##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935735.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1153320 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13797384940867 33.0 31.0 34.0 30.0 34.0 2 32.204878091076196 33.0 31.0 34.0 30.0 34.0 3 32.20880154683869 34.0 31.0 34.0 30.0 34.0 4 35.85804633579579 37.0 35.0 37.0 35.0 37.0 5 34.909456178684145 37.0 35.0 37.0 33.0 37.0 6 35.354119411785106 37.0 35.0 37.0 33.0 37.0 7 35.87789165192661 37.0 35.0 37.0 35.0 37.0 8 35.82869368432005 37.0 35.0 37.0 35.0 37.0 9 37.697147365865504 39.0 38.0 39.0 35.0 39.0 10 37.43121336662852 39.0 37.0 39.0 35.0 39.0 11 37.35374223979468 39.0 37.0 39.0 34.0 39.0 12 37.254285887698124 39.0 37.0 39.0 34.0 39.0 13 37.169995317864945 39.0 37.0 39.0 34.0 39.0 14 38.278503797731766 40.0 38.0 41.0 33.0 41.0 15 38.34250684979017 40.0 38.0 41.0 33.0 41.0 16 38.40600613879929 40.0 38.0 41.0 34.0 41.0 17 38.35873825130926 40.0 37.0 41.0 34.0 41.0 18 38.28599781500364 40.0 37.0 41.0 34.0 41.0 19 38.18190007976971 40.0 37.0 41.0 34.0 41.0 20 38.05807928415357 40.0 36.0 41.0 34.0 41.0 21 38.06640307980439 40.0 36.0 41.0 34.0 41.0 22 38.11471577706101 40.0 36.0 41.0 34.0 41.0 23 38.11452589047272 40.0 36.0 41.0 34.0 41.0 24 38.067808587382515 40.0 36.0 41.0 34.0 41.0 25 37.92036642042105 40.0 36.0 41.0 34.0 41.0 26 37.89452970554573 40.0 36.0 41.0 34.0 41.0 27 37.90714025595672 40.0 36.0 41.0 34.0 41.0 28 37.767044705719144 40.0 36.0 41.0 33.0 41.0 29 37.732431588804495 40.0 36.0 41.0 33.0 41.0 30 37.59404154961329 40.0 35.0 41.0 33.0 41.0 31 37.535022370200814 40.0 35.0 41.0 33.0 41.0 32 37.38145874518781 40.0 35.0 41.0 33.0 41.0 33 37.180387056497764 40.0 35.0 41.0 32.0 41.0 34 37.0038792355981 40.0 35.0 41.0 32.0 41.0 35 36.86842160024971 40.0 35.0 41.0 31.0 41.0 36 36.7956499497104 40.0 35.0 41.0 31.0 41.0 37 36.744682308466 39.0 35.0 41.0 31.0 41.0 38 36.628300905212775 39.0 35.0 41.0 31.0 41.0 39 36.52929022300836 39.0 35.0 41.0 31.0 41.0 40 36.354815662608814 39.0 35.0 41.0 30.0 41.0 41 36.265371275968505 39.0 35.0 41.0 30.0 41.0 42 36.26933981895745 39.0 35.0 41.0 30.0 41.0 43 36.20551451461866 39.0 35.0 41.0 30.0 41.0 44 36.14089584850692 39.0 35.0 41.0 30.0 41.0 45 36.12581763951028 39.0 35.0 41.0 30.0 41.0 46 35.983288246106895 39.0 35.0 41.0 30.0 41.0 47 35.844064093226514 38.0 35.0 41.0 29.0 41.0 48 35.80908074081781 38.0 35.0 40.0 29.0 41.0 49 35.78488797558353 38.0 35.0 40.0 29.0 41.0 50 35.65368414663753 38.0 35.0 40.0 29.0 41.0 51 34.54231522907779 37.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 12.0 9 16.0 10 21.0 11 17.0 12 18.0 13 22.0 14 29.0 15 39.0 16 92.0 17 146.0 18 307.0 19 677.0 20 1246.0 21 2254.0 22 3359.0 23 4531.0 24 6127.0 25 8605.0 26 11327.0 27 12789.0 28 13696.0 29 14562.0 30 16808.0 31 21383.0 32 28593.0 33 40507.0 34 73155.0 35 87055.0 36 85144.0 37 123435.0 38 210743.0 39 386307.0 40 297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70046474525717 16.332934484791732 20.266101342212046 20.70049942773905 2 29.696788402178058 28.793483161655047 24.76086428744841 16.748864148718482 3 29.42791246141574 28.050931224638436 27.185863420386365 15.335292893559464 4 26.443831720597927 23.731488225297404 32.11485103874033 17.70982901536434 5 26.68747615579371 28.47717892692401 26.10030173759234 18.73504317968994 6 24.723580619429125 34.1442097596504 26.55178094544446 14.580428675476018 7 75.43509173516458 5.371796205736482 14.673204314500746 4.519907744598203 8 76.13628481254119 7.18308882183609 11.58386224118198 5.096764124440744 9 70.11792043838658 6.3507092567544134 13.360645787812576 10.17072451704644 10 39.988381368570735 24.49190164048139 20.032428120556308 15.487288870391566 11 29.69488086567475 23.891461172961538 27.35970936080186 19.053948600561856 12 25.19309471785801 20.78104949190164 32.35337980785905 21.672475982381297 13 24.73979467970728 21.469670169597336 35.40483126972566 18.38570388096972 14 20.113238303332988 26.24440744979711 33.05422606041689 20.588128186453023 15 18.737644365830818 23.98718482294593 36.96953143966982 20.305639371553426 16 22.838934554156694 24.49996531751812 30.50844518433739 22.15265494398779 17 23.222956334755317 24.790951340477925 29.68924496236951 22.296847362397255 18 23.974439010855615 23.56735337980786 30.248153157839972 22.21005445149655 19 23.253303506398918 25.964259702424307 28.065324454617972 22.717112336558802 20 24.42114937744945 25.94214962022682 29.52450317344709 20.112197828876635 21 23.665591509728436 27.040110290292375 30.055145146186664 19.239153053792528 22 22.314362015745846 22.324766760309366 31.34559359067735 24.015277633267438 23 21.99060104741095 25.198470502549164 31.084347795928274 21.726580654111608 24 23.43113793223043 24.05386189435716 30.01170533763396 22.503294835778448 25 22.310980473762704 27.899368778829814 27.499306350362435 22.290344397045054 26 21.24310685672667 23.853917386328167 29.643724204904103 25.25925155204106 27 22.93855998335241 23.786199840460583 29.395397634654735 23.879842541532273 28 20.326275448271076 24.575486421808346 31.036312558526692 24.06192557139389 29 21.745395900530642 23.2992577948878 31.76629209586238 23.189054208719178 30 22.347223667325633 23.501456664238894 33.273939583116565 20.877380085318904 31 22.90465785731627 22.586619498491313 31.591579093399925 22.917143550792495 32 24.313373565012313 23.93464086290015 29.030451219089237 22.7215343529983 33 25.4691672736101 22.512919224499704 28.16763777615926 23.85027572573093 34 22.78517670724517 23.276800887871534 30.3714493809177 23.566573023965596 35 22.02129504387334 26.177730378385878 28.972965005375784 22.828009572364998 36 24.765546422501995 25.832813096105156 28.76955224915895 20.6320882322339 37 22.33647209794333 24.98742760031908 29.874969652828355 22.801130648909236 38 23.297523670793883 24.874362709395484 28.226164464329067 23.601949155481567 39 24.57097769916415 23.204054382131588 26.97022508930739 25.25474282939687 40 24.849131203828946 23.991866957999513 29.264211146949677 21.894790691221864 41 22.095255436479032 25.11427877778934 27.825581798633507 24.964883987098116 42 21.900600006936497 25.08523219921618 30.558648076856382 22.45551971699095 43 23.941230534457045 23.168244719592135 29.326813026740194 23.563711719210627 44 23.113879929247737 22.676880657579858 29.114469531439667 25.094769881732738 45 21.739933409634794 21.99918496167586 28.85773245933479 27.403149169354556 46 24.345888391773315 23.830420004855547 28.34538549578608 23.47830610758506 47 20.721395623070787 23.614521555162487 32.38814899594215 23.275933825824577 48 21.246921929733293 24.371986959386813 29.69279991676204 24.688291194117852 49 22.13834842021295 22.252453785592895 31.99346235216592 23.61573544202823 50 21.235996947941594 22.71243020150522 31.577706100648562 24.473866749904623 51 20.585266881698054 22.189938612007076 29.99878611313426 27.226008393160615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 368.0 1 978.5 2 1589.0 3 8783.0 4 15977.0 5 10197.0 6 4417.0 7 4246.0 8 4075.0 9 4114.5 10 4154.0 11 4064.5 12 3975.0 13 3837.5 14 3700.0 15 3496.0 16 3292.0 17 3128.5 18 2965.0 19 3150.0 20 3335.0 21 3593.0 22 3851.0 23 4613.5 24 5376.0 25 6054.5 26 8048.0 27 9363.0 28 11204.5 29 13046.0 30 15181.0 31 17316.0 32 19625.0 33 21934.0 34 25627.0 35 29320.0 36 30721.0 37 32122.0 38 35630.0 39 39138.0 40 41813.5 41 44489.0 42 49184.5 43 53880.0 44 59430.0 45 64980.0 46 98116.0 47 131252.0 48 115016.5 49 98781.0 50 98927.0 51 99073.0 52 88291.5 53 77510.0 54 71491.0 55 65472.0 56 61859.0 57 58246.0 58 55489.5 59 52733.0 60 49633.5 61 46534.0 62 42647.0 63 38760.0 64 35026.5 65 31293.0 66 26311.5 67 21330.0 68 18230.5 69 15131.0 70 12955.5 71 10780.0 72 8986.0 73 7192.0 74 5900.0 75 3535.0 76 2462.0 77 1940.0 78 1418.0 79 1070.5 80 723.0 81 533.0 82 343.0 83 274.0 84 205.0 85 124.0 86 43.0 87 28.0 88 13.0 89 11.5 90 10.0 91 7.0 92 4.0 93 5.5 94 7.0 95 3.5 96 0.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1153320.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.353300143522784 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92714772023375 23.17860051227035 2 8.79456062643276 5.514769979019083 3 3.319540647889881 3.1223566281574655 4 1.8618378232770632 2.3349904036707554 5 1.3008347846809054 2.0392731720617627 6 1.0283780557957005 1.9345827506625013 7 0.8658316941408393 1.9002676686120787 8 0.724326025903309 1.8168009033529207 9 0.6757115523078427 1.9067208398958142 >10 7.365499916484914 45.749164928024776 >50 0.09686768852136585 1.9759364833530118 >100 0.03311619385130739 1.8820953616916005 >500 0.0022077462540222316 0.5331680585892057 >1k 0.0027596828175277897 1.629095781745791 >5k 8.279048452583369E-4 1.920339482688872 >10k+ 5.519365635055579E-4 2.5618370462040247 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC 19412 1.6831408455589083 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10196 0.884056463080498 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC 8166 0.7080428675476017 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG 8105 0.7027537890611452 No Hit GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT 5923 0.5135608504144556 No Hit GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC 3811 0.3304373460964867 No Hit GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT 3420 0.29653522006034755 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT 2004 0.17375923421080014 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC 1934 0.16768979988207958 No Hit CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG 1562 0.13543509173516458 No Hit CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC 1550 0.13439461727881247 No Hit CGTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT 1308 0.11341171574237852 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGT 1179 0.1022266153365935 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.734124093920161E-4 0.0 0.0 0.09650400582665696 0.0 2 1.734124093920161E-4 0.0 0.0 0.6394582596330594 0.0 3 1.734124093920161E-4 0.0 0.0 0.9765719834911386 0.0 4 1.734124093920161E-4 0.0 0.0 1.81103249748552 0.0 5 1.734124093920161E-4 0.0 0.0 4.245569312940034 0.0 6 1.734124093920161E-4 0.0 0.0 5.664603058994902 0.0 7 1.734124093920161E-4 0.0 0.0 6.5905386189435715 0.0 8 1.734124093920161E-4 0.0 0.0 7.843356570596192 0.0 9 1.734124093920161E-4 0.0 0.0 8.447698817327367 0.0 10 1.734124093920161E-4 0.0 0.0 10.011185100405784 0.0 11 2.6011861408802413E-4 0.0 0.0 10.825876599729476 0.0 12 2.6011861408802413E-4 0.0 0.0 12.309593174487567 0.0 13 2.6011861408802413E-4 0.0 0.0 12.734713696112093 0.0 14 2.6011861408802413E-4 0.0 0.0 12.98460097804599 0.0 15 2.6011861408802413E-4 0.0 0.0 13.287639163458538 0.0 16 2.6011861408802413E-4 0.0 0.0 13.677990496999966 0.0 17 2.6011861408802413E-4 0.0 0.0 14.08533624666181 0.0 18 2.6011861408802413E-4 0.0 0.0 14.591093538653626 0.0 19 2.6011861408802413E-4 0.0 0.0 14.998525994520168 0.0 20 2.6011861408802413E-4 0.0 0.0 15.33035063989179 0.0 21 2.6011861408802413E-4 0.0 0.0 15.676828633857038 0.0 22 2.6011861408802413E-4 0.0 0.0 16.103856691984877 0.0 23 3.468248187840322E-4 0.0 0.0 16.462040023584088 0.0 24 3.468248187840322E-4 0.0 0.0 16.776350015607118 0.0 25 3.468248187840322E-4 0.0 0.0 17.046006312211702 0.0 26 3.468248187840322E-4 0.0 0.0 17.336992335171505 0.0 27 3.468248187840322E-4 0.0 0.0 17.673672528006104 0.0 28 3.468248187840322E-4 0.0 0.0 17.96890715499601 0.0 29 3.468248187840322E-4 0.0 0.0 18.2970901397704 0.0 30 3.468248187840322E-4 0.0 0.0 18.796431172614714 0.0 31 3.468248187840322E-4 0.0 0.0 19.145683765130233 0.0 32 3.468248187840322E-4 0.0 0.0 19.501786147816738 0.0 33 3.468248187840322E-4 0.0 0.0 19.8458363680505 0.0 34 3.468248187840322E-4 0.0 0.0 20.179481843720737 0.0 35 3.468248187840322E-4 0.0 0.0 20.54451496549093 0.0 36 3.468248187840322E-4 0.0 0.0 20.880674921097352 0.0 37 3.468248187840322E-4 0.0 0.0 21.237644365830818 0.0 38 3.468248187840322E-4 0.0 0.0 21.64906530711338 0.0 39 3.468248187840322E-4 0.0 0.0 22.231557590261158 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 30 2.1655087E-6 45.000004 1 GCACGAA 20 7.033297E-4 45.0 22 CATACCG 20 7.033297E-4 45.0 1 CGTTTTT 4700 0.0 43.18085 1 CTATGCG 60 3.6379788E-12 41.250004 1 TTAACGG 120 0.0 41.250004 2 TTACGCG 35 6.248307E-6 38.57143 1 AATCGTT 60 1.5643309E-10 37.500004 22 CGTATCG 25 0.0021071117 36.0 13 CGTTACC 25 0.0021071117 36.0 23 ATATACG 25 0.0021071117 36.0 1 TCGTATC 25 0.0021071117 36.0 12 TCATCGA 25 0.0021071117 36.0 16 TATAGCG 50 4.8790753E-8 36.0 1 GTACGAA 25 0.0021071117 36.0 12 TACGGCT 1145 0.0 35.9607 7 GAATCTG 2785 0.0 35.305206 1 ACGGCTG 1195 0.0 35.209206 8 CGATGAA 700 0.0 35.035713 19 GTATACG 45 8.6999535E-7 35.000004 1 >>END_MODULE