##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935736.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861327
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.095280886353265	33.0	31.0	34.0	30.0	34.0
2	32.1635221002012	33.0	31.0	34.0	30.0	34.0
3	32.15199686065803	33.0	31.0	34.0	30.0	34.0
4	35.819564462741795	37.0	35.0	37.0	35.0	37.0
5	34.950539110001195	37.0	35.0	37.0	33.0	37.0
6	35.377105327012856	37.0	35.0	37.0	33.0	37.0
7	35.91505665095835	37.0	35.0	37.0	35.0	37.0
8	35.88837108322391	37.0	35.0	37.0	35.0	37.0
9	37.73004793765899	39.0	38.0	39.0	35.0	39.0
10	37.35463070355394	39.0	37.0	39.0	34.0	39.0
11	37.32038819170884	39.0	37.0	39.0	34.0	39.0
12	37.29721929069912	39.0	37.0	39.0	34.0	39.0
13	37.2740956686601	39.0	37.0	39.0	34.0	39.0
14	38.41931229370495	40.0	38.0	41.0	34.0	41.0
15	38.48803648324039	40.0	38.0	41.0	34.0	41.0
16	38.49730590124308	40.0	38.0	41.0	34.0	41.0
17	38.46251307575404	40.0	38.0	41.0	34.0	41.0
18	38.38295444122848	40.0	38.0	41.0	34.0	41.0
19	38.24876150405131	40.0	37.0	41.0	34.0	41.0
20	38.1649152992998	40.0	37.0	41.0	34.0	41.0
21	38.097890812664644	40.0	36.0	41.0	34.0	41.0
22	38.14925806343003	40.0	37.0	41.0	34.0	41.0
23	38.135070652609286	40.0	37.0	41.0	34.0	41.0
24	38.095170591424626	40.0	36.0	41.0	34.0	41.0
25	37.933617545949446	40.0	36.0	41.0	34.0	41.0
26	37.94914474990335	40.0	36.0	41.0	34.0	41.0
27	37.96771144989069	40.0	36.0	41.0	34.0	41.0
28	37.833980590414555	40.0	36.0	41.0	33.0	41.0
29	37.7752723413988	40.0	36.0	41.0	33.0	41.0
30	37.62008273280647	40.0	36.0	41.0	33.0	41.0
31	37.510925583431145	40.0	36.0	41.0	33.0	41.0
32	37.33175553535417	40.0	36.0	41.0	32.0	41.0
33	37.08180284607356	40.0	35.0	41.0	32.0	41.0
34	36.9179870130624	40.0	35.0	41.0	31.0	41.0
35	36.788160594060095	40.0	35.0	41.0	31.0	41.0
36	36.71972781533611	40.0	35.0	41.0	31.0	41.0
37	36.67351656223478	40.0	35.0	41.0	31.0	41.0
38	36.56740239189065	40.0	35.0	41.0	30.0	41.0
39	36.47764089596634	40.0	35.0	41.0	30.0	41.0
40	36.365725212375786	39.0	35.0	41.0	30.0	41.0
41	36.26828370642044	39.0	35.0	41.0	29.0	41.0
42	36.285511774273886	39.0	35.0	41.0	29.0	41.0
43	36.18496227332941	39.0	35.0	41.0	29.0	41.0
44	36.07585504692179	39.0	35.0	41.0	29.0	41.0
45	36.01153104453942	39.0	35.0	41.0	29.0	41.0
46	35.87607610117876	39.0	35.0	41.0	28.0	41.0
47	35.73007464064171	39.0	35.0	41.0	28.0	41.0
48	35.6468797564688	39.0	35.0	41.0	28.0	41.0
49	35.57742181540809	38.0	35.0	40.0	28.0	41.0
50	35.43280194397714	38.0	35.0	40.0	27.0	41.0
51	34.308508847394776	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	15.0
11	13.0
12	12.0
13	21.0
14	26.0
15	33.0
16	56.0
17	136.0
18	262.0
19	477.0
20	926.0
21	1607.0
22	2362.0
23	3323.0
24	5081.0
25	7866.0
26	10292.0
27	11268.0
28	11167.0
29	11721.0
30	13239.0
31	15727.0
32	20507.0
33	27973.0
34	47257.0
35	60088.0
36	60731.0
37	91188.0
38	164487.0
39	293206.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05528678422945	18.115883979023064	20.793844846382385	21.0349843903651
2	32.47233629039842	26.512462746436604	23.949556904636683	17.065644058528296
3	29.698244685235686	25.8828528537942	28.18383726505729	16.235065195912817
4	26.425155602924328	24.69027442539245	30.471237985109024	18.413331986574207
5	24.59356318796462	29.95099422170674	27.287313645108068	18.16812894522057
6	24.647549653035377	33.96213052650155	27.4886309148558	13.90168890560728
7	76.65880670175207	5.872682500374422	13.165499281921964	4.30301151595155
8	78.23555978159283	5.992845922628688	11.228836434942826	4.542757860835664
9	71.95583094457739	6.790220206727526	13.602731599032655	7.65121724966244
10	36.60491311662121	25.2009979949543	22.177872050916783	16.016216837507706
11	28.29564149271995	25.816791996535578	27.58719975108176	18.300366759662705
12	24.084232817501366	22.717388401849703	32.827602060541466	20.37077672010746
13	23.360117586003923	23.932606315603714	34.774481700910336	17.932794397482024
14	21.114164539135544	26.940058769781977	32.49892317319671	19.446853517885774
15	19.811291182094607	25.827124889850197	35.75842856429672	18.603155363758482
16	23.099241054791037	25.74330074408442	31.197094715479718	19.960363485644823
17	23.594291134493638	25.765127529962488	29.866241276541892	20.77434005900198
18	24.157956269802295	24.825763037731313	30.993107147459675	20.023173545006717
19	23.397269561966596	26.968503251378394	28.859887127653028	20.77434005900198
20	23.835662878326115	26.666411246831924	29.98733349819523	19.51059237664673
21	23.28767123287671	28.079695632436923	30.352351662028475	18.280281472657887
22	22.359684533284106	24.895190792811555	31.16481893636215	21.580305737542187
23	21.13390152636571	26.935995272411056	30.86876412790961	21.06133907331362
24	21.482085201090875	25.91118123546574	30.839274746989236	21.76745881645415
25	22.00848226051198	28.132056698559317	28.900638201287084	20.958822839641623
26	21.60271302304467	25.65901219861911	29.99267409473986	22.745600683596358
27	22.404266904439314	25.606070632872303	29.44027065214489	22.549391810543497
28	21.38618666313723	26.09310981775795	30.49457407001058	22.02612944909425
29	23.111663746753557	25.935562219691242	28.62803557766098	22.32473845589422
30	22.97826493306259	25.870546261756566	29.166274829420185	21.98491397576066
31	24.244102414065736	25.559746762843844	28.30759978498294	21.88855103810748
32	24.315039468169463	26.778215474494587	27.972535401769594	20.934209655566352
33	22.88991289022636	27.769708832998386	27.021560917050085	22.31881735972517
34	22.613130669304457	26.873881812598466	29.680133096953888	20.83285442114319
35	21.19032608985902	27.735575455082678	28.44657139506831	22.62752705998999
36	22.74153718622544	29.209580101401674	26.78518146998759	21.263701242385295
37	21.19961408384969	28.630241476233763	28.595527598693643	21.574616841222905
38	21.42368693887455	28.927108984160487	27.37369198922128	22.275512087743678
39	21.680267772866753	28.327104572363343	28.058101046408623	21.934526608361285
40	22.353763437115056	26.857976122889447	29.415076968445202	21.373183471550295
41	20.477356451150374	27.282205248413206	28.975870952611494	23.264567347824926
42	21.774424811947146	26.75882678703907	29.044602108142435	22.422146292871346
43	22.132941379986928	26.6114959823621	28.60527999238385	22.65028264526713
44	22.50678313811131	26.194929451880643	28.25396161968683	23.044325790321214
45	21.468617609804404	25.73772794769002	28.25326502013753	24.540389422368044
46	23.118629742246558	26.59837669085028	27.490488513653933	22.792505053249233
47	20.895083980880663	26.077436327898695	30.345966166159887	22.681513525060748
48	21.278097633070832	26.749887092823048	28.677726345511058	23.294288928595066
49	21.793000799928482	25.45630172977278	29.902348353180614	22.848349117118122
50	20.699571707377103	25.80088630682656	30.4030873292025	23.09645465659384
51	20.652667337724232	24.647665752960258	29.11646796164523	25.583198947670283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	630.5
2	1000.0
3	6181.0
4	11362.0
5	7506.5
6	3651.0
7	3609.0
8	3567.0
9	3633.0
10	3699.0
11	3625.0
12	3551.0
13	3520.0
14	3489.0
15	3422.5
16	3356.0
17	3302.5
18	3249.0
19	3175.5
20	3102.0
21	3490.5
22	3879.0
23	4020.0
24	4161.0
25	4923.0
26	6543.0
27	7401.0
28	9336.5
29	11272.0
30	12734.0
31	14196.0
32	16931.5
33	19667.0
34	21328.0
35	22989.0
36	25807.5
37	28626.0
38	30840.5
39	33055.0
40	35876.5
41	38698.0
42	42523.0
43	46348.0
44	51324.0
45	56300.0
46	71780.0
47	87260.0
48	83851.5
49	80443.0
50	79821.0
51	79199.0
52	70600.0
53	62001.0
54	56561.0
55	51121.0
56	46348.0
57	41575.0
58	38234.5
59	34894.0
60	31312.0
61	27730.0
62	24654.5
63	21579.0
64	18730.0
65	15881.0
66	12895.5
67	9910.0
68	8072.0
69	6234.0
70	5244.5
71	4255.0
72	3588.5
73	2922.0
74	2307.5
75	1361.0
76	1029.0
77	786.5
78	544.0
79	389.0
80	234.0
81	187.5
82	141.0
83	116.5
84	92.0
85	50.0
86	8.0
87	7.0
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	861327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.39699230843506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82214282827829	26.86903964713039
2	8.752737763727266	6.371466581282608
3	3.4807591178194617	3.8006748851637053
4	2.1832107237188993	3.1784921567555893
5	1.5503998957956155	2.8214946541135766
6	1.1772769175025832	2.5709603346743797
7	1.0127385410135006	2.5802445821406885
8	0.8910977106999569	2.5946621217928336
9	0.7207975014442627	2.361137500440544
>10	6.325197666469664	40.27894889354744
>50	0.05459036846936847	1.3083491811721004
>100	0.023623861698130272	1.410830686085097
>500	0.0022346896200832497	0.5561057209342456
>1k	0.002553930994380857	1.7224636872397108
>5k	6.384827485952142E-4	1.5751293675270859
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	6816	0.791337088004904	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6740	0.7825134937137697	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	3179	0.3690816612041652	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	3132	0.36362496473464784	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	2181	0.2532139361705833	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1793	0.20816716531584403	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	1248	0.14489270625441905	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1233	0.14315120738116882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	1052	0.12213712097728273	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	1006	0.1167965244326487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.643996995333944E-4	0.0	0.0	0.05955926146515783	0.0
2	4.643996995333944E-4	0.0	0.0	0.49237978143028144	0.0
3	4.643996995333944E-4	0.0	0.0	0.851244649244712	0.0
4	4.643996995333944E-4	0.0	0.0	1.436736570431439	0.0
5	4.643996995333944E-4	0.0	0.0	2.982839270102992	0.0
6	4.643996995333944E-4	0.0	0.0	4.236718458843157	0.0
7	5.80499624416743E-4	0.0	0.0	5.075888715900001	0.0
8	6.965995493000916E-4	0.0	0.0	6.269163743851058	0.0
9	6.965995493000916E-4	0.0	0.0	6.870329154897036	0.0
10	6.965995493000916E-4	0.0	0.0	8.10714165467935	0.0
11	6.965995493000916E-4	0.0	0.0	8.925878324956724	0.0
12	8.126994741834402E-4	0.0	0.0	10.05947799151774	0.0
13	8.126994741834402E-4	0.0	0.0	10.496245908928897	0.0
14	8.126994741834402E-4	0.0	0.0	10.731232156892794	0.0
15	8.126994741834402E-4	0.0	0.0	10.993850186978928	0.0
16	8.126994741834402E-4	0.0	0.0	11.397297425948565	0.0
17	8.126994741834402E-4	0.0	0.0	11.859142927134526	0.0
18	8.126994741834402E-4	0.0	0.0	12.411198069954848	0.0
19	8.126994741834402E-4	0.0	0.0	12.787477926501781	0.0
20	9.287993990667888E-4	0.0	0.0	13.141814897245762	0.0
21	9.287993990667888E-4	0.0	0.0	13.526337848459413	0.0
22	9.287993990667888E-4	0.0	0.0	13.944878077663883	0.0
23	9.287993990667888E-4	0.0	0.0	14.361328508220454	0.0
24	9.287993990667888E-4	0.0	0.0	14.700108089030067	0.0
25	9.287993990667888E-4	0.0	0.0	15.015203285163475	0.0
26	9.287993990667888E-4	0.0	0.0	15.31671479008553	0.0
27	9.287993990667888E-4	0.0	0.0	15.659093468566526	0.0
28	0.0010448993239501375	0.0	0.0	15.980690260493402	0.0
29	0.0010448993239501375	0.0	0.0	16.351165120796168	0.0
30	0.0010448993239501375	0.0	0.0	16.86374628915615	0.0
31	0.001160999248833486	0.0	0.0	17.253261537139785	0.0
32	0.001160999248833486	0.0	0.0	17.63058629301067	0.0
33	0.001160999248833486	0.0	0.0	18.019172741595238	0.0
34	0.001160999248833486	0.0	0.0	18.39998049521262	0.0
35	0.001160999248833486	0.0	0.0	18.807839531327822	0.0
36	0.001160999248833486	0.0	0.0	19.16554339989342	0.0
37	0.0012770991737168346	0.0	0.0	19.536830959670368	0.0
38	0.0013931990986001833	0.0	0.0	19.946895894358356	0.0
39	0.0013931990986001833	0.0	0.0	20.470042155882727	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAG	20	7.0323E-4	45.000004	1
TGCGACG	40	6.8121153E-9	45.000004	1
CGTTTTT	4145	0.0	42.88299	1
ATCGCGG	75	0.0	42.0	2
TACGCGG	90	0.0	40.0	2
GCCGATA	40	3.4568802E-7	39.375004	9
CTACGGC	40	3.4568802E-7	39.375004	16
CGTAAGG	145	0.0	38.793106	2
CTACGCG	35	6.246466E-6	38.571426	1
TTACGCG	35	6.246466E-6	38.571426	1
CGCATGG	165	0.0	38.181816	2
GTCGATG	30	1.1396841E-4	37.500004	1
TAGTGCG	55	2.748493E-9	36.81818	1
GCGCTAG	80	0.0	36.562504	1
ATTACGG	25	0.0021068156	36.000004	2
CTACGAA	25	0.0021068156	36.000004	11
AACTCGT	25	0.0021068156	36.000004	12
ACGTAAC	25	0.0021068156	36.000004	17
TATCGCG	25	0.0021068156	36.000004	1
AGCGACT	235	0.0	35.425533	19
>>END_MODULE