##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935741.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1196389
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.076261985023265	33.0	31.0	34.0	30.0	34.0
2	32.15062241461598	33.0	31.0	34.0	30.0	34.0
3	32.12764828162078	33.0	31.0	34.0	30.0	34.0
4	35.80840763330321	37.0	35.0	37.0	35.0	37.0
5	34.91917929703466	37.0	35.0	37.0	33.0	37.0
6	35.366322324929435	37.0	35.0	37.0	33.0	37.0
7	35.916811338118286	37.0	35.0	37.0	35.0	37.0
8	35.90468401163835	37.0	35.0	37.0	35.0	37.0
9	37.74907659632444	39.0	38.0	39.0	35.0	39.0
10	37.46959224800629	39.0	37.0	39.0	35.0	39.0
11	37.3638632585221	39.0	37.0	39.0	35.0	39.0
12	37.23830459825358	39.0	37.0	39.0	34.0	39.0
13	37.1806778564497	39.0	37.0	39.0	34.0	39.0
14	38.2267874412085	40.0	37.0	41.0	33.0	41.0
15	38.24484845648029	40.0	37.0	41.0	34.0	41.0
16	38.254469909034604	40.0	37.0	41.0	34.0	41.0
17	38.21176139198872	40.0	37.0	41.0	34.0	41.0
18	38.1385778371416	40.0	37.0	41.0	34.0	41.0
19	38.045515296446226	40.0	37.0	41.0	34.0	41.0
20	37.98436294549683	40.0	36.0	41.0	34.0	41.0
21	37.921207901443424	40.0	36.0	41.0	34.0	41.0
22	37.924658284220264	40.0	36.0	41.0	34.0	41.0
23	37.90296049194702	40.0	36.0	41.0	34.0	41.0
24	37.899139828266556	40.0	35.0	41.0	34.0	41.0
25	37.75517912652156	40.0	35.0	41.0	33.0	41.0
26	37.76463006597353	40.0	35.0	41.0	33.0	41.0
27	37.781864427038364	40.0	35.0	41.0	34.0	41.0
28	37.67223871165649	40.0	35.0	41.0	33.0	41.0
29	37.59282223423987	40.0	35.0	41.0	33.0	41.0
30	37.425278901761885	40.0	35.0	41.0	33.0	41.0
31	37.27254596958013	40.0	35.0	41.0	33.0	41.0
32	37.091577237838194	39.0	35.0	41.0	32.0	41.0
33	36.818857411761556	39.0	35.0	41.0	31.0	41.0
34	36.61464456794571	39.0	35.0	41.0	31.0	41.0
35	36.46047230457652	39.0	35.0	41.0	30.0	41.0
36	36.370518284604756	39.0	35.0	41.0	30.0	41.0
37	36.30032205244281	39.0	35.0	41.0	30.0	41.0
38	36.210840286896655	39.0	35.0	41.0	30.0	41.0
39	36.129211318392265	39.0	35.0	41.0	30.0	41.0
40	35.99888079880373	39.0	35.0	41.0	29.0	41.0
41	35.86281301483046	39.0	35.0	41.0	28.0	41.0
42	35.857624067088544	39.0	35.0	41.0	28.0	41.0
43	35.73412410177626	38.0	35.0	41.0	28.0	41.0
44	35.593672292205966	38.0	35.0	40.0	28.0	41.0
45	35.528018061015274	38.0	35.0	40.0	28.0	41.0
46	35.39506046946269	38.0	35.0	40.0	27.0	41.0
47	35.247299164402214	38.0	35.0	40.0	26.0	41.0
48	35.16929527101971	38.0	34.0	40.0	26.0	41.0
49	35.14120407325711	37.0	34.0	40.0	27.0	41.0
50	35.01894701472514	37.0	34.0	40.0	26.0	41.0
51	33.90197419066875	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	25.0
10	25.0
11	21.0
12	24.0
13	31.0
14	36.0
15	50.0
16	84.0
17	213.0
18	350.0
19	587.0
20	1125.0
21	1850.0
22	2760.0
23	4165.0
24	6612.0
25	10430.0
26	15021.0
27	18048.0
28	18169.0
29	18298.0
30	19889.0
31	23840.0
32	30348.0
33	42060.0
34	76244.0
35	97330.0
36	92698.0
37	136381.0
38	227764.0
39	351675.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.38966924637388	16.945826148518584	20.587283901807858	26.07722070329968
2	31.779880958450807	25.142825619426457	26.11140690862253	16.96588651350021
3	29.897466459487674	23.89222903253039	30.74760801043808	15.46269649754386
4	26.542203246602902	24.27905973725937	31.318492563873455	17.860244452264272
5	23.754648362698084	29.13684428726777	28.787543182025242	18.3209641680089
6	24.95066403987332	32.210008617598454	27.877220536129972	14.962106806398253
7	76.0512676061047	5.188195478226564	13.75764905895992	5.0028878567088135
8	77.48182238385675	4.6518314695303955	12.720277434847697	5.146068711765153
9	71.50341569506239	5.722302695862299	15.181099124114313	7.593182484960995
10	42.78073435981106	22.578191541379937	19.812285134684455	14.828788964124545
11	31.22170130283712	22.432503140700895	27.473589275728884	18.872206280733106
12	27.421097987360298	19.789382884663766	31.82685564644944	20.962663481526494
13	24.921075001525423	20.18624377188356	34.88447319391937	20.008208032671646
14	22.2441864644359	22.543002317808003	35.17676942867244	20.03604178908365
15	21.286805545687898	21.06029059110373	37.95588224231417	19.697021620894205
16	24.20508714138963	21.77519184813635	34.415812917036185	19.603908093437834
17	25.149512407753665	21.45690072376125	32.61915647836949	20.77443039011559
18	24.75265152053387	23.47221514072764	31.63904047930899	20.1360928594295
19	22.993441096499552	28.152883384919118	28.725523220290388	20.128152298290942
20	22.988593174962325	28.394861537509957	28.61260008241467	20.00394520511305
21	25.143577883113267	27.184218510868956	29.031276616551978	18.6409269894658
22	23.15985854099294	23.261664893274677	31.22445960302209	22.35401696271029
23	20.78404264833595	24.176417536436727	33.23927251086394	21.80026730436338
24	21.34681947092459	22.40609032680842	34.11239989668912	22.134690305577866
25	22.837137419351063	22.13502464499423	32.765597142735345	22.26224079291936
26	20.536380725666987	22.014495285396304	32.590904797687045	24.85821919124967
27	21.309540626000405	23.073766141280135	30.60100017636404	25.015693056355413
28	20.75654323134031	24.014513674064204	32.02628910830842	23.202653986287068
29	22.307878123252554	22.202728376807208	30.047334102871226	25.442059397069013
30	23.03740672975094	23.55513131598502	26.595697553220564	26.811764401043476
31	22.70958693200957	26.987877688611313	25.160712778201738	25.141822601177378
32	23.561818104312227	29.2193425382547	24.486350175402816	22.73248918203026
33	22.521604595160937	27.36392594716267	25.65871133887055	24.45575811880584
34	23.585639787727906	25.44038769998721	26.169665551923327	24.804306960361554
35	22.779798209445257	27.512038308610325	23.756570814342158	25.95159266760226
36	21.61412383430473	29.45480107222651	23.395400659818836	25.535674433649923
37	20.897132955919854	29.288634382295392	24.67483402137599	25.13939864040876
38	20.762143416564342	25.68253302228623	25.79729502695194	27.758028534197486
39	20.289888990955284	24.390227593199203	26.362997319433727	28.95688609641179
40	21.2462668914542	22.62391245656722	28.822732405597172	27.3070882463814
41	19.756366867298176	23.070506331970623	31.056955555425535	26.116171245305665
42	22.502714418136577	22.973213561809747	30.65817221656167	23.865899803492006
43	22.981906386635117	21.92414005812491	31.16310832011996	23.930845235120017
44	22.324845848632844	21.353171919835436	32.77596166464252	23.546020566889197
45	21.870144242382704	20.635512362617845	33.1290240883191	24.36531930668035
46	25.658794923724642	21.02543570694816	30.22787738770584	23.08789198162136
47	25.60530061710698	20.916524642068758	30.520006452750735	22.958168288073526
48	23.48140947467755	20.760889643752993	30.09338935747487	25.66431152409459
49	22.604186431001956	20.267822589475497	31.22211922710757	25.905871752414978
50	22.107107303728135	19.649629008625123	33.670068848844316	24.57319483880243
51	21.124233004482658	19.834100781601972	32.7153626454272	26.326303568488175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	353.0
1	1077.0
2	1801.0
3	11747.0
4	21693.0
5	14257.5
6	6822.0
7	6657.5
8	6493.0
9	6462.5
10	6432.0
11	6411.0
12	6390.0
13	6250.0
14	6110.0
15	5730.5
16	5351.0
17	4967.0
18	4583.0
19	4129.5
20	3676.0
21	3613.5
22	3551.0
23	3501.5
24	3452.0
25	3837.0
26	4676.5
27	5131.0
28	6009.5
29	6888.0
30	8310.5
31	9733.0
32	11231.0
33	12729.0
34	15204.0
35	17679.0
36	19745.5
37	21812.0
38	26773.5
39	31735.0
40	38768.5
41	45802.0
42	54268.5
43	62735.0
44	70080.0
45	77425.0
46	98002.5
47	118580.0
48	118038.5
49	117497.0
50	114829.0
51	112161.0
52	99511.0
53	86861.0
54	76708.5
55	66556.0
56	62650.5
57	58745.0
58	56832.5
59	54920.0
60	51842.5
61	48765.0
62	45987.5
63	43210.0
64	39928.5
65	36647.0
66	31930.5
67	27214.0
68	23722.0
69	20230.0
70	16796.0
71	13362.0
72	10824.5
73	8287.0
74	6778.5
75	4104.0
76	2938.0
77	2179.5
78	1421.0
79	1038.0
80	655.0
81	446.5
82	238.0
83	179.5
84	121.0
85	83.5
86	46.0
87	38.0
88	30.0
89	22.0
90	14.0
91	15.0
92	16.0
93	10.0
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1196389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.153634432731636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81223162575768	24.184950723120455
2	8.502628781609806	5.807913502486474
3	3.896134989669381	3.992015104132281
4	2.526840819266414	3.4520319044371686
5	1.9324056040010689	3.299933728740724
6	1.6043416631462353	3.287645920097832
7	1.385279829082961	3.311863860867549
8	1.188856355526142	3.248301228773559
9	1.0236640760338511	3.1465663771302137
>10	7.033412930832093	38.58520685144568
>50	0.0601550766597736	1.3482135043554444
>100	0.027835064648233074	1.6719035261741313
>500	0.0019882188052574162	0.38833113685028425
>1k	0.0037279102598576554	2.5737654058085946
>5k	2.4852735065717703E-4	0.6066453847145316
>10k+	2.4852735065717703E-4	1.094711840865162
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12897	1.0779938632000128	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	7147	0.5973809521819408	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT	4051	0.3386022439189929	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT	2704	0.22601344545962895	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	2576	0.21531458413609622	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	2466	0.20612025018618527	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	2415	0.2018574226275902	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	2409	0.20135591350304957	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	2207	0.1844717729768495	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	2140	0.17887158775281284	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	2028	0.1695100840947217	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	1551	0.12964010869374426	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	1421	0.11877407766203132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5075456227029836E-4	0.0	0.0	0.12621312967605017	0.0
2	2.5075456227029836E-4	0.0	0.0	0.5530809795141881	0.0
3	2.5075456227029836E-4	0.0	0.0	0.9954120273589945	0.0
4	2.5075456227029836E-4	0.0	0.0	1.5961363737045393	0.0
5	2.5075456227029836E-4	0.0	0.0	3.1682003094311297	0.0
6	2.5075456227029836E-4	0.0	0.0	5.040751795611628	0.0
7	2.5075456227029836E-4	0.0	0.0	6.406611896297943	0.0
8	2.5075456227029836E-4	0.0	0.0	8.239209822223375	0.0
9	2.5075456227029836E-4	0.0	0.0	9.483788299624955	0.0
10	2.5075456227029836E-4	0.0	0.0	11.319311695443538	0.0
11	2.5075456227029836E-4	0.0	0.0	12.796841161194227	0.0
12	2.5075456227029836E-4	0.0	0.0	14.055629063791125	0.0
13	2.5075456227029836E-4	0.0	0.0	14.663290953026149	0.0
14	3.3433941636039783E-4	0.0	0.0	15.031315065584856	0.0
15	3.3433941636039783E-4	0.0	0.0	15.350107699084495	0.0
16	3.3433941636039783E-4	0.0	0.0	15.720221432995455	0.0
17	3.3433941636039783E-4	0.0	0.0	16.130372312015574	0.0
18	3.3433941636039783E-4	0.0	0.0	16.64199520390107	0.0
19	5.015091245405967E-4	0.0	0.0	16.972573301827417	0.0
20	5.015091245405967E-4	0.0	0.0	17.29546159317747	0.0
21	5.015091245405967E-4	0.0	0.0	17.625371012271092	0.0
22	5.015091245405967E-4	0.0	0.0	18.006685116630127	0.0
23	5.015091245405967E-4	0.0	0.0	18.3885007301137	0.0
24	5.015091245405967E-4	0.0	0.0	18.703197705762925	0.0
25	5.015091245405967E-4	0.0	0.0	18.9786097999898	0.0
26	5.015091245405967E-4	0.0	0.0	19.256780194401653	0.0
27	5.015091245405967E-4	0.0	0.0	19.56654566365956	0.0
28	5.015091245405967E-4	0.0	0.0	19.857003031622657	0.0
29	5.015091245405967E-4	0.0	0.0	20.15356209393433	0.0
30	5.015091245405967E-4	0.0	0.0	20.54716317184461	0.0
31	5.015091245405967E-4	0.0	0.0	20.87632032725142	0.0
32	5.015091245405967E-4	0.0	0.0	21.19603239414605	0.0
33	5.850939786306962E-4	0.0	0.0	21.507051636215312	0.0
34	5.850939786306962E-4	0.0	0.0	21.81522899324551	0.0
35	5.850939786306962E-4	0.0	0.0	22.142714451570516	0.0
36	5.850939786306962E-4	0.0	0.0	22.458247275760645	0.0
37	5.850939786306962E-4	0.0	0.0	22.77612047586529	0.0
38	5.850939786306962E-4	0.0	0.0	23.152001564708467	0.0
39	6.686788327207957E-4	0.0	0.0	23.62467391458798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCGT	20	7.033402E-4	45.0	9
TATAGCG	40	6.8157533E-9	45.0	1
TATCGCG	20	7.033402E-4	45.0	1
CGTTTTT	6660	0.0	43.91892	1
CTATGCG	60	3.6379788E-12	41.249996	1
CATAGCG	50	1.0822987E-9	40.5	1
CGTTTTC	310	0.0	39.919353	1
CGTTATT	160	0.0	39.375	1
ACGGGTA	75	0.0	39.0	5
TATGCGG	215	0.0	37.67442	2
TTTAACG	60	1.5643309E-10	37.499996	1
CCGTAAG	30	1.13994996E-4	37.499996	1
GTTTTTT	7965	0.0	37.0339	2
TACGTAG	80	0.0	36.5625	1
GTACGAG	80	0.0	36.5625	1
CGTTTCT	465	0.0	36.290325	1
CGTAAGG	155	0.0	36.29032	2
CTCACGA	100	0.0	36.0	24
TAACGAG	25	0.0021071432	36.0	1
ATATACG	25	0.0021071432	36.0	1
>>END_MODULE