##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	590086
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03595408126951	33.0	31.0	34.0	30.0	34.0
2	32.057039143446886	33.0	31.0	34.0	30.0	34.0
3	32.113569886423335	33.0	31.0	34.0	30.0	34.0
4	35.77938808919378	37.0	35.0	37.0	35.0	37.0
5	34.86259799419068	37.0	35.0	37.0	33.0	37.0
6	35.32074985680053	37.0	35.0	37.0	33.0	37.0
7	35.863518199042176	37.0	35.0	37.0	35.0	37.0
8	35.8298061638473	37.0	35.0	37.0	35.0	37.0
9	37.65133895737232	39.0	37.0	39.0	35.0	39.0
10	37.37543680073752	39.0	37.0	39.0	34.0	39.0
11	37.31668943170995	39.0	37.0	39.0	34.0	39.0
12	37.18728795463712	39.0	37.0	39.0	34.0	39.0
13	37.06507187087984	39.0	37.0	39.0	33.0	39.0
14	38.14829872255908	40.0	37.0	41.0	33.0	41.0
15	38.22878021169796	40.0	37.0	41.0	33.0	41.0
16	38.28749368736082	40.0	37.0	41.0	34.0	41.0
17	38.243464511952496	40.0	37.0	41.0	34.0	41.0
18	38.23302535562613	40.0	37.0	41.0	34.0	41.0
19	38.14047274465078	40.0	37.0	41.0	34.0	41.0
20	38.122394023921935	40.0	37.0	41.0	34.0	41.0
21	38.10195124100555	40.0	37.0	41.0	34.0	41.0
22	38.1376053659975	40.0	37.0	41.0	34.0	41.0
23	38.13068434092658	40.0	37.0	41.0	34.0	41.0
24	38.10665055602065	40.0	36.0	41.0	34.0	41.0
25	37.97197866073759	40.0	36.0	41.0	34.0	41.0
26	37.941493273861774	40.0	36.0	41.0	34.0	41.0
27	37.940157875292755	40.0	36.0	41.0	34.0	41.0
28	37.81055812203645	40.0	36.0	41.0	33.0	41.0
29	37.75530176957257	40.0	36.0	41.0	33.0	41.0
30	37.66622492314679	40.0	36.0	41.0	33.0	41.0
31	37.5730859569622	40.0	36.0	41.0	33.0	41.0
32	37.50867500669394	40.0	36.0	41.0	33.0	41.0
33	37.353102768071096	40.0	35.0	41.0	33.0	41.0
34	37.25405788308823	40.0	35.0	41.0	32.0	41.0
35	37.2279447402582	40.0	35.0	41.0	32.0	41.0
36	37.05134166884149	39.0	35.0	41.0	32.0	41.0
37	37.030078666499456	39.0	35.0	41.0	32.0	41.0
38	36.93680751619255	39.0	35.0	41.0	32.0	41.0
39	36.8894364550252	39.0	35.0	41.0	32.0	41.0
40	36.8033778127256	39.0	35.0	41.0	31.0	41.0
41	36.63199940347678	39.0	35.0	41.0	31.0	41.0
42	36.659769931840444	39.0	35.0	41.0	31.0	41.0
43	36.500681256630386	39.0	35.0	41.0	31.0	41.0
44	36.43579918859285	39.0	35.0	41.0	31.0	41.0
45	36.428817155465474	39.0	35.0	41.0	31.0	41.0
46	36.321868337835504	38.0	35.0	40.0	31.0	41.0
47	36.17841467175971	38.0	35.0	40.0	30.0	41.0
48	36.128671075063636	38.0	35.0	40.0	30.0	41.0
49	36.13749012855753	38.0	35.0	40.0	30.0	41.0
50	35.98063672074918	38.0	35.0	40.0	30.0	41.0
51	34.83358527401091	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	12.0
11	17.0
12	9.0
13	9.0
14	18.0
15	30.0
16	40.0
17	47.0
18	150.0
19	272.0
20	514.0
21	898.0
22	1351.0
23	1776.0
24	2478.0
25	3150.0
26	4006.0
27	4830.0
28	5574.0
29	6700.0
30	8647.0
31	11126.0
32	15228.0
33	21261.0
34	38124.0
35	49533.0
36	45367.0
37	67336.0
38	115801.0
39	185623.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.956836800059655	17.58760587439797	21.380612317526598	16.07494500801578
2	28.992553627776292	27.18434262124504	25.492046921974087	18.331056829004584
3	31.01954630342018	27.096219873035455	25.861654063983895	16.02257975956047
4	27.626989964174715	24.85739366804161	28.719881508797023	18.795734858986656
5	26.604935551767028	29.998678158776855	24.447114488396608	18.949271801059506
6	25.888938222564168	35.1857186918517	23.70027419732039	15.225068888263745
7	76.66746880963113	6.0730130862281095	12.19110434750189	5.068413756638863
8	76.84100283687462	7.562626464617021	9.470653430177974	6.125717268330379
9	70.69139074643357	6.912721196571347	12.420901360140725	9.974986696854357
10	39.250380453018714	26.531556417200203	18.033812020620722	16.18425110916036
11	30.875838437109167	24.74774863325007	25.648803733693054	18.727609195947707
12	27.6846086841579	20.924916029188967	29.794809570130454	21.595665716522678
13	25.34901692295699	23.781957206237735	32.37680609267124	18.492219778134032
14	20.43159810603878	28.674125466457433	29.273360154282596	21.62091627322119
15	18.740319207708705	24.454232094982764	36.433502913134696	20.371945784173832
16	21.958663652416767	24.292391278559396	30.016472175242253	23.732472893781583
17	22.148127561067373	24.52795016319655	28.323329141853897	25.00059313388218
18	24.69521391797128	22.727704097368857	29.862765766345923	22.71431621831394
19	23.60757584487685	25.545090037723316	27.277549374159022	23.569784743240817
20	25.418159386936818	25.400365370471423	28.553804021786654	20.6276712208051
21	24.20223492846805	27.5881820615978	28.224360516941598	19.985222492992545
22	22.97190579000349	23.49843921055575	28.127594960734537	25.40206003870622
23	20.91085028284013	27.48124849598194	27.944231857729214	23.663669363448715
24	22.498076551553503	23.86414861562552	28.91053168521199	24.727243147608995
25	21.36942750717692	28.095735197920302	26.824056154526627	23.71078114037615
26	20.779174560996193	25.8184400239965	28.079635849689705	25.322749565317597
27	23.868046352565557	25.168365289127347	27.16892114030836	23.794667217998732
28	19.928790040773716	26.838799768169388	29.270648685106913	23.96176150594998
29	21.658876841680705	26.373952271363837	28.01439112264992	23.952779764305543
30	25.033300230813815	26.002142060648787	27.716637913795616	21.247919794741783
31	26.02247807946638	26.360055991838475	24.678436702446763	22.93902922624838
32	25.443071009988376	27.207390109238318	26.365139996542876	20.984398884230433
33	25.76675264283511	24.342214524662506	26.313961015851927	23.577071816650456
34	25.41697311917246	25.77742905271435	27.59970580559444	21.205892022518753
35	25.477642241978288	25.089563216209164	26.790840657124555	22.641953884687997
36	26.35802238995672	27.58021712089424	26.503933324972973	19.55782716417607
37	25.09312201950224	25.380876685771227	29.138295095969063	20.387706198757467
38	24.3862758987673	27.267889765220666	26.421064048291264	21.924770287720776
39	25.73607914778524	22.592808505878804	28.857827503109718	22.813284843226242
40	28.552787220845776	22.313018780313378	27.704775236152017	21.42941876268883
41	24.17020569883034	24.130889395782987	27.180444884305	24.518460021081673
42	24.724362211609833	23.70179939873171	28.991197893188453	22.582640496470006
43	24.308321159966514	23.631131733340563	29.023735523296608	23.036811583396318
44	23.847201933277525	23.748063841541743	28.402300681595566	24.002433543585173
45	23.165267435594135	22.494517748260424	28.031676738644872	26.308538077500565
46	26.462075019573415	23.727388889077186	27.164345536074403	22.646190555274995
47	22.635514145395756	23.9793860555919	31.482699131990934	21.902400667021418
48	22.98309060035317	24.22731601834309	28.53617947214474	24.253413909159004
49	23.75636771589226	21.68971980355406	31.75520178414672	22.798710696406964
50	23.191873726880488	22.314035581254256	30.19441233989622	24.299678351969035
51	22.450456374155632	22.389278850879364	27.17264941042492	27.987615364540087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	539.0
2	873.0
3	1524.0
4	2175.0
5	1549.5
6	924.0
7	908.5
8	893.0
9	937.0
10	981.0
11	973.5
12	966.0
13	997.0
14	1028.0
15	1097.5
16	1167.0
17	1134.5
18	1102.0
19	1202.0
20	1302.0
21	1256.5
22	1211.0
23	1550.0
24	1889.0
25	2079.5
26	3083.5
27	3897.0
28	4596.5
29	5296.0
30	6193.0
31	7090.0
32	8504.5
33	9919.0
34	11745.0
35	13571.0
36	14732.5
37	15894.0
38	16704.5
39	17515.0
40	20038.5
41	22562.0
42	25721.5
43	28881.0
44	33973.5
45	39066.0
46	53259.5
47	67453.0
48	64561.5
49	61670.0
50	61362.0
51	61054.0
52	53018.5
53	44983.0
54	40200.5
55	35418.0
56	33262.0
57	31106.0
58	28709.0
59	26312.0
60	23999.5
61	21687.0
62	19553.0
63	17419.0
64	15424.5
65	13430.0
66	11345.0
67	9260.0
68	8168.5
69	7077.0
70	5888.0
71	4699.0
72	4191.5
73	3684.0
74	2883.0
75	1628.5
76	1175.0
77	853.0
78	531.0
79	377.0
80	223.0
81	148.0
82	73.0
83	60.0
84	47.0
85	29.0
86	11.0
87	9.0
88	7.0
89	5.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	590086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.266171529406115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65856267222762	24.50338380463834
2	10.343741517057154	6.881933591245254
3	3.7408345525950932	3.733297316692726
4	1.9544732480092881	2.600713692716499
5	1.2215770583250887	2.031859597931487
6	0.8750276208678878	1.7465291357255572
7	0.7219684215623965	1.6811987745356485
8	0.6162465882954662	1.6400131768518629
9	0.485645330824234	1.4540004789888732
>10	6.191568613189302	43.62695406434298
>50	0.13713702432386674	2.896374422307307
>100	0.04656518363232869	2.723596283096862
>500	0.002558526573204873	0.6020850033321001
>1k	0.004093642517127797	3.8780606575945447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	4784	0.8107292835281638	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	4614	0.7819199235365693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	4459	0.7556525658971743	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2818	0.4775575085665479	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2552	0.43247933352087664	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1223	0.20725792511600005	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1195	0.20251285405856095	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	1137	0.19268377829672284	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	886	0.15014760560325105	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	691	0.11710157502465741	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	675	0.11439010584897795	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	648	0.10981450161501882	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	637	0.10795036655673919	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6946682347996733E-4	0.0	0.0	0.2204763373474375	0.0
2	1.6946682347996733E-4	0.0	0.0	1.3857302155956928	0.0
3	1.6946682347996733E-4	0.0	0.0	1.8473578427551238	0.0
4	1.6946682347996733E-4	0.0	0.0	2.5547123639605074	0.0
5	1.6946682347996733E-4	0.0	0.0	4.659659778405182	0.0
6	1.6946682347996733E-4	0.0	0.0	5.930322020857977	0.0
7	1.6946682347996733E-4	0.0	0.0	7.046430520297042	0.0
8	1.6946682347996733E-4	0.0	0.0	8.641452262890494	0.0
9	1.6946682347996733E-4	0.0	0.0	9.291526997759648	0.0
10	1.6946682347996733E-4	0.0	0.0	11.287168311059744	0.0
11	1.6946682347996733E-4	0.0	0.0	12.691878810885193	0.0
12	1.6946682347996733E-4	0.0	0.0	14.78309941262799	0.0
13	1.6946682347996733E-4	0.0	0.0	15.362337015282518	0.0
14	1.6946682347996733E-4	0.0	0.0	15.635348067908746	0.0
15	1.6946682347996733E-4	0.0	0.0	16.13934240093817	0.0
16	1.6946682347996733E-4	0.0	0.0	16.890249895777902	0.0
17	3.3893364695993466E-4	0.0	0.0	17.758258965642295	0.0
18	3.3893364695993466E-4	0.0	0.0	18.813189941805092	0.0
19	3.3893364695993466E-4	0.0	0.0	19.51241005548344	0.0
20	3.3893364695993466E-4	0.0	0.0	20.143335039299355	0.0
21	3.3893364695993466E-4	0.0	0.0	20.821032866395747	0.0
22	3.3893364695993466E-4	0.0	0.0	21.51957511278017	0.0
23	3.3893364695993466E-4	0.0	0.0	22.166260511179726	0.0
24	3.3893364695993466E-4	0.0	0.0	22.710587948197382	0.0
25	3.3893364695993466E-4	0.0	0.0	23.206786807346727	0.0
26	3.3893364695993466E-4	0.0	0.0	23.674515240151436	0.0
27	3.3893364695993466E-4	0.0	0.0	24.202743328938492	0.0
28	3.3893364695993466E-4	0.0	0.0	24.677928301976323	0.0
29	3.3893364695993466E-4	0.0	0.0	25.190565443003223	0.0
30	3.3893364695993466E-4	0.0	0.0	25.88520995244761	0.0
31	3.3893364695993466E-4	0.0	0.0	26.391746287829232	0.0
32	3.3893364695993466E-4	0.0	0.0	26.914043037794492	0.0
33	3.3893364695993466E-4	0.0	0.0	27.404818958592475	0.0
34	3.3893364695993466E-4	0.0	0.0	27.889494073745183	0.0
35	3.3893364695993466E-4	0.0	0.0	28.446362055700355	0.0
36	3.3893364695993466E-4	0.0	0.0	28.919174493209464	0.0
37	3.3893364695993466E-4	0.0	0.0	29.41486495188837	0.0
38	3.3893364695993466E-4	0.0	0.0	29.912250078802074	0.0
39	5.08400470439902E-4	0.0	0.0	30.532329185915273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGTT	20	7.0304924E-4	45.0	30
GAACGTA	20	7.0304924E-4	45.0	1
CTATTCG	20	7.0304924E-4	45.0	26
TTACGTC	20	7.0304924E-4	45.0	18
CGGTCTA	40	6.8048394E-9	45.0	31
ACTCAAC	20	7.0304924E-4	45.0	39
CAATAGA	40	6.8048394E-9	45.0	12
CGTAAGG	90	0.0	40.0	2
ATTAGCG	40	3.4544064E-7	39.375	1
GCGATAC	40	3.4544064E-7	39.375	9
CGGGCTC	110	0.0	38.86364	6
TTGCACG	35	6.243121E-6	38.57143	1
CCCGCAT	35	6.243121E-6	38.57143	42
ACACGGT	30	1.1392485E-4	37.500004	36
GCGCGAA	30	1.1392485E-4	37.500004	12
AGTTACG	30	1.1392485E-4	37.500004	1
CGATACG	30	1.1392485E-4	37.500004	10
TCGCAGG	55	2.744855E-9	36.818184	2
TACGGCT	695	0.0	36.582733	7
AGGGCGC	125	0.0	36.000004	6
>>END_MODULE