##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472197
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.986632697793507	33.0	31.0	34.0	30.0	34.0
2	31.995203273210123	33.0	31.0	34.0	30.0	34.0
3	32.026241166292884	33.0	31.0	34.0	30.0	34.0
4	35.70451315870283	37.0	35.0	37.0	33.0	37.0
5	34.75358377965129	37.0	35.0	37.0	33.0	37.0
6	35.22952496521579	37.0	35.0	37.0	33.0	37.0
7	35.766792249844876	37.0	35.0	37.0	33.0	37.0
8	35.7494266164334	37.0	35.0	37.0	35.0	37.0
9	37.53720586958409	39.0	37.0	39.0	35.0	39.0
10	37.24951450348054	39.0	37.0	39.0	34.0	39.0
11	37.18227138249502	39.0	37.0	39.0	34.0	39.0
12	37.098101004453646	39.0	37.0	39.0	34.0	39.0
13	36.99133412537564	39.0	37.0	39.0	33.0	39.0
14	38.157203455337495	40.0	37.0	41.0	33.0	41.0
15	38.21678452002025	40.0	37.0	41.0	33.0	41.0
16	38.295452957134415	40.0	37.0	41.0	34.0	41.0
17	38.185248953297034	40.0	37.0	41.0	34.0	41.0
18	38.17956064947469	40.0	37.0	41.0	34.0	41.0
19	38.13723085915412	40.0	37.0	41.0	34.0	41.0
20	38.00963792654337	40.0	36.0	41.0	34.0	41.0
21	38.00230624082745	40.0	36.0	41.0	34.0	41.0
22	38.03760294961637	40.0	37.0	41.0	34.0	41.0
23	38.059417997149495	40.0	37.0	41.0	34.0	41.0
24	38.04740394369299	40.0	36.0	41.0	34.0	41.0
25	37.922083367746936	40.0	36.0	41.0	34.0	41.0
26	37.94787133336298	40.0	36.0	41.0	34.0	41.0
27	37.881136474818774	40.0	36.0	41.0	34.0	41.0
28	37.59520496741826	40.0	36.0	41.0	33.0	41.0
29	37.4651046067637	40.0	36.0	41.0	33.0	41.0
30	37.48466635747368	40.0	36.0	41.0	33.0	41.0
31	37.4976291674873	40.0	36.0	41.0	33.0	41.0
32	37.330376940133036	40.0	36.0	41.0	32.0	41.0
33	37.31294142063588	40.0	36.0	41.0	32.0	41.0
34	37.23837932049547	40.0	36.0	41.0	32.0	41.0
35	37.14476161432622	40.0	35.0	41.0	32.0	41.0
36	37.08332115621234	40.0	35.0	41.0	31.0	41.0
37	37.06948582900781	40.0	35.0	41.0	32.0	41.0
38	37.04030944711635	40.0	35.0	41.0	31.0	41.0
39	36.954466038539	39.0	35.0	41.0	31.0	41.0
40	36.798279108084124	39.0	35.0	41.0	31.0	41.0
41	36.68671973773658	39.0	35.0	41.0	31.0	41.0
42	36.705807110168	39.0	35.0	41.0	31.0	41.0
43	36.58548444822818	39.0	35.0	41.0	31.0	41.0
44	36.552068310472116	39.0	35.0	41.0	30.0	41.0
45	36.48418350815444	39.0	35.0	41.0	30.0	41.0
46	36.358710453475986	39.0	35.0	41.0	30.0	41.0
47	36.25268902597856	39.0	35.0	41.0	30.0	41.0
48	36.186487842997735	39.0	35.0	41.0	30.0	41.0
49	36.23423486383861	39.0	35.0	41.0	30.0	41.0
50	36.08690228866342	39.0	35.0	40.0	30.0	41.0
51	34.76332759420327	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	9.0
11	7.0
12	6.0
13	4.0
14	5.0
15	16.0
16	23.0
17	41.0
18	100.0
19	223.0
20	500.0
21	892.0
22	1408.0
23	1908.0
24	2522.0
25	2998.0
26	3687.0
27	4365.0
28	5019.0
29	5818.0
30	7220.0
31	9216.0
32	12137.0
33	16722.0
34	28031.0
35	39790.0
36	33054.0
37	48290.0
38	87101.0
39	160958.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39481191113031	17.46961543593034	22.86418592240103	16.271386730538314
2	26.143537548946732	30.756866307918095	26.757476222847664	16.342119920287505
3	28.5675258419685	29.45423202603998	26.565183599218123	15.413058532773396
4	26.781830464827177	24.448270531155426	30.763219588434488	18.0066794155829
5	26.62321022793453	30.736747586282842	24.90231831206043	17.737723873722196
6	24.811254624658776	36.490913749981466	24.056696675328308	14.641134950031448
7	77.01849016406288	5.145733666245232	13.327276539241037	4.508499630450849
8	77.68770237845645	8.14829403829334	8.087514321353165	6.076489261897048
9	71.2539469755208	7.48839996865715	10.936960632956161	10.32069242286588
10	40.70504471650603	30.37926966922704	15.365620704917651	13.550064909349278
11	27.373109104886307	25.76361137406633	28.009072484577413	18.854207036469948
12	24.484060678064452	20.346380853753836	33.84328998278261	21.3262684853991
13	23.939160985775004	25.11494143334244	33.47035241647024	17.475545164412313
14	18.202995783539496	31.752425364837134	29.79519141375316	20.24938743787021
15	16.517258686522787	25.291350855681	38.30625776953263	19.885132688263585
16	18.941246979544555	26.24879022950167	30.109255247280263	24.70070754367351
17	19.693263616668467	25.453783060883488	28.869730218531668	25.983223103916377
18	22.55732247345917	23.35889469861096	30.124079568485186	23.95970325944468
19	22.795358716806756	26.25154331772544	26.990853393816565	23.96224457165124
20	22.4870128357444	25.12044760978998	30.785456070241867	21.607083484223747
21	22.99845191731417	28.684002651435737	28.869094890480035	19.44845054077006
22	20.380053240490728	24.7214615933604	29.16769907475058	25.730786091398294
23	19.215285145818374	27.042950294051	28.843893544431666	24.897871015698954
24	20.052859293896404	25.357001421017078	28.614751893807032	25.975387391279487
25	18.230314889759995	29.75918949082692	26.779924480672264	25.23057113874082
26	19.05370004468474	25.788812720114695	28.95338174533087	26.204105489869693
27	21.6691338572672	26.519228203482868	26.520498859586144	25.291139079663782
28	18.70786980857566	25.531504859200716	32.620283483376646	23.140341848846983
29	20.107921058371826	23.598836926113464	32.66115625469878	23.63208576081593
30	21.734572646586063	24.989358255135038	29.019667638718587	24.256401459560312
31	23.408873838673266	25.83730942805651	26.116006666709023	24.6378100665612
32	23.70451315870283	29.020302966770224	25.06729182946948	22.207892045057463
33	22.9264480714617	25.73459805970813	25.618121250240893	25.720832618589274
34	22.649656816964107	26.202834833766413	27.798990675501962	23.348517673767518
35	21.177813497332682	26.421599459547608	28.58277371520785	23.817813327911868
36	22.668504882496077	24.125947432957005	30.5654207883574	22.640126896189514
37	22.33432232733372	27.35320215926827	28.967147186449722	21.345328326948287
38	20.04925910160378	27.00059509060837	26.517745771362378	26.432400036425474
39	21.91415870918282	26.671918711893554	28.35596160077256	23.05796097815107
40	21.87709790617052	25.22252365008672	27.663454024485546	25.236924419257218
41	22.34088738386733	24.175926573019314	28.0200848374725	25.463101205640864
42	22.556898921424747	24.604349455841522	29.853429818486777	22.985321804246954
43	22.438939679837016	23.271219427484716	29.245420873067808	25.04442001961046
44	22.322674646386993	23.395955501623263	28.473285514308643	25.808084337681098
45	20.73731938152932	24.59376065498087	27.085305497493632	27.58361446599618
46	24.007564639334856	25.62849827508434	27.310211627774	23.053725457806806
47	19.91096936236359	23.606037310698714	31.398124088039523	25.08486923889817
48	20.146887845539045	24.766781661044014	29.178499651628453	25.907830841788492
49	20.32986232441121	21.791752171233618	33.09529708998575	24.783088414369423
50	20.86311433575393	21.94910175202299	30.905533072001727	26.282250840221348
51	20.565145479535023	22.355499929055036	28.217671861532367	28.861682729877575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	356.5
2	545.0
3	1138.5
4	1732.0
5	1232.0
6	732.0
7	707.5
8	683.0
9	691.5
10	700.0
11	749.5
12	799.0
13	788.5
14	778.0
15	703.0
16	628.0
17	679.0
18	730.0
19	847.5
20	965.0
21	1035.0
22	1105.0
23	1196.5
24	1288.0
25	1827.5
26	2860.5
27	3354.0
28	3787.5
29	4221.0
30	5459.0
31	6697.0
32	7708.5
33	8720.0
34	10151.0
35	11582.0
36	12654.5
37	13727.0
38	14906.5
39	16086.0
40	18958.0
41	21830.0
42	25204.0
43	28578.0
44	32991.0
45	37404.0
46	52556.5
47	67709.0
48	59474.0
49	51239.0
50	49471.0
51	47703.0
52	40782.0
53	33861.0
54	29547.0
55	25233.0
56	23295.0
57	21357.0
58	19181.0
59	17005.0
60	15690.5
61	14376.0
62	12543.5
63	10711.0
64	8689.5
65	6668.0
66	5573.5
67	4479.0
68	3460.0
69	2441.0
70	2036.0
71	1631.0
72	1365.5
73	1100.0
74	850.5
75	428.0
76	255.0
77	233.5
78	212.0
79	133.5
80	55.0
81	53.5
82	52.0
83	49.5
84	47.0
85	36.5
86	26.0
87	16.0
88	6.0
89	7.5
90	9.0
91	5.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.77925590523429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40713953635387	25.809763193854412
2	9.677278257976269	6.537825174846805
3	3.2935302462463776	3.33759003058757
4	1.5886279018114937	2.146506737339434
5	0.9955314164284527	1.681415523861853
6	0.6770024209752825	1.372118281595233
7	0.5615130693716843	1.3277245565107785
8	0.48354979524808905	1.3067161821287072
9	0.3831815897874277	1.1649230081643627
>10	5.724271140846828	43.16150660641359
>50	0.1688336569659356	3.5503233844586415
>100	0.031381720625638584	1.870566297687722
>500	0.0037658064750766304	1.1255623341747836
>1k	0.0031381720625638584	3.1373274479409394
>5k	0.0012552688250255435	2.4701312404351863
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	6242	1.3219058994445114	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	5409	1.1454964771059537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	4974	1.0533739096182315	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	4690	0.9932295207296955	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	2544	0.5387581877902655	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1571	0.33270012304186597	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	1019	0.2157997615402046	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	954	0.20203432042134956	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.19419860778446285	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	902	0.19102196752626552	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	872	0.18466868700987088	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	872	0.18466868700987088	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTA	792	0.16772660563281852	No Hit
TCGTAAGGTGGGAGGCATGGGGGTTAAGATTGTATAAACCATAGATCCTGT	493	0.10440557648608526	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	474	0.10038183215903532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.353280516394641E-4	0.0	0.0	0.21389377738528623	0.0
2	6.353280516394641E-4	0.0	0.0	1.3329182523395955	0.0
3	6.353280516394641E-4	0.0	0.0	1.7350809090273762	0.0
4	6.353280516394641E-4	0.0	0.0	3.237843527171922	0.0
5	6.353280516394641E-4	0.0	0.0	5.716046480600258	0.0
6	6.353280516394641E-4	0.0	0.0	6.895427120460316	0.0
7	6.353280516394641E-4	0.0	0.0	7.9115284510490325	0.0
8	6.353280516394641E-4	0.0	0.0	9.447539903896043	0.0
9	6.353280516394641E-4	0.0	0.0	10.024205998767464	0.0
10	6.353280516394641E-4	0.0	0.0	12.222864609474435	0.0
11	6.353280516394641E-4	0.0	0.0	13.484202567995984	0.0
12	6.353280516394641E-4	0.0	0.0	16.119331550179268	0.0
13	6.353280516394641E-4	0.0	0.0	16.975753763789267	0.0
14	6.353280516394641E-4	0.0	0.0	17.270545979749976	0.0
15	6.353280516394641E-4	0.0	0.0	17.752971746961542	0.0
16	6.353280516394641E-4	0.0	0.0	18.433831642301836	0.0
17	6.353280516394641E-4	0.0	0.0	19.27246467046593	0.0
18	6.353280516394641E-4	0.0	0.0	20.371158647767775	0.0
19	6.353280516394641E-4	0.0	0.0	21.164683384265466	0.0
20	6.353280516394641E-4	0.0	0.0	21.772904105701645	0.0
21	8.471040688526187E-4	0.0	0.0	22.396584476394388	0.0
22	8.471040688526187E-4	0.0	0.0	23.059866962305986	0.0
23	8.471040688526187E-4	0.0	0.0	23.66342861136348	0.0
24	8.471040688526187E-4	0.0	0.0	24.159408043676684	0.0
25	8.471040688526187E-4	0.0	0.0	24.579571661827586	0.0
26	8.471040688526187E-4	0.0	0.0	25.023242417889143	0.0
27	8.471040688526187E-4	0.0	0.0	25.5022797688253	0.0
28	0.0010588800860657733	0.0	0.0	25.94552697285243	0.0
29	0.0010588800860657733	0.0	0.0	26.41736393920334	0.0
30	0.0010588800860657733	0.0	0.0	26.97094644819853	0.0
31	0.0010588800860657733	0.0	0.0	27.49064479443961	0.0
32	0.0010588800860657733	0.0	0.0	27.91080841259051	0.0
33	0.0010588800860657733	0.0	0.0	28.380315842752072	0.0
34	0.0010588800860657733	0.0	0.0	28.833516519588223	0.0
35	0.0010588800860657733	0.0	0.0	29.34707336133012	0.0
36	0.0010588800860657733	0.0	0.0	29.87714873241465	0.0
37	0.0010588800860657733	0.0	0.0	30.317854624235224	0.0
38	0.0012706561032789282	0.0	0.0	30.75813696402137	0.0
39	0.0012706561032789282	0.0	0.0	31.198842855841946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAG	30	2.1627748E-6	45.000004	1
TCGTAAG	120	0.0	45.000004	1
CTAGCGG	65	0.0	45.000004	2
CGGGTTC	25	3.887303E-5	45.0	6
CGAAAGG	80	0.0	45.0	2
CCGCTCG	20	7.0290605E-4	45.0	19
ATCACGG	20	7.0290605E-4	45.0	2
CCGTAAT	20	7.0290605E-4	45.0	41
CGCTCGG	20	7.0290605E-4	45.0	20
GGGCGTA	20	7.0290605E-4	45.0	8
CGCTAGG	20	7.0290605E-4	45.0	2
AACGACG	25	3.887303E-5	45.0	1
GCGATAT	25	3.887303E-5	45.0	9
CCGCGTT	20	7.0290605E-4	45.0	45
CGGTAAT	20	7.0290605E-4	45.0	6
GCGCTAG	20	7.0290605E-4	45.0	1
CAATCGG	20	7.0290605E-4	45.0	2
GCCGTAA	20	7.0290605E-4	45.0	40
TCGAAGA	25	3.887303E-5	45.0	23
TAACTCG	20	7.0290605E-4	45.0	27
>>END_MODULE