##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469343
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.072837988422116	33.0	31.0	34.0	30.0	34.0
2	32.11187127537856	33.0	31.0	34.0	30.0	34.0
3	32.183671643126665	34.0	31.0	34.0	30.0	34.0
4	35.81407414193884	37.0	35.0	37.0	35.0	37.0
5	34.80701320782455	37.0	35.0	37.0	33.0	37.0
6	35.31067683975259	37.0	35.0	37.0	33.0	37.0
7	35.86759150557268	37.0	35.0	37.0	35.0	37.0
8	35.84051535870355	37.0	35.0	37.0	35.0	37.0
9	37.71538725409775	39.0	38.0	39.0	35.0	39.0
10	37.32184138252834	39.0	37.0	39.0	34.0	39.0
11	37.309287663819426	39.0	37.0	39.0	34.0	39.0
12	37.23084183635422	39.0	37.0	39.0	34.0	39.0
13	37.08840442917014	39.0	37.0	39.0	33.0	39.0
14	38.26392425156016	40.0	37.0	41.0	33.0	41.0
15	38.30207332377387	40.0	37.0	41.0	33.0	41.0
16	38.396132466021655	40.0	37.0	41.0	34.0	41.0
17	38.318521848626695	40.0	37.0	41.0	34.0	41.0
18	38.34407245873487	40.0	37.0	41.0	34.0	41.0
19	38.267718917721155	40.0	37.0	41.0	34.0	41.0
20	38.16604700613411	40.0	37.0	41.0	34.0	41.0
21	38.220778833390504	40.0	37.0	41.0	34.0	41.0
22	38.23766413901986	40.0	37.0	41.0	34.0	41.0
23	38.198635113339286	40.0	37.0	41.0	34.0	41.0
24	38.18347562443671	40.0	37.0	41.0	34.0	41.0
25	38.08863879934291	40.0	37.0	41.0	34.0	41.0
26	38.04356941511858	40.0	37.0	41.0	34.0	41.0
27	38.03218328599766	40.0	37.0	41.0	34.0	41.0
28	37.946322838521084	40.0	36.0	41.0	34.0	41.0
29	37.88723385668903	40.0	36.0	41.0	33.0	41.0
30	37.82830254206412	40.0	36.0	41.0	33.0	41.0
31	37.81617495094206	40.0	36.0	41.0	33.0	41.0
32	37.6789064713866	40.0	36.0	41.0	33.0	41.0
33	37.561663857775656	40.0	36.0	41.0	33.0	41.0
34	37.448330964774165	40.0	36.0	41.0	33.0	41.0
35	37.4105760605783	40.0	36.0	41.0	33.0	41.0
36	37.3808003954464	40.0	36.0	41.0	33.0	41.0
37	37.31804671636735	40.0	36.0	41.0	33.0	41.0
38	37.23892973795284	40.0	36.0	41.0	32.0	41.0
39	37.16703349149769	40.0	35.0	41.0	32.0	41.0
40	37.02318560200109	40.0	35.0	41.0	31.0	41.0
41	36.92724510645733	40.0	35.0	41.0	31.0	41.0
42	36.94689811076334	40.0	35.0	41.0	31.0	41.0
43	36.87006091493854	39.0	35.0	41.0	31.0	41.0
44	36.75460377591655	39.0	35.0	41.0	31.0	41.0
45	36.72571445616532	39.0	35.0	41.0	31.0	41.0
46	36.537698442290605	39.0	35.0	41.0	31.0	41.0
47	36.360030936862806	39.0	35.0	41.0	30.0	41.0
48	36.35887613110241	39.0	35.0	41.0	30.0	41.0
49	36.3543037820954	39.0	35.0	41.0	30.0	41.0
50	36.23746812032991	39.0	35.0	41.0	30.0	41.0
51	34.992596033178295	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	9.0
11	11.0
12	8.0
13	3.0
14	3.0
15	12.0
16	20.0
17	38.0
18	103.0
19	236.0
20	553.0
21	977.0
22	1466.0
23	1841.0
24	2114.0
25	2582.0
26	3164.0
27	3830.0
28	4272.0
29	5031.0
30	6314.0
31	8426.0
32	11143.0
33	15334.0
34	26246.0
35	38672.0
36	31057.0
37	47038.0
38	86668.0
39	172006.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11495004719363	18.114257589864984	23.25804369086148	15.512748672079907
2	25.458992676997422	30.370965370741654	26.800016192848304	17.370025759412624
3	29.296910788059055	28.68925284919558	26.38582017841962	15.62801618432575
4	27.216768972798143	24.148224219813656	31.298858191131007	17.336148616257194
5	27.02117641042905	29.945050847674302	24.673000343032708	18.360772398863944
6	23.281906835725685	37.65178131984497	24.335294230445538	14.731017613983802
7	77.88568275227286	5.2518094442657075	12.359617593103552	4.502890210357884
8	80.21340469549988	7.924055541469671	7.233728850755204	4.628810912275244
9	75.51960932622836	5.638094101755006	8.732206509951144	10.110090062065483
10	38.23003645521506	26.03810006754122	18.084854786371586	17.647008690872134
11	30.069480103037648	27.26896960218859	24.377906989131613	18.28364330564214
12	26.99113441555536	22.876872564414512	28.71290293026635	21.419090089763777
13	24.079404614535637	24.600132525679513	32.00708224049363	19.313380619291223
14	19.297613898577374	30.89723294051472	27.543821895713794	22.261331265194112
15	16.861442484494283	26.574807763192375	36.30479201777804	20.258957734535297
16	18.90877247556691	25.103602269555523	31.335505163601034	24.65212009127653
17	19.846892358040922	24.749490244874217	30.15619706696382	25.247420330121038
18	20.619887800606378	25.283215047417347	30.180273275621456	23.91662387635482
19	21.859492950784396	27.504192030135744	27.106188864007773	23.53012615507209
20	24.47953841859791	26.23838003336579	29.10920158604688	20.17287996198942
21	23.212021911480516	29.569845507443382	27.414279109308122	19.803853471767983
22	20.355262569165834	25.391238390686553	29.00181743415796	25.251681605989653
23	19.79128270795559	29.204867229297122	28.119094137975853	22.884755924771436
24	21.183228470436333	24.895225879580604	29.68106480761405	24.240480842369013
25	19.065374363738247	30.405694769070806	27.29538951257396	23.233541354616985
26	19.14889537076296	28.00894015677234	28.947486166833215	23.89467830563149
27	21.984987525114892	28.498986881662237	26.960453229301386	22.55557236392148
28	18.584063254378993	28.828170442512192	30.65711004531867	21.930656257790147
29	19.727576633719902	27.50909249738464	29.605214097152828	23.158116771742627
30	21.22392365498154	28.308507850335467	28.752319732050974	21.71524876263202
31	23.05691146986319	28.422710043614156	24.792742194940587	23.727636291582062
32	23.16450868554554	30.35051124657234	26.11267239524186	20.372307672640268
33	22.016734030336025	26.225170078173104	25.348625632000477	26.40947025949039
34	22.27518041176709	28.52775049377534	26.828779804961407	22.36828928949617
35	22.09748520804614	26.202372252276053	26.75825568933594	24.94188685034186
36	21.21518803945089	29.84682843890289	25.648619453150467	23.28936406849575
37	22.30500934284734	26.1192773728382	29.989155052914395	21.586558231400062
38	19.364515929714514	28.323209252082165	26.36067865079483	25.951596167408486
39	21.51326428646001	25.06674223329207	28.943651018551463	24.476342461696458
40	21.63748047802993	26.001240031277767	28.34174580211061	24.0195336885817
41	20.850848952684924	26.491286756167664	27.02799445181882	25.629869839328595
42	20.981457058057753	28.065402062031392	28.103966608642295	22.84917427126856
43	21.837760443854496	26.36984039391234	25.646488815216163	26.145910347016997
44	22.067869340759316	25.861044055200566	28.221151695029008	23.849934909011104
45	20.29901372770021	25.408283494160987	27.397660133420548	26.895042644718252
46	23.70462540189158	27.25916866769079	26.458048804392526	22.578157126025104
47	20.212083699980614	25.93327268117347	31.122015242583785	22.732628376262138
48	20.790551899144123	26.413944599152433	27.514206028426972	25.281297473276474
49	20.24936986383093	24.29182921658574	30.988637307896354	24.470163611686978
50	20.86064988718272	23.686728043243427	29.98105862876404	25.471563440809813
51	19.76997632861255	24.59757576015835	26.994330372456815	28.638117538772285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	349.0
2	574.0
3	1133.5
4	1693.0
5	1172.5
6	652.0
7	648.5
8	645.0
9	677.5
10	710.0
11	727.5
12	745.0
13	732.5
14	720.0
15	685.0
16	650.0
17	700.5
18	751.0
19	818.0
20	885.0
21	963.0
22	1041.0
23	1525.0
24	2009.0
25	2279.0
26	2992.0
27	3435.0
28	4182.0
29	4929.0
30	6688.5
31	8448.0
32	9264.5
33	10081.0
34	11337.5
35	12594.0
36	13985.5
37	15377.0
38	16593.0
39	17809.0
40	19944.5
41	22080.0
42	24920.5
43	27761.0
44	31583.5
45	35406.0
46	48772.0
47	62138.0
48	55759.5
49	49381.0
50	47875.0
51	46369.0
52	40031.5
53	33694.0
54	29816.0
55	25938.0
56	23276.0
57	20614.0
58	19201.5
59	17789.0
60	16631.5
61	15474.0
62	13077.0
63	10680.0
64	8875.5
65	7071.0
66	5381.0
67	3691.0
68	2788.0
69	1885.0
70	1562.5
71	1240.0
72	1038.0
73	836.0
74	629.5
75	337.0
76	251.0
77	190.5
78	130.0
79	76.0
80	22.0
81	17.0
82	12.0
83	12.5
84	13.0
85	15.5
86	18.0
87	9.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469343.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.950606026927225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75624622339005	23.137511249121115
2	9.720345288086396	6.017011549145209
3	3.350855933513182	3.1113306555347386
4	1.7322362107876226	2.144550420226599
5	1.1048368927147854	1.709768569521489
6	0.7819086018287371	1.4520327050560051
7	0.6270277400268134	1.3584821984667115
8	0.5251667998697314	1.3003384576952155
9	0.48640452952138646	1.3549063466636395
>10	6.607075923608077	46.02648824233845
>50	0.2617459184077012	4.98372712723055
>100	0.037884277664272534	2.001421494222544
>500	0.0034440252422065945	0.8721575769987672
>1k	0.002755220193765275	1.1356595973288763
>5k	0.0020664151453239567	3.3946138104501027
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	5836	1.2434402984597617	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	5080	1.0823640706263862	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC	5007	1.0668104137059677	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2048	0.4363546489454408	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1159	0.24694093658582314	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	1106	0.23564855553401243	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1014	0.21604668653841647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT	920	0.1960186899559597	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.18877452097932643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA	853	0.18174341579612352	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	734	0.156388824377907	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC	698	0.14871852781441292	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1306379343039099E-4	0.0	0.0	0.1704510347443128	0.0
2	2.1306379343039099E-4	0.0	0.0	1.0736284550957402	0.0
3	2.1306379343039099E-4	0.0	0.0	1.372130829691718	0.0
4	2.1306379343039099E-4	0.0	0.0	1.9684963875033823	0.0
5	2.1306379343039099E-4	0.0	0.0	3.9864235750826156	0.0
6	2.1306379343039099E-4	0.0	0.0	4.945849836899666	0.0
7	2.1306379343039099E-4	0.0	0.0	5.875447167636462	0.0
8	2.1306379343039099E-4	0.0	0.0	7.2002778351866334	0.0
9	2.1306379343039099E-4	0.0	0.0	7.679671370405013	0.0
10	2.1306379343039099E-4	0.0	0.0	9.790707435713326	0.0
11	2.1306379343039099E-4	0.0	0.0	10.872219251165992	0.0
12	2.1306379343039099E-4	0.0	0.0	13.202497959914178	0.0
13	2.1306379343039099E-4	0.0	0.0	13.68934872790262	0.0
14	2.1306379343039099E-4	0.0	0.0	13.898577373051266	0.0
15	2.1306379343039099E-4	0.0	0.0	14.341110872006187	0.0
16	2.1306379343039099E-4	0.0	0.0	14.937263366024421	0.0
17	2.1306379343039099E-4	0.0	0.0	15.66636766714322	0.0
18	2.1306379343039099E-4	0.0	0.0	16.55505674954138	0.0
19	2.1306379343039099E-4	0.0	0.0	17.244105057495265	0.0
20	2.1306379343039099E-4	0.0	0.0	17.739691441014354	0.0
21	2.1306379343039099E-4	0.0	0.0	18.29919696256256	0.0
22	2.1306379343039099E-4	0.0	0.0	18.926243706628203	0.0
23	2.1306379343039099E-4	0.0	0.0	19.517069605810676	0.0
24	2.1306379343039099E-4	0.0	0.0	19.97558288927288	0.0
25	2.1306379343039099E-4	0.0	0.0	20.405119496828547	0.0
26	2.1306379343039099E-4	0.0	0.0	20.796517685360175	0.0
27	2.1306379343039099E-4	0.0	0.0	21.218383996352348	0.0
28	2.1306379343039099E-4	0.0	0.0	21.598063676245303	0.0
29	2.1306379343039099E-4	0.0	0.0	22.030370113115566	0.0
30	2.1306379343039099E-4	0.0	0.0	22.60372478123675	0.0
31	2.1306379343039099E-4	0.0	0.0	23.064794830220116	0.0
32	2.1306379343039099E-4	0.0	0.0	23.504984627447303	0.0
33	2.1306379343039099E-4	0.0	0.0	23.925785619472325	0.0
34	2.1306379343039099E-4	0.0	0.0	24.35148707874625	0.0
35	2.1306379343039099E-4	0.0	0.0	24.79039849321285	0.0
36	2.1306379343039099E-4	0.0	0.0	25.225900886984572	0.0
37	2.1306379343039099E-4	0.0	0.0	25.636687880718366	0.0
38	2.1306379343039099E-4	0.0	0.0	26.04108296064925	0.0
39	2.1306379343039099E-4	0.0	0.0	26.47786373718155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTC	20	7.0290145E-4	45.000004	20
CACGCTC	20	7.0290145E-4	45.000004	34
TCGGGTA	20	7.0290145E-4	45.000004	5
ATAGCGG	60	0.0	45.000004	2
TAAACGT	20	7.0290145E-4	45.000004	15
TTCCGTA	20	7.0290145E-4	45.000004	24
TCGATCT	20	7.0290145E-4	45.000004	14
CCGTCCG	20	7.0290145E-4	45.000004	44
CCGTAAC	20	7.0290145E-4	45.000004	26
TCGTGCG	20	7.0290145E-4	45.000004	1
GCGATCT	30	2.1627457E-6	45.000004	9
AGTTATG	20	7.0290145E-4	45.000004	1
TTACGAG	20	7.0290145E-4	45.000004	1
GCTAACG	40	6.7993824E-9	45.000004	1
ACGCGGC	20	7.0290145E-4	45.000004	14
CTTAACG	35	1.2098826E-7	45.0	1
GCCGCCG	25	3.8872662E-5	45.0	16
TCTACGG	35	1.2098826E-7	45.0	2
GTCGTTG	25	3.8872662E-5	45.0	1
CGTAATA	25	3.8872662E-5	45.0	24
>>END_MODULE