FastQCFastQC Report
Sat 14 Jan 2017
SRR2935736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861327
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC68160.791337088004904No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67400.7825134937137697No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG31790.3690816612041652No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC31320.36362496473464784No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT21810.2532139361705833No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC17930.20816716531584403No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG12480.14489270625441905No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC12330.14315120738116882No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT10520.12213712097728273No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT10060.1167965244326487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG207.0323E-445.0000041
TGCGACG406.8121153E-945.0000041
CGTTTTT41450.042.882991
ATCGCGG750.042.02
TACGCGG900.040.02
GCCGATA403.4568802E-739.3750049
CTACGGC403.4568802E-739.37500416
CGTAAGG1450.038.7931062
CTACGCG356.246466E-638.5714261
TTACGCG356.246466E-638.5714261
CGCATGG1650.038.1818162
GTCGATG301.1396841E-437.5000041
TAGTGCG552.748493E-936.818181
GCGCTAG800.036.5625041
ATTACGG250.002106815636.0000042
CTACGAA250.002106815636.00000411
AACTCGT250.002106815636.00000412
ACGTAAC250.002106815636.00000417
TATCGCG250.002106815636.0000041
AGCGACT2350.035.42553319
Produced by FastQC (version 0.11.5)