##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682579
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.137956192616535	33.0	31.0	34.0	30.0	34.0
2	32.173686855294406	33.0	31.0	34.0	30.0	34.0
3	32.20531396365842	34.0	31.0	34.0	30.0	34.0
4	35.839765067486695	37.0	35.0	37.0	35.0	37.0
5	34.93926124302095	37.0	35.0	37.0	33.0	37.0
6	35.39763162945241	37.0	35.0	37.0	33.0	37.0
7	35.94691749965938	37.0	35.0	37.0	35.0	37.0
8	35.92654037115118	37.0	35.0	37.0	35.0	37.0
9	37.76171988883338	39.0	38.0	39.0	35.0	39.0
10	37.440286032825504	39.0	37.0	39.0	35.0	39.0
11	37.381249642898474	39.0	37.0	39.0	35.0	39.0
12	37.272415354120184	39.0	37.0	39.0	34.0	39.0
13	37.21155646452645	39.0	37.0	39.0	34.0	39.0
14	38.28017855808632	40.0	38.0	41.0	33.0	41.0
15	38.386195590547025	40.0	38.0	41.0	34.0	41.0
16	38.4173524236755	40.0	38.0	41.0	34.0	41.0
17	38.383189345116094	40.0	38.0	41.0	34.0	41.0
18	38.35743408455285	40.0	37.0	41.0	34.0	41.0
19	38.24868916271963	40.0	37.0	41.0	34.0	41.0
20	38.23793143357765	40.0	37.0	41.0	34.0	41.0
21	38.15520254798346	40.0	37.0	41.0	34.0	41.0
22	38.20207624318943	40.0	37.0	41.0	34.0	41.0
23	38.191199846464656	40.0	37.0	41.0	34.0	41.0
24	38.1505378864571	40.0	37.0	41.0	34.0	41.0
25	37.9913270112324	40.0	36.0	41.0	34.0	41.0
26	38.013550079917486	40.0	36.0	41.0	34.0	41.0
27	38.01063027136786	40.0	36.0	41.0	34.0	41.0
28	37.88569235209404	40.0	36.0	41.0	34.0	41.0
29	37.838322011078574	40.0	36.0	41.0	33.0	41.0
30	37.711788672080445	40.0	36.0	41.0	33.0	41.0
31	37.65006101857807	40.0	36.0	41.0	33.0	41.0
32	37.569684974193464	40.0	36.0	41.0	33.0	41.0
33	37.417777282922565	40.0	35.0	41.0	33.0	41.0
34	37.312379958949805	40.0	35.0	41.0	33.0	41.0
35	37.23455600011134	40.0	35.0	41.0	32.0	41.0
36	37.15330386665866	40.0	35.0	41.0	32.0	41.0
37	37.10062278505492	40.0	35.0	41.0	32.0	41.0
38	36.98804680483871	39.0	35.0	41.0	32.0	41.0
39	36.89516231820786	39.0	35.0	41.0	31.0	41.0
40	36.75645163416982	39.0	35.0	41.0	31.0	41.0
41	36.637568691682574	39.0	35.0	41.0	31.0	41.0
42	36.63875683254246	39.0	35.0	41.0	31.0	41.0
43	36.551941973017044	39.0	35.0	41.0	31.0	41.0
44	36.43143138010399	39.0	35.0	41.0	31.0	41.0
45	36.36223059894898	39.0	35.0	41.0	31.0	41.0
46	36.23564744886672	38.0	35.0	41.0	30.0	41.0
47	36.11902651561211	38.0	35.0	40.0	30.0	41.0
48	36.04519769872791	38.0	35.0	40.0	30.0	41.0
49	36.00585426741813	38.0	35.0	40.0	30.0	41.0
50	35.87057029296243	38.0	35.0	40.0	30.0	41.0
51	34.70856706696221	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	19.0
10	11.0
11	14.0
12	14.0
13	17.0
14	16.0
15	25.0
16	50.0
17	85.0
18	162.0
19	295.0
20	566.0
21	944.0
22	1362.0
23	2028.0
24	2852.0
25	3970.0
26	5298.0
27	6204.0
28	6984.0
29	8114.0
30	10116.0
31	12564.0
32	16479.0
33	23496.0
34	42665.0
35	52022.0
36	50445.0
37	77177.0
38	131032.0
39	227366.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90375180015793	17.359162822178824	21.04840611855917	18.688679259104074
2	29.909211973998612	25.49800096399098	26.256008462024177	18.33677859998623
3	32.53542813359333	24.947881490640643	26.338929266795493	16.177761108970536
4	28.330786619570773	25.417570713426578	27.215604347628624	19.036038319374022
5	25.347102679689826	30.380952241425536	25.198108936841013	19.073836142043632
6	26.043139328927495	33.65793556496757	25.33684745648489	14.962077649620046
7	79.33147664958928	5.89543481413873	9.997377592923309	4.775710943348682
8	80.52415910832299	5.608435067589246	8.849818116291301	5.01758770779646
9	74.0645405147243	7.039038704677407	11.722892148747617	7.1735286318506715
10	39.31383766567679	27.531904731906494	17.639862931616708	15.51439467080001
11	30.51441664627831	24.97835415387816	25.45566154247347	19.051567657370065
12	26.24077213040542	22.347596395435545	30.546793850968168	20.86483762319087
13	25.357504406083397	23.146624786288474	32.04112637511555	19.454744432512573
14	21.432683982366875	25.84155094135624	31.63238247880465	21.093382597472235
15	20.45111261846614	23.777174510203213	34.811062162767975	20.96065070856267
16	24.80811744867627	24.110176257986254	29.7123116884639	21.369394604873577
17	24.39937355236537	24.36333376795946	28.155861812332343	23.081430867342828
18	26.83117998063228	22.607932561652206	29.094507741961007	21.466379715754513
19	24.788925530964182	26.277544430754535	26.86663375228362	22.066896285997665
20	25.619012597809192	26.425366148094216	27.101478363676584	20.85414289042001
21	25.10844898539217	26.662701313694093	28.382648748350007	19.846200952563734
22	24.01421666942581	23.599905651946514	28.749639235897966	23.636238442729706
23	22.527062801521875	25.73475011683629	28.806921982656952	22.93126509898488
24	23.00905829215373	24.56301761407837	29.339021563804334	23.088902529963566
25	23.7005533425435	26.427270689546557	26.839823668762154	23.03235229914779
26	23.004516693305828	24.878731985601664	27.23626129722713	24.88049002386537
27	22.84350968898838	23.971291235153732	28.673750584181462	24.511448491676422
28	21.275632564142757	25.919783644091016	28.93320773126627	23.87137606049996
29	22.51827261020336	25.19254181567262	27.61922063233706	24.669964941786958
30	23.632136353447734	23.77600248469408	30.140540508864177	22.45132065299401
31	24.97981918576458	23.726484406933118	28.561236135304487	22.73246027199782
32	25.437934656647805	23.746115834211132	27.745799387323665	23.070150121817402
33	24.320408333687382	23.734688585497064	28.296358370239926	23.648544710575624
34	22.19201000909785	23.936716482634242	30.213645599996486	23.657627908271422
35	23.141350671497367	25.143609750666222	28.638003806152838	23.077035771683573
36	24.068129842846027	26.361490757846344	27.95456643113837	21.61581296816925
37	23.195996360860793	26.502866334885777	27.695402290430852	22.605735013822574
38	23.485193655239904	26.740641010051586	28.35027154366015	21.42389379104836
39	23.85320966510836	24.5732728372833	27.1347345874983	24.43878291011004
40	25.274583601312084	23.664953067703518	28.848235881853967	22.21222744913043
41	21.541535851527808	25.206313115404953	27.92087069775074	25.331280335316496
42	22.99616601155324	24.87887848879031	28.60445457595385	23.520500923702603
43	22.67371249335242	24.066371804582325	29.603020309737044	23.656895392328213
44	23.216946316836587	24.025643918139878	28.945220992734903	23.81218877228863
45	22.947819959301412	23.012427865492494	28.37122149963594	25.668530675570157
46	23.864783417011072	23.642391576652667	27.917647627600616	24.57517737873565
47	21.80201852093311	24.833902009877246	30.017624333593623	23.346455135596024
48	22.233323908294864	24.84269220119576	29.321294677978667	23.602689212530713
49	23.33605340920245	23.664660061326234	29.318071607828543	23.68121492164277
50	21.554281628939652	23.893791048362168	30.55543753909804	23.99648978360014
51	21.58709834319544	22.772455642497057	28.563287179945473	27.07715883436203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	535.0
2	858.0
3	2859.0
4	4860.0
5	3122.0
6	1384.0
7	1397.0
8	1410.0
9	1423.5
10	1437.0
11	1483.5
12	1530.0
13	1539.0
14	1548.0
15	1589.0
16	1630.0
17	1669.0
18	1708.0
19	1644.0
20	1580.0
21	1735.0
22	1890.0
23	2361.0
24	2832.0
25	3360.0
26	4402.0
27	4916.0
28	6087.5
29	7259.0
30	8506.0
31	9753.0
32	11337.5
33	12922.0
34	15262.0
35	17602.0
36	18888.0
37	20174.0
38	22026.0
39	23878.0
40	25705.5
41	27533.0
42	29934.0
43	32335.0
44	36331.0
45	40327.0
46	48735.0
47	57143.0
48	58983.5
49	60824.0
50	61328.5
51	61833.0
52	56845.5
53	51858.0
54	47400.0
55	42942.0
56	40187.5
57	37433.0
58	35447.5
59	33462.0
60	30945.5
61	28429.0
62	26499.5
63	24570.0
64	22285.0
65	20000.0
66	16963.0
67	13926.0
68	11908.5
69	9891.0
70	8322.0
71	6753.0
72	5449.0
73	4145.0
74	3453.5
75	2271.5
76	1781.0
77	1191.0
78	601.0
79	553.5
80	506.0
81	325.0
82	144.0
83	108.0
84	72.0
85	46.0
86	20.0
87	14.5
88	9.0
89	6.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	682579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.293145589218035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5036187636598	24.863771540073465
2	8.864892110979763	6.080100715890789
3	3.474368483860802	3.574410727428877
4	2.0874725140011723	2.8634399534453276
5	1.531625516291435	2.626212840917171
6	1.214626467126495	2.4992017364211874
7	1.0743809671775264	2.5790732047992657
8	0.9128739905852661	2.504425653100079
9	0.8433803305024653	2.6029948014903486
>10	7.393829984549971	44.47356433676194
>50	0.06408999924610316	1.4641500680918285
>100	0.030539529794619973	1.8997369236102661
>500	0.001720536889837745	0.3815994581395884
>1k	0.0025808053347566176	1.5873180398299616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3015	0.4417071137553309	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	2488	0.3644999333410492	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	1385	0.20290691626903262	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	1385	0.20290691626903262	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1312	0.19221218349817384	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1176	0.17228774984287534	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	717	0.10504278625624286	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	692	0.10138020654019535	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.930063772838016E-4	0.0	0.0	0.11222144249969601	0.0
2	2.930063772838016E-4	0.0	0.0	0.5868917736994546	0.0
3	2.930063772838016E-4	0.0	0.0	0.9147659098800286	0.0
4	2.930063772838016E-4	0.0	0.0	1.342262214337095	0.0
5	2.930063772838016E-4	0.0	0.0	2.5156062521700786	0.0
6	2.930063772838016E-4	0.0	0.0	3.7088747236583606	0.0
7	2.930063772838016E-4	0.0	0.0	4.527095032223376	0.0
8	2.930063772838016E-4	0.0	0.0	5.868331724239978	0.0
9	2.930063772838016E-4	0.0	0.0	6.5377047931448224	0.0
10	2.930063772838016E-4	0.0	0.0	7.734928850726436	0.0
11	2.930063772838016E-4	0.0	0.0	8.71400966042026	0.0
12	2.930063772838016E-4	0.0	0.0	9.786852510844898	0.0
13	2.930063772838016E-4	0.0	0.0	10.23632429359825	0.0
14	2.930063772838016E-4	0.0	0.0	10.483914682403062	0.0
15	2.930063772838016E-4	0.0	0.0	10.777507072441432	0.0
16	2.930063772838016E-4	0.0	0.0	11.209544975746397	0.0
17	2.930063772838016E-4	0.0	0.0	11.70311421828096	0.0
18	2.930063772838016E-4	0.0	0.0	12.33586149002533	0.0
19	2.930063772838016E-4	0.0	0.0	12.704463512648353	0.0
20	2.930063772838016E-4	0.0	0.0	13.107933294168147	0.0
21	2.930063772838016E-4	0.0	0.0	13.491332138844001	0.0
22	2.930063772838016E-4	0.0	0.0	13.930109188826496	0.0
23	2.930063772838016E-4	0.0	0.0	14.365516665470224	0.0
24	2.930063772838016E-4	0.0	0.0	14.723130948945103	0.0
25	2.930063772838016E-4	0.0	0.0	15.067559945442213	0.0
26	2.930063772838016E-4	0.0	0.0	15.389280947699827	0.0
27	2.930063772838016E-4	0.0	0.0	15.769017212659634	0.0
28	2.930063772838016E-4	0.0	0.0	16.11007663581798	0.0
29	2.930063772838016E-4	0.0	0.0	16.487468849759516	0.0
30	2.930063772838016E-4	0.0	0.0	17.03348623382788	0.0
31	2.930063772838016E-4	0.0	0.0	17.410878447769417	0.0
32	2.930063772838016E-4	0.0	0.0	17.83427266294451	0.0
33	2.930063772838016E-4	0.0	0.0	18.217818010809005	0.0
34	2.930063772838016E-4	0.0	0.0	18.599312314032513	0.0
35	2.930063772838016E-4	0.0	0.0	19.01376983470045	0.0
36	2.930063772838016E-4	0.0	0.0	19.373142156439034	0.0
37	2.930063772838016E-4	0.0	0.0	19.74877633211687	0.0
38	2.930063772838016E-4	0.0	0.0	20.160157285823328	0.0
39	2.930063772838016E-4	0.0	0.0	20.791293022492635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	1360	0.0	40.036762	1
ACGTAGG	80	0.0	39.375	2
AGGGCGA	635	0.0	38.622047	6
TTAACCG	35	6.2445597E-6	38.57143	1
AATCGAT	35	6.2445597E-6	38.57143	33
CGTTAGG	90	0.0	37.5	2
CGATTTA	30	1.1394364E-4	37.499996	10
TCAATCG	30	1.1394364E-4	37.499996	31
ACGTATG	30	1.1394364E-4	37.499996	1
AATAGCG	30	1.1394364E-4	37.499996	1
GCGTATG	60	1.546141E-10	37.499996	1
TACGTAG	30	1.1394364E-4	37.499996	1
CACGACC	255	0.0	37.058823	27
CTATGCG	55	2.746674E-9	36.81818	1
TTAACGG	55	2.746674E-9	36.81818	2
GTAGGGA	525	0.0	36.42857	4
TACGGGA	235	0.0	36.38298	4
CCCTAGG	25	0.0021065103	36.0	2
ATAGACG	25	0.0021065103	36.0	1
TATGCGT	25	0.0021065103	36.0	13
>>END_MODULE