##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935735.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1153320
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13797384940867	33.0	31.0	34.0	30.0	34.0
2	32.204878091076196	33.0	31.0	34.0	30.0	34.0
3	32.20880154683869	34.0	31.0	34.0	30.0	34.0
4	35.85804633579579	37.0	35.0	37.0	35.0	37.0
5	34.909456178684145	37.0	35.0	37.0	33.0	37.0
6	35.354119411785106	37.0	35.0	37.0	33.0	37.0
7	35.87789165192661	37.0	35.0	37.0	35.0	37.0
8	35.82869368432005	37.0	35.0	37.0	35.0	37.0
9	37.697147365865504	39.0	38.0	39.0	35.0	39.0
10	37.43121336662852	39.0	37.0	39.0	35.0	39.0
11	37.35374223979468	39.0	37.0	39.0	34.0	39.0
12	37.254285887698124	39.0	37.0	39.0	34.0	39.0
13	37.169995317864945	39.0	37.0	39.0	34.0	39.0
14	38.278503797731766	40.0	38.0	41.0	33.0	41.0
15	38.34250684979017	40.0	38.0	41.0	33.0	41.0
16	38.40600613879929	40.0	38.0	41.0	34.0	41.0
17	38.35873825130926	40.0	37.0	41.0	34.0	41.0
18	38.28599781500364	40.0	37.0	41.0	34.0	41.0
19	38.18190007976971	40.0	37.0	41.0	34.0	41.0
20	38.05807928415357	40.0	36.0	41.0	34.0	41.0
21	38.06640307980439	40.0	36.0	41.0	34.0	41.0
22	38.11471577706101	40.0	36.0	41.0	34.0	41.0
23	38.11452589047272	40.0	36.0	41.0	34.0	41.0
24	38.067808587382515	40.0	36.0	41.0	34.0	41.0
25	37.92036642042105	40.0	36.0	41.0	34.0	41.0
26	37.89452970554573	40.0	36.0	41.0	34.0	41.0
27	37.90714025595672	40.0	36.0	41.0	34.0	41.0
28	37.767044705719144	40.0	36.0	41.0	33.0	41.0
29	37.732431588804495	40.0	36.0	41.0	33.0	41.0
30	37.59404154961329	40.0	35.0	41.0	33.0	41.0
31	37.535022370200814	40.0	35.0	41.0	33.0	41.0
32	37.38145874518781	40.0	35.0	41.0	33.0	41.0
33	37.180387056497764	40.0	35.0	41.0	32.0	41.0
34	37.0038792355981	40.0	35.0	41.0	32.0	41.0
35	36.86842160024971	40.0	35.0	41.0	31.0	41.0
36	36.7956499497104	40.0	35.0	41.0	31.0	41.0
37	36.744682308466	39.0	35.0	41.0	31.0	41.0
38	36.628300905212775	39.0	35.0	41.0	31.0	41.0
39	36.52929022300836	39.0	35.0	41.0	31.0	41.0
40	36.354815662608814	39.0	35.0	41.0	30.0	41.0
41	36.265371275968505	39.0	35.0	41.0	30.0	41.0
42	36.26933981895745	39.0	35.0	41.0	30.0	41.0
43	36.20551451461866	39.0	35.0	41.0	30.0	41.0
44	36.14089584850692	39.0	35.0	41.0	30.0	41.0
45	36.12581763951028	39.0	35.0	41.0	30.0	41.0
46	35.983288246106895	39.0	35.0	41.0	30.0	41.0
47	35.844064093226514	38.0	35.0	41.0	29.0	41.0
48	35.80908074081781	38.0	35.0	40.0	29.0	41.0
49	35.78488797558353	38.0	35.0	40.0	29.0	41.0
50	35.65368414663753	38.0	35.0	40.0	29.0	41.0
51	34.54231522907779	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	16.0
10	21.0
11	17.0
12	18.0
13	22.0
14	29.0
15	39.0
16	92.0
17	146.0
18	307.0
19	677.0
20	1246.0
21	2254.0
22	3359.0
23	4531.0
24	6127.0
25	8605.0
26	11327.0
27	12789.0
28	13696.0
29	14562.0
30	16808.0
31	21383.0
32	28593.0
33	40507.0
34	73155.0
35	87055.0
36	85144.0
37	123435.0
38	210743.0
39	386307.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70046474525717	16.332934484791732	20.266101342212046	20.70049942773905
2	29.696788402178058	28.793483161655047	24.76086428744841	16.748864148718482
3	29.42791246141574	28.050931224638436	27.185863420386365	15.335292893559464
4	26.443831720597927	23.731488225297404	32.11485103874033	17.70982901536434
5	26.68747615579371	28.47717892692401	26.10030173759234	18.73504317968994
6	24.723580619429125	34.1442097596504	26.55178094544446	14.580428675476018
7	75.43509173516458	5.371796205736482	14.673204314500746	4.519907744598203
8	76.13628481254119	7.18308882183609	11.58386224118198	5.096764124440744
9	70.11792043838658	6.3507092567544134	13.360645787812576	10.17072451704644
10	39.988381368570735	24.49190164048139	20.032428120556308	15.487288870391566
11	29.69488086567475	23.891461172961538	27.35970936080186	19.053948600561856
12	25.19309471785801	20.78104949190164	32.35337980785905	21.672475982381297
13	24.73979467970728	21.469670169597336	35.40483126972566	18.38570388096972
14	20.113238303332988	26.24440744979711	33.05422606041689	20.588128186453023
15	18.737644365830818	23.98718482294593	36.96953143966982	20.305639371553426
16	22.838934554156694	24.49996531751812	30.50844518433739	22.15265494398779
17	23.222956334755317	24.790951340477925	29.68924496236951	22.296847362397255
18	23.974439010855615	23.56735337980786	30.248153157839972	22.21005445149655
19	23.253303506398918	25.964259702424307	28.065324454617972	22.717112336558802
20	24.42114937744945	25.94214962022682	29.52450317344709	20.112197828876635
21	23.665591509728436	27.040110290292375	30.055145146186664	19.239153053792528
22	22.314362015745846	22.324766760309366	31.34559359067735	24.015277633267438
23	21.99060104741095	25.198470502549164	31.084347795928274	21.726580654111608
24	23.43113793223043	24.05386189435716	30.01170533763396	22.503294835778448
25	22.310980473762704	27.899368778829814	27.499306350362435	22.290344397045054
26	21.24310685672667	23.853917386328167	29.643724204904103	25.25925155204106
27	22.93855998335241	23.786199840460583	29.395397634654735	23.879842541532273
28	20.326275448271076	24.575486421808346	31.036312558526692	24.06192557139389
29	21.745395900530642	23.2992577948878	31.76629209586238	23.189054208719178
30	22.347223667325633	23.501456664238894	33.273939583116565	20.877380085318904
31	22.90465785731627	22.586619498491313	31.591579093399925	22.917143550792495
32	24.313373565012313	23.93464086290015	29.030451219089237	22.7215343529983
33	25.4691672736101	22.512919224499704	28.16763777615926	23.85027572573093
34	22.78517670724517	23.276800887871534	30.3714493809177	23.566573023965596
35	22.02129504387334	26.177730378385878	28.972965005375784	22.828009572364998
36	24.765546422501995	25.832813096105156	28.76955224915895	20.6320882322339
37	22.33647209794333	24.98742760031908	29.874969652828355	22.801130648909236
38	23.297523670793883	24.874362709395484	28.226164464329067	23.601949155481567
39	24.57097769916415	23.204054382131588	26.97022508930739	25.25474282939687
40	24.849131203828946	23.991866957999513	29.264211146949677	21.894790691221864
41	22.095255436479032	25.11427877778934	27.825581798633507	24.964883987098116
42	21.900600006936497	25.08523219921618	30.558648076856382	22.45551971699095
43	23.941230534457045	23.168244719592135	29.326813026740194	23.563711719210627
44	23.113879929247737	22.676880657579858	29.114469531439667	25.094769881732738
45	21.739933409634794	21.99918496167586	28.85773245933479	27.403149169354556
46	24.345888391773315	23.830420004855547	28.34538549578608	23.47830610758506
47	20.721395623070787	23.614521555162487	32.38814899594215	23.275933825824577
48	21.246921929733293	24.371986959386813	29.69279991676204	24.688291194117852
49	22.13834842021295	22.252453785592895	31.99346235216592	23.61573544202823
50	21.235996947941594	22.71243020150522	31.577706100648562	24.473866749904623
51	20.585266881698054	22.189938612007076	29.99878611313426	27.226008393160615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	368.0
1	978.5
2	1589.0
3	8783.0
4	15977.0
5	10197.0
6	4417.0
7	4246.0
8	4075.0
9	4114.5
10	4154.0
11	4064.5
12	3975.0
13	3837.5
14	3700.0
15	3496.0
16	3292.0
17	3128.5
18	2965.0
19	3150.0
20	3335.0
21	3593.0
22	3851.0
23	4613.5
24	5376.0
25	6054.5
26	8048.0
27	9363.0
28	11204.5
29	13046.0
30	15181.0
31	17316.0
32	19625.0
33	21934.0
34	25627.0
35	29320.0
36	30721.0
37	32122.0
38	35630.0
39	39138.0
40	41813.5
41	44489.0
42	49184.5
43	53880.0
44	59430.0
45	64980.0
46	98116.0
47	131252.0
48	115016.5
49	98781.0
50	98927.0
51	99073.0
52	88291.5
53	77510.0
54	71491.0
55	65472.0
56	61859.0
57	58246.0
58	55489.5
59	52733.0
60	49633.5
61	46534.0
62	42647.0
63	38760.0
64	35026.5
65	31293.0
66	26311.5
67	21330.0
68	18230.5
69	15131.0
70	12955.5
71	10780.0
72	8986.0
73	7192.0
74	5900.0
75	3535.0
76	2462.0
77	1940.0
78	1418.0
79	1070.5
80	723.0
81	533.0
82	343.0
83	274.0
84	205.0
85	124.0
86	43.0
87	28.0
88	13.0
89	11.5
90	10.0
91	7.0
92	4.0
93	5.5
94	7.0
95	3.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1153320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.353300143522784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92714772023375	23.17860051227035
2	8.79456062643276	5.514769979019083
3	3.319540647889881	3.1223566281574655
4	1.8618378232770632	2.3349904036707554
5	1.3008347846809054	2.0392731720617627
6	1.0283780557957005	1.9345827506625013
7	0.8658316941408393	1.9002676686120787
8	0.724326025903309	1.8168009033529207
9	0.6757115523078427	1.9067208398958142
>10	7.365499916484914	45.749164928024776
>50	0.09686768852136585	1.9759364833530118
>100	0.03311619385130739	1.8820953616916005
>500	0.0022077462540222316	0.5331680585892057
>1k	0.0027596828175277897	1.629095781745791
>5k	8.279048452583369E-4	1.920339482688872
>10k+	5.519365635055579E-4	2.5618370462040247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	19412	1.6831408455589083	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10196	0.884056463080498	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	8166	0.7080428675476017	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	8105	0.7027537890611452	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	5923	0.5135608504144556	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	3811	0.3304373460964867	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	3420	0.29653522006034755	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	2004	0.17375923421080014	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC	1934	0.16768979988207958	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	1562	0.13543509173516458	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	1550	0.13439461727881247	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	1308	0.11341171574237852	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGT	1179	0.1022266153365935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.734124093920161E-4	0.0	0.0	0.09650400582665696	0.0
2	1.734124093920161E-4	0.0	0.0	0.6394582596330594	0.0
3	1.734124093920161E-4	0.0	0.0	0.9765719834911386	0.0
4	1.734124093920161E-4	0.0	0.0	1.81103249748552	0.0
5	1.734124093920161E-4	0.0	0.0	4.245569312940034	0.0
6	1.734124093920161E-4	0.0	0.0	5.664603058994902	0.0
7	1.734124093920161E-4	0.0	0.0	6.5905386189435715	0.0
8	1.734124093920161E-4	0.0	0.0	7.843356570596192	0.0
9	1.734124093920161E-4	0.0	0.0	8.447698817327367	0.0
10	1.734124093920161E-4	0.0	0.0	10.011185100405784	0.0
11	2.6011861408802413E-4	0.0	0.0	10.825876599729476	0.0
12	2.6011861408802413E-4	0.0	0.0	12.309593174487567	0.0
13	2.6011861408802413E-4	0.0	0.0	12.734713696112093	0.0
14	2.6011861408802413E-4	0.0	0.0	12.98460097804599	0.0
15	2.6011861408802413E-4	0.0	0.0	13.287639163458538	0.0
16	2.6011861408802413E-4	0.0	0.0	13.677990496999966	0.0
17	2.6011861408802413E-4	0.0	0.0	14.08533624666181	0.0
18	2.6011861408802413E-4	0.0	0.0	14.591093538653626	0.0
19	2.6011861408802413E-4	0.0	0.0	14.998525994520168	0.0
20	2.6011861408802413E-4	0.0	0.0	15.33035063989179	0.0
21	2.6011861408802413E-4	0.0	0.0	15.676828633857038	0.0
22	2.6011861408802413E-4	0.0	0.0	16.103856691984877	0.0
23	3.468248187840322E-4	0.0	0.0	16.462040023584088	0.0
24	3.468248187840322E-4	0.0	0.0	16.776350015607118	0.0
25	3.468248187840322E-4	0.0	0.0	17.046006312211702	0.0
26	3.468248187840322E-4	0.0	0.0	17.336992335171505	0.0
27	3.468248187840322E-4	0.0	0.0	17.673672528006104	0.0
28	3.468248187840322E-4	0.0	0.0	17.96890715499601	0.0
29	3.468248187840322E-4	0.0	0.0	18.2970901397704	0.0
30	3.468248187840322E-4	0.0	0.0	18.796431172614714	0.0
31	3.468248187840322E-4	0.0	0.0	19.145683765130233	0.0
32	3.468248187840322E-4	0.0	0.0	19.501786147816738	0.0
33	3.468248187840322E-4	0.0	0.0	19.8458363680505	0.0
34	3.468248187840322E-4	0.0	0.0	20.179481843720737	0.0
35	3.468248187840322E-4	0.0	0.0	20.54451496549093	0.0
36	3.468248187840322E-4	0.0	0.0	20.880674921097352	0.0
37	3.468248187840322E-4	0.0	0.0	21.237644365830818	0.0
38	3.468248187840322E-4	0.0	0.0	21.64906530711338	0.0
39	3.468248187840322E-4	0.0	0.0	22.231557590261158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	30	2.1655087E-6	45.000004	1
GCACGAA	20	7.033297E-4	45.0	22
CATACCG	20	7.033297E-4	45.0	1
CGTTTTT	4700	0.0	43.18085	1
CTATGCG	60	3.6379788E-12	41.250004	1
TTAACGG	120	0.0	41.250004	2
TTACGCG	35	6.248307E-6	38.57143	1
AATCGTT	60	1.5643309E-10	37.500004	22
CGTATCG	25	0.0021071117	36.0	13
CGTTACC	25	0.0021071117	36.0	23
ATATACG	25	0.0021071117	36.0	1
TCGTATC	25	0.0021071117	36.0	12
TCATCGA	25	0.0021071117	36.0	16
TATAGCG	50	4.8790753E-8	36.0	1
GTACGAA	25	0.0021071117	36.0	12
TACGGCT	1145	0.0	35.9607	7
GAATCTG	2785	0.0	35.305206	1
ACGGCTG	1195	0.0	35.209206	8
CGATGAA	700	0.0	35.035713	19
GTATACG	45	8.6999535E-7	35.000004	1
>>END_MODULE