##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935734.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 372453 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.034855404574536 33.0 31.0 34.0 30.0 34.0 2 32.079365718627585 33.0 31.0 34.0 30.0 34.0 3 32.11200876352184 33.0 31.0 34.0 30.0 34.0 4 35.75130016404754 37.0 35.0 37.0 33.0 37.0 5 34.79683342596247 37.0 35.0 37.0 33.0 37.0 6 35.30569494674496 37.0 35.0 37.0 33.0 37.0 7 35.91601624903008 37.0 35.0 37.0 35.0 37.0 8 35.92552885867479 37.0 35.0 37.0 35.0 37.0 9 37.76957361063007 39.0 38.0 39.0 35.0 39.0 10 37.396237914582514 39.0 37.0 39.0 34.0 39.0 11 37.294117647058826 39.0 37.0 39.0 34.0 39.0 12 37.17666121631454 39.0 37.0 39.0 34.0 39.0 13 37.05794825118874 39.0 37.0 39.0 33.0 39.0 14 38.15216416567996 40.0 37.0 41.0 33.0 41.0 15 38.289730516333606 40.0 37.0 41.0 34.0 41.0 16 38.34629067291712 40.0 37.0 41.0 34.0 41.0 17 38.32738627424131 40.0 37.0 41.0 34.0 41.0 18 38.341447108762715 40.0 37.0 41.0 34.0 41.0 19 38.26947829659044 40.0 37.0 41.0 34.0 41.0 20 38.246745226914534 40.0 37.0 41.0 34.0 41.0 21 38.180771802079725 40.0 37.0 41.0 34.0 41.0 22 38.234988038759255 40.0 37.0 41.0 34.0 41.0 23 38.22168971655484 40.0 37.0 41.0 34.0 41.0 24 38.18862782686674 40.0 37.0 41.0 34.0 41.0 25 38.04071923168829 40.0 36.0 41.0 34.0 41.0 26 38.07515042166394 40.0 36.0 41.0 34.0 41.0 27 38.08426029592996 40.0 36.0 41.0 34.0 41.0 28 37.993907956171654 40.0 36.0 41.0 34.0 41.0 29 37.972455584999985 40.0 36.0 41.0 34.0 41.0 30 37.86077706448867 40.0 36.0 41.0 34.0 41.0 31 37.8166238424714 40.0 36.0 41.0 34.0 41.0 32 37.74234601412796 40.0 36.0 41.0 33.0 41.0 33 37.64027138994719 40.0 36.0 41.0 33.0 41.0 34 37.58334877152285 40.0 36.0 41.0 33.0 41.0 35 37.52160406816431 40.0 36.0 41.0 33.0 41.0 36 37.474685396546676 40.0 36.0 41.0 33.0 41.0 37 37.42244793302779 40.0 35.0 41.0 33.0 41.0 38 37.350594571664075 40.0 35.0 41.0 33.0 41.0 39 37.322907856830255 40.0 35.0 41.0 33.0 41.0 40 37.23001291438114 40.0 35.0 41.0 32.0 41.0 41 37.21914711386403 40.0 35.0 41.0 33.0 41.0 42 37.24102101473206 40.0 35.0 41.0 33.0 41.0 43 37.14569086569312 39.0 35.0 41.0 32.0 41.0 44 37.03587029772884 39.0 35.0 41.0 32.0 41.0 45 36.99054914311336 39.0 35.0 41.0 32.0 41.0 46 36.88704346588697 39.0 35.0 41.0 32.0 41.0 47 36.80588960217799 39.0 35.0 41.0 31.0 41.0 48 36.80066746676762 39.0 35.0 41.0 31.0 41.0 49 36.81862140994971 39.0 35.0 41.0 32.0 41.0 50 36.70279203013535 39.0 35.0 41.0 31.0 41.0 51 35.60034688940618 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 2.0 10 3.0 11 6.0 12 2.0 13 3.0 14 4.0 15 10.0 16 20.0 17 33.0 18 64.0 19 125.0 20 234.0 21 353.0 22 659.0 23 930.0 24 1280.0 25 1768.0 26 2255.0 27 2874.0 28 3151.0 29 3806.0 30 4758.0 31 6109.0 32 8281.0 33 12243.0 34 22524.0 35 32851.0 36 25191.0 37 37525.0 38 70080.0 39 135206.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.46980424375693 19.665031561029178 24.49517120280948 15.369992992404411 2 27.726451391182245 26.756127618786802 27.800554700861586 17.71686628916937 3 30.066075451130747 26.17296679044067 27.633553763830605 16.127403994597977 4 27.2254485800893 26.871041446840273 27.76484549728422 18.138664475786207 5 25.2480178707112 31.17950452808811 25.380115074922205 18.192362526278483 6 24.448990879386123 35.744644290689024 24.450870311153352 15.355494518771495 7 80.24341326288149 5.434511200070881 9.808217412666833 4.5138581243807945 8 82.26111751012878 5.173001694173493 7.388583257484838 5.177297538212875 9 75.96314165814209 6.959535834051545 10.006363218983335 7.07095928882302 10 38.61587905051107 33.428110392452204 15.362475265335492 12.593535291701235 11 28.48305692261842 23.30978673819247 29.24718018112352 18.95997615806558 12 25.946897997868184 20.300279498352815 32.299914351609466 21.452908152169535 13 22.001433737948144 25.45448687485401 34.70370758189624 17.840371805301608 14 18.774180903362303 30.381551497772875 29.856921544463326 20.987346054401495 15 15.683589607279307 26.65383283259901 39.47236295586289 18.19021460425879 16 18.931516191304677 25.7090156341874 34.03704628503462 21.322421889473304 17 18.35453063876516 25.95065686140265 29.50627327474876 26.188539225083435 18 20.035011128920964 25.098200309837754 32.863743881778376 22.003044679462914 19 21.689716554840476 27.388690653585822 28.987013126488442 21.93457966508526 20 23.033510268409703 26.627520787857794 29.3822307781116 20.956738165620898 21 21.41693045833971 28.525746872759782 29.957336898883884 20.09998577001662 22 20.44553272493442 25.374476779620515 29.030240057134726 25.149750438310335 23 18.051136653483795 28.349617267145117 29.210665506788775 24.388580572582313 24 19.992858159284527 25.253119185507973 31.824686604752817 22.929336050454687 25 18.12174958988114 28.574075118202835 30.136688387528093 23.167486904387935 26 17.958239026132155 28.53245912907132 29.310006900199486 24.199294944597035 27 19.117042955755494 30.193876811302367 28.12784431861201 22.56123591433013 28 18.278010916813663 26.904602728397947 32.6779486270751 22.139437727713297 29 19.4910498774342 25.579603332501012 30.43498105801269 24.4943657320521 30 20.85202696716096 28.327064085938357 29.189186286591866 21.631722660308817 31 23.28293771294633 28.24248965641302 24.43798277903521 24.036589851605438 32 22.229113472035397 29.771004663675686 24.88475055913095 23.115131305157966 33 22.88879402233302 28.11012396194956 25.932668014487735 23.068414001229684 34 20.865719970036487 26.243042746333096 27.694501051139337 25.19673623249108 35 19.562468284588928 28.67100009934139 26.615170236244573 25.151361379825104 36 22.570364582913818 27.13845773829181 27.673021830942428 22.618155847851945 37 20.389418262169993 28.302631472964375 29.33792988645547 21.97002037841016 38 21.46901756731722 29.62789935911377 26.9000383941061 22.003044679462914 39 20.665157751447833 26.63127965139226 29.934783717677128 22.76877887948278 40 22.141048669228063 26.17967904675221 28.734095308669822 22.94517697534991 41 18.38594399830314 26.052146176833052 28.507489535592413 27.0544202892714 42 20.8211505881279 26.215388250329575 29.076957361063005 23.886503800479524 43 22.336778063272416 25.56000354407133 28.883644379290807 23.219574013365445 44 21.687568632820785 26.255393297946316 28.762823765683187 23.29421430354971 45 20.70945864310396 26.696791272992833 26.64363020300548 25.950119880897727 46 23.51357083981066 26.673164130776232 27.463330943770085 22.34993408564302 47 19.282164461019242 25.382262996941897 31.27079121392498 24.06478132811388 48 20.46110515957718 25.53154357731043 29.598365431343016 24.408985831769378 49 21.435187795507087 22.71615479000035 32.098278172010964 23.7503792424816 50 20.575482007125732 24.003028570047764 30.520092468042947 24.901396954783557 51 19.281895970766783 24.593438635210347 28.12113206230048 28.00353333172239 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 147.0 1 295.5 2 444.0 3 915.0 4 1386.0 5 945.0 6 504.0 7 533.5 8 563.0 9 576.0 10 589.0 11 594.0 12 599.0 13 633.0 14 667.0 15 645.5 16 624.0 17 606.0 18 588.0 19 753.0 20 918.0 21 996.0 22 1074.0 23 1245.0 24 1416.0 25 1844.0 26 2924.5 27 3577.0 28 4152.5 29 4728.0 30 5906.0 31 7084.0 32 7899.5 33 8715.0 34 10025.5 35 11336.0 36 11862.0 37 12388.0 38 13359.0 39 14330.0 40 15675.5 41 17021.0 42 21124.0 43 25227.0 44 28365.0 45 31503.0 46 37452.5 47 43402.0 48 43701.5 49 44001.0 50 42683.5 51 41366.0 52 35527.0 53 29688.0 54 24226.5 55 18765.0 56 16010.0 57 13255.0 58 12077.0 59 10899.0 60 10035.5 61 9172.0 62 7283.5 63 5395.0 64 4586.0 65 3777.0 66 2808.5 67 1840.0 68 1509.0 69 1178.0 70 1010.0 71 842.0 72 704.5 73 567.0 74 455.0 75 243.5 76 144.0 77 102.5 78 61.0 79 45.0 80 29.0 81 23.0 82 17.0 83 12.5 84 8.0 85 5.5 86 3.0 87 1.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 372453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.81690085572351 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69065627602225 24.182989610034213 2 10.913218098947633 7.162759927401037 3 3.942951981869499 3.881863928036696 4 1.920772369414681 2.521351856539788 5 1.1225037108633145 1.841854649479156 6 0.7686766402188812 1.5135351055304198 7 0.5997834238706322 1.377812320924825 8 0.4735846856703966 1.2433265341147506 9 0.4547362667793949 1.3430731484161111 >10 5.772269959244366 43.933046762349385 >50 0.28840865062220505 5.7624294380595495 >100 0.04588319441716899 2.5662184012337432 >500 0.0024580282723483384 0.565998505301449 >1k 0.00409671378724723 2.1037398125788775 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC 1805 0.4846249056927988 No Hit GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1626 0.436565150502211 No Hit GAATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTC 1549 0.41589140106268446 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG 1534 0.41186404727576365 No Hit CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1310 0.35172223072441355 No Hit CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT 800 0.21479220196910753 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 773 0.20754296515265014 No Hit TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 532 0.1428368143094565 No Hit ACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 395 0.10605364972224685 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.684902524613844E-4 0.0 0.0 0.23788236368078658 0.0 2 2.684902524613844E-4 0.0 0.0 1.3631250117464486 0.0 3 2.684902524613844E-4 0.0 0.0 1.8187529701734177 0.0 4 2.684902524613844E-4 0.0 0.0 2.498301799153182 0.0 5 2.684902524613844E-4 0.0 0.0 4.310611003267526 0.0 6 2.684902524613844E-4 0.0 0.0 5.4200127264379665 0.0 7 2.684902524613844E-4 0.0 0.0 6.4767903601259755 0.0 8 2.684902524613844E-4 0.0 0.0 8.351657793063822 0.0 9 2.684902524613844E-4 0.0 0.0 9.10745785374262 0.0 10 2.684902524613844E-4 0.0 0.0 10.910906879525738 0.0 11 2.684902524613844E-4 0.0 0.0 12.51325670621528 0.0 12 2.684902524613844E-4 0.0 0.0 14.446923504442172 0.0 13 2.684902524613844E-4 0.0 0.0 15.014511898145537 0.0 14 2.684902524613844E-4 0.0 0.0 15.242728612737714 0.0 15 2.684902524613844E-4 0.0 0.0 15.646537952439637 0.0 16 2.684902524613844E-4 0.0 0.0 16.35884259221969 0.0 17 2.684902524613844E-4 0.0 0.0 17.315473361739603 0.0 18 2.684902524613844E-4 0.0 0.0 18.56046266240304 0.0 19 2.684902524613844E-4 0.0 0.0 19.310624427780148 0.0 20 2.684902524613844E-4 0.0 0.0 19.99393212029437 0.0 21 2.684902524613844E-4 0.0 0.0 20.725568058251646 0.0 22 2.684902524613844E-4 0.0 0.0 21.489959807009207 0.0 23 2.684902524613844E-4 0.0 0.0 22.300800369442587 0.0 24 2.684902524613844E-4 0.0 0.0 22.88288723677887 0.0 25 5.369805049227688E-4 0.0 0.0 23.39087079443581 0.0 26 5.369805049227688E-4 0.0 0.0 23.867709482807225 0.0 27 5.369805049227688E-4 0.0 0.0 24.366295881628016 0.0 28 5.369805049227688E-4 0.0 0.0 24.88850942266541 0.0 29 5.369805049227688E-4 0.0 0.0 25.417435220014337 0.0 30 5.369805049227688E-4 0.0 0.0 26.077384260564422 0.0 31 8.054707573841532E-4 0.0 0.0 26.615975707001958 0.0 32 0.0010739610098455376 0.0 0.0 27.126912657435973 0.0 33 0.0010739610098455376 0.0 0.0 27.608584170351694 0.0 34 0.0010739610098455376 0.0 0.0 28.109050000939718 0.0 35 0.0010739610098455376 0.0 0.0 28.690868378023534 0.0 36 0.0010739610098455376 0.0 0.0 29.18435346204756 0.0 37 0.0010739610098455376 0.0 0.0 29.659312718651748 0.0 38 0.0010739610098455376 0.0 0.0 30.12863367995425 0.0 39 0.0010739610098455376 0.0 0.0 30.607351800092896 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGG 30 2.161536E-6 45.000004 2 TCCGGCT 25 3.885711E-5 45.0 27 TCGCATC 20 7.027139E-4 45.0 36 TACCCCC 20 7.027139E-4 45.0 26 ACGCCGA 20 7.027139E-4 45.0 12 TCCGCGG 20 7.027139E-4 45.0 2 CGAAATG 25 3.885711E-5 45.0 21 GGTCGAT 25 3.885711E-5 45.0 9 CGACCTA 20 7.027139E-4 45.0 30 CCGCTCG 20 7.027139E-4 45.0 19 ACGGGTA 25 3.885711E-5 45.0 5 ACGATTG 20 7.027139E-4 45.0 1 GCCGACA 20 7.027139E-4 45.0 26 AGGTTAC 25 3.885711E-5 45.0 15 AGTTGCG 20 7.027139E-4 45.0 1 AACGCGA 20 7.027139E-4 45.0 34 GACGGTC 25 3.885711E-5 45.0 29 GCGGCAT 20 7.027139E-4 45.0 18 CGTGTCA 20 7.027139E-4 45.0 22 CGCAAGT 25 3.885711E-5 45.0 33 >>END_MODULE