##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935738.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413544
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01344351502323	33.0	31.0	34.0	30.0	34.0
2	32.04311432824164	33.0	31.0	34.0	30.0	34.0
3	32.07590142224083	33.0	31.0	34.0	30.0	34.0
4	35.74198256297646	37.0	35.0	37.0	33.0	37.0
5	34.80738908728699	37.0	35.0	37.0	33.0	37.0
6	35.294344569394376	37.0	35.0	37.0	33.0	37.0
7	35.88633675357824	37.0	35.0	37.0	35.0	37.0
8	35.87338774031795	37.0	35.0	37.0	35.0	37.0
9	37.7052189390638	39.0	38.0	39.0	35.0	39.0
10	37.287924535776746	39.0	37.0	39.0	34.0	39.0
11	37.26313294810773	39.0	37.0	39.0	34.0	39.0
12	37.34051504587052	39.0	37.0	39.0	34.0	39.0
13	37.355768196815944	39.0	37.0	39.0	34.0	39.0
14	38.5391017188004	40.0	38.0	41.0	34.0	41.0
15	38.58675004103162	40.0	38.0	41.0	34.0	41.0
16	38.59499881149791	40.0	38.0	41.0	34.0	41.0
17	38.539016825793894	40.0	38.0	41.0	34.0	41.0
18	38.49382474121782	40.0	38.0	41.0	34.0	41.0
19	38.41580240869757	40.0	38.0	41.0	34.0	41.0
20	38.39848211304353	40.0	38.0	41.0	34.0	41.0
21	38.360098447589884	40.0	38.0	41.0	34.0	41.0
22	38.41883804112217	40.0	38.0	41.0	34.0	41.0
23	38.40510447499335	40.0	38.0	41.0	34.0	41.0
24	38.38047772124532	40.0	38.0	41.0	34.0	41.0
25	38.24321209668747	40.0	37.0	41.0	34.0	41.0
26	38.28682800110927	40.0	37.0	41.0	34.0	41.0
27	38.29070478174008	40.0	38.0	41.0	34.0	41.0
28	38.149985426700546	40.0	37.0	41.0	34.0	41.0
29	38.09022570220665	40.0	37.0	41.0	34.0	41.0
30	37.98644329430142	40.0	37.0	41.0	33.0	41.0
31	37.98249718438195	40.0	37.0	41.0	34.0	41.0
32	37.930147911915014	40.0	37.0	41.0	33.0	41.0
33	37.79814706864448	40.0	37.0	41.0	33.0	41.0
34	37.73126835811266	40.0	37.0	41.0	33.0	41.0
35	37.68756685324263	40.0	37.0	41.0	33.0	41.0
36	37.62800450498888	40.0	37.0	41.0	33.0	41.0
37	37.60783534152456	40.0	37.0	41.0	33.0	41.0
38	37.53673178903522	40.0	37.0	41.0	33.0	41.0
39	37.4546989693989	40.0	37.0	41.0	33.0	41.0
40	37.35970864720164	40.0	37.0	41.0	32.0	41.0
41	37.25777902916358	40.0	36.0	41.0	32.0	41.0
42	37.29057744223031	40.0	36.0	41.0	32.0	41.0
43	37.19966198434573	40.0	36.0	41.0	32.0	41.0
44	37.11324090371435	40.0	36.0	41.0	31.0	41.0
45	37.101070783788835	40.0	36.0	41.0	31.0	41.0
46	36.9634344597692	40.0	36.0	41.0	31.0	41.0
47	36.879192299638355	39.0	35.0	41.0	31.0	41.0
48	36.84153446939041	39.0	35.0	41.0	31.0	41.0
49	36.81712490025072	39.0	35.0	41.0	31.0	41.0
50	36.699030946330645	39.0	35.0	41.0	31.0	41.0
51	35.558136145744314	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	19.0
10	25.0
11	20.0
12	26.0
13	20.0
14	28.0
15	37.0
16	57.0
17	97.0
18	225.0
19	469.0
20	961.0
21	1620.0
22	2689.0
23	4059.0
24	5781.0
25	8209.0
26	10628.0
27	12122.0
28	12884.0
29	14754.0
30	17857.0
31	23406.0
32	31114.0
33	41895.0
34	66788.0
35	87533.0
36	96223.0
37	146272.0
38	275285.0
39	552060.0
40	375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90134866689682	18.067566343884593	22.435523761552524	17.595561227666064
2	28.16396235278138	27.69259393411171	26.901815578432647	17.241628134674265
3	29.90235889367434	26.26596695964186	28.07708850944859	15.754585637235206
4	26.283440770149358	25.007215905553696	30.68620432048808	18.023139003808865
5	25.32690881925147	30.620765961300105	25.901705217524178	18.150620001924242
6	24.719711590159203	34.93743385419909	25.76198547763635	14.580869078005353
7	79.69090456328207	4.672723311053635	11.28468586757823	4.351686258086059
8	80.8822364213636	5.706861618739848	8.470128980774563	4.94077297912198
9	74.09001771434069	6.859567158857454	10.999869830723345	8.050545296078509
10	32.64065356295948	35.17173855217807	18.006938588399088	14.180669296463359
11	23.414057149971985	26.730331705274118	31.24225351315559	18.61335763159831
12	21.070373472633324	22.985418211247758	34.55789137090887	21.386316945210055
13	21.805759141561918	24.59590928899277	36.26126954661475	17.33706202283056
14	19.55220353947242	29.309169010656905	31.205749520354516	19.932877929516167
15	17.869341173674115	27.01026639425444	36.39179254413021	18.72859988794123
16	20.59907579813575	27.852051298014068	30.405703678130998	21.143169225719184
17	21.261453481462198	26.423372742553468	30.267964775061827	22.047209000922503
18	22.3751789827554	25.156344620330177	31.229731794694754	21.23874460221967
19	20.86224411832953	28.462149038162238	29.198171404639684	21.477435438868547
20	21.239664276456903	27.749684480992453	30.90791655583413	20.10273468671651
21	21.33106574680378	29.376871183351916	30.479631337970375	18.81243173187393
22	20.059156276705924	25.411377360733024	30.683516041948465	23.845950320612587
23	18.572538244299437	28.637806817474377	30.338850435501126	22.450804502725067
24	19.617075945283627	26.153766702699034	30.763881421448502	23.46527593056884
25	18.6134991199425	30.341538714040738	28.924532946975827	22.120429219040936
26	19.299010147544045	27.271666110145848	30.564099879451927	22.865223862858176
27	21.467672743119422	26.729058310176406	28.817214037907558	22.986054908796614
28	18.85077507314947	26.66736939210948	32.043006797100055	22.438848737640992
29	20.1533875139366	24.37780500642357	31.87633352764399	23.592473951995835
30	21.551363098707927	25.815963280944914	30.49201156808702	22.140662052260137
31	20.84144533173357	25.83952109025259	28.54873990480664	24.7702936732072
32	20.639612208746243	26.3246846224808	30.91470799635526	22.120995172417697
33	19.840415296587867	24.493118006938587	29.82121532828126	25.84525136819229
34	18.86570209346154	24.197619600097344	31.372988743187335	25.563689563253778
35	19.213480443481064	24.203420622209144	32.553638231282505	24.029460703027283
36	19.123776833264476	24.535069300990983	32.3833570090496	23.957796856694948
37	19.428189005789704	24.748716700718195	32.91301862552563	22.910075667966474
38	18.28354830129094	25.064801661639112	31.01700406920478	25.634645967865165
39	20.02470386489561	23.48692364722994	32.12252324653495	24.3658492413395
40	20.32444692206256	23.412854499046368	32.907076115069636	23.35562246382143
41	18.568293593973728	24.73287000616889	31.55020289428557	25.14863350557181
42	20.12381645000085	24.21155620200008	33.507128182780306	22.15749916521877
43	20.925489408182553	24.170312349668635	32.00699801350365	22.897200228645165
44	20.104149570158413	24.13380835686756	30.386814984181605	25.375227088792425
45	19.56734279230077	23.28367564080071	30.05643970049747	27.092541866401042
46	22.21699501395075	24.171444256422156	29.85743634439395	23.754124385233144
47	19.05565019553689	23.215902723933603	33.38969285710243	24.338754223427074
48	19.642473103065768	23.68698816591489	31.475426304381042	25.195112426638293
49	20.25674474936755	21.989835477353374	33.932654377932344	23.820765395346733
50	19.795917212340047	22.19110264696394	32.91804146174438	25.094938678951628
51	19.534376008104452	21.711740136847528	30.375708149162673	28.37817570588535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	692.0
2	1139.0
3	4272.5
4	7406.0
5	5196.5
6	2987.0
7	2876.5
8	2766.0
9	2936.5
10	3107.0
11	3071.5
12	3036.0
13	3108.0
14	3180.0
15	3075.5
16	2971.0
17	3006.5
18	3042.0
19	3280.0
20	3518.0
21	3964.0
22	4410.0
23	5551.5
24	6693.0
25	7604.5
26	10619.0
27	12722.0
28	15932.5
29	19143.0
30	23150.5
31	27158.0
32	31150.5
33	35143.0
34	39154.0
35	43165.0
36	47932.0
37	52699.0
38	58423.0
39	64147.0
40	69744.0
41	75341.0
42	83024.0
43	90707.0
44	101269.0
45	111831.0
46	146251.5
47	180672.0
48	172062.0
49	163452.0
50	156908.0
51	150364.0
52	126675.0
53	102986.0
54	87736.0
55	72486.0
56	61753.0
57	51020.0
58	44084.5
59	37149.0
60	32143.0
61	27137.0
62	22283.0
63	17429.0
64	14260.0
65	11091.0
66	8641.5
67	6192.0
68	4907.5
69	3623.0
70	2680.5
71	1738.0
72	1565.5
73	1393.0
74	1045.0
75	596.5
76	496.0
77	407.0
78	318.0
79	227.5
80	137.0
81	89.0
82	41.0
83	31.5
84	22.0
85	15.5
86	9.0
87	7.0
88	5.0
89	6.0
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1413544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.27412960406201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62617113479705	23.439464598512274
2	10.519354358830014	6.790060118558691
3	4.031373253345087	3.903271885824253
4	2.1587134021512617	2.7868238447622184
5	1.3980119412701981	2.2559809290290356
6	1.028284659824431	1.9912195428625503
7	0.7534197130190498	1.7021175824962413
8	0.6412362254437751	1.6556272837433539
9	0.5445409857071213	1.5817127712691776
>10	5.98998272343751	41.04046500247113
>50	0.2574805802006046	5.105027953794644
>100	0.045393467779391995	2.536891448847021
>500	0.002683357419795396	0.6249851647639275
>1k	0.002459744301479113	1.8927091144455848
>5k	6.70839354948849E-4	1.6487772602998485
>10k+	2.23613118316283E-4	1.0448654983201089
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	14478	1.0242341235928984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	8951	0.6332310844232653	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	8257	0.5841346289892638	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	5638	0.3988556422721896	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	4656	0.32938486527479865	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4090	0.2893436638689705	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	3722	0.26330980853797264	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	2534	0.17926573208899052	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	2143	0.1516047607998053	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2095	0.1482090405392404	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	1861	0.13165490426898632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.659533767608224E-4	0.0	0.0	0.19008959041954124	0.0
2	5.659533767608224E-4	0.0	0.0	1.0325111917280254	0.0
3	5.659533767608224E-4	0.0	0.0	1.4767138483131759	0.0
4	5.659533767608224E-4	0.0	0.0	2.3053403360631153	0.0
5	5.659533767608224E-4	0.0	0.0	4.462683864103275	0.0
6	5.659533767608224E-4	0.0	0.0	5.938195061490834	0.0
7	5.659533767608224E-4	0.0	0.0	7.0716581867985715	0.0
8	5.659533767608224E-4	0.0	0.0	8.8596463923302	0.0
9	5.659533767608224E-4	0.0	0.0	9.624956846055023	0.0
10	6.366975488559252E-4	0.0	0.0	11.542123909832307	0.0
11	6.366975488559252E-4	0.0	0.0	12.879613227462322	0.0
12	6.366975488559252E-4	0.0	0.0	14.913366686852337	0.0
13	6.366975488559252E-4	0.0	0.0	15.621798826212697	0.0
14	7.074417209510281E-4	0.0	0.0	15.963493177432044	0.0
15	7.074417209510281E-4	0.0	0.0	16.415053227915084	0.0
16	7.074417209510281E-4	0.0	0.0	17.08860849043256	0.0
17	7.074417209510281E-4	0.0	0.0	17.952041110853287	0.0
18	7.074417209510281E-4	0.0	0.0	18.990777789725684	0.0
19	7.074417209510281E-4	0.0	0.0	19.696167929685952	0.0
20	7.074417209510281E-4	0.0	0.0	20.29961571765718	0.0
21	7.074417209510281E-4	0.0	0.0	20.984843768570347	0.0
22	7.074417209510281E-4	0.0	0.0	21.75072017567193	0.0
23	7.074417209510281E-4	0.0	0.0	22.473654870311783	0.0
24	7.074417209510281E-4	0.0	0.0	23.033382759928237	0.0
25	7.074417209510281E-4	0.0	0.0	23.552362006417912	0.0
26	7.074417209510281E-4	0.0	0.0	24.051391396376765	0.0
27	7.074417209510281E-4	0.0	0.0	24.613949052877025	0.0
28	7.074417209510281E-4	0.0	0.0	25.13349425274346	0.0
29	7.074417209510281E-4	0.0	0.0	25.708927348565027	0.0
30	7.074417209510281E-4	0.0	0.0	26.464899571573294	0.0
31	7.781858930461309E-4	0.0	0.0	27.055471920223212	0.0
32	8.489300651412337E-4	0.0	0.0	27.63621082895191	0.0
33	8.489300651412337E-4	0.0	0.0	28.209804576299003	0.0
34	8.489300651412337E-4	0.0	0.0	28.787147764767138	0.0
35	8.489300651412337E-4	0.0	0.0	29.371706858788972	0.0
36	9.196742372363365E-4	0.0	0.0	29.90278335870691	0.0
37	9.196742372363365E-4	0.0	0.0	30.42933223161076	0.0
38	9.196742372363365E-4	0.0	0.0	30.974699054291907	0.0
39	9.904184093314393E-4	0.0	0.0	31.5786420514678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	20	7.033837E-4	45.0	14
CCGCTAG	20	7.033837E-4	45.0	1
CGGATCG	20	7.033837E-4	45.0	2
CGAATAC	20	7.033837E-4	45.0	44
TTACGCG	20	7.033837E-4	45.0	1
ACCGTAG	20	7.033837E-4	45.0	42
TCGCGCA	20	7.033837E-4	45.0	34
CGTTTTT	2820	0.0	41.010635	1
GCCGATT	90	0.0	40.0	9
TACGCGG	85	0.0	39.705883	2
CGACCGG	40	3.458972E-7	39.375	2
TACGAAG	40	3.458972E-7	39.375	1
TGTTACG	80	0.0	39.375	1
ACGCTAG	40	3.458972E-7	39.375	1
CGTAAGG	200	0.0	39.375	2
CGAACGG	75	0.0	39.0	2
CTGTACG	75	0.0	39.0	1
ACTTCGA	35	6.2493073E-6	38.57143	29
GTAATCG	35	6.2493073E-6	38.57143	1
TTACGAG	35	6.2493073E-6	38.57143	1
>>END_MODULE