##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935743.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852529
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14080224836926	33.0	31.0	34.0	30.0	34.0
2	32.187377790081044	33.0	31.0	34.0	30.0	34.0
3	32.19658920693607	34.0	31.0	34.0	30.0	34.0
4	35.85395335525243	37.0	35.0	37.0	35.0	37.0
5	34.946327925501656	37.0	35.0	37.0	33.0	37.0
6	35.3964674515471	37.0	35.0	37.0	33.0	37.0
7	35.92536558873657	37.0	35.0	37.0	35.0	37.0
8	35.90793861557788	37.0	35.0	37.0	35.0	37.0
9	37.733720495138584	39.0	38.0	39.0	35.0	39.0
10	37.44265473667171	39.0	37.0	39.0	35.0	39.0
11	37.38074599221844	39.0	37.0	39.0	35.0	39.0
12	37.33209896672137	39.0	37.0	39.0	35.0	39.0
13	37.273052294995246	39.0	37.0	39.0	34.0	39.0
14	38.409993091144116	40.0	38.0	41.0	34.0	41.0
15	38.501684986669076	40.0	38.0	41.0	34.0	41.0
16	38.51922808490972	40.0	38.0	41.0	34.0	41.0
17	38.480403599173755	40.0	38.0	41.0	34.0	41.0
18	38.41338652409478	40.0	38.0	41.0	34.0	41.0
19	38.3059250770355	40.0	37.0	41.0	34.0	41.0
20	38.255187800063105	40.0	37.0	41.0	34.0	41.0
21	38.18508578593807	40.0	37.0	41.0	34.0	41.0
22	38.23877428216518	40.0	37.0	41.0	34.0	41.0
23	38.20973831975217	40.0	37.0	41.0	34.0	41.0
24	38.18672209391117	40.0	37.0	41.0	34.0	41.0
25	38.02299393920911	40.0	36.0	41.0	34.0	41.0
26	38.039965795884946	40.0	36.0	41.0	34.0	41.0
27	38.03674009916378	40.0	36.0	41.0	34.0	41.0
28	37.87945395405904	40.0	36.0	41.0	34.0	41.0
29	37.82529509260095	40.0	36.0	41.0	33.0	41.0
30	37.6863696132331	40.0	36.0	41.0	33.0	41.0
31	37.60853531082227	40.0	36.0	41.0	33.0	41.0
32	37.497450526609654	40.0	36.0	41.0	33.0	41.0
33	37.316834969836805	40.0	36.0	41.0	33.0	41.0
34	37.17469200461216	40.0	35.0	41.0	32.0	41.0
35	37.06832494847683	40.0	35.0	41.0	32.0	41.0
36	36.95981368375738	40.0	35.0	41.0	31.0	41.0
37	36.90387071876734	40.0	35.0	41.0	31.0	41.0
38	36.81286618988914	40.0	35.0	41.0	31.0	41.0
39	36.7325428225902	39.0	35.0	41.0	31.0	41.0
40	36.61105370022603	39.0	35.0	41.0	31.0	41.0
41	36.4820692316625	39.0	35.0	41.0	30.0	41.0
42	36.49892027133388	39.0	35.0	41.0	30.0	41.0
43	36.39134739111514	39.0	35.0	41.0	30.0	41.0
44	36.290875735605475	39.0	35.0	41.0	30.0	41.0
45	36.23207421683016	39.0	35.0	41.0	30.0	41.0
46	36.122327803511666	39.0	35.0	41.0	30.0	41.0
47	36.009326368956366	39.0	35.0	41.0	30.0	41.0
48	35.95185266424954	38.0	35.0	41.0	30.0	41.0
49	35.91850599803643	38.0	35.0	40.0	30.0	41.0
50	35.79549903874238	38.0	35.0	40.0	29.0	41.0
51	34.60265750490599	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	13.0
10	19.0
11	25.0
12	28.0
13	24.0
14	37.0
15	37.0
16	61.0
17	119.0
18	213.0
19	462.0
20	827.0
21	1465.0
22	2066.0
23	2957.0
24	4168.0
25	6211.0
26	8016.0
27	9049.0
28	9667.0
29	10499.0
30	11897.0
31	15379.0
32	19998.0
33	27663.0
34	49457.0
35	62394.0
36	63023.0
37	92274.0
38	160032.0
39	294193.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64057762257941	17.25689096793188	20.8009346309627	20.30159677852601
2	30.686463451683167	26.353707615811313	25.712321809580672	17.24750712292485
3	30.42641364692579	25.098618346120777	27.996232386229675	16.478735620723754
4	27.343351369865427	25.255797749988563	29.302346313145943	18.098504567000067
5	24.869769826011783	29.74936922966843	26.597570287931553	18.78329065638823
6	25.702116878135524	33.73339792546646	25.83395990048432	14.730525295913688
7	77.68556846746563	5.648019011669985	11.931910820628975	4.734501700235418
8	78.75286353895292	5.816341731483621	10.442811916075582	4.98798281348787
9	72.23578318156919	6.759418154690339	12.891526270660588	8.113272393079884
10	37.97348829189388	27.01210164111719	19.336702915677943	15.677707151310983
11	28.454164022572837	25.399839770846505	27.299012702207197	18.84698350437346
12	25.55033318514678	21.640436864904302	32.19726249781532	20.61196745213359
13	24.63024718220729	22.692717784380356	33.94324415943622	18.733790873976133
14	21.007261923054816	26.51980167243578	31.39072101946092	21.082215385048485
15	19.409310416419853	24.15777058610323	35.96816061389114	20.464758383585778
16	22.968720125649686	24.519048618873963	30.930326123803415	21.58190513167294
17	23.164842486296656	24.444798945255823	29.352197989745804	23.03816057870172
18	24.013728565245287	23.698783267196777	30.733969167031265	21.55351900052667
19	23.743473829042767	26.50291075142312	27.988021521848523	21.76559389768559
20	24.336063641236837	25.474206742527233	28.919016244608684	21.270713371627238
21	24.139237492214342	27.026529302815504	29.2019391715707	19.63229403339945
22	22.92919067855756	24.018303189686215	29.313255032966623	23.7392510987896
23	21.836559225551273	25.477021896029346	29.87018623413397	22.816232644285414
24	21.94505993344508	24.542273635266366	30.422660108922983	23.09000632236557
25	22.668319787362073	26.970578126961076	28.079279414541908	22.28182267113494
26	21.66401377548447	25.24371604954201	28.82435670810025	24.267913466873267
27	22.289329747140567	24.896631082344413	29.044407873515155	23.76963129699987
28	21.41299592154636	25.256618836426682	30.048596587330167	23.281788654696793
29	22.085348416300207	24.762324800681267	29.67066222967195	23.481664553346572
30	22.760281468430986	25.349636200058885	30.543711709513694	21.346370621996435
31	24.927949665055383	25.1488219169084	27.755419463736718	22.1678089542995
32	25.04782828502022	25.351864863248053	27.239894478662897	22.360412373068833
33	23.66898955929945	25.280899535382375	27.478244141841508	23.571866763476667
34	24.209968223954846	24.20515900338874	29.619754870508803	21.96511790214761
35	23.958363879703796	25.840059399738895	28.1530598959097	22.048516824647606
36	25.07938146385636	26.78067256363127	27.727150630653036	20.41279534185934
37	23.71238984245697	27.656537196975115	27.446573664942775	21.184499295625134
38	24.016661016809984	26.597452989868962	27.841985433926585	21.543900559394462
39	23.760599346180598	25.65590144147589	27.22464573052647	23.35885348181704
40	25.273744353564513	23.872149803701692	29.851653140245084	21.002452702488714
41	23.007193890178517	25.486757635224137	28.21112243689071	23.294926037706635
42	22.93704964875095	25.810265691841565	28.659787526289428	22.592897133118054
43	22.65846675010469	25.242777665041306	28.927461705115014	23.17129387973899
44	23.19909352057232	24.350608600997738	28.852039050871	23.598258827558947
45	22.033619970698943	23.846109633807178	28.545304617203637	25.574965778290238
46	23.664180338733345	24.84032801230222	28.203732658947672	23.29175899001676
47	21.77028582018911	24.513770206057504	30.670393617108626	23.04555035664476
48	21.7897572985787	24.952347662073667	29.324984839225408	23.932910200122222
49	22.610491842506235	23.77209455631421	30.369406788508073	23.248006812671477
50	21.82318724641625	23.754147952738265	30.95906414913745	23.463600651708035
51	21.443610715881807	23.451401653198893	29.52720669912695	25.577780931792347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	957.5
2	1649.0
3	5061.0
4	8473.0
5	5629.5
6	2786.0
7	2780.0
8	2774.0
9	2797.0
10	2820.0
11	2798.5
12	2777.0
13	2715.5
14	2654.0
15	2619.0
16	2584.0
17	2528.0
18	2472.0
19	2515.5
20	2559.0
21	2799.0
22	3039.0
23	3292.5
24	3546.0
25	4151.0
26	6025.0
27	7294.0
28	8479.0
29	9664.0
30	11241.5
31	12819.0
32	14833.0
33	16847.0
34	20054.5
35	23262.0
36	25227.5
37	27193.0
38	28916.0
39	30639.0
40	33447.0
41	36255.0
42	39471.0
43	42687.0
44	47048.0
45	51409.0
46	66520.5
47	81632.0
48	80287.5
49	78943.0
50	77369.5
51	75796.0
52	66921.0
53	58046.0
54	52710.5
55	47375.0
56	44493.5
57	41612.0
58	39287.5
59	36963.0
60	34984.0
61	33005.0
62	30292.5
63	27580.0
64	24610.0
65	21640.0
66	19184.5
67	16729.0
68	14095.0
69	11461.0
70	9895.0
71	8329.0
72	6770.0
73	5211.0
74	4233.0
75	2626.5
76	1998.0
77	1491.0
78	984.0
79	714.5
80	445.0
81	285.5
82	126.0
83	110.5
84	95.0
85	72.0
86	49.0
87	29.0
88	9.0
89	9.0
90	9.0
91	7.0
92	5.0
93	3.5
94	2.0
95	3.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	852529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.74393994852903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96926440666924	24.960144161755128
2	8.490806291992032	5.730265152631432
3	3.3331618240413885	3.374220372875463
4	1.9925427232096147	2.68944967987455
5	1.4082237298634872	2.375950848730355
6	1.1266844995255698	2.281126445575759
7	0.9179854483613261	2.1683512090189665
8	0.7987889414508595	2.1563428857493507
9	0.7186466346468283	2.1824971995361557
>10	7.131461418955393	44.79805766733398
>50	0.07611997574557518	1.6959748575991602
>100	0.029679797796056086	1.8930958146240735
>500	0.0027933927337458913	0.612337023003686
>1k	0.003142566825464128	1.8473796774436775
>5k	6.983481834364728E-4	1.2348070042483412
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	5464	0.6409166139802869	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5016	0.5883670819409076	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	2963	0.3475541594479484	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	2921	0.3426276408192566	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1870	0.21934737703937346	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1819	0.2133651758473905	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT	1714	0.20104887927566098	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	1196	0.14028848285512868	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTT	1134	0.13301600297467886	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	1040	0.12198998509141624	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTT	1022	0.11987861996483405	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	998	0.11706346646272443	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1729806258790024E-4	0.0	0.0	0.12421864828058635	0.0
2	1.1729806258790024E-4	0.0	0.0	0.6544058911778954	0.0
3	1.1729806258790024E-4	0.0	0.0	1.0384397480906808	0.0
4	1.1729806258790024E-4	0.0	0.0	1.5739054038044453	0.0
5	1.1729806258790024E-4	0.0	0.0	3.04505770478189	0.0
6	1.1729806258790024E-4	0.0	0.0	4.411110941680576	0.0
7	1.1729806258790024E-4	0.0	0.0	5.28685827696184	0.0
8	1.1729806258790024E-4	0.0	0.0	6.594966270942103	0.0
9	1.1729806258790024E-4	0.0	0.0	7.26602848700748	0.0
10	1.1729806258790024E-4	0.0	0.0	8.623049773086898	0.0
11	1.1729806258790024E-4	0.0	0.0	9.509588530126248	0.0
12	1.1729806258790024E-4	0.0	0.0	10.715178017404687	0.0
13	1.1729806258790024E-4	0.0	0.0	11.221201859408888	0.0
14	1.1729806258790024E-4	0.0	0.0	11.477028933913099	0.0
15	1.1729806258790024E-4	0.0	0.0	11.771212474883553	0.0
16	1.1729806258790024E-4	0.0	0.0	12.200171489767504	0.0
17	1.1729806258790024E-4	0.0	0.0	12.664906413740765	0.0
18	1.1729806258790024E-4	0.0	0.0	13.297025673026958	0.0
19	1.1729806258790024E-4	0.0	0.0	13.678830866750573	0.0
20	1.1729806258790024E-4	0.0	0.0	14.075532914422853	0.0
21	1.1729806258790024E-4	0.0	0.0	14.46167813646222	0.0
22	1.1729806258790024E-4	0.0	0.0	14.895680968037452	0.0
23	1.1729806258790024E-4	0.0	0.0	15.311033407661206	0.0
24	1.1729806258790024E-4	0.0	0.0	15.652605365917172	0.0
25	1.1729806258790024E-4	0.0	0.0	15.965204702713926	0.0
26	1.1729806258790024E-4	0.0	0.0	16.273698607320103	0.0
27	1.1729806258790024E-4	0.0	0.0	16.6401377548447	0.0
28	3.518941877637007E-4	0.0	0.0	16.975962108033862	0.0
29	3.518941877637007E-4	0.0	0.0	17.32867738223568	0.0
30	3.518941877637007E-4	0.0	0.0	17.83399743586435	0.0
31	3.518941877637007E-4	0.0	0.0	18.211345303209626	0.0
32	3.518941877637007E-4	0.0	0.0	18.592915900808066	0.0
33	3.518941877637007E-4	0.0	0.0	18.95865125995714	0.0
34	3.518941877637007E-4	0.0	0.0	19.316644946975412	0.0
35	3.518941877637007E-4	0.0	0.0	19.692937131757393	0.0
36	3.518941877637007E-4	0.0	0.0	20.030286359760197	0.0
37	3.518941877637007E-4	0.0	0.0	20.3831189320246	0.0
38	3.518941877637007E-4	0.0	0.0	20.76081869355764	0.0
39	3.518941877637007E-4	0.0	0.0	21.272590140628648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.032261E-4	45.0	1
TAACGCC	25	3.8899547E-5	45.0	12
TATAGCG	20	7.032261E-4	45.0	1
CGTTTTT	2610	0.0	42.327583	1
CGACGGT	80	0.0	42.1875	28
ATTCGAC	45	1.9272193E-8	40.0	42
TTCGACT	45	1.9272193E-8	40.0	43
CTCACGA	70	0.0	38.571426	24
CGGTCTA	70	0.0	38.571426	31
GATTACG	35	6.2463914E-6	38.571426	1
ATCACGG	55	2.746674E-9	36.81818	2
TCACGAC	75	1.8189894E-12	36.000004	25
TCGTTAG	25	0.0021068037	36.0	1
CTATCGG	25	0.0021068037	36.0	2
GTCGTAA	25	0.0021068037	36.0	9
TTCGCAC	25	0.0021068037	36.0	37
TCGAACG	25	0.0021068037	36.0	1
ATGGGCG	265	0.0	35.660378	5
TCGATGG	150	0.0	34.500004	2
CGAATAT	85	0.0	34.411766	14
>>END_MODULE