##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935748.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469343 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.072837988422116 33.0 31.0 34.0 30.0 34.0 2 32.11187127537856 33.0 31.0 34.0 30.0 34.0 3 32.183671643126665 34.0 31.0 34.0 30.0 34.0 4 35.81407414193884 37.0 35.0 37.0 35.0 37.0 5 34.80701320782455 37.0 35.0 37.0 33.0 37.0 6 35.31067683975259 37.0 35.0 37.0 33.0 37.0 7 35.86759150557268 37.0 35.0 37.0 35.0 37.0 8 35.84051535870355 37.0 35.0 37.0 35.0 37.0 9 37.71538725409775 39.0 38.0 39.0 35.0 39.0 10 37.32184138252834 39.0 37.0 39.0 34.0 39.0 11 37.309287663819426 39.0 37.0 39.0 34.0 39.0 12 37.23084183635422 39.0 37.0 39.0 34.0 39.0 13 37.08840442917014 39.0 37.0 39.0 33.0 39.0 14 38.26392425156016 40.0 37.0 41.0 33.0 41.0 15 38.30207332377387 40.0 37.0 41.0 33.0 41.0 16 38.396132466021655 40.0 37.0 41.0 34.0 41.0 17 38.318521848626695 40.0 37.0 41.0 34.0 41.0 18 38.34407245873487 40.0 37.0 41.0 34.0 41.0 19 38.267718917721155 40.0 37.0 41.0 34.0 41.0 20 38.16604700613411 40.0 37.0 41.0 34.0 41.0 21 38.220778833390504 40.0 37.0 41.0 34.0 41.0 22 38.23766413901986 40.0 37.0 41.0 34.0 41.0 23 38.198635113339286 40.0 37.0 41.0 34.0 41.0 24 38.18347562443671 40.0 37.0 41.0 34.0 41.0 25 38.08863879934291 40.0 37.0 41.0 34.0 41.0 26 38.04356941511858 40.0 37.0 41.0 34.0 41.0 27 38.03218328599766 40.0 37.0 41.0 34.0 41.0 28 37.946322838521084 40.0 36.0 41.0 34.0 41.0 29 37.88723385668903 40.0 36.0 41.0 33.0 41.0 30 37.82830254206412 40.0 36.0 41.0 33.0 41.0 31 37.81617495094206 40.0 36.0 41.0 33.0 41.0 32 37.6789064713866 40.0 36.0 41.0 33.0 41.0 33 37.561663857775656 40.0 36.0 41.0 33.0 41.0 34 37.448330964774165 40.0 36.0 41.0 33.0 41.0 35 37.4105760605783 40.0 36.0 41.0 33.0 41.0 36 37.3808003954464 40.0 36.0 41.0 33.0 41.0 37 37.31804671636735 40.0 36.0 41.0 33.0 41.0 38 37.23892973795284 40.0 36.0 41.0 32.0 41.0 39 37.16703349149769 40.0 35.0 41.0 32.0 41.0 40 37.02318560200109 40.0 35.0 41.0 31.0 41.0 41 36.92724510645733 40.0 35.0 41.0 31.0 41.0 42 36.94689811076334 40.0 35.0 41.0 31.0 41.0 43 36.87006091493854 39.0 35.0 41.0 31.0 41.0 44 36.75460377591655 39.0 35.0 41.0 31.0 41.0 45 36.72571445616532 39.0 35.0 41.0 31.0 41.0 46 36.537698442290605 39.0 35.0 41.0 31.0 41.0 47 36.360030936862806 39.0 35.0 41.0 30.0 41.0 48 36.35887613110241 39.0 35.0 41.0 30.0 41.0 49 36.3543037820954 39.0 35.0 41.0 30.0 41.0 50 36.23746812032991 39.0 35.0 41.0 30.0 41.0 51 34.992596033178295 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 6.0 10 9.0 11 11.0 12 8.0 13 3.0 14 3.0 15 12.0 16 20.0 17 38.0 18 103.0 19 236.0 20 553.0 21 977.0 22 1466.0 23 1841.0 24 2114.0 25 2582.0 26 3164.0 27 3830.0 28 4272.0 29 5031.0 30 6314.0 31 8426.0 32 11143.0 33 15334.0 34 26246.0 35 38672.0 36 31057.0 37 47038.0 38 86668.0 39 172006.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11495004719363 18.114257589864984 23.25804369086148 15.512748672079907 2 25.458992676997422 30.370965370741654 26.800016192848304 17.370025759412624 3 29.296910788059055 28.68925284919558 26.38582017841962 15.62801618432575 4 27.216768972798143 24.148224219813656 31.298858191131007 17.336148616257194 5 27.02117641042905 29.945050847674302 24.673000343032708 18.360772398863944 6 23.281906835725685 37.65178131984497 24.335294230445538 14.731017613983802 7 77.88568275227286 5.2518094442657075 12.359617593103552 4.502890210357884 8 80.21340469549988 7.924055541469671 7.233728850755204 4.628810912275244 9 75.51960932622836 5.638094101755006 8.732206509951144 10.110090062065483 10 38.23003645521506 26.03810006754122 18.084854786371586 17.647008690872134 11 30.069480103037648 27.26896960218859 24.377906989131613 18.28364330564214 12 26.99113441555536 22.876872564414512 28.71290293026635 21.419090089763777 13 24.079404614535637 24.600132525679513 32.00708224049363 19.313380619291223 14 19.297613898577374 30.89723294051472 27.543821895713794 22.261331265194112 15 16.861442484494283 26.574807763192375 36.30479201777804 20.258957734535297 16 18.90877247556691 25.103602269555523 31.335505163601034 24.65212009127653 17 19.846892358040922 24.749490244874217 30.15619706696382 25.247420330121038 18 20.619887800606378 25.283215047417347 30.180273275621456 23.91662387635482 19 21.859492950784396 27.504192030135744 27.106188864007773 23.53012615507209 20 24.47953841859791 26.23838003336579 29.10920158604688 20.17287996198942 21 23.212021911480516 29.569845507443382 27.414279109308122 19.803853471767983 22 20.355262569165834 25.391238390686553 29.00181743415796 25.251681605989653 23 19.79128270795559 29.204867229297122 28.119094137975853 22.884755924771436 24 21.183228470436333 24.895225879580604 29.68106480761405 24.240480842369013 25 19.065374363738247 30.405694769070806 27.29538951257396 23.233541354616985 26 19.14889537076296 28.00894015677234 28.947486166833215 23.89467830563149 27 21.984987525114892 28.498986881662237 26.960453229301386 22.55557236392148 28 18.584063254378993 28.828170442512192 30.65711004531867 21.930656257790147 29 19.727576633719902 27.50909249738464 29.605214097152828 23.158116771742627 30 21.22392365498154 28.308507850335467 28.752319732050974 21.71524876263202 31 23.05691146986319 28.422710043614156 24.792742194940587 23.727636291582062 32 23.16450868554554 30.35051124657234 26.11267239524186 20.372307672640268 33 22.016734030336025 26.225170078173104 25.348625632000477 26.40947025949039 34 22.27518041176709 28.52775049377534 26.828779804961407 22.36828928949617 35 22.09748520804614 26.202372252276053 26.75825568933594 24.94188685034186 36 21.21518803945089 29.84682843890289 25.648619453150467 23.28936406849575 37 22.30500934284734 26.1192773728382 29.989155052914395 21.586558231400062 38 19.364515929714514 28.323209252082165 26.36067865079483 25.951596167408486 39 21.51326428646001 25.06674223329207 28.943651018551463 24.476342461696458 40 21.63748047802993 26.001240031277767 28.34174580211061 24.0195336885817 41 20.850848952684924 26.491286756167664 27.02799445181882 25.629869839328595 42 20.981457058057753 28.065402062031392 28.103966608642295 22.84917427126856 43 21.837760443854496 26.36984039391234 25.646488815216163 26.145910347016997 44 22.067869340759316 25.861044055200566 28.221151695029008 23.849934909011104 45 20.29901372770021 25.408283494160987 27.397660133420548 26.895042644718252 46 23.70462540189158 27.25916866769079 26.458048804392526 22.578157126025104 47 20.212083699980614 25.93327268117347 31.122015242583785 22.732628376262138 48 20.790551899144123 26.413944599152433 27.514206028426972 25.281297473276474 49 20.24936986383093 24.29182921658574 30.988637307896354 24.470163611686978 50 20.86064988718272 23.686728043243427 29.98105862876404 25.471563440809813 51 19.76997632861255 24.59757576015835 26.994330372456815 28.638117538772285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 349.0 2 574.0 3 1133.5 4 1693.0 5 1172.5 6 652.0 7 648.5 8 645.0 9 677.5 10 710.0 11 727.5 12 745.0 13 732.5 14 720.0 15 685.0 16 650.0 17 700.5 18 751.0 19 818.0 20 885.0 21 963.0 22 1041.0 23 1525.0 24 2009.0 25 2279.0 26 2992.0 27 3435.0 28 4182.0 29 4929.0 30 6688.5 31 8448.0 32 9264.5 33 10081.0 34 11337.5 35 12594.0 36 13985.5 37 15377.0 38 16593.0 39 17809.0 40 19944.5 41 22080.0 42 24920.5 43 27761.0 44 31583.5 45 35406.0 46 48772.0 47 62138.0 48 55759.5 49 49381.0 50 47875.0 51 46369.0 52 40031.5 53 33694.0 54 29816.0 55 25938.0 56 23276.0 57 20614.0 58 19201.5 59 17789.0 60 16631.5 61 15474.0 62 13077.0 63 10680.0 64 8875.5 65 7071.0 66 5381.0 67 3691.0 68 2788.0 69 1885.0 70 1562.5 71 1240.0 72 1038.0 73 836.0 74 629.5 75 337.0 76 251.0 77 190.5 78 130.0 79 76.0 80 22.0 81 17.0 82 12.0 83 12.5 84 13.0 85 15.5 86 18.0 87 9.5 88 1.0 89 1.5 90 2.0 91 1.5 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 469343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.950606026927225 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75624622339005 23.137511249121115 2 9.720345288086396 6.017011549145209 3 3.350855933513182 3.1113306555347386 4 1.7322362107876226 2.144550420226599 5 1.1048368927147854 1.709768569521489 6 0.7819086018287371 1.4520327050560051 7 0.6270277400268134 1.3584821984667115 8 0.5251667998697314 1.3003384576952155 9 0.48640452952138646 1.3549063466636395 >10 6.607075923608077 46.02648824233845 >50 0.2617459184077012 4.98372712723055 >100 0.037884277664272534 2.001421494222544 >500 0.0034440252422065945 0.8721575769987672 >1k 0.002755220193765275 1.1356595973288763 >5k 0.0020664151453239567 3.3946138104501027 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC 5836 1.2434402984597617 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG 5080 1.0823640706263862 No Hit GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC 5007 1.0668104137059677 No Hit GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC 2048 0.4363546489454408 TruSeq Adapter, Index 21 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC 1159 0.24694093658582314 TruSeq Adapter, Index 21 (95% over 21bp) GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT 1106 0.23564855553401243 No Hit GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT 1014 0.21604668653841647 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT 920 0.1960186899559597 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.18877452097932643 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA 853 0.18174341579612352 No Hit CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT 734 0.156388824377907 TruSeq Adapter, Index 14 (95% over 23bp) GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC 698 0.14871852781441292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1306379343039099E-4 0.0 0.0 0.1704510347443128 0.0 2 2.1306379343039099E-4 0.0 0.0 1.0736284550957402 0.0 3 2.1306379343039099E-4 0.0 0.0 1.372130829691718 0.0 4 2.1306379343039099E-4 0.0 0.0 1.9684963875033823 0.0 5 2.1306379343039099E-4 0.0 0.0 3.9864235750826156 0.0 6 2.1306379343039099E-4 0.0 0.0 4.945849836899666 0.0 7 2.1306379343039099E-4 0.0 0.0 5.875447167636462 0.0 8 2.1306379343039099E-4 0.0 0.0 7.2002778351866334 0.0 9 2.1306379343039099E-4 0.0 0.0 7.679671370405013 0.0 10 2.1306379343039099E-4 0.0 0.0 9.790707435713326 0.0 11 2.1306379343039099E-4 0.0 0.0 10.872219251165992 0.0 12 2.1306379343039099E-4 0.0 0.0 13.202497959914178 0.0 13 2.1306379343039099E-4 0.0 0.0 13.68934872790262 0.0 14 2.1306379343039099E-4 0.0 0.0 13.898577373051266 0.0 15 2.1306379343039099E-4 0.0 0.0 14.341110872006187 0.0 16 2.1306379343039099E-4 0.0 0.0 14.937263366024421 0.0 17 2.1306379343039099E-4 0.0 0.0 15.66636766714322 0.0 18 2.1306379343039099E-4 0.0 0.0 16.55505674954138 0.0 19 2.1306379343039099E-4 0.0 0.0 17.244105057495265 0.0 20 2.1306379343039099E-4 0.0 0.0 17.739691441014354 0.0 21 2.1306379343039099E-4 0.0 0.0 18.29919696256256 0.0 22 2.1306379343039099E-4 0.0 0.0 18.926243706628203 0.0 23 2.1306379343039099E-4 0.0 0.0 19.517069605810676 0.0 24 2.1306379343039099E-4 0.0 0.0 19.97558288927288 0.0 25 2.1306379343039099E-4 0.0 0.0 20.405119496828547 0.0 26 2.1306379343039099E-4 0.0 0.0 20.796517685360175 0.0 27 2.1306379343039099E-4 0.0 0.0 21.218383996352348 0.0 28 2.1306379343039099E-4 0.0 0.0 21.598063676245303 0.0 29 2.1306379343039099E-4 0.0 0.0 22.030370113115566 0.0 30 2.1306379343039099E-4 0.0 0.0 22.60372478123675 0.0 31 2.1306379343039099E-4 0.0 0.0 23.064794830220116 0.0 32 2.1306379343039099E-4 0.0 0.0 23.504984627447303 0.0 33 2.1306379343039099E-4 0.0 0.0 23.925785619472325 0.0 34 2.1306379343039099E-4 0.0 0.0 24.35148707874625 0.0 35 2.1306379343039099E-4 0.0 0.0 24.79039849321285 0.0 36 2.1306379343039099E-4 0.0 0.0 25.225900886984572 0.0 37 2.1306379343039099E-4 0.0 0.0 25.636687880718366 0.0 38 2.1306379343039099E-4 0.0 0.0 26.04108296064925 0.0 39 2.1306379343039099E-4 0.0 0.0 26.47786373718155 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTC 20 7.0290145E-4 45.000004 20 CACGCTC 20 7.0290145E-4 45.000004 34 TCGGGTA 20 7.0290145E-4 45.000004 5 ATAGCGG 60 0.0 45.000004 2 TAAACGT 20 7.0290145E-4 45.000004 15 TTCCGTA 20 7.0290145E-4 45.000004 24 TCGATCT 20 7.0290145E-4 45.000004 14 CCGTCCG 20 7.0290145E-4 45.000004 44 CCGTAAC 20 7.0290145E-4 45.000004 26 TCGTGCG 20 7.0290145E-4 45.000004 1 GCGATCT 30 2.1627457E-6 45.000004 9 AGTTATG 20 7.0290145E-4 45.000004 1 TTACGAG 20 7.0290145E-4 45.000004 1 GCTAACG 40 6.7993824E-9 45.000004 1 ACGCGGC 20 7.0290145E-4 45.000004 14 CTTAACG 35 1.2098826E-7 45.0 1 GCCGCCG 25 3.8872662E-5 45.0 16 TCTACGG 35 1.2098826E-7 45.0 2 GTCGTTG 25 3.8872662E-5 45.0 1 CGTAATA 25 3.8872662E-5 45.0 24 >>END_MODULE