FastQCFastQC Report
Sat 14 Jan 2017
SRR2935735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153320
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC194121.6831408455589083No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101960.884056463080498No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC81660.7080428675476017No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG81050.7027537890611452No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT59230.5135608504144556No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC38110.3304373460964867No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT34200.29653522006034755No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT20040.17375923421080014No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC19340.16768979988207958No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG15620.13543509173516458No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC15500.13439461727881247No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT13080.11341171574237852No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGT11790.1022266153365935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG302.1655087E-645.0000041
GCACGAA207.033297E-445.022
CATACCG207.033297E-445.01
CGTTTTT47000.043.180851
CTATGCG603.6379788E-1241.2500041
TTAACGG1200.041.2500042
TTACGCG356.248307E-638.571431
AATCGTT601.5643309E-1037.50000422
CGTATCG250.002107111736.013
CGTTACC250.002107111736.023
ATATACG250.002107111736.01
TCGTATC250.002107111736.012
TCATCGA250.002107111736.016
TATAGCG504.8790753E-836.01
GTACGAA250.002107111736.012
TACGGCT11450.035.96077
GAATCTG27850.035.3052061
ACGGCTG11950.035.2092068
CGATGAA7000.035.03571319
GTATACG458.6999535E-735.0000041
Produced by FastQC (version 0.11.5)