	1700.0
24	2359.0
25	3098.0
26	3973.0
27	4807.0
28	5574.0
29	6502.0
30	8075.0
31	10029.0
32	13553.0
33	19792.0
34	36556.0
35	47895.0
36	41052.0
37	62594.0
38	103267.0
39	178262.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06036921662289	17.810475557816122	21.60780643911763	16.521348786443355
2	29.178756378209016	25.991597688187714	26.56755969134808	18.262086242255194
3	31.847694070415066	25.487857193280323	25.647504737839544	17.016943998465067
4	28.44368984934847	25.510120966501166	26.692454028928424	19.35373515522194
5	25.285854177715596	30.763104402615905	23.82278036516208	20.12826105450642
6	26.35433428602975	35.278306215574865	23.21984842533581	15.147511073059567
7	79.7021360551852	6.188061911390182	9.305533181167382	4.8042688522572385
8	80.8415706277479	6.159643924433496	7.828340878278703	5.1704445695399
9	75.09371600475686	6.969466049555901	10.443519703439302	7.4932982422479535
10	43.312089228858916	24.60219343417797	16.77457658104466	15.311140755918453
11	32.63796751661185	24.060984637996476	23.17568289146682	20.125364953924848
12	28.67537789587432	21.393313989794866	28.95810971515041	20.973198399180404
13	27.30534131450385	22.565510700186618	30.863562891539225	19.26558509377031
14	22.063037249283667	27.38226898620189	30.222800637866154	20.331893126648286
15	19.14431088191693	23.90930137003658	36.44036657393111	20.50602117411538
16	23.403569805979362	23.45895772960195	31.54396552192258	21.593506942496113
17	24.591876075856113	23.06255396249912	28.060680547435414	24.284889414209356
18	25.444234678270377	22.88951195275012	29.483027945560547	22.183225423418957
19	26.965773521314397	24.613415823931565	26.23505114332621	22.185759511427833
20	26.77752698351214	25.298343611473626	26.802686857314555	21.121442547699683
21	25.66198524074741	25.90417165188147	28.403687460065484	20.03015564730563
22	23.91364552090894	23.88251243965703	28.31807148662273	23.8857705528113
23	23.319944901686434	25.54559819862544	27.694866842725446	23.43959005696268
24	23.12663018786642	24.388425010000596	28.649131984353815	23.83581281777916
25	24.22696740257789	25.384864616348125	26.413704347952006	23.974463633121978
26	21.50535688104448	25.834303225351018	26.79381754928349	25.86652234432102
27	22.681535729735895	26.127533409235305	27.783740929322477	23.40718993170633
28	22.00746831937473	25.009457578461703	28.843713742178267	24.1393603599853
29	23.465473955910486	26.514524849448023	27.244704208577165	22.775296986064326
30	25.11932839427513	25.140325123491543	26.24772158337059	23.492624898862736
31	25.568767003278026	25.324227510421437	25.06357845807985	24.04342702822069
32	27.005594904311025	27.81994218659214	24.088497593521424	21.08596531557541
33	27.708985333060614	25.141411161209632	24.224433314569016	22.925170191160742
34	23.324832071417838	27.08541867659064	26.948396917824958	22.641352334166566
35	23.642860116531846	28.267751739017893	25.56062172039235	22.52876642405791
36	27.177550876341932	27.34697276036397	24.51114727214476	20.964329091149338
37	25.425048011917458	28.34630846729307	24.59549620158308	21.633147319206397
38	24.49956286981847	29.481036876410716	23.868574955608207	22.150825298162605
39	24.4689728074256	25.68424901396826	25.84606863396366	24.000709544642483
40	25.536185871735324	24.164882246360417	27.122343959005697	23.176587922898563
41	22.748508055684773	24.987193805240857	26.167173785945586	26.097124353128788
42	23.963965268513775	26.2196656089866	26.827484718544277	22.98888440395535
43	24.33068400465548	24.74663645068393	26.57425692394297	24.348422620717617
44	22.997391699413722	25.246575813578005	27.725637911404664	24.030394575603612
45	22.971869813038605	24.819943996655002	26.806850001900568	25.40133618840582
46	23.946226652451642	25.93186561369276	26.803772895032647	23.318134838822953
47	22.55754642358729	25.514465117373526	28.704519907976405	23.22346855106278
48	23.350353957792954	26.00553517223653	26.63091189156999	24.01319897840052
49	24.131577089672323	24.216831050542385	28.26594167615441	23.385650183630876
50	22.66904629597786	24.784466764530734	28.5486734954305	23.99781344406091
51	22.828512834250734	25.35735166082318	26.123913283508337	25.690222221417752
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	237.0
1	627.0
2	1017.0
3	1551.0
4	2085.0
5	1531.0
6	977.0
7	974.5
8	972.0
9	998.5
10	1025.0
11	1041.5
12	1058.0
13	1154.0
14	1250.0
15	1168.5
16	1087.0
17	1078.0
18	1069.0
19	1093.5
20	1118.0
21	1169.5
22	1221.0
23	1567.5
24	1914.0
25	2174.5
26	3228.5
27	4022.0
28	4266.0
29	4510.0
30	5869.5
31	7229.0
32	8391.5
33	9554.0
34	11217.5
35	12881.0
36	13634.0
37	14387.0
38	15801.0
39	17215.0
40	19744.0
41	22273.0
42	24868.0
43	27463.0
44	30288.0
45	33113.0
46	39966.0
47	46819.0
48	48763.5
49	50708.0
50	50717.5
51	50727.0
52	44750.5
53	38774.0
54	36152.0
55	33530.0
56	32274.5
57	31019.0
58	30333.0
59	29647.0
60	27595.0
61	25543.0
62	23477.5
63	21412.0
64	19042.5
65	16673.0
66	14825.0
67	12977.0
68	11110.5
69	9244.0
70	7741.0
71	6238.0
72	5066.5
73	3895.0
74	3163.5
75	1791.5
76	1151.0
77	961.0
78	771.0
79	600.0
80	429.0
81	308.0
82	187.0
83	149.0
84	111.0
85	83.5
86	56.0
87	30.5
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	552467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.211366933330613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43370870349071	22.48744085849802
2	9.444539743151829	5.706649113935679
3	3.2707883128520923	2.9644495764247174
4	1.7892969306625615	2.162284244997155
5	1.1061384684009712	1.6708977573967034
6	0.8239730631768503	1.493601153288974
7	0.6345862321844766	1.3420202257955474
8	0.5358891980336867	1.2951956157923186
9	0.48262720324671865	1.3122744776364375
>10	7.1075929304979875	50.4838411544472
>50	0.3391055174393944	6.015495635838269
>100	0.027559812371399543	1.5416132975397043
>500	0.00119825271179998	0.2930458998141107
>1k	0.00299563177949995	1.2311909885951706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	1624	0.2939542090296796	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	1449	0.2622781089187227	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	1414	0.2559428888965314	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1252	0.22661987050810276	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1063	0.19240968238826936	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	955	0.17286100346265026	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	664	0.12018817413528772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010860377180899492	0.0	0.0	0.13231559532062548	0.0
2	0.0010860377180899492	0.0	0.0	0.7026664036041972	0.0
3	0.0010860377180899492	0.0	0.0	0.9506450159014023	0.0
4	0.0010860377180899492	0.0	0.0	1.2858686582185	0.0
5	0.0010860377180899492	0.0	0.0	2.307649144654794	0.0
6	0.0010860377180899492	0.0	0.0	3.1509574327516394	0.0
7	0.0010860377180899492	0.0	0.0	3.8328081134257794	0.0
8	0.0010860377180899492	0.0	0.0	5.036318911355791	0.0
9	0.0010860377180899492	0.0	0.0	5.598162424180992	0.0
10	0.0010860377180899492	0.0	0.0	6.702481777192122	0.0
11	0.0010860377180899492	0.0	0.0	7.612400378665151	0.0
12	0.0010860377180899492	0.0	0.0	8.773917718162352	0.0
13	0.0010860377180899492	0.0	0.0	9.140817460590407	0.0
14	0.0010860377180899492	0.0	0.0	9.310601357185135	0.0
15	0.0010860377180899492	0.0	0.0	9.58247279928032	0.0
16	0.0010860377180899492	0.0	0.0	10.059424363808155	0.0
17	0.0010860377180899492	0.0	0.0	10.612941587461332	0.0
18	0.0010860377180899492	0.0	0.0	11.339862833436205	0.0
19	0.0010860377180899492	0.0	0.0	11.753281191455779	0.0
20	0.0010860377180899492	0.0	0.0	12.160726342025859	0.0
21	0.0010860377180899492	0.0	0.0	12.613423064183019	0.0
22	0.0010860377180899492	0.0	0.0	13.102321043609845	0.0
23	0.0010860377180899492	0.0	0.0	13.576919526415152	0.0
24	0.0010860377180899492	0.0	0.0	13.954136627165061	0.0
25	0.0010860377180899492	0.0	0.0	14.270716621988282	0.0
26	0.0010860377180899492	0.0	0.0	14.594898880838132	0.0
27	0.0010860377180899492	0.0	0.0	14.98080428333276	0.0
28	0.0012670440044382741	0.0	0.0	15.306615598759745	0.0
29	0.0012670440044382741	0.0	0.0	15.682384649218868	0.0
30	0.0012670440044382741	0.0	0.0	16.13471935880333	0.0
31	0.0012670440044382741	0.0	0.0	16.489491680046047	0.0
32	0.0012670440044382741	0.0	0.0	16.86888085623214	0.0
33	0.0012670440044382741	0.0	0.0	17.218403995170753	0.0
34	0.0012670440044382741	0.0	0.0	17.58258864330358	0.0
35	0.0012670440044382741	0.0	0.0	17.93953303998248	0.0
36	0.0012670440044382741	0.0	0.0	18.272946619436095	0.0
37	0.0012670440044382741	0.0	0.0	18.653240827053924	0.0
38	0.001448050290786599	0.0	0.0	19.040775286125687	0.0
39	0.001448050290786599	0.0	0.0	19.67140118776325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	25	3.888165E-5	45.000004	5
TATACGA	25	3.888165E-5	45.000004	13
GTACGAG	35	1.2103919E-7	45.000004	1
ACCGGTA	20	7.0301036E-4	45.0	41
CCAATCG	20	7.0301036E-4	45.0	12
TACGCGT	20	7.0301036E-4	45.0	21
TACGCGG	40	6.8048394E-9	45.0	2
ATCGGTT	20	7.0301036E-4	45.0	30
CGTAAGG	90	0.0	40.0	2
TAGGGAT	245	0.0	39.489796	5
TAACGCG	40	3.453879E-7	39.375	1
CCGAACG	40	3.453879E-7	39.375	1
TATTGCG	35	6.2423987E-6	38.57143	1
TAGCACG	35	6.2423987E-6	38.57143	1
TTCGGTG	35	6.2423987E-6	38.57143	34
TCACGAC	65	9.094947E-12	38.07692	25
GGCGATA	125	0.0	37.8	8
CGTTTTT	585	0.0	37.69231	1
CTATACG	30	1.13915434E-4	37.500004	12
TAACGTG	30	1.13915434E-4	37.500004	42
>>END_MODULE