##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935742.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520679
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95215286193605	33.0	31.0	34.0	30.0	34.0
2	31.99668125658995	33.0	31.0	34.0	30.0	34.0
3	32.04134024994286	33.0	31.0	34.0	30.0	34.0
4	35.712796175762804	37.0	35.0	37.0	33.0	37.0
5	34.802817090760335	37.0	35.0	37.0	33.0	37.0
6	35.249793058679145	37.0	35.0	37.0	33.0	37.0
7	35.784980765500435	37.0	35.0	37.0	35.0	37.0
8	35.71931650786761	37.0	35.0	37.0	35.0	37.0
9	37.54250315453475	39.0	37.0	39.0	35.0	39.0
10	37.217998037178376	39.0	37.0	39.0	34.0	39.0
11	37.170183548789176	39.0	37.0	39.0	34.0	39.0
12	37.1370594934691	39.0	37.0	39.0	34.0	39.0
13	37.01919992932306	39.0	37.0	39.0	33.0	39.0
14	38.15934193620254	40.0	37.0	41.0	33.0	41.0
15	38.22216375156286	40.0	37.0	41.0	33.0	41.0
16	38.28989646211966	40.0	37.0	41.0	34.0	41.0
17	38.22257091221271	40.0	37.0	41.0	34.0	41.0
18	38.200059921756015	40.0	37.0	41.0	34.0	41.0
19	38.11901766731518	40.0	37.0	41.0	34.0	41.0
20	38.03429752304203	40.0	37.0	41.0	33.0	41.0
21	38.042625110672795	40.0	37.0	41.0	34.0	41.0
22	38.08699025695294	40.0	37.0	41.0	34.0	41.0
23	38.03921994165311	40.0	37.0	41.0	34.0	41.0
24	38.00676232381179	40.0	37.0	41.0	34.0	41.0
25	37.8886242771458	40.0	36.0	41.0	33.0	41.0
26	37.88942131332357	40.0	36.0	41.0	33.0	41.0
27	37.85831961726899	40.0	36.0	41.0	33.0	41.0
28	37.73649599849428	40.0	36.0	41.0	33.0	41.0
29	37.64903712268019	40.0	36.0	41.0	33.0	41.0
30	37.59511138340513	40.0	36.0	41.0	33.0	41.0
31	37.54351913559026	40.0	36.0	41.0	33.0	41.0
32	37.402758705459604	40.0	36.0	41.0	32.0	41.0
33	37.28395230074576	40.0	36.0	41.0	32.0	41.0
34	37.2109956422287	40.0	36.0	41.0	32.0	41.0
35	37.12939450217888	40.0	35.0	41.0	32.0	41.0
36	37.05251604155343	40.0	35.0	41.0	31.0	41.0
37	36.992075731880874	40.0	35.0	41.0	31.0	41.0
38	36.87630958805713	39.0	35.0	41.0	31.0	41.0
39	36.75465689993259	39.0	35.0	41.0	31.0	41.0
40	36.665899719404855	39.0	35.0	41.0	31.0	41.0
41	36.60459131249772	39.0	35.0	41.0	31.0	41.0
42	36.65551136112653	39.0	35.0	41.0	31.0	41.0
43	36.53163657454977	39.0	35.0	41.0	31.0	41.0
44	36.43977575435153	39.0	35.0	41.0	30.0	41.0
45	36.3873903114971	39.0	35.0	41.0	30.0	41.0
46	36.2417266684464	39.0	35.0	41.0	30.0	41.0
47	36.09716351149172	39.0	35.0	41.0	30.0	41.0
48	36.04185880360068	38.0	35.0	40.0	29.0	41.0
49	35.991597510174216	38.0	35.0	40.0	29.0	41.0
50	35.780021856076395	38.0	35.0	40.0	29.0	41.0
51	34.56234839507643	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	12.0
11	14.0
12	10.0
13	18.0
14	11.0
15	16.0
16	28.0
17	61.0
18	164.0
19	285.0
20	625.0
21	1122.0
22	1557.0
23	2083.0
24	2503.0
25	3232.0
26	4118.0
27	4726.0
28	5303.0
29	6430.0
30	8245.0
31	10516.0
32	13792.0
33	18504.0
34	31074.0
35	41412.0
36	38382.0
37	57166.0
38	100630.0
39	168491.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99908580910695	18.079661365255753	21.31197916566637	16.60927365997092
2	26.882781905934365	30.56374464881434	25.017141079244603	17.536332366006697
3	28.83004691950319	29.00597104934134	26.29604804495668	15.86793398619879
4	26.904676393708982	24.68507468132957	30.581413884562274	17.82883504039917
5	27.214464190028792	30.08302620232427	24.079327186231826	18.623182421415112
6	24.43155955972874	36.74452013620676	24.4033271939141	14.420593110150401
7	75.03240960361374	6.52359707228446	13.673491729069157	4.7705015950326395
8	75.76606700097372	9.320137743216069	9.216427011652092	5.697368244158109
9	69.93925239927096	7.552061826960566	11.822639284472775	10.686046489295709
10	38.06952076039172	26.509231215393747	19.161133827175668	16.260114197038867
11	29.88194261723634	25.076486664528435	25.805150582220527	19.236420136014704
12	26.61908776808744	21.39264306799391	30.414132315687787	21.574136848230868
13	24.14520270646598	24.8072228762827	32.294561524470936	18.753012892780387
14	20.01156182600028	30.073039242988482	28.706746383088237	21.208652547923002
15	17.923903210999484	25.853164809796436	36.46142825041917	19.761503728784913
16	20.45098803677506	25.229940135861057	30.775199307058664	23.543872520305218
17	20.36552271168993	25.867569078069213	28.78088035046545	24.986027859775408
18	21.454869506932294	25.411625973008324	30.006011381292502	23.12749313876688
19	22.097107814987737	28.044918270181817	26.943856003410932	22.914117911419513
20	24.141361568259907	25.786713118831372	29.126198675191432	20.94572663771729
21	22.550554180214682	29.004626650969218	28.120780749751766	20.324038419064337
22	20.33095246783527	25.289669834965494	28.592856635278164	25.786521061921068
23	20.346124963749258	28.049335579118807	28.429992375340664	23.174547081791278
24	21.390338385070265	25.482302916000073	28.787026171595166	24.3403325273345
25	19.80855767180931	29.73040971500675	27.070037393480433	23.390995219703502
26	20.20457902085546	26.07825550867233	28.679858415645725	25.037307054826485
27	22.43935322914886	27.070229450390737	26.878364596997383	23.612052723463016
28	20.487670906643057	26.95307473510551	29.865425722950224	22.693828635301212
29	22.74337931815956	25.126229404297078	28.399071212781774	23.73132006476159
30	21.809022449532243	26.953458848926115	28.22852467643212	23.00899402510952
31	24.575794299366787	24.944735624060122	25.85950268783646	24.61996738873663
32	24.601721982257782	26.976697735072857	27.647744579673848	20.77383570299551
33	23.04164369986114	26.721262044368988	25.80611086677204	24.43098338899783
34	24.04494899928747	24.47304385235433	29.23106174821723	22.25094540014097
35	21.054238792038856	23.829653202837065	29.874260340824193	25.24184766429989
36	26.863960328724605	25.737930663614243	27.212351564015446	20.185757443645702
37	21.799611660927365	24.68296205531623	31.77581580974074	21.74161047401566
38	22.360801952834663	27.052752271553103	28.07776000184375	22.508685773768484
39	23.42556546355816	24.899986363959368	30.36362134827792	21.310826824204547
40	21.675927010691808	24.975272672798404	31.838042248679127	21.51075806783066
41	21.062497239181916	25.47404446885701	29.75038363367833	23.713074658282743
42	22.74971719619958	23.862686991409294	30.60023546177203	22.787360350619096
43	24.933596323262506	23.653921129909214	27.898378847620126	23.51410369920815
44	23.000351464145854	24.35934616145456	28.38639545670173	24.25390691769785
45	20.946494865358503	24.137520430053833	28.780688293555144	26.135296411032517
46	24.469202714148256	25.890039736574742	27.652738059341743	21.988019489935258
47	20.663210922660603	23.905323625496706	33.25350167761711	22.177963774225578
48	22.00127141674621	23.5586609023986	29.90556561720369	24.5345020636515
49	21.584507921387267	22.832493724540456	32.654476174379994	22.928522179692287
50	21.580858840091498	22.573216895630512	31.596434655517125	24.249489608760868
51	20.630945361729587	22.792545887197292	28.715773057872507	27.86073569320061
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	164.0
1	535.0
2	906.0
3	1496.5
4	2087.0
5	1458.5
6	830.0
7	856.0
8	882.0
9	876.0
10	870.0
11	902.0
12	934.0
13	1024.5
14	1115.0
15	1083.0
16	1051.0
17	1054.5
18	1058.0
19	1097.5
20	1137.0
21	1454.0
22	1771.0
23	1888.0
24	2005.0
25	2615.0
26	3733.0
27	4241.0
28	4847.5
29	5454.0
30	6690.0
31	7926.0
32	9095.5
33	10265.0
34	11334.5
35	12404.0
36	14319.5
37	16235.0
38	17860.5
39	19486.0
40	22014.5
41	24543.0
42	27289.0
43	30035.0
44	34149.0
45	38263.0
46	53281.5
47	68300.0
48	62288.0
49	56276.0
50	53225.0
51	50174.0
52	42906.0
53	35638.0
54	31716.0
55	27794.0
56	24714.0
57	21634.0
58	20383.0
59	19132.0
60	17981.5
61	16831.0
62	14845.5
63	12860.0
64	10810.5
65	8761.0
66	7466.0
67	6171.0
68	5044.5
69	3918.0
70	3268.5
71	2619.0
72	2094.0
73	1569.0
74	1330.5
75	771.5
76	451.0
77	397.0
78	343.0
79	214.0
80	85.0
81	88.5
82	92.0
83	56.5
84	21.0
85	22.0
86	23.0
87	12.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.057216354066064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86393131137825	25.496571057875496
2	9.737679009880939	6.632764816519258
3	3.5793361434783946	3.657066763271164
4	1.894320747984583	2.5806116623242876
5	1.237615528428414	2.1074869907419145
6	0.9402880971344152	1.921415709555592
7	0.7234742676306383	1.72476637615073
8	0.5897234900354158	1.606747239136886
9	0.48713839083887667	1.4931519814054095
>10	5.694362371460581	40.64640525714916
>50	0.20329452645573381	4.254947720946324
>100	0.041453911819185875	2.302789078374065
>500	0.0028393090287113613	0.8159458404014598
>1k	0.0028393090287113613	1.5309379645930208
>5k	0.001703585417226817	3.228391541555242
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	6580	1.2637344697980906	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	5065	0.9727682506880438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	5048	0.9695032832128816	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	2492	0.4786058204767236	TruSeq Adapter, Index 22 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1677	0.3220794385792398	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1505	0.2890456500070101	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1178	0.22624304033771286	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	1064	0.20434855256309548	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTC	995	0.19109662575214287	No Hit
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	872	0.16747362578479255	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGTATTGT	867	0.16651334123327424	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	761	0.14615530874108615	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTA	724	0.1390492030598507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.841138206073224E-4	0.0	0.0	0.1786129265824049	0.0
2	3.841138206073224E-4	0.0	0.0	1.1511891203601452	0.0
3	3.841138206073224E-4	0.0	0.0	1.5306935751201796	0.0
4	3.841138206073224E-4	0.0	0.0	2.297000647231788	0.0
5	3.841138206073224E-4	0.0	0.0	4.631068278152182	0.0
6	3.841138206073224E-4	0.0	0.0	5.855239024427719	0.0
7	3.841138206073224E-4	0.0	0.0	6.886776689668682	0.0
8	3.841138206073224E-4	0.0	0.0	8.493716858179416	0.0
9	3.841138206073224E-4	0.0	0.0	9.106378402048096	0.0
10	3.841138206073224E-4	0.0	0.0	11.226110521069604	0.0
11	3.841138206073224E-4	0.0	0.0	12.462572909604575	0.0
12	3.841138206073224E-4	0.0	0.0	14.653366085438437	0.0
13	3.841138206073224E-4	0.0	0.0	15.237987320402782	0.0
14	3.841138206073224E-4	0.0	0.0	15.503025856621834	0.0
15	3.841138206073224E-4	0.0	0.0	15.989506010421008	0.0
16	3.841138206073224E-4	0.0	0.0	16.676109464756596	0.0
17	3.841138206073224E-4	0.0	0.0	17.511557024577524	0.0
18	3.841138206073224E-4	0.0	0.0	18.546551714203954	0.0
19	3.841138206073224E-4	0.0	0.0	19.264844558739647	0.0
20	3.841138206073224E-4	0.0	0.0	19.84850550915247	0.0
21	3.841138206073224E-4	0.0	0.0	20.483445654616375	0.0
22	3.841138206073224E-4	0.0	0.0	21.20404318207571	0.0
23	3.841138206073224E-4	0.0	0.0	21.840135669001437	0.0
24	3.841138206073224E-4	0.0	0.0	22.336602782136403	0.0
25	5.761707309109835E-4	0.0	0.0	22.797731423775492	0.0
26	5.761707309109835E-4	0.0	0.0	23.205468244350165	0.0
27	5.761707309109835E-4	0.0	0.0	23.695789536355413	0.0
28	5.761707309109835E-4	0.0	0.0	24.143474194273246	0.0
29	5.761707309109835E-4	0.0	0.0	24.644166559434893	0.0
30	5.761707309109835E-4	0.0	0.0	25.293703030081875	0.0
31	5.761707309109835E-4	0.0	0.0	25.793627167602303	0.0
32	5.761707309109835E-4	0.0	0.0	26.260901630371112	0.0
33	5.761707309109835E-4	0.0	0.0	26.72875226387083	0.0
34	5.761707309109835E-4	0.0	0.0	27.180662173815346	0.0
35	5.761707309109835E-4	0.0	0.0	27.673096091833933	0.0
36	5.761707309109835E-4	0.0	0.0	28.144979920450027	0.0
37	7.682276412146448E-4	0.0	0.0	28.59420103365029	0.0
38	7.682276412146448E-4	0.0	0.0	29.06358812243244	0.0
39	7.682276412146448E-4	0.0	0.0	29.5285579022776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	45	3.8380676E-10	45.000004	1
TAGTGCG	35	1.21021E-7	45.000004	1
CTATGCG	25	3.8878563E-5	45.0	1
AGTCCGA	20	7.0297276E-4	45.0	11
CGTGCTT	20	7.0297276E-4	45.0	42
GGCACGC	80	0.0	45.0	8
CCGTGTA	20	7.0297276E-4	45.0	26
CGTGTAT	20	7.0297276E-4	45.0	27
CAACCGG	30	2.1632059E-6	44.999996	2
ACTACCG	30	2.1632059E-6	44.999996	13
CACGGGC	80	0.0	42.1875	4
ATGACGG	110	0.0	40.909092	2
TCGAATG	55	6.002665E-11	40.909092	44
GGGCACG	100	0.0	40.5	7
CGGTCTA	45	1.9248546E-8	40.000004	31
CGATGAA	360	0.0	39.375004	19
TATGCGG	110	0.0	38.863636	2
CGGGTGA	105	0.0	38.57143	6
CGCGAGG	95	0.0	37.894737	2
TAACGGG	150	0.0	37.500004	3
>>END_MODULE