SRR2935733.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043787
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	20615	1.975019807681069	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	12367	1.1848202746345757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	11472	1.0990748112402244	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	6983	0.6690062244500075	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	6054	0.5800033914965409	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.40190192060257507	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	3267	0.3129948926361413	RNA PCR Primer, Index 38 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	2985	0.2859778862928931	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	2697	0.25838605002744813	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2673	0.2560867303386611	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1984	0.1900770942730653	RNA PCR Primer, Index 38 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	1966	0.18835260450647498	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1852	0.17743083598473636	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	1344	0.1287619025720765	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1140	0.1092176852173863	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACG	35	1.2117926E-7	45.000004	1
CGGCGGA	20	7.0329895E-4	45.0	31
CCTACGC	20	7.0329895E-4	45.0	42
ATCCGGT	20	7.0329895E-4	45.0	3
CGTAGAT	20	7.0329895E-4	45.0	44
CTCGATC	20	7.0329895E-4	45.0	16
AGTGTCG	45	3.8562575E-10	45.0	1
CTCCGAC	30	2.1653104E-6	44.999996	43
CGTTTTT	3455	0.0	43.371925	1
TCGACGG	65	0.0	41.53846	2
CTGTACG	40	3.457808E-7	39.375	1
CGTAAGG	160	0.0	39.375	2
TGTCGAG	40	3.457808E-7	39.375	1
TAGTACG	40	3.457808E-7	39.375	1
GCGATTA	120	0.0	39.374996	9
CGATTGA	75	0.0	39.0	10
AAGGGCG	390	0.0	38.653847	5
CAGCGCG	35	6.2477357E-6	38.57143	1
CACGGTA	35	6.2477357E-6	38.57143	4
GAATGCG	35	6.2477357E-6	38.57143	1