SRR2935748.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469343
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	5836	1.2434402984597617	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	5080	1.0823640706263862	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC	5007	1.0668104137059677	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2048	0.4363546489454408	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1159	0.24694093658582314	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	1106	0.23564855553401243	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1014	0.21604668653841647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT	920	0.1960186899559597	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.18877452097932643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA	853	0.18174341579612352	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	734	0.156388824377907	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC	698	0.14871852781441292	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTC	20	7.0290145E-4	45.000004	20
CACGCTC	20	7.0290145E-4	45.000004	34
TCGGGTA	20	7.0290145E-4	45.000004	5
ATAGCGG	60	0.0	45.000004	2
TAAACGT	20	7.0290145E-4	45.000004	15
TTCCGTA	20	7.0290145E-4	45.000004	24
TCGATCT	20	7.0290145E-4	45.000004	14
CCGTCCG	20	7.0290145E-4	45.000004	44
CCGTAAC	20	7.0290145E-4	45.000004	26
TCGTGCG	20	7.0290145E-4	45.000004	1
GCGATCT	30	2.1627457E-6	45.000004	9
AGTTATG	20	7.0290145E-4	45.000004	1
TTACGAG	20	7.0290145E-4	45.000004	1
GCTAACG	40	6.7993824E-9	45.000004	1
ACGCGGC	20	7.0290145E-4	45.000004	14
CTTAACG	35	1.2098826E-7	45.0	1
GCCGCCG	25	3.8872662E-5	45.0	16
TCTACGG	35	1.2098826E-7	45.0	2
GTCGTTG	25	3.8872662E-5	45.0	1
CGTAATA	25	3.8872662E-5	45.0	24