##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935749.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334394
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86899585518879	33.0	31.0	34.0	30.0	34.0
2	31.92595560925136	33.0	31.0	34.0	30.0	34.0
3	31.99161169159734	33.0	31.0	34.0	30.0	34.0
4	35.66926141019277	37.0	35.0	37.0	33.0	37.0
5	34.70711795068093	37.0	35.0	37.0	33.0	37.0
6	35.17244926643421	37.0	35.0	37.0	33.0	37.0
7	35.725306076065955	37.0	35.0	37.0	33.0	37.0
8	35.67015556499219	37.0	35.0	37.0	35.0	37.0
9	37.49537671130463	39.0	37.0	39.0	35.0	39.0
10	37.14357614072023	39.0	37.0	39.0	34.0	39.0
11	37.13650962636889	39.0	37.0	39.0	34.0	39.0
12	36.96960770827228	39.0	35.0	39.0	33.0	39.0
13	36.72477376986429	39.0	35.0	39.0	33.0	39.0
14	37.807039001895966	40.0	36.0	41.0	33.0	41.0
15	37.88566481456007	40.0	36.0	41.0	33.0	41.0
16	38.04390629018463	40.0	36.0	41.0	33.0	41.0
17	37.9578281906972	40.0	36.0	41.0	33.0	41.0
18	38.00009270501265	40.0	36.0	41.0	33.0	41.0
19	37.90214238293749	40.0	36.0	41.0	33.0	41.0
20	37.784063111180224	40.0	36.0	41.0	33.0	41.0
21	37.81364199118405	40.0	36.0	41.0	33.0	41.0
22	37.87674719044002	40.0	36.0	41.0	33.0	41.0
23	37.82604352948916	40.0	36.0	41.0	34.0	41.0
24	37.83162975412238	40.0	36.0	41.0	33.0	41.0
25	37.68718338247696	39.0	35.0	41.0	33.0	41.0
26	37.66140540799177	40.0	35.0	41.0	33.0	41.0
27	37.60383858562056	40.0	35.0	41.0	33.0	41.0
28	37.458133220093664	39.0	35.0	41.0	33.0	41.0
29	37.4606512078566	39.0	36.0	41.0	33.0	41.0
30	37.373684934538296	39.0	36.0	41.0	33.0	41.0
31	37.24083865141121	39.0	35.0	41.0	32.0	41.0
32	37.15574741173586	39.0	35.0	41.0	32.0	41.0
33	36.98007141276458	39.0	35.0	41.0	32.0	41.0
34	36.858627846193414	39.0	35.0	41.0	31.0	41.0
35	36.792606924765394	39.0	35.0	41.0	31.0	41.0
36	36.73046466144727	39.0	35.0	41.0	31.0	41.0
37	36.60891044695778	39.0	35.0	41.0	31.0	41.0
38	36.550488346082766	39.0	35.0	41.0	31.0	41.0
39	36.505032985041595	39.0	35.0	41.0	30.0	41.0
40	36.38824261200859	39.0	35.0	41.0	30.0	41.0
41	36.26275591069218	39.0	35.0	41.0	30.0	41.0
42	36.358065635148954	39.0	35.0	41.0	30.0	41.0
43	36.225473543185586	39.0	35.0	41.0	30.0	41.0
44	36.12528334838544	39.0	35.0	40.0	30.0	41.0
45	36.09957415503867	39.0	35.0	40.0	29.0	41.0
46	35.99011046848927	38.0	35.0	40.0	29.0	41.0
47	35.75661046549878	38.0	35.0	40.0	28.0	41.0
48	35.80125540530033	38.0	35.0	40.0	28.0	41.0
49	35.7961416771832	38.0	35.0	40.0	28.0	41.0
50	35.63786730623157	38.0	35.0	40.0	28.0	41.0
51	34.447451210248985	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	6.0
11	5.0
12	3.0
13	8.0
14	6.0
15	6.0
16	13.0
17	40.0
18	103.0
19	237.0
20	475.0
21	862.0
22	1226.0
23	1423.0
24	1860.0
25	2497.0
26	2983.0
27	3235.0
28	3762.0
29	4323.0
30	5407.0
31	7067.0
32	9458.0
33	12713.0
34	22045.0
35	32265.0
36	24287.0
37	35231.0
38	62617.0
39	100156.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.265728452065524	18.365461102770983	22.590118243748393	15.778692201415097
2	25.37545530123148	30.970352338857754	25.763620160648813	17.890572199261946
3	27.528903030556766	29.85699504177707	27.383565494596198	15.230536433069972
4	26.007942726245087	24.24565034061616	32.44316584627715	17.303241086861608
5	28.035790115851363	28.65452131318146	25.341064732022705	17.968623838944477
6	21.362823495636885	37.748883054121784	25.744481061263063	15.14381238897827
7	74.17537396005909	5.767447980525967	14.948533765557995	5.108644293856948
8	74.99057997452108	9.289341315932703	9.450827467000005	6.269251242546218
9	70.28266057405335	5.840714845362058	11.623115247283145	12.253509333301436
10	38.909490002811054	25.17718619353218	19.636715969784145	16.276607833872617
11	31.392608719055964	26.045323779732886	24.751640280627043	17.810427220584103
12	27.779505613138994	20.87597265501175	30.73679551666597	20.607726215183288
13	24.191522575165823	26.148196438931322	32.61541774074894	17.04486324515392
14	18.267971315274796	34.40851211445182	26.697548400988058	20.625968169285333
15	14.6147957200189	25.636524578790286	41.042303390611075	18.706376310579735
16	16.747011010963114	25.141001333755987	33.25926900602283	24.85271864925806
17	17.516163567528125	25.257032123782125	30.069020377159877	27.15778393152987
18	20.101138178316596	25.20290435833179	31.667434224298287	23.028523239053335
19	20.81496677571966	28.135373242342865	27.60097370168125	23.448686280256226
20	22.917277223873633	26.368894178723302	30.52716256870638	20.18666602869669
21	20.661853980633623	30.944335125630246	28.592319240177755	19.80149165355838
22	17.938419947726338	24.970244681423708	29.304652595441304	27.78668277540865
23	17.037087986028457	30.392291727722387	28.461934125612302	24.108686160636854
24	20.864010717895656	24.586864596852813	29.242151474009702	25.30697321124183
25	16.770635836767404	31.27209220261129	27.919759325825222	24.03751263479608
26	18.564029258898184	26.83152209668834	29.787615806503702	24.81683283790977
27	20.828124906547366	28.788195960453837	28.35756622427436	22.02611290872444
28	16.22905913383613	28.7588892145194	33.35526355137951	21.656788100264958
29	21.3646177862043	26.285758715766434	29.380311847700618	22.969311650328656
30	21.080222731269103	27.889256386179177	31.219459679300464	19.811061203251253
31	22.57516582235327	29.34622032691974	25.272881690460952	22.805732160266036
32	24.60779798680598	30.291213359091373	23.876026483728772	21.22496217037387
33	24.38560500487449	26.91076993008248	25.454105037769814	23.249520027273217
34	22.8389265357632	25.7863478411694	29.342631745784914	22.032093877282488
35	20.506348798124368	25.23011776527091	28.071376878771748	26.192156557832973
36	27.044743625782758	25.67719516498502	27.632373786611	19.64568742262122
37	19.21475863801384	28.211630591457983	33.17762878520548	19.395981985322702
38	21.353552994371906	29.952690538705838	24.760910781892022	23.932845685030234
39	23.186420808985808	27.19127735545494	28.86146282528993	20.760839010269322
40	24.80905757878431	22.870326620692953	28.998726053697137	23.3218897468256
41	21.611930836079594	24.120947146180853	28.231068739271635	26.036053278467914
42	22.6283964425199	22.968414505044947	28.578563012494246	25.824626039940906
43	26.069845750820885	23.783321471079027	27.290262385090642	22.85657039300944
44	22.222587725856325	25.773787807197497	28.806138866127977	23.197485600818197
45	20.201618450091807	26.06386478226284	27.378182622893952	26.356334144751404
46	26.166139344605465	25.90656530918617	27.7062387483029	20.221056597905466
47	19.89718715048715	24.1888311393147	33.3044253186361	22.609556391562048
48	21.995909017506296	24.056053637326027	28.831557982499685	25.11647936266799
49	22.10536074211858	20.759044719701908	33.51017063703296	23.625423901146554
50	21.870906774643085	22.217503902581985	31.049600172251896	24.861989150523037
51	19.8663851624132	22.189094301931256	27.252283234747033	30.69223730090851
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	260.5
2	372.0
3	878.0
4	1384.0
5	945.0
6	506.0
7	563.0
8	620.0
9	641.5
10	663.0
11	721.0
12	779.0
13	748.0
14	717.0
15	752.0
16	787.0
17	751.5
18	716.0
19	731.0
20	746.0
21	787.0
22	828.0
23	1030.0
24	1232.0
25	1481.5
26	2194.5
27	2658.0
28	2808.5
29	2959.0
30	3462.0
31	3965.0
32	4851.5
33	5738.0
34	7205.0
35	8672.0
36	9025.5
37	9379.0
38	10335.0
39	11291.0
40	13235.5
41	15180.0
42	17729.0
43	20278.0
44	24781.5
45	29285.0
46	40752.5
47	52220.0
48	46777.0
49	41334.0
50	40049.5
51	38765.0
52	31935.5
53	25106.0
54	21088.0
55	17070.0
56	14215.0
57	11360.0
58	10040.0
59	8720.0
60	7960.0
61	7200.0
62	6281.0
63	5362.0
64	4157.5
65	2953.0
66	2264.5
67	1576.0
68	1269.5
69	963.0
70	663.5
71	364.0
72	358.0
73	352.0
74	263.0
75	135.0
76	96.0
77	79.5
78	63.0
79	52.5
80	42.0
81	35.5
82	29.0
83	17.5
84	6.0
85	4.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.375409162830387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43325768789984	22.41247687791757
2	12.09643196537832	7.590610046481706
3	4.632107372427389	4.360027922882175
4	2.261836593454006	2.838641943163276
5	1.3854112921562654	2.1733923075104187
6	0.8829910906964114	1.6622524054640269
7	0.6597416027437153	1.4489763909477897
8	0.5730369600706579	1.438341527011311
9	0.4125864425637686	1.1650561605427372
>10	5.195200378591036	36.396589223673175
>50	0.4006273834445121	7.74921753488422
>100	0.05381561867165087	2.8419738600880713
>500	0.005979513185738986	1.4811783667331053
>1k	0.005979513185738986	4.805621768697719
>5k	9.965855309564977E-4	1.6356436640027179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGC	5231	1.56432232635753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCG	4626	1.3833980274765696	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC	4519	1.3513998456910112	No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	2555	0.7640687332906692	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	1576	0.47130032237420527	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	1058	0.31639323672075453	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCT	1035	0.309515122878999	No Hit
GAACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCT	966	0.2888807813537324	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTAAGTCGT	938	0.2805074253724648	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTA	855	0.2556864058565644	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.23385587061968816	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTC	674	0.2015586404062274	No Hit
TCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	522	0.15610327936506038	No Hit
GCCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG	428	0.12799272714223342	No Hit
ACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	378	0.11304030574711268	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	374	0.11184411203550304	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCC	335	0.10018122334730886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34360664365987426	0.0
2	0.0	0.0	0.0	1.9683367524536923	0.0
3	0.0	0.0	0.0	2.5694240925375453	0.0
4	0.0	0.0	0.0	3.4692608120959108	0.0
5	0.0	0.0	0.0	6.324575201708164	0.0
6	0.0	0.0	0.0	7.590447197019086	0.0
7	0.0	0.0	0.0	8.995675759732531	0.0
8	0.0	0.0	0.0	11.121311985262894	0.0
9	0.0	0.0	0.0	11.919173190906536	0.0
10	0.0	0.0	0.0	14.863903060461611	0.0
11	0.0	0.0	0.0	16.639054528490345	0.0
12	0.0	0.0	0.0	19.883729971231542	0.0
13	0.0	0.0	0.0	20.674414014605524	0.0
14	0.0	0.0	0.0	21.018917803549108	0.0
15	0.0	0.0	0.0	21.67203957008798	0.0
16	0.0	0.0	0.0	22.633779314222146	0.0
17	0.0	0.0	0.0	23.72171749493113	0.0
18	0.0	0.0	0.0	25.24387399295442	0.0
19	0.0	0.0	0.0	26.237910967302046	0.0
20	0.0	0.0	0.0	27.068069403159146	0.0
21	0.0	0.0	0.0	27.875201110067763	0.0
22	0.0	0.0	0.0	28.749618713254424	0.0
23	0.0	0.0	0.0	29.580973342823135	0.0
24	0.0	0.0	0.0	30.203293121288063	0.0
25	0.0	0.0	0.0	30.69373254304802	0.0
26	0.0	0.0	0.0	31.20749774218437	0.0
27	0.0	0.0	0.0	31.749074445115642	0.0
28	2.990484279024145E-4	0.0	0.0	32.251475803991696	0.0
29	2.990484279024145E-4	0.0	0.0	32.767035293695464	0.0
30	2.990484279024145E-4	0.0	0.0	33.511964927600374	0.0
31	2.990484279024145E-4	0.0	0.0	34.079857892187064	0.0
32	2.990484279024145E-4	0.0	0.0	34.57508208879346	0.0
33	2.990484279024145E-4	0.0	0.0	35.09961303133429	0.0
34	2.990484279024145E-4	0.0	0.0	35.59782771221972	0.0
35	2.990484279024145E-4	0.0	0.0	36.14927301327177	0.0
36	2.990484279024145E-4	0.0	0.0	36.63104003062256	0.0
37	2.990484279024145E-4	0.0	0.0	37.114900386968664	0.0
38	2.990484279024145E-4	0.0	0.0	37.58769595148238	0.0
39	2.990484279024145E-4	0.0	0.0	38.079331566953954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCC	35	1.208482E-7	45.000004	16
AGCCCGA	30	2.1608685E-6	45.000004	25
TAACCCG	30	2.1608685E-6	45.000004	37
GTGCTAG	30	2.1608685E-6	45.000004	1
GACGGTC	60	0.0	45.000004	29
CGGTCTA	60	0.0	45.000004	31
TCGAGTG	30	2.1608685E-6	45.000004	2
ACCCGTA	30	2.1608685E-6	45.000004	39
CGGTATT	30	2.1608685E-6	45.000004	28
GGTTAGC	30	2.1608685E-6	45.000004	15
ATCGAAT	30	2.1608685E-6	45.000004	11
CTTCGCA	20	7.026105E-4	45.0	40
AACCGTT	20	7.026105E-4	45.0	32
AAGTACC	20	7.026105E-4	45.0	41
CTGTCGG	25	3.8848528E-5	45.0	2
ACCGGAA	20	7.026105E-4	45.0	35
CAAAATC	25	3.8848528E-5	45.0	13
TTGTGCG	20	7.026105E-4	45.0	1
GATCGAA	40	6.7902874E-9	45.0	10
ACGTTAG	25	3.8848528E-5	45.0	1
>>END_MODULE