##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043787
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851936266690426	33.0	31.0	34.0	30.0	34.0
2	31.901747195548516	33.0	31.0	34.0	30.0	34.0
3	31.92301398657006	33.0	31.0	34.0	30.0	34.0
4	35.62759164465547	37.0	35.0	37.0	33.0	37.0
5	34.673642227772525	37.0	35.0	37.0	33.0	37.0
6	35.14206442502158	37.0	35.0	37.0	32.0	37.0
7	35.75025555980291	37.0	35.0	37.0	33.0	37.0
8	35.70044463094482	37.0	35.0	37.0	35.0	37.0
9	37.54527216759741	39.0	37.0	39.0	35.0	39.0
10	37.10823089385095	39.0	37.0	39.0	33.0	39.0
11	37.07371044092329	39.0	37.0	39.0	33.0	39.0
12	37.15338665838912	39.0	37.0	39.0	34.0	39.0
13	37.121158818801156	39.0	37.0	39.0	33.0	39.0
14	38.28153732514392	40.0	38.0	41.0	33.0	41.0
15	38.29403412765248	40.0	38.0	41.0	33.0	41.0
16	38.34585600318839	40.0	38.0	41.0	34.0	41.0
17	38.27632265969973	40.0	37.0	41.0	34.0	41.0
18	38.21644166865462	40.0	37.0	41.0	34.0	41.0
19	38.09338591111022	40.0	37.0	41.0	34.0	41.0
20	38.002640385442625	40.0	37.0	41.0	33.0	41.0
21	38.049391303014886	40.0	37.0	41.0	34.0	41.0
22	38.10381524199861	40.0	37.0	41.0	34.0	41.0
23	38.08142274237943	40.0	37.0	41.0	34.0	41.0
24	38.05291501043796	40.0	37.0	41.0	34.0	41.0
25	37.9399638048759	40.0	37.0	41.0	33.0	41.0
26	37.93682140130122	40.0	37.0	41.0	33.0	41.0
27	37.92002199682502	40.0	37.0	41.0	33.0	41.0
28	37.80260340471763	40.0	36.0	41.0	33.0	41.0
29	37.73886913709406	40.0	36.0	41.0	33.0	41.0
30	37.61438875939248	40.0	36.0	41.0	33.0	41.0
31	37.56074371495334	40.0	36.0	41.0	33.0	41.0
32	37.462218824338684	40.0	36.0	41.0	33.0	41.0
33	37.252447098881284	40.0	36.0	41.0	32.0	41.0
34	37.17055874426487	40.0	36.0	41.0	31.0	41.0
35	37.05685546955461	40.0	36.0	41.0	31.0	41.0
36	37.00469444436461	40.0	36.0	41.0	31.0	41.0
37	36.9424758116359	40.0	36.0	41.0	31.0	41.0
38	36.9000332443305	40.0	36.0	41.0	31.0	41.0
39	36.84384266138589	40.0	36.0	41.0	31.0	41.0
40	36.725757266568756	40.0	36.0	41.0	30.0	41.0
41	36.613591661900365	39.0	35.0	41.0	30.0	41.0
42	36.68978441003768	39.0	36.0	41.0	30.0	41.0
43	36.593286752948636	39.0	35.0	41.0	30.0	41.0
44	36.50356059234307	39.0	35.0	41.0	30.0	41.0
45	36.48529153936579	39.0	35.0	41.0	30.0	41.0
46	36.345634693668345	39.0	35.0	41.0	29.0	41.0
47	36.17188564333528	39.0	35.0	41.0	29.0	41.0
48	36.16844528625093	39.0	35.0	41.0	29.0	41.0
49	36.16151954373833	39.0	35.0	41.0	29.0	41.0
50	36.016216910155045	39.0	35.0	41.0	28.0	41.0
51	34.79801147168915	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	11.0
11	9.0
12	12.0
13	12.0
14	17.0
15	28.0
16	51.0
17	101.0
18	253.0
19	612.0
20	1241.0
21	2216.0
22	3109.0
23	4297.0
24	5652.0
25	7900.0
26	10242.0
27	11184.0
28	11812.0
29	12930.0
30	15875.0
31	20757.0
32	26957.0
33	35950.0
34	54359.0
35	72517.0
36	76004.0
37	110805.0
38	204440.0
39	354202.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5561086696807	17.76511874549118	22.347567080256795	16.331205504571336
2	28.20795813705287	30.327260255205324	25.176113517413036	16.288668090328773
3	27.336515975002563	29.693989290918548	28.022479682157375	14.947015051921515
4	24.92251771673723	23.715183270149947	33.85796144232492	17.50433757078791
5	26.5884706362505	29.153744969040616	26.489599889632654	17.768184505076228
6	22.924696322142353	35.983011859699346	27.236782983501424	13.855508834656879
7	75.80167218024367	4.72136556596317	15.194862553375355	4.282099700417805
8	76.12434337657012	7.551923907847099	10.754301404405304	5.569431311177472
9	69.82688996893044	6.398719278933346	12.649132437939926	11.125258314196287
10	34.269922886565936	27.283823232134523	22.016752460032553	16.42950142126698
11	25.303629955153685	24.85258007620329	30.639488707945205	19.20430126069782
12	21.352919704882318	21.08868955064587	35.25048692884659	22.307903815625217
13	21.947389649420813	23.943199139287998	38.01618529450932	16.093225916781872
14	19.711013837114276	29.49615199269583	31.390599806282317	19.402234363907578
15	17.556359678746716	26.607823243631124	38.17713767272441	17.658679404897743
16	19.677003066717635	27.147301125612795	30.7826213585722	22.393074449097373
17	20.744174817275937	26.933560199542626	30.128273297138207	22.193991686043223
18	21.775419697696943	25.604074394488528	31.358217720665234	21.262288187149295
19	20.0686538537077	28.883766515582206	28.8904728646745	22.1571067660356
20	20.864505880989128	27.338815294691347	32.143435394386024	19.6532434299335
21	20.394390809619203	30.18182828488954	31.110945049133587	18.31283585635767
22	18.79051952170318	25.18990943554576	31.86521771204278	24.15435333070828
23	18.232455472237152	28.854258579576104	31.08459867769957	21.82868727048718
24	20.346488316102807	26.222016560850058	29.988972845992524	23.442522277054607
25	18.066617039683383	31.617466015575975	28.378299403997175	21.937617540743464
26	19.42695205056204	26.740800565632643	30.71517464770111	23.117072736104205
27	21.9254503073903	27.901094763586826	28.009066983972787	22.164387945050095
28	20.121538206549804	26.53817301805828	31.541109440910837	21.799179334481078
29	22.99013112828575	25.02378358803089	28.806930915981898	23.179154367701457
30	21.48120258251923	29.02546209140371	29.210174106402935	20.283161219674128
31	23.74517023109121	27.20967017217114	26.24663844251749	22.79852115422016
32	22.61103079459698	27.770225151300025	27.61205111770888	22.006692936394114
33	20.85253025761003	27.096620287472444	28.61694962669587	23.43389982822166
34	22.72542194911414	27.351365747992645	28.618674116462454	21.304538186430758
35	20.03675079302578	27.30221778964482	30.099531801028373	22.561499616301024
36	25.95376259715823	26.36361633168453	28.554580580137518	19.128040491019718
37	20.573067110435368	28.251932626100917	30.48792521845932	20.687075045004395
38	21.70711074194256	26.23198027950147	29.74610720386439	22.31480177469158
39	22.639484875745723	26.077159420456475	28.82906186798648	22.454293835811328
40	20.711505316697757	25.320683242845522	33.65610033464682	20.311711105809902
41	20.868817105405604	28.19272514411465	28.79035665322523	22.148101097254518
42	22.14723885237122	25.024071002991988	32.09495807094743	20.733732073689364
43	24.64018041995158	24.541980308242966	28.537335682471614	22.28050358933384
44	22.00736357130334	25.064500707519827	28.844390665911725	24.08374505526511
45	20.70441574765733	24.043985985646497	28.832798262480758	26.41880000421542
46	24.63471953569071	26.282182092706652	27.988660521734797	21.094437849867838
47	19.684092635758063	24.78369629052671	33.653226185035834	21.878984888679394
48	21.11953875647043	24.922805131698325	29.791901987666066	24.16575412416518
49	21.322262109031822	22.25310336304246	33.38420578144775	23.04042874647797
50	21.050846580767914	22.731170248336106	32.273634371763585	23.94434879913239
51	20.235450336131798	22.22091288739944	29.02459984652041	28.51903692994835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	449.0
2	702.0
3	4269.0
4	7836.0
5	5547.5
6	3259.0
7	3190.0
8	3121.0
9	3100.0
10	3079.0
11	3069.0
12	3059.0
13	3039.5
14	3020.0
15	3108.0
16	3196.0
17	2960.5
18	2725.0
19	2759.5
20	2794.0
21	3135.5
22	3477.0
23	3945.0
24	4413.0
25	5539.5
26	7956.5
27	9247.0
28	10599.0
29	11951.0
30	14502.5
31	17054.0
32	20576.0
33	24098.0
34	27149.5
35	30201.0
36	31867.5
37	33534.0
38	38118.0
39	42702.0
40	46924.0
41	51146.0
42	57203.0
43	63260.0
44	71971.0
45	80682.0
46	123204.5
47	165727.0
48	144446.5
49	123166.0
50	117257.0
51	111348.0
52	93225.0
53	75102.0
54	63674.5
55	52247.0
56	43980.5
57	35714.0
58	30642.0
59	25570.0
60	21844.0
61	18118.0
62	15087.0
63	12056.0
64	9291.5
65	6527.0
66	4905.0
67	3283.0
68	2434.0
69	1585.0
70	1194.0
71	803.0
72	610.5
73	418.0
74	394.0
75	267.5
76	165.0
77	137.0
78	109.0
79	67.0
80	25.0
81	19.0
82	13.0
83	10.5
84	8.0
85	5.5
86	3.0
87	4.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1043787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.28127063621681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.567219930389	28.92809197381738
2	10.21305894854412	7.819377472657066
3	3.779452482101979	4.340467299722016
4	1.9919558668054382	3.050184065303153
5	1.3006777524055075	2.489579852517071
6	0.9199729215007628	2.1130639431577043
7	0.7218920694601428	1.9344461976799645
8	0.5887355114858552	1.8030034758673243
9	0.49296209007141223	1.6984093665076936
>10	4.266966232867867	32.24526153319747
>50	0.11789309327738691	2.9227879192101867
>100	0.03271921174560983	2.2120115217350174
>500	0.002497649748036761	0.545760179614394
>1k	0.0027474147228404365	2.4006563752204286
>5k	4.995299496073521E-4	1.246509366088447
>10k+	7.492949244110283E-4	4.250389457704673
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	20615	1.975019807681069	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	12367	1.1848202746345757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	11472	1.0990748112402244	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	6983	0.6690062244500075	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	6054	0.5800033914965409	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.40190192060257507	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	3267	0.3129948926361413	RNA PCR Primer, Index 38 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	2985	0.2859778862928931	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	2697	0.25838605002744813	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2673	0.2560867303386611	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1984	0.1900770942730653	RNA PCR Primer, Index 38 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	1966	0.18835260450647498	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1852	0.17743083598473636	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	1344	0.1287619025720765	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1140	0.1092176852173863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.664398962623601E-4	0.0	0.0	0.1994659830022792	0.0
2	7.664398962623601E-4	0.0	0.0	1.2382794573988753	0.0
3	7.664398962623601E-4	0.0	0.0	1.7461416936597218	0.0
4	7.664398962623601E-4	0.0	0.0	2.938626367256921	0.0
5	7.664398962623601E-4	0.0	0.0	6.1140826624589115	0.0
6	7.664398962623601E-4	0.0	0.0	7.724756104454261	0.0
7	7.664398962623601E-4	0.0	0.0	9.032110957503782	0.0
8	7.664398962623601E-4	0.0	0.0	10.976856389282487	0.0
9	7.664398962623601E-4	0.0	0.0	11.760924403158882	0.0
10	7.664398962623601E-4	0.0	0.0	14.306367103633212	0.0
11	7.664398962623601E-4	0.0	0.0	15.80245778113734	0.0
12	7.664398962623601E-4	0.0	0.0	18.443609663657433	0.0
13	7.664398962623601E-4	0.0	0.0	19.18772699794115	0.0
14	7.664398962623601E-4	0.0	0.0	19.56031259251169	0.0
15	7.664398962623601E-4	0.0	0.0	20.12958582546056	0.0
16	7.664398962623601E-4	0.0	0.0	20.923042728066168	0.0
17	7.664398962623601E-4	0.0	0.0	21.910792144374284	0.0
18	7.664398962623601E-4	0.0	0.0	23.126461624833418	0.0
19	7.664398962623601E-4	0.0	0.0	24.003173061170525	0.0
20	7.664398962623601E-4	0.0	0.0	24.722189488851654	0.0
21	7.664398962623601E-4	0.0	0.0	25.49255738958236	0.0
22	7.664398962623601E-4	0.0	0.0	26.34033571983556	0.0
23	7.664398962623601E-4	0.0	0.0	27.148738200418286	0.0
24	7.664398962623601E-4	0.0	0.0	27.766297146831683	0.0
25	7.664398962623601E-4	0.0	0.0	28.3561684519926	0.0
26	7.664398962623601E-4	0.0	0.0	28.927357784682123	0.0
27	7.664398962623601E-4	0.0	0.0	29.557467184396817	0.0
28	7.664398962623601E-4	0.0	0.0	30.142548240206096	0.0
29	7.664398962623601E-4	0.0	0.0	30.755508547241917	0.0
30	7.664398962623601E-4	0.0	0.0	31.57607826117781	0.0
31	9.580498703279501E-4	0.0	0.0	32.23224661736542	0.0
32	0.001053854857360745	0.0	0.0	32.85679932783221	0.0
33	0.001053854857360745	0.0	0.0	33.454718251903884	0.0
34	0.00114965984439354	0.0	0.0	34.03548808329669	0.0
35	0.00114965984439354	0.0	0.0	34.66588489797248	0.0
36	0.00114965984439354	0.0	0.0	35.22816436686795	0.0
37	0.00114965984439354	0.0	0.0	35.8134370326513	0.0
38	0.00114965984439354	0.0	0.0	36.396697793706956	0.0
39	0.001245464831426335	0.0	0.0	36.983215924321726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACG	35	1.2117926E-7	45.000004	1
CGGCGGA	20	7.0329895E-4	45.0	31
CCTACGC	20	7.0329895E-4	45.0	42
ATCCGGT	20	7.0329895E-4	45.0	3
CGTAGAT	20	7.0329895E-4	45.0	44
CTCGATC	20	7.0329895E-4	45.0	16
AGTGTCG	45	3.8562575E-10	45.0	1
CTCCGAC	30	2.1653104E-6	44.999996	43
CGTTTTT	3455	0.0	43.371925	1
TCGACGG	65	0.0	41.53846	2
CTGTACG	40	3.457808E-7	39.375	1
CGTAAGG	160	0.0	39.375	2
TGTCGAG	40	3.457808E-7	39.375	1
TAGTACG	40	3.457808E-7	39.375	1
GCGATTA	120	0.0	39.374996	9
CGATTGA	75	0.0	39.0	10
AAGGGCG	390	0.0	38.653847	5
CAGCGCG	35	6.2477357E-6	38.57143	1
CACGGTA	35	6.2477357E-6	38.57143	4
GAATGCG	35	6.2477357E-6	38.57143	1
>>END_MODULE