##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488565
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.913178389774135	33.0	31.0	34.0	30.0	34.0
2	31.95564561522008	33.0	31.0	34.0	30.0	34.0
3	31.998943845752358	33.0	31.0	34.0	30.0	34.0
4	35.68635493741877	37.0	35.0	37.0	33.0	37.0
5	34.792525047844194	37.0	35.0	37.0	33.0	37.0
6	35.26248298588724	37.0	35.0	37.0	33.0	37.0
7	35.86657456019158	37.0	35.0	37.0	35.0	37.0
8	35.850736340098045	37.0	35.0	37.0	35.0	37.0
9	37.66538945687882	39.0	38.0	39.0	35.0	39.0
10	37.27009916797151	39.0	37.0	39.0	34.0	39.0
11	37.17715350055776	39.0	37.0	39.0	34.0	39.0
12	37.209558605303286	39.0	37.0	39.0	34.0	39.0
13	37.15078444014614	39.0	37.0	39.0	33.0	39.0
14	38.26784153592664	40.0	38.0	41.0	33.0	41.0
15	38.351717785760336	40.0	38.0	41.0	34.0	41.0
16	38.36811069151495	40.0	38.0	41.0	34.0	41.0
17	38.31881939967047	40.0	37.0	41.0	34.0	41.0
18	38.335408799238586	40.0	37.0	41.0	34.0	41.0
19	38.23990052500691	40.0	37.0	41.0	34.0	41.0
20	38.23500660096405	40.0	37.0	41.0	34.0	41.0
21	38.19795523625311	40.0	37.0	41.0	34.0	41.0
22	38.25525774461945	40.0	37.0	41.0	34.0	41.0
23	38.24663248493036	40.0	37.0	41.0	34.0	41.0
24	38.2219684177131	40.0	37.0	41.0	34.0	41.0
25	38.09548166569443	40.0	37.0	41.0	34.0	41.0
26	38.1418296439573	40.0	37.0	41.0	34.0	41.0
27	38.14027406793364	40.0	37.0	41.0	34.0	41.0
28	38.02703018022167	40.0	37.0	41.0	34.0	41.0
29	38.02305118049799	40.0	37.0	41.0	34.0	41.0
30	37.91254797212244	40.0	37.0	41.0	34.0	41.0
31	37.838905775075986	40.0	37.0	41.0	33.0	41.0
32	37.794596420128336	40.0	37.0	41.0	33.0	41.0
33	37.65374515161749	40.0	36.0	41.0	33.0	41.0
34	37.58007225241268	40.0	36.0	41.0	33.0	41.0
35	37.528861052265306	40.0	36.0	41.0	33.0	41.0
36	37.48791870068466	40.0	36.0	41.0	33.0	41.0
37	37.437841433586115	40.0	36.0	41.0	33.0	41.0
38	37.37234963617942	40.0	36.0	41.0	33.0	41.0
39	37.25918352726863	40.0	36.0	41.0	32.0	41.0
40	37.1628360607084	39.0	36.0	41.0	32.0	41.0
41	37.070533091809686	39.0	35.0	41.0	31.0	41.0
42	37.17201600605856	39.0	36.0	41.0	32.0	41.0
43	37.10644233622957	39.0	35.0	41.0	32.0	41.0
44	36.98869546529121	39.0	35.0	41.0	31.0	41.0
45	36.97134465219572	39.0	35.0	41.0	31.0	41.0
46	36.80027631942526	39.0	35.0	41.0	31.0	41.0
47	36.68796987094859	39.0	35.0	41.0	31.0	41.0
48	36.680521527330036	39.0	35.0	41.0	31.0	41.0
49	36.682054588437566	39.0	35.0	41.0	31.0	41.0
50	36.55618802001781	39.0	35.0	41.0	31.0	41.0
51	35.40394829756532	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	3.0
11	6.0
12	1.0
13	5.0
14	8.0
15	7.0
16	21.0
17	33.0
18	58.0
19	174.0
20	329.0
21	659.0
22	888.0
23	1280.0
24	1829.0
25	2403.0
26	3239.0
27	3702.0
28	4394.0
29	5225.0
30	6558.0
31	8841.0
32	11393.0
33	16263.0
34	26712.0
35	36805.0
36	34805.0
37	53871.0
38	99357.0
39	169571.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06966319732277	19.864705822152633	23.423904700500444	15.641726280024152
2	26.9614073869393	27.68352215160726	27.51015729739134	17.844913164062103
3	29.305414837329728	27.089742408891343	28.536428110896196	15.068414642882727
4	26.387277025574896	26.431692814671536	29.41962686643538	17.761403293318185
5	26.180139797161072	29.75837401369316	25.90852803618761	18.152958152958153
6	23.94031500414479	34.734170478851325	25.081002527811037	16.24451198919284
7	79.43733177775731	4.245903820371906	11.370851370851371	4.945913031019414
8	80.05157962604771	5.618494980197108	8.388648388648388	5.941277005106793
9	74.08615025636303	6.279000747085854	11.230235485554635	8.404613510996489
10	33.85793087920747	35.66403651510034	16.99282592899614	13.485206676696038
11	24.290319609468547	26.02253538423751	31.114590689058772	18.572554317235166
12	22.37839386775557	22.513892726658685	34.44065784491316	20.66705556067258
13	21.34987156263752	27.376909930101416	34.544021778064334	16.72919672919673
14	17.986347773581816	31.145088166364758	29.92375630673503	20.944807753318393
15	15.673656524720355	26.932752039135018	38.250795697604204	19.142795738540418
16	18.07026700643722	28.70201508499381	31.122573250232826	22.105144658336148
17	18.268602949454014	26.935208211803957	30.17735613480294	24.618832703939088
18	21.593851381085425	24.49356789782322	32.557592132060215	21.354988589031144
19	19.48461310163438	28.635289060820973	29.405503873588977	22.474593963955666
20	21.86648654733761	27.079098993992613	30.848095954478932	20.206318504190847
21	20.4601230133145	30.558676941655666	29.571090847686595	19.41010919734324
22	18.91785125827679	25.89686121601015	29.490855873834597	25.694431651878457
23	16.34214485278315	29.888346484091166	29.432931135058794	24.33657752806689
24	19.238586472629027	25.72513381024019	31.347517730496456	23.688761986634326
25	16.84647897413855	30.739614994934144	28.954591507782997	23.45931452314431
26	17.877662132981282	27.19515315259996	29.874223491244766	25.05296122317399
27	19.513268449438662	27.203340394829755	29.629629629629626	23.653761526101952
28	16.079539058262462	26.85620132428643	33.382661467767846	23.681598149683257
29	19.3456346647836	24.192686745878238	29.561470838066583	26.900207751271584
30	20.30702158361733	25.521066797662545	32.23030712392415	21.941604494795985
31	20.913491551789424	26.00861707244686	29.903902244327774	23.173989131435942
32	22.133595325084688	26.46771668048264	27.493168769764516	23.90551922466816
33	20.84410467389191	25.985488113147685	29.28023906747311	23.890168145487294
34	18.796680073275816	24.03057934972829	32.84578305854902	24.32695751844688
35	18.595069233367106	24.41026270813505	30.257591108654943	26.73707694984291
36	19.788973831527024	27.06784153592664	30.136010561542477	23.00717407100386
37	19.17718215590556	25.474604198008453	33.9109432726454	21.437270373440587
38	20.119124374443523	27.131497344263302	29.662583279604554	23.086795001688618
39	22.72655634357762	25.22612139633416	30.285632413291985	21.76168984679623
40	23.032145159804735	23.36372847011145	30.40168657189934	23.20243979818448
41	17.281630898652175	26.1971283247879	29.625740689570478	26.89550008698945
42	19.83031940478749	24.253477019434467	30.34560396262524	25.570599613152805
43	21.449346555729534	24.891058508079787	30.34478523840226	23.314809697788423
44	21.0825581038347	24.380174592940552	30.02261725665981	24.514650046564938
45	21.55148240254623	22.649800947673288	28.909356994463376	26.8893596553171
46	23.357792719494846	23.762037804590996	29.04239968069755	23.837769795216605
47	17.807661211916532	24.025462323334665	33.99690931605826	24.169967148690553
48	19.591456612733207	23.906133267835393	30.58958378107314	25.91282633835825
49	21.3826205315567	21.46960998024828	33.20684044088299	23.940929047312025
50	20.833051896881685	21.995230931401146	31.91468893596553	25.257028235751637
51	19.435489648255604	21.017469528107828	28.94886043822214	30.598180385414427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	278.0
2	425.0
3	994.5
4	1564.0
5	1139.5
6	715.0
7	748.5
8	782.0
9	798.5
10	815.0
11	860.0
12	905.0
13	930.5
14	956.0
15	977.5
16	999.0
17	917.5
18	836.0
19	996.0
20	1156.0
21	1354.5
22	1553.0
23	1729.5
24	1906.0
25	2259.5
26	3527.5
27	4442.0
28	5113.5
29	5785.0
30	7349.0
31	8913.0
32	10127.5
33	11342.0
34	13128.5
35	14915.0
36	16499.0
37	18083.0
38	19434.0
39	20785.0
40	22689.5
41	24594.0
42	28220.5
43	31847.0
44	36364.0
45	40881.0
46	53383.0
47	65885.0
48	64269.0
49	62653.0
50	59877.5
51	57102.0
52	46998.0
53	36894.0
54	30709.0
55	24524.0
56	20007.5
57	15491.0
58	12799.0
59	10107.0
60	8415.5
61	6724.0
62	5704.0
63	4684.0
64	3762.5
65	2841.0
66	2315.5
67	1790.0
68	1374.5
69	959.0
70	754.0
71	549.0
72	531.0
73	513.0
74	452.5
75	386.0
76	380.0
77	212.5
78	45.0
79	30.5
80	16.0
81	41.0
82	66.0
83	36.0
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	488565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.005056096047426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41713644724173	28.28227445184637
2	10.894172221323206	8.2806725278278
3	4.026998875778141	4.5913895451800455
4	1.952398457003754	2.9680405152105642
5	1.2136714080671662	2.306282497288076
6	0.8467543373297433	1.930856765387301
7	0.6943663385434706	1.8472602153286162
8	0.545992075923484	1.6600367578775514
9	0.46916528635096383	1.604758772347788
>10	4.78015751863521	35.94807818721047
>50	0.09962725925093116	2.5459018065648458
>100	0.05143798711325197	3.1827888168556537
>500	0.004873072463360713	1.2906479252161802
>1k	0.0032487149755738086	3.5610112158587373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	3733	0.7640743810956577	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	3398	0.6955062274211211	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	3230	0.6611198100559803	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2889	0.5913235700469743	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2504	0.5125213635851934	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1551	0.31746031746031744	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	969	0.19833594301679408	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	848	0.17356953527166294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.1713180436584692	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT	708	0.14491418746737894	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	623	0.1275162977290637	No Hit
ACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	617	0.12628821139459437	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC	598	0.12239927133544154	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGGTCAT	537	0.10991372693500354	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTA	535	0.10950436482351376	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3324020345296941	0.0
2	0.0	0.0	0.0	1.8210473529622466	0.0
3	0.0	0.0	0.0	2.4256751916326382	0.0
4	0.0	0.0	0.0	3.3749859281774177	0.0
5	0.0	0.0	0.0	5.894814405452704	0.0
6	0.0	0.0	0.0	7.4657415082947	0.0
7	0.0	0.0	0.0	8.870672274927594	0.0
8	0.0	0.0	0.0	11.154708175984771	0.0
9	0.0	0.0	0.0	12.032585224074586	0.0
10	0.0	0.0	0.0	14.478933202337458	0.0
11	0.0	0.0	0.0	16.51591906911056	0.0
12	0.0	0.0	0.0	19.097356544165056	0.0
13	0.0	0.0	0.0	19.913419913419915	0.0
14	0.0	0.0	0.0	20.27140707991772	0.0
15	0.0	0.0	0.0	20.854748088790643	0.0
16	0.0	0.0	0.0	21.827801827801828	0.0
17	0.0	0.0	0.0	23.18319977894446	0.0
18	0.0	0.0	0.0	24.80550182677842	0.0
19	0.0	0.0	0.0	25.759724908661077	0.0
20	0.0	0.0	0.0	26.604648306775967	0.0
21	4.0936211148977106E-4	0.0	0.0	27.531239446133064	0.0
22	6.140431672346566E-4	0.0	0.0	28.56631154503495	0.0
23	6.140431672346566E-4	0.0	0.0	29.62041898212111	0.0
24	8.187242229795421E-4	0.0	0.0	30.356861420691207	0.0
25	8.187242229795421E-4	0.0	0.0	31.01470633385527	0.0
26	0.0010234052787244277	0.0	0.0	31.664568685845282	0.0
27	0.0010234052787244277	0.0	0.0	32.33899276452468	0.0
28	0.0010234052787244277	0.0	0.0	32.978621063727445	0.0
29	0.0010234052787244277	0.0	0.0	33.679858360709424	0.0
30	0.0010234052787244277	0.0	0.0	34.64165464165464	0.0
31	0.0010234052787244277	0.0	0.0	35.318739574058725	0.0
32	0.0010234052787244277	0.0	0.0	35.98129215150492	0.0
33	0.0010234052787244277	0.0	0.0	36.63279195194089	0.0
34	0.0012280863344693131	0.0	0.0	37.2306653157717	0.0
35	0.0012280863344693131	0.0	0.0	37.911229826123446	0.0
36	0.0012280863344693131	0.0	0.0	38.511559362623196	0.0
37	0.0012280863344693131	0.0	0.0	39.08016333548248	0.0
38	0.0012280863344693131	0.0	0.0	39.68908947632352	0.0
39	0.0012280863344693131	0.0	0.0	40.33076458608374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	20	7.029301E-4	45.0	27
ACGCCGC	20	7.029301E-4	45.0	14
TTAATCG	20	7.029301E-4	45.0	20
TCCGAGG	35	1.21001E-7	45.0	2
CGTTGGA	20	7.029301E-4	45.0	33
GCGCCCT	20	7.029301E-4	45.0	32
TCGATCA	40	6.8012014E-9	45.0	17
GCCGCCG	20	7.029301E-4	45.0	16
TGTTGCG	35	1.21001E-7	45.0	1
CTCGCGG	20	7.029301E-4	45.0	2
ACTCGCG	20	7.029301E-4	45.0	1
ACATGCG	25	3.8875012E-5	45.0	1
GCGGAAT	20	7.029301E-4	45.0	5
GTAAGCG	25	3.8875012E-5	45.0	1
CGCCGCA	20	7.029301E-4	45.0	18
TGTCGTC	35	1.21001E-7	45.0	17
TGTCGCC	20	7.029301E-4	45.0	33
TGAGACG	25	3.8875012E-5	45.0	1
CTAAGCG	30	2.1629294E-6	44.999996	1
CGGTAAT	30	2.1629294E-6	44.999996	6
>>END_MODULE